##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921642_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1515076 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83871370149088 33.0 31.0 34.0 30.0 34.0 2 32.00791445445641 34.0 31.0 34.0 30.0 34.0 3 32.06725867217222 34.0 31.0 34.0 30.0 34.0 4 35.473466017546315 37.0 35.0 37.0 35.0 37.0 5 35.33648147023648 37.0 35.0 37.0 33.0 37.0 6 35.36631693723615 37.0 35.0 37.0 33.0 37.0 7 35.258070882252774 37.0 35.0 37.0 33.0 37.0 8 35.30120733217343 37.0 36.0 37.0 33.0 37.0 9 37.03778754333116 39.0 38.0 39.0 34.0 39.0 10-11 36.9881421790062 39.0 38.0 39.0 34.0 39.0 12-13 36.91858461225708 39.0 38.0 39.0 34.0 39.0 14-15 38.346453907262735 41.0 39.0 41.0 34.0 41.0 16-17 38.35932421871907 41.0 39.0 41.0 34.0 41.0 18-19 38.368274264789356 41.0 39.0 41.0 34.0 41.0 20-21 38.305533187774074 41.0 39.0 41.0 34.0 41.0 22-23 38.309568628900465 41.0 39.0 41.0 34.0 41.0 24-25 38.26616915587073 41.0 39.0 41.0 34.0 41.0 26-27 38.19153032587144 41.0 39.0 41.0 34.0 41.0 28-29 38.1217829996647 41.0 39.0 41.0 34.0 41.0 30-31 38.00591851497879 41.0 38.5 41.0 33.5 41.0 32-33 37.885455581106164 41.0 38.0 41.0 33.0 41.0 34-35 37.753246041782724 41.0 38.0 41.0 33.0 41.0 36-37 37.67728153571174 40.0 38.0 41.0 33.0 41.0 38-39 37.59058489475115 40.0 38.0 41.0 33.0 41.0 40-41 37.46491694146036 40.0 38.0 41.0 32.5 41.0 42-43 37.29913977912659 40.0 38.0 41.0 32.0 41.0 44-45 37.13469852337441 40.0 37.0 41.0 31.5 41.0 46-47 36.91778267228838 40.0 37.0 41.0 31.0 41.0 48-49 36.71632644170985 40.0 37.0 41.0 31.0 41.0 50-51 36.462493960699 40.0 36.0 41.0 30.0 41.0 52-53 36.32713408436277 40.0 36.0 41.0 30.0 41.0 54-55 36.194848971272734 40.0 35.0 41.0 30.0 41.0 56-57 35.993156778933866 39.0 35.0 41.0 30.0 41.0 58-59 35.79452548915038 39.0 35.0 41.0 29.5 41.0 60-61 35.54004122565469 38.5 35.0 41.0 29.0 41.0 62-63 35.18624742257154 37.5 35.0 40.5 28.5 41.0 64-65 34.89117146598586 37.0 35.0 40.0 28.5 41.0 66-67 34.58966348882828 36.5 35.0 39.5 29.0 41.0 68-69 34.2251193999509 36.0 35.0 39.0 28.0 41.0 70-71 33.85470035826586 35.5 35.0 39.0 28.0 41.0 72-73 33.53872512005998 35.0 34.0 37.5 28.0 40.0 74-75 33.22305679715077 35.0 34.0 37.0 27.0 39.0 76-77 32.903657308280245 35.0 34.0 36.5 27.0 39.0 78-79 32.65018355514839 35.0 34.0 36.0 26.5 38.0 80-81 32.40792574101893 35.0 34.0 36.0 26.0 37.0 82-83 32.19622976009124 35.0 34.0 35.0 26.0 37.0 84-85 31.99111397712062 35.0 34.0 35.0 25.5 36.0 86-87 31.871422951719914 35.0 34.0 35.0 25.0 36.0 88-89 31.777825666831234 35.0 34.0 35.0 25.0 36.0 90-91 31.679976780042715 35.0 34.0 35.0 25.0 35.5 92-93 31.605479197083184 35.0 33.5 35.0 25.0 35.0 94-95 31.539196713564202 35.0 33.0 35.0 25.0 35.0 96-97 31.472374323136265 35.0 33.0 35.0 24.5 35.0 98-99 31.403195285253016 35.0 33.0 35.0 24.0 35.0 100-101 31.33355950460571 35.0 33.0 35.0 24.0 35.0 102-103 30.64768335053819 34.0 31.5 35.0 23.0 35.0 104-105 31.16893178956039 35.0 33.0 35.0 23.5 35.0 106-107 31.273642378336135 35.0 33.0 35.0 24.0 35.0 108-109 31.259431870084406 35.0 33.0 35.0 24.0 35.0 110-111 31.193494913786502 35.0 33.0 35.0 23.0 35.0 112-113 31.154401825386977 35.0 33.0 35.0 23.0 35.0 114-115 31.02947409898909 35.0 33.0 35.0 20.0 35.0 116-117 31.217542882337256 35.0 33.5 35.0 23.0 35.0 118-119 31.203795057145648 35.0 34.0 35.0 23.0 35.0 120-121 31.125464333142364 35.0 34.0 35.0 21.5 35.0 122-123 30.995869514136587 35.0 33.0 35.0 19.0 35.0 124-125 30.359814953177263 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21605.0 3 5870.0 4 3298.0 5 2637.0 6 2811.0 7 3079.0 8 3377.0 9 3610.0 10 3976.0 11 4194.0 12 4997.0 13 6450.0 14 8709.0 15 8071.0 16 4506.0 17 3830.0 18 3537.0 19 3083.0 20 3007.0 21 3158.0 22 3364.0 23 4177.0 24 5513.0 25 7057.0 26 8789.0 27 10252.0 28 12667.0 29 15576.0 30 18760.0 31 23692.0 32 30779.0 33 42429.0 34 66108.0 35 119062.0 36 278557.0 37 567304.0 38 196911.0 39 274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05993474675747 17.64887628951736 10.988543051691606 27.302645912033558 2 16.28997893136059 19.50987691479082 36.8750693047349 27.32507484911369 3 19.154589037071077 22.927391608350053 28.21388414058843 29.70413521399044 4 13.679177611070967 14.683627655994535 33.62378840238032 38.013406330554176 5 14.979818025533465 36.92060172256486 32.384245780402686 15.715334471498984 6 35.39996776903608 33.31189005542669 16.053265630696558 15.234876544840667 7 31.125174006873106 31.28696407864186 18.663178164547766 18.924683749937266 8 28.837712058022614 34.157816333915285 17.934436664544876 19.070034943517225 9 28.267167750352574 15.140328961615863 17.948162663807356 38.64434062422421 10-11 27.41009021110899 24.689722854856836 25.698592067405883 22.201594866628298 12-13 27.26902307606457 21.912289425875333 26.42203779685429 24.39664970120581 14-15 24.544406911740914 23.22420231998336 23.80987759495719 28.42151317331854 16-17 24.711981623877865 26.54946176808433 24.478185948949275 24.260370659088533 18-19 24.686382812533527 25.539642090646996 26.410365353830574 23.363609742988903 20-21 25.39136525696502 24.406866044399347 26.3256574853794 23.87611121325623 22-23 25.18505341954033 24.017490019210772 26.273707777804546 24.52374878344435 24-25 24.697674664209472 24.477015617749622 26.2379975802092 24.587312137831706 26-27 24.426765939119715 24.72108500038778 26.47564640129107 24.37650265920143 28-29 24.467478411019343 25.08179819393812 26.304728222736628 24.145995172305906 30-31 24.936082608958237 24.638136975125764 26.236872059280223 24.188908356635775 32-33 24.661951870263447 24.565776888564418 26.267981652163407 24.504289589008724 34-35 24.68070416534242 24.778327057811698 26.34171246658858 24.1992563102573 36-37 24.7037922512713 24.909470991112777 26.29268691946582 24.094049838150106 38-39 24.729545808570577 24.984323887623066 26.252867903506434 24.033262400299925 40-41 24.71361896520656 24.765928263984733 26.264086382618924 24.256366388189782 42-43 24.604354583044696 24.60220940321427 26.1483890194515 24.645046994289533 44-45 24.499646370453927 24.772286004619794 26.088685603696625 24.639382021229654 46-47 24.591470534459578 24.847211927073605 25.927338949589014 24.63397858887781 48-49 24.506014816007784 25.149095690209666 25.808083624935275 24.536805868847274 50-51 24.65219966898946 25.150068561754257 25.746778587611363 24.450953181644923 52-53 24.779935111673463 25.149523337125252 25.561783013198447 24.508758538002837 54-55 24.508252772596283 24.921419453124276 25.747587798332148 24.82273997594729 56-57 24.546496504606566 25.121620154376163 25.511999316201177 24.819884024816087 58-59 24.463026693226567 25.171279667024404 25.79623593955643 24.569457700192597 60-61 24.73815644049187 25.058998748906408 25.85565924315933 24.34718556744239 62-63 24.659997188232026 25.04998148589397 25.895921022321872 24.394100303552136 64-65 24.507318444751284 24.821890123003733 25.830750404600167 24.84004102764482 66-67 24.449647135234464 25.150789292343113 25.726752244843 24.672811327579424 68-69 24.417667554853864 25.169876527238657 25.723051922979188 24.68940399492829 70-71 24.359768090841648 24.997425871705445 25.919392822538274 24.72341321491463 72-73 24.537167671091282 25.20057965217569 25.629972413387968 24.632280263345052 74-75 24.395600043563924 25.214040785074737 25.682319977029934 24.70803919433141 76-77 24.31309288394062 24.97851558890388 25.63812991237264 25.070261614782858 78-79 24.231448684536634 25.07530157757919 25.748271784654815 24.944977953229362 80-81 24.38550937378719 25.287477327870022 25.481031974633616 24.845981323709175 82-83 24.40432107321877 25.253235326344527 25.58683400726339 24.755609593173304 84-85 24.40992280153341 25.24982574901522 25.45628676431762 24.88396468513375 86-87 24.210006626730276 25.417008783717783 25.47020743513857 24.902777154413375 88-89 24.055399822713174 25.20565205711461 25.586330301328196 25.15261781884402 90-91 24.149978994307506 25.060995712726612 25.68380706972735 25.105218223238534 92-93 24.192812769788446 25.138309893365086 25.706763225079136 24.96211411176733 94-95 24.173290583834287 24.950669212275027 25.67626660242141 25.199773601469282 96-97 24.006109345133115 25.007029443136098 25.678852794880193 25.308008416850598 98-99 23.957852744660475 25.033843343108465 25.60210129043628 25.406202621794783 100-101 23.90276131362387 25.13672581441459 25.717488759639778 25.24302411232176 102-103 23.828405645579405 24.923806723357433 25.88304171150808 25.36474591955508 104-105 23.88804258004219 25.076563815940588 25.724056086955372 25.31133751706185 106-107 23.933183197057822 24.930432165420957 25.690893082026683 25.445491555494538 108-109 23.87996208675954 24.927196545565195 25.708233060820916 25.48460830685435 110-111 23.76435078418612 25.270217824343895 25.615547491409664 25.349883900060327 112-113 23.684120205667064 25.231210439121625 25.721088794577145 25.363580560634162 114-115 23.659407184854093 25.249492434702947 25.690724425705376 25.400375954737587 116-117 23.56184519444586 25.124401917033705 25.733524130242653 25.580228758277784 118-119 23.63421368238393 25.05460241062465 25.681680321944654 25.62950358504676 120-121 23.434748931791415 25.17028467630978 25.811457347727266 25.583509044171542 122-123 23.365939302733885 25.25457737647354 25.67238261190976 25.70710070888282 124-125 23.266038691996197 25.18369151058146 25.6863531462271 25.86391665119524 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 8.5 2 11.5 3 36.5 4 43.5 5 54.5 6 141.0 7 357.5 8 619.5 9 708.5 10 601.5 11 492.0 12 484.0 13 533.5 14 586.0 15 637.5 16 707.0 17 757.0 18 826.0 19 907.5 20 975.0 21 1054.5 22 1179.5 23 1341.0 24 1483.0 25 1716.5 26 2153.0 27 2793.5 28 3535.5 29 4503.0 30 5780.5 31 7251.5 32 9213.5 33 11158.5 34 13119.0 35 15504.0 36 18497.5 37 21642.0 38 24665.5 39 28001.5 40 31407.5 41 34587.0 42 38203.5 43 43061.0 44 47869.0 45 52057.0 46 55576.0 47 59710.0 48 64933.0 49 68476.5 50 69748.0 51 70432.5 52 70181.0 53 69462.5 54 69109.5 55 66914.0 56 62680.5 57 57487.5 58 52674.5 59 47675.0 60 41032.0 61 34752.0 62 29051.5 63 22569.0 64 16684.0 65 12582.0 66 9643.0 67 7517.0 68 5929.0 69 4629.0 70 3601.0 71 2708.0 72 2073.5 73 1708.5 74 1350.0 75 1053.0 76 772.5 77 462.0 78 259.0 79 157.0 80 87.5 81 53.5 82 31.0 83 15.0 84 6.0 85 3.5 86 4.0 87 3.0 88 3.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023893190836631297 2 0.001848092108910708 3 0.03293564151237298 4 0.16547024703711233 5 0.02362917767821548 6 0.06626730276236968 7 0.050690526415836566 8 0.04171407902969884 9 0.0324736184851453 10-11 0.059765978736380225 12-13 0.05432070734405403 14-15 0.055508766556925195 16-17 0.047489366870044805 18-19 0.035014745134897524 20-21 0.02287013984777001 22-23 0.0026731332289601315 24-25 0.004554226982672817 26-27 0.0035311759938115314 28-29 0.004422220403464908 30-31 0.0022771134913364085 32-33 0.008514424358910047 34-35 0.004125205600247117 36-37 0.006072302643563755 38-39 0.0021121052673265233 40-41 0.0032341611905937393 42-43 0.0033991694146036236 44-45 0.004785238496286655 46-47 0.004554226982672817 48-49 0.0016830838849008233 50-51 0.0012210608576731464 52-53 7.590378304454694E-4 54-55 0.00462023027227677 56-57 6.930345408415155E-4 58-59 6.600328960395385E-4 60-61 9.90049344059308E-5 62-63 9.900493440593077E-4 64-65 0.0 66-67 0.0031681579009897854 68-69 3.3001644801976927E-4 70-71 0.0 72-73 0.001947097043316639 74-75 0.004026200665841186 76-77 0.0015840789504948927 78-79 9.90049344059308E-5 80-81 0.0 82-83 0.005412269747524217 84-85 0.001320065792079077 86-87 0.0 88-89 0.0011220559232672156 90-91 6.930345408415155E-4 92-93 0.0 94-95 0.0032011595457917623 96-97 0.001320065792079077 98-99 4.62023027227677E-4 100-101 0.0 102-103 9.57047699257331E-4 104-105 0.0 106-107 5.280263168316308E-4 108-109 0.0025081250049502467 110-111 1.320065792079077E-4 112-113 3.960197376237232E-4 114-115 0.0 116-117 0.0017820888193067542 118-119 0.0021781085569304776 120-121 0.0026071299393561776 122-123 0.0010560526336632617 124-125 0.10118304296286126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1515076.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.6076553353894 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32551350216302 50.28992063981589 2 10.953256232773647 13.715153820433965 3 3.497139041419523 6.568430272953828 4 1.6601237128644284 4.157458129164924 5 0.8999103180409154 2.817063751233315 6 0.5832586970250443 2.1909875684827367 7 0.3900732832355103 1.7095101570646791 8 0.2974599597084082 1.4898616506802271 9 0.22316573023070724 1.257469480885915 >10 1.1114815495662749 12.090390083764136 >50 0.04486872396158158 1.8782265701714187 >100 0.013220460197156244 1.3549578725591271 >500 2.1151552554236577E-4 0.10090778234385024 >1k 3.172732883135486E-4 0.3796622204459562 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2445 0.16137804308166717 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2016 0.133062631841571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.600328960395387E-5 6.600328960395387E-5 10-11 0.0 0.0 0.0 6.600328960395387E-5 1.3200657920790773E-4 12-13 0.0 0.0 0.0 6.600328960395387E-5 1.3200657920790773E-4 14-15 0.0 0.0 0.0 1.3200657920790773E-4 3.300164480197693E-4 16-17 0.0 0.0 0.0 1.3200657920790773E-4 5.280263168316309E-4 18-19 0.0 0.0 0.0 1.3200657920790773E-4 5.940296064355847E-4 20-21 0.0 0.0 0.0 1.980098688118616E-4 6.600328960395386E-4 22-23 0.0 0.0 0.0 3.960197376237232E-4 7.590378304454694E-4 24-25 0.0 0.0 0.0 7.590378304454694E-4 8.580427648514001E-4 26-27 0.0 0.0 0.0 0.0011220559232672156 8.910444096533771E-4 28-29 0.0 0.0 0.0 0.0036301809282174623 9.24046054455354E-4 30-31 0.0 0.0 0.0 0.012540625024751235 9.24046054455354E-4 32-33 0.0 0.0 0.0 0.03145056749628401 0.0011220559232672156 34-35 0.0 0.0 0.0 0.05511274681930148 0.0011880592128711695 36-37 0.0 0.0 0.0 0.08913744261013969 0.0011880592128711695 38-39 0.0 0.0 0.0 0.1364618012561746 0.0013530674368810542 40-41 0.0 0.0 0.0 0.19401666979082238 0.0013860690816830312 42-43 0.0 0.0 0.0 0.24929442483413372 0.001452072371286985 44-45 0.0 0.0 0.0 0.3154297210172955 0.0015180756608909388 46-47 0.0 0.0 0.0 0.3852612014182787 0.0015510773056929157 48-49 0.0 0.0 0.0 0.4542016374096085 0.0015840789504948927 50-51 0.0 0.0 0.0 0.523604096428166 0.0015840789504948927 52-53 0.0 0.0 0.0 0.5970657577573666 0.0018150904641087312 54-55 0.0 0.0 0.0 0.6892393516892883 0.001914095398514662 56-57 0.0 0.0 0.0 0.7894323453080901 0.001914095398514662 58-59 0.0 0.0 0.0 0.8910114080085751 0.001914095398514662 60-61 0.0 0.0 0.0 0.9895873210320802 0.001914095398514662 62-63 0.0 0.0 0.0 1.0963146403216737 0.001980098688118616 64-65 0.0 0.0 0.0 1.2112593691669593 0.001980098688118616 66-67 0.0 0.0 0.0 1.3396687690914515 0.001980098688118616 68-69 0.0 0.0 0.0 1.4829289091768334 0.001980098688118616 70-71 0.0 3.300164480197693E-5 0.0 1.62810314466073 0.0020791036225245468 72-73 0.0 6.600328960395387E-5 0.0 1.769449189347597 0.002343116780940362 74-75 0.0 6.600328960395387E-5 0.0 1.9264710153154034 0.002376118425742339 76-77 0.0 6.600328960395387E-5 0.0 2.114184370949048 0.002376118425742339 78-79 0.0 6.600328960395387E-5 0.0 2.3170454815468005 0.002376118425742339 80-81 0.0 6.600328960395387E-5 0.0 2.515880391478711 0.002409120070544316 82-83 0.0 6.600328960395387E-5 0.0 2.7265958935393337 0.002442121715346293 84-85 0.0 6.600328960395387E-5 0.0 2.951139084771985 0.002442121715346293 86-87 0.0 6.600328960395387E-5 0.0 3.2028096280318614 0.0025411266497522237 88-89 0.0 6.600328960395387E-5 0.0 3.4856997272744072 0.0027061348737621085 90-91 0.0 6.600328960395387E-5 0.0 3.7774012656790816 0.0027061348737621085 92-93 0.0 6.600328960395387E-5 0.0 4.062733486636974 0.0027061348737621085 94-95 0.0 6.600328960395387E-5 0.0 4.3622894165045185 0.0027061348737621085 96-97 0.0 6.600328960395387E-5 0.0 4.693757936895575 0.0028051398081680393 98-99 0.0 6.600328960395387E-5 0.0 5.068854631714845 0.002838141452970016 100-101 0.0 6.600328960395387E-5 0.0 5.475137880871983 0.002838141452970016 102-103 0.0 6.600328960395387E-5 0.0 5.873962758303874 0.002838141452970016 104-105 0.0 6.600328960395387E-5 0.0 6.282457117662744 0.002871143097771993 106-107 0.0 6.600328960395387E-5 0.0 6.7317415099968585 0.0029041447425739698 108-109 0.0 6.600328960395387E-5 0.0 7.226106149130473 0.0029371463873759467 110-111 0.0 6.600328960395387E-5 0.0 7.756937605770272 0.0030031496769799006 112-113 0.0 6.600328960395387E-5 0.0 8.279089629827151 0.0030361513217818776 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2130 0.0 59.92043 1 GTATCAA 3800 0.0 54.14723 1 TCAACGC 4840 0.0 41.644207 4 CAACGCA 4915 0.0 41.085205 5 ATCAACG 5050 0.0 40.03187 3 TATCAAC 5110 0.0 39.445133 2 AACGCAG 5180 0.0 39.228905 6 GTACATG 7775 0.0 35.132236 1 TACATGG 8000 0.0 33.469864 2 ACATGGG 7985 0.0 32.935272 3 ACGCAGA 6280 0.0 31.881042 7 CATGGGG 7220 0.0 31.550732 4 ATGGGGG 4105 0.0 31.198563 5 CGCAGAG 6705 0.0 29.85728 8 GTGGTAT 1260 0.0 29.820211 1 GCAGAGT 7625 0.0 26.098553 9 TACACCG 285 1.1641532E-10 25.08105 5 TGGTATC 1395 0.0 23.9393 2 AGAGTAC 6780 0.0 23.683119 10-11 CAGAGTA 7120 0.0 22.803228 10-11 >>END_MODULE