FastQCFastQC Report
Mon 15 Aug 2016
SRR2921641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149665
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC2900.057.4602782
CGTAGCG1750.054.4113663
GTATCAA21900.038.8664861
GGTATCA14200.036.4681931
GTACATG57950.033.9983521
TACATGG59950.032.2627832
TCAACGC25250.031.8185274
CGTATAC750.00390648931.7399623
ACATGGG61050.031.4865113
CATGGGG48400.030.8628524
CAACGCA26500.030.5408975
AACGCAG27450.029.6981356
ATCAACG27700.029.0042513
TATCAAC28450.028.6580092
AGCGTAG3350.028.4287991
ATGGGGG30950.027.4956635
GTACGTA1107.7339046E-427.0558171
ACTAGCG1150.001003588425.874973
ACGCAGA33700.023.8330297
TAGCGTC2603.039895E-822.8884015
Produced by FastQC (version 0.11.5)