##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921641_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1149665
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.076421392318633	31.0	30.0	33.0	25.0	34.0
2	29.266074900079587	31.0	30.0	34.0	25.0	34.0
3	29.663319314756908	31.0	31.0	34.0	25.0	34.0
4	33.072675083611315	37.0	35.0	37.0	26.0	37.0
5	33.40257292341682	37.0	35.0	37.0	28.0	37.0
6	33.280293824722854	37.0	35.0	37.0	27.0	37.0
7	33.11283460834243	37.0	35.0	37.0	26.0	37.0
8	33.17842414964359	37.0	35.0	37.0	27.0	37.0
9	34.8208043212588	39.0	37.0	39.0	27.0	39.0
10-11	34.89861829315496	39.0	37.0	39.0	27.0	39.0
12-13	34.857948184906036	39.0	37.0	39.0	27.0	39.0
14-15	36.112878969091	40.0	37.5	41.0	27.0	41.0
16-17	36.080869209726316	40.0	37.5	41.0	27.0	41.0
18-19	36.000159611712974	40.0	37.0	41.0	26.0	41.0
20-21	36.081911252408304	40.0	37.5	41.0	27.0	41.0
22-23	36.005654255804956	40.0	37.0	41.0	26.0	41.0
24-25	36.001935781292815	40.0	37.0	41.0	26.0	41.0
26-27	35.97701939260567	40.0	37.0	41.0	26.5	41.0
28-29	35.944242018327074	40.0	37.0	41.0	26.0	41.0
30-31	35.84585814128464	40.0	37.0	41.0	26.0	41.0
32-33	35.766294529275925	40.0	37.0	41.0	25.0	41.0
34-35	35.63499410697899	40.0	36.5	41.0	25.0	41.0
36-37	35.51918906811984	40.0	36.0	41.0	24.0	41.0
38-39	35.386614796484196	40.0	36.0	41.0	24.0	41.0
40-41	35.27502272401091	40.0	35.5	41.0	23.5	41.0
42-43	35.004653094597124	40.0	35.0	41.0	22.5	41.0
44-45	34.61790347623003	39.5	35.0	41.0	20.5	41.0
46-47	34.35026899140185	39.0	34.5	41.0	18.5	41.0
48-49	34.1479765844833	39.0	34.0	40.5	17.0	41.0
50-51	33.941911339390174	38.5	34.0	40.0	15.0	41.0
52-53	33.734044699977815	38.0	34.0	40.0	13.5	41.0
54-55	33.49553348149243	38.0	33.0	40.0	10.5	41.0
56-57	33.24274158124323	38.0	33.0	40.0	8.5	41.0
58-59	33.259868744373364	37.5	33.0	40.0	8.0	41.0
60-61	33.047537760999944	37.0	33.0	40.0	7.5	41.0
62-63	32.7387552026025	36.5	33.0	40.0	4.5	41.0
64-65	32.44597078279325	36.0	33.0	39.0	2.0	41.0
66-67	32.12603541031518	35.5	32.0	39.0	2.0	41.0
68-69	31.79839040068194	35.0	32.0	39.0	2.0	40.0
70-71	31.445140106030887	35.0	32.0	37.5	2.0	40.0
72-73	31.105409401869238	35.0	31.5	37.0	2.0	39.0
74-75	30.7444751297117	35.0	31.0	36.5	2.0	39.0
76-77	30.445316244297253	35.0	31.0	36.0	2.0	39.0
78-79	30.180564773216545	35.0	31.0	36.0	2.0	37.0
80-81	29.92454236668943	35.0	31.0	35.0	2.0	37.0
82-83	29.710222977997937	35.0	31.0	35.0	2.0	36.5
84-85	29.49673209152231	34.0	30.0	35.0	2.0	36.0
86-87	29.330379284400237	34.0	30.0	35.0	2.0	36.0
88-89	29.169614626869567	34.0	30.0	35.0	2.0	35.5
90-91	28.988039559349897	34.0	30.0	35.0	2.0	35.0
92-93	28.913748352781028	34.0	30.0	35.0	2.0	35.0
94-95	28.792907934050355	34.0	29.5	35.0	2.0	35.0
96-97	28.706466666376727	34.0	29.0	35.0	2.0	35.0
98-99	28.624334914953486	34.0	29.0	35.0	2.0	35.0
100-101	28.525346948893805	34.0	29.0	35.0	2.0	35.0
102-103	27.639088778035344	32.5	28.0	34.5	2.0	35.0
104-105	28.57756346413955	34.0	29.5	35.0	2.0	35.0
106-107	28.869254521969445	34.0	30.0	35.0	2.0	35.0
108-109	28.879862829606886	34.0	30.0	35.0	2.0	35.0
110-111	28.830512366645937	34.0	30.0	35.0	2.0	35.0
112-113	28.761484867330918	34.0	30.0	35.0	2.0	35.0
114-115	28.65226522508731	34.0	30.0	35.0	2.0	35.0
116-117	28.54417373756703	34.0	30.0	35.0	2.0	35.0
118-119	28.268357304084233	34.0	29.5	35.0	2.0	35.0
120-121	28.15844050223326	34.0	29.0	35.0	2.0	35.0
122-123	27.91672443711864	34.0	29.0	35.0	2.0	35.0
124-125	27.129930023093685	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57455.0
3	8835.0
4	4516.0
5	3899.0
6	4025.0
7	4365.0
8	4409.0
9	4666.0
10	5059.0
11	5199.0
12	5221.0
13	5194.0
14	5323.0
15	4853.0
16	4283.0
17	3822.0
18	3552.0
19	3231.0
20	3246.0
21	3366.0
22	3796.0
23	5154.0
24	7543.0
25	9461.0
26	10383.0
27	11421.0
28	13701.0
29	16468.0
30	20001.0
31	25242.0
32	33261.0
33	46571.0
34	72096.0
35	117993.0
36	225346.0
37	310969.0
38	75679.0
39	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03242174279592	17.874459601368734	12.073434181643544	25.019684474191806
2	17.090880937281703	18.945801657536904	38.517855449628854	25.44546195555254
3	20.33606076791521	22.896358065823787	27.545726429554417	29.221854736706582
4	14.198611273011338	14.651835252648446	34.01772184052431	37.13183163381591
5	14.83508453255247	37.359699625401696	32.934463565834825	14.870752276211007
6	35.143723240698336	34.28446563230739	16.019016842145582	14.552794284848686
7	31.488587320250407	31.40747535527824	18.635996132386563	18.467941192084794
8	27.622245872779533	35.17990425875373	18.0475991155117	19.15025075295504
9	27.699049753520576	16.37430767383629	17.853793242653033	38.0728493299901
10-11	26.984613898530313	25.544861879270382	25.784479709064705	21.6860445131346
12-13	26.53871231104687	22.850230682338655	26.258422504261546	24.35263450235293
14-15	24.066549790536516	24.263658628895087	23.21695022903951	28.45284135152889
16-17	24.82211867050265	27.34728308701491	23.983776091618182	23.84682215086425
18-19	24.810862243670428	26.628298897912817	25.30119119043776	23.259647667978996
20-21	25.310937427948215	25.512747673244025	25.34399967981819	23.832315218989567
22-23	25.36230055523022	24.925712615063873	25.198106944249876	24.51387988545603
24-25	24.95314645279059	25.44539147291543	25.110412537434986	24.491049536858995
26-27	24.612968881749605	25.639511763431333	25.288310011251337	24.459209343567725
28-29	24.670069666945707	25.990052054417838	25.178387757552763	24.161490521083692
30-31	25.184956722715214	25.51591473915958	25.20614186388351	24.092986674241697
32-33	24.584493590075436	25.650008417797704	25.32421572009583	24.441282272031028
34-35	24.65897541211851	25.901639772220985	25.325491827244374	24.113892988416126
36-37	24.859110424284548	25.80650773484532	25.188059366519933	24.146322474350203
38-39	24.897112807688277	25.924179501556633	25.18333951751225	23.99536817324285
40-41	24.76283831760087	25.79780476429148	25.24899583716203	24.190361080945618
42-43	24.715900687475965	25.582553666103486	25.21158460513197	24.489961041288577
44-45	24.52385364279258	25.64443852877235	25.45913879993632	24.37256902849875
46-47	24.51917660257428	25.560364309120736	25.391277339605544	24.52918174869944
48-49	24.45302708447438	25.88227156515191	25.341617446753528	24.323083903620184
50-51	24.404687846661822	25.85763319426983	25.381888102457467	24.35579085661088
52-53	24.59608432018493	25.785830836750684	25.31389080171515	24.304194041349238
54-55	24.4035633298403	25.514722290734408	25.46617651088952	24.615537868535768
56-57	24.438264443432328	25.717141846683482	25.164247809630446	24.680345900253737
58-59	24.499579376119527	25.78232996921225	25.3342154690413	24.38387518562693
60-61	24.74925102650803	25.734803135357247	25.423903553703585	24.09204228443114
62-63	24.535924978924463	25.828688305666166	25.439976266641846	24.195410448767525
64-65	24.430387400511638	25.431045545083336	25.5545400883123	24.584026966092722
66-67	24.51046328713919	25.744767486374933	25.297732983455024	24.447036243030855
68-69	24.432968209853854	25.70916764287644	25.383092554256308	24.474771593013397
70-71	24.3605033391622	25.566291365342934	25.512991818845936	24.560213476648926
72-73	24.52551461398894	25.85948072500314	25.196369155307046	24.418635505700873
74-75	24.398262824931987	25.880472438547542	25.309170622864684	24.412094113655787
76-77	24.33654243905664	25.55290848676739	25.425939079913302	24.684609994262672
78-79	24.38335057425763	25.582327081069465	25.47688048602876	24.55744185864414
80-81	24.424520000156598	25.86285569620694	25.220245969062688	24.49237833457378
82-83	24.498053987227266	25.88412317192904	25.27571958101673	24.34210325982696
84-85	24.50686839355882	25.93169795596677	25.126028562704594	24.435405087769816
86-87	24.26427730055799	25.996361839506836	25.29635087804768	24.443009981887492
88-89	24.2289746661551	25.707427543324364	25.336150428349825	24.72744736217071
90-91	24.50226351617347	25.625778375949437	25.341854669516202	24.53010343836089
92-93	24.476764517066265	25.793552068672156	25.426243369305958	24.303440044955625
94-95	24.398990731554342	25.64626628524056	25.388686224251238	24.566056758953863
96-97	24.219629117491365	25.685351432773253	25.401576248486847	24.69344320124854
98-99	24.18456833107093	25.65352619041302	25.319711671242935	24.842193807273112
100-101	24.16434149661935	25.80162366823756	25.38050510740002	24.653529727743067
102-103	24.0246621802352	25.718534343469052	25.60362625906337	24.653177217232386
104-105	24.059284117790586	25.797907389116283	25.445659487315986	24.697149005777145
106-107	24.01254286602749	25.705133920319746	25.56243924821088	24.719883965441884
108-109	24.097236086807307	25.667294324589186	25.549809636528213	24.685659952075294
110-111	23.99233155583042	26.049920562538137	25.41202882799191	24.54571905363953
112-113	23.896553388832036	26.18771237691218	25.41185844395163	24.503875790304154
114-115	23.91488451054157	26.137115432043185	25.440955598443004	24.507044458972242
116-117	23.77936016116882	26.030748032797433	25.501956329233945	24.687935476799797
118-119	23.852815306524295	25.886061955103894	25.45705746619142	24.804065272180388
120-121	23.709522533775438	26.04401157915613	25.60457425377419	24.64189163329425
122-123	23.743686138339136	26.068126643430674	25.520526628469636	24.667660589760562
124-125	23.65183369987117	26.027400248411002	25.468628185792586	24.852137865925243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	385.0
2	236.0
3	168.0
4	136.5
5	121.0
6	119.5
7	148.5
8	181.5
9	182.5
10	184.5
11	193.5
12	197.0
13	212.0
14	234.0
15	267.0
16	294.0
17	318.0
18	357.5
19	431.5
20	492.0
21	557.5
22	693.0
23	876.0
24	1106.5
25	1423.0
26	1897.5
27	2553.0
28	3223.0
29	4050.0
30	5344.5
31	6860.0
32	8804.0
33	10739.5
34	12469.5
35	14418.0
36	16811.5
37	19247.0
38	21151.0
39	23359.0
40	25550.5
41	27317.5
42	29418.5
43	32815.0
44	36343.5
45	39444.5
46	41753.5
47	43396.5
48	46290.0
49	49039.0
50	50505.5
51	51194.5
52	50708.5
53	50216.5
54	50291.0
55	48955.0
56	46176.0
57	42483.0
58	39153.0
59	35878.5
60	30679.0
61	25596.0
62	21385.0
63	16740.0
64	12651.0
65	9767.5
66	7505.0
67	5842.0
68	4711.5
69	3746.5
70	2974.5
71	2363.5
72	1836.0
73	1499.5
74	1221.5
75	942.0
76	710.5
77	497.0
78	344.5
79	251.0
80	176.0
81	121.5
82	96.5
83	76.5
84	61.0
85	41.5
86	29.5
87	33.5
88	36.5
89	29.5
90	15.5
91	8.5
92	10.0
93	8.0
94	7.5
95	6.5
96	3.5
97	3.0
98	5.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024528884501137285
2	0.011133678071438202
3	0.009654986452575315
4	0.010698768771772647
5	0.01452597060882953
6	0.08689487807317785
7	0.05532046291745857
8	0.04575245832481636
9	0.024267938921337957
10-11	0.0472746408736458
12-13	0.012220951320602089
14-15	0.026094557979933285
16-17	0.032487724685016935
18-19	0.03796758186080293
20-21	0.027660231458729284
22-23	0.03583652629244171
24-25	0.029486850517324612
26-27	0.04070751044869592
28-29	0.04201223834769259
30-31	0.023876520551638955
32-33	0.027355794948963397
34-35	0.04183827462782636
36-37	0.008219785763678985
38-39	0.01970139127484963
40-41	0.03905485510996682
42-43	0.02113659196374596
44-45	0.015308807348227526
46-47	0.02231084707284296
48-49	0.027529758668829618
50-51	0.026964376579264395
52-53	0.022658774512575402
54-55	0.020397246154314517
56-57	0.02248481079270918
58-59	0.01583069850782619
60-61	0.02248481079270918
62-63	0.019831864064749297
64-65	0.019266481975184074
66-67	0.027225322159063724
68-69	0.020571209874180738
70-71	0.043447439036588924
72-73	0.020484228014247628
74-75	0.008480731343478317
76-77	0.015265316418260972
78-79	0.023311138462073736
80-81	0.018396663375852967
82-83	0.022919720092374737
84-85	0.010785750631705759
86-87	0.015917680367759302
88-89	0.02209339242301018
90-91	0.020571209874180738
92-93	0.007567421814180653
94-95	0.01039433226200676
96-97	0.01422153409906364
98-99	0.006480148565016766
100-101	0.009350549942809426
102-103	0.004392583926622103
104-105	0.004218620206755881
106-107	6.523639494983322E-4
108-109	0.013351715499732532
110-111	0.0016091644087625526
112-113	0.002478983008093662
114-115	0.012568878760334534
116-117	0.005479857175785989
118-119	0.2760369324977276
120-121	0.0011307641791304425
122-123	0.0010437823191973314
124-125	0.31143854949050376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1149665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.86842509636088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.82513994654299	56.89080231653922
2	9.251907751025279	12.558248163977998
3	2.7954057510566472	5.691593574885736
4	1.283431135141431	3.484177994467345
5	0.7261919804330347	2.4642753014798693
6	0.46927217960712364	1.910925826288325
7	0.32356393275649475	1.5371842173917682
8	0.2248051714740418	1.2205738353168456
9	0.17528612671031119	1.0706754024962981
>10	0.8781002909910881	10.3567147944723
>50	0.036404292156541125	1.655425038019024
>100	0.010363498405278003	1.1022671745950388
>500	1.279436996565245E-4	0.05713636007022104
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.698185993311095E-5	0.0	0.0	0.0
6	0.0	8.698185993311095E-5	0.0	0.0	0.0
7	0.0	8.698185993311095E-5	0.0	8.698185993311095E-5	0.0
8	0.0	8.698185993311095E-5	0.0	8.698185993311095E-5	0.0
9	0.0	8.698185993311095E-5	0.0	8.698185993311095E-5	0.0
10-11	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	0.0
12-13	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	0.0
14-15	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	0.0
16-17	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	8.698185993311095E-5
18-19	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	8.698185993311095E-5
20-21	0.0	8.698185993311095E-5	0.0	1.739637198662219E-4	8.698185993311095E-5
22-23	0.0	8.698185993311095E-5	0.0	3.044365097658883E-4	1.739637198662219E-4
24-25	0.0	8.698185993311095E-5	0.0	4.349092996655548E-4	2.6094557979933284E-4
26-27	0.0	8.698185993311095E-5	0.0	5.653820895652212E-4	2.6094557979933284E-4
28-29	0.0	8.698185993311095E-5	0.0	0.0026964376579264398	3.479274397324438E-4
30-31	0.0	8.698185993311095E-5	0.0	0.010959714351571979	4.349092996655548E-4
32-33	0.0	8.698185993311095E-5	0.0	0.021919428703143957	5.218911595986657E-4
34-35	0.0	8.698185993311095E-5	0.0	0.03657587210187316	5.218911595986657E-4
36-37	0.0	8.698185993311095E-5	0.0	0.05893021010468267	5.218911595986657E-4
38-39	0.0	8.698185993311095E-5	0.0	0.09811553800454914	5.218911595986657E-4
40-41	0.0	8.698185993311095E-5	0.0	0.14786916188628862	5.218911595986657E-4
42-43	0.0	8.698185993311095E-5	0.0	0.1924038741720414	5.218911595986657E-4
44-45	0.0	8.698185993311095E-5	0.0	0.23646018622816212	5.218911595986657E-4
46-47	0.0	8.698185993311095E-5	0.0	0.2902149756668247	5.218911595986657E-4
48-49	0.0	8.698185993311095E-5	0.0	0.34688365741324645	5.218911595986657E-4
50-51	0.0	8.698185993311095E-5	0.0	0.4058573584478957	5.218911595986657E-4
52-53	0.0	8.698185993311095E-5	0.0	0.4659618236616754	5.218911595986657E-4
54-55	0.0	1.3047278989966642E-4	0.0	0.5266751618949868	5.653820895652211E-4
56-57	0.0	1.739637198662219E-4	0.0	0.5944340307828803	6.088730195317766E-4
58-59	0.0	1.739637198662219E-4	0.0	0.6757185788903723	6.088730195317766E-4
60-61	0.0	1.739637198662219E-4	0.0	0.7573945453675636	6.088730195317766E-4
62-63	0.0	1.739637198662219E-4	0.0	0.8452897148299722	6.088730195317766E-4
64-65	0.0	1.739637198662219E-4	0.0	0.931401756163752	7.828367393979985E-4
66-67	0.0	1.739637198662219E-4	0.0	1.0268643474403414	8.26327669364554E-4
68-69	0.0	1.739637198662219E-4	0.0	1.1318079614496397	8.698185993311095E-4
70-71	0.0	1.739637198662219E-4	0.0	1.2507991458381356	8.698185993311095E-4
72-73	0.0	1.739637198662219E-4	0.0	1.3607007258636212	8.698185993311095E-4
74-75	0.0	1.739637198662219E-4	0.0	1.4771259453840901	8.698185993311095E-4
76-77	0.0	1.739637198662219E-4	0.0	1.6143833203585392	8.698185993311095E-4
78-79	0.0	1.739637198662219E-4	0.0	1.77042877707854	8.698185993311095E-4
80-81	0.0	1.739637198662219E-4	0.0	1.9202115398833572	8.698185993311095E-4
82-83	0.0	1.739637198662219E-4	0.0	2.0831285635380743	8.698185993311095E-4
84-85	0.0	1.739637198662219E-4	0.0	2.251786389948376	8.698185993311095E-4
86-87	0.0	1.739637198662219E-4	0.0	2.432099785589715	9.13309529297665E-4
88-89	0.0	1.739637198662219E-4	0.0	2.6408997403591483	9.568004592642205E-4
90-91	0.0	1.739637198662219E-4	0.0	2.8607029004101197	0.0010437823191973314
92-93	0.0	1.739637198662219E-4	0.0	3.0823761704496526	0.0010437823191973314
94-95	0.0	1.739637198662219E-4	0.0	3.308746460925574	0.0010437823191973314
96-97	0.0	1.739637198662219E-4	0.0	3.5628204737901914	0.0012177460390635532
98-99	0.0	1.739637198662219E-4	0.0	3.838509478848186	0.0013917097589297752
100-101	0.0	1.739637198662219E-4	0.0	4.149208682529259	0.0013917097589297752
102-103	0.0	3.479274397324438E-4	0.0	4.46395254269722	0.0013917097589297752
104-105	0.0	3.479274397324438E-4	0.0	4.797136557171002	0.0013917097589297752
106-107	0.0	3.479274397324438E-4	0.0	5.14449861481388	0.0013917097589297752
108-109	0.0	3.479274397324438E-4	0.0	5.518390139736358	0.0013917097589297752
110-111	0.0	3.479274397324438E-4	0.0	5.922159933545859	0.0013917097589297752
112-113	0.0	3.479274397324438E-4	0.0	6.334366967768872	0.0013917097589297752
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGC	290	0.0	57.460278	2
CGTAGCG	175	0.0	54.411366	3
GTATCAA	2190	0.0	38.866486	1
GGTATCA	1420	0.0	36.468193	1
GTACATG	5795	0.0	33.998352	1
TACATGG	5995	0.0	32.262783	2
TCAACGC	2525	0.0	31.818527	4
CGTATAC	75	0.003906489	31.739962	3
ACATGGG	6105	0.0	31.486511	3
CATGGGG	4840	0.0	30.862852	4
CAACGCA	2650	0.0	30.540897	5
AACGCAG	2745	0.0	29.698135	6
ATCAACG	2770	0.0	29.004251	3
TATCAAC	2845	0.0	28.658009	2
AGCGTAG	335	0.0	28.428799	1
ATGGGGG	3095	0.0	27.495663	5
GTACGTA	110	7.7339046E-4	27.055817	1
ACTAGCG	115	0.0010035884	25.87497	3
ACGCAGA	3370	0.0	23.833029	7
TAGCGTC	260	3.039895E-8	22.888401	5
>>END_MODULE