FastQCFastQC Report
Mon 15 Aug 2016
SRR2921641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149665
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.2017979150448174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.1620472050553857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.11020601653525157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33800.047.4629171
GGTATCA20850.045.4788971
TCAACGC40650.038.7211844
CAACGCA42500.037.141965
AACGCAG43950.036.4698266
ATCAACG43300.036.2184563
GTACATG60300.036.0000341
TATCAAC44650.035.524032
TACATGG62950.034.385312
ACATGGG62500.034.060443
CATGGGG51750.033.296054
ATGGGGG31900.032.1177865
ACGCAGA53750.029.598737
CGCAGAG55600.028.612638
GTATAGG3650.027.7763331
GCAGAGT60850.025.9481649
TGGGGGG21400.023.9455576
GAGTACT28850.022.59937912-13
AGAGTAC55850.022.51026710-11
TACCGTT1602.586026E-422.3445457
Produced by FastQC (version 0.11.5)