FastQCFastQC Report
Mon 15 Aug 2016
SRR2921642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515076
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.16137804308166717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.133062631841571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.059.920431
GTATCAA38000.054.147231
TCAACGC48400.041.6442074
CAACGCA49150.041.0852055
ATCAACG50500.040.031873
TATCAAC51100.039.4451332
AACGCAG51800.039.2289056
GTACATG77750.035.1322361
TACATGG80000.033.4698642
ACATGGG79850.032.9352723
ACGCAGA62800.031.8810427
CATGGGG72200.031.5507324
ATGGGGG41050.031.1985635
CGCAGAG67050.029.857288
GTGGTAT12600.029.8202111
GCAGAGT76250.026.0985539
TACACCG2851.1641532E-1025.081055
TGGTATC13950.023.93932
AGAGTAC67800.023.68311910-11
CAGAGTA71200.022.80322810-11
Produced by FastQC (version 0.11.5)