##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921641_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149665 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756081119282573 33.0 31.0 34.0 30.0 34.0 2 31.93944235929597 33.0 31.0 34.0 30.0 34.0 3 31.997078279324846 34.0 31.0 34.0 30.0 34.0 4 35.39478108840402 37.0 35.0 37.0 33.0 37.0 5 35.26575828610943 37.0 35.0 37.0 33.0 37.0 6 35.29241909599753 37.0 35.0 37.0 33.0 37.0 7 35.18876281351524 37.0 35.0 37.0 33.0 37.0 8 35.2256231162991 37.0 36.0 37.0 33.0 37.0 9 36.94469345418013 39.0 38.0 39.0 34.0 39.0 10-11 36.891588854144466 39.0 38.0 39.0 34.0 39.0 12-13 36.827708071481695 39.0 38.0 39.0 33.5 39.0 14-15 38.25402269356725 41.0 39.0 41.0 34.0 41.0 16-17 38.25567534890598 41.0 39.0 41.0 34.0 41.0 18-19 38.2706088295286 41.0 39.0 41.0 34.0 41.0 20-21 38.21504177303824 41.0 39.0 41.0 34.0 41.0 22-23 38.21578285848486 41.0 39.0 41.0 34.0 41.0 24-25 38.176844559067206 41.0 39.0 41.0 34.0 41.0 26-27 38.09900927661536 41.0 39.0 41.0 34.0 41.0 28-29 38.03145220564251 41.0 39.0 41.0 33.5 41.0 30-31 37.915003935929164 41.0 38.0 41.0 33.0 41.0 32-33 37.788722366950374 41.0 38.0 41.0 33.0 41.0 34-35 37.65670608394619 41.0 38.0 41.0 33.0 41.0 36-37 37.58135848268844 40.0 38.0 41.0 33.0 41.0 38-39 37.49854609821122 40.0 38.0 41.0 33.0 41.0 40-41 37.38132673430956 40.0 38.0 41.0 32.0 41.0 42-43 37.21666441963528 40.0 37.5 41.0 31.5 41.0 44-45 37.044905689918366 40.0 37.0 41.0 31.0 41.0 46-47 36.82044073708428 40.0 37.0 41.0 31.0 41.0 48-49 36.611750379458364 40.0 37.0 41.0 30.0 41.0 50-51 36.36312317066276 40.0 36.0 41.0 30.0 41.0 52-53 36.235485989396906 40.0 36.0 41.0 30.0 41.0 54-55 36.10738736936412 40.0 35.0 41.0 29.0 41.0 56-57 35.91282243088204 39.0 35.0 41.0 29.0 41.0 58-59 35.71351828576151 39.0 35.0 41.0 29.0 41.0 60-61 35.45439628065567 39.0 35.0 41.0 29.0 41.0 62-63 35.10352537478309 38.0 35.0 41.0 28.0 41.0 64-65 34.81467210013352 37.0 35.0 40.0 28.0 41.0 66-67 34.50863381941696 37.0 35.0 39.5 28.0 41.0 68-69 34.13853035449458 36.0 35.0 39.0 28.0 41.0 70-71 33.76685121317949 35.5 34.5 39.0 27.0 41.0 72-73 33.44649484849934 35.0 34.0 37.5 26.5 40.0 74-75 33.12768806565391 35.0 34.0 37.0 26.0 39.0 76-77 32.80551682446625 35.0 34.0 36.5 26.0 39.0 78-79 32.548815959431664 35.0 34.0 36.0 26.0 38.5 80-81 32.30125340860164 35.0 34.0 36.0 26.0 37.0 82-83 32.077345139671124 35.0 34.0 35.0 25.5 37.0 84-85 31.86443181274545 35.0 34.0 35.0 25.0 36.5 86-87 31.735364649702305 35.0 34.0 35.0 25.0 36.0 88-89 31.63517720379415 35.0 34.0 35.0 25.0 36.0 90-91 31.525850138953523 35.0 33.5 35.0 24.5 36.0 92-93 31.44706284004471 35.0 33.0 35.0 24.0 35.0 94-95 31.377242501076402 35.0 33.0 35.0 24.0 35.0 96-97 31.31438549490504 35.0 33.0 35.0 24.0 35.0 98-99 31.239017452910197 35.0 33.0 35.0 23.0 35.0 100-101 31.17391457511536 35.0 33.0 35.0 23.0 35.0 102-103 30.48422366515463 34.0 31.5 35.0 20.0 35.0 104-105 30.99871223356369 35.0 33.0 35.0 21.0 35.0 106-107 31.10225065562577 35.0 33.0 35.0 23.0 35.0 108-109 31.090903002178894 35.0 33.0 35.0 21.5 35.0 110-111 31.02752932375953 35.0 33.0 35.0 20.0 35.0 112-113 30.990982155671436 35.0 33.0 35.0 20.0 35.0 114-115 30.868249011668617 35.0 33.0 35.0 19.0 35.0 116-117 31.05621420152827 35.0 33.0 35.0 20.0 35.0 118-119 31.045275362823084 35.0 33.0 35.0 19.5 35.0 120-121 30.97430773312226 35.0 33.0 35.0 18.5 35.0 122-123 30.845809431443072 35.0 33.0 35.0 8.5 35.0 124-125 30.201844450339884 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17989.0 3 4964.0 4 2637.0 5 2185.0 6 2103.0 7 2385.0 8 2689.0 9 2909.0 10 3021.0 11 3393.0 12 3866.0 13 5153.0 14 7264.0 15 6545.0 16 3739.0 17 3277.0 18 2854.0 19 2519.0 20 2367.0 21 2437.0 22 2673.0 23 3281.0 24 4439.0 25 5669.0 26 6755.0 27 7991.0 28 9436.0 29 11720.0 30 13984.0 31 17408.0 32 22930.0 33 31596.0 34 49037.0 35 87771.0 36 203378.0 37 429020.0 38 157977.0 39 304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.493225172742 18.050864710519036 11.36564926805417 27.090260848684792 2 15.897585331060156 19.3998121150969 37.32907692842977 27.373525625413176 3 19.118551691314725 22.940661073180237 28.075989743438036 29.864797492067 4 13.23452664008162 14.618087532007914 33.9289557164676 38.21843011144287 5 14.601003461742913 37.316962192268974 32.62868870500887 15.45334564097924 6 35.50247496960144 33.7338313156115 15.786015007359195 14.977678707427868 7 30.993601904808315 31.44604609552586 18.55995864560888 19.000393354056943 8 28.578638637575953 34.77876098493979 17.725922761047595 18.916677616436665 9 27.922951965050302 15.254905338773122 18.003426406649563 38.81871628952701 10-11 27.27285386606765 25.01530691507023 25.65943756715134 22.052401651710785 12-13 27.038807575623075 22.210923072370836 26.488746394860467 24.26152295714562 14-15 24.208829707195083 23.57865229410774 23.83891269375388 28.373605304943293 16-17 24.45578937246246 26.836005163958287 24.53428432315727 24.17392114042198 18-19 24.399988166390983 25.77434003701483 26.588509739669302 23.23716205692488 20-21 24.931997395172893 24.859612086084574 26.65010446713929 23.558286051603243 22-23 24.700611107800878 24.29800453796797 26.63440131137445 24.366983042856702 24-25 24.37376969895191 24.70901178392456 26.542970397967323 24.374248119156206 26-27 24.12885166731615 24.92836838223014 26.733250292267442 24.20952965818627 28-29 24.13823398421734 25.347768298283953 26.544863676218846 23.969134041279872 30-31 24.693350057387455 24.91298374319299 26.511177614934745 23.882488584484815 32-33 24.307662329810697 24.85513004696122 26.608749131737163 24.228458491490915 34-35 24.419783005237413 25.059433043989586 26.585729284550542 23.93505466622246 36-37 24.401312458789942 25.003479477173762 26.657231484397155 23.937976579639145 38-39 24.39672419192095 25.16600848961414 26.57797223478654 23.85929508367837 40-41 24.384541113227733 25.011438462809778 26.5572401555283 24.046780268434194 42-43 24.300174274144055 24.940861176096043 26.435521828630282 24.32344272112962 44-45 24.180954414758673 25.065163091485804 26.477591855711758 24.27629063804376 46-47 24.295895325047667 25.053017703302665 26.214405505838474 24.43668146581119 48-49 24.241015374752802 25.38158535094979 26.03926692120912 24.338132353088284 50-51 24.24432337362246 25.449474339330674 26.020953360962068 24.285248926084797 52-53 24.495381442527783 25.3726442399484 25.85417979559964 24.277794521924175 54-55 24.22108231675493 25.128043646182004 26.09519940710225 24.555674629960823 56-57 24.240534350680246 25.349952203135366 25.804740022458873 24.60477342372551 58-59 24.23936008739998 25.404620682516608 26.001581341217424 24.354437888865984 60-61 24.565470301066618 25.214356387200592 26.104095767236036 24.116077544496754 62-63 24.33818045017951 25.427205177216592 26.083403463220407 24.15121090938349 64-65 24.268939212727187 24.99084515924204 26.15192251655917 24.5882931114716 66-67 24.20729137535626 25.43052325619325 25.928123112145464 24.434062256305026 68-69 24.193881585041847 25.327868531623032 25.959011377706727 24.5192385056284 70-71 24.09628022075996 25.154023128476556 26.166013577868334 24.58368307289515 72-73 24.33066003270589 25.472756689050485 25.76019449566821 24.436388782575417 74-75 24.109627410506064 25.58535424837966 25.86284134609948 24.4421769950148 76-77 24.127479518650784 25.175978466368054 25.914816201718182 24.78172581326298 78-79 24.05507009886384 25.204277431105837 26.00729605744993 24.73335641258039 80-81 24.167561854975144 25.506908534225186 25.658517916088602 24.667011694711068 82-83 24.232129560685035 25.549752611324905 25.758954356615472 24.45916347137459 84-85 24.20028608818979 25.506384359352513 25.620854428604297 24.672475123853395 86-87 23.9365380349928 25.620811279807597 25.78207564812358 24.66057503707602 88-89 23.900110424959955 25.336156802573306 25.80147253575981 24.962260236706925 90-91 24.07753968250517 25.262502321345547 25.895734943437454 24.76422305271183 92-93 24.084102760369326 25.466418478426323 25.862011977402112 24.58746678380224 94-95 24.034823256793032 25.22768115765539 25.83770139018157 24.899794195370003 96-97 23.804687958700384 25.253392887819963 25.920162484587667 25.021756668891985 98-99 23.80609007465267 25.247910790822488 25.734273033490407 25.211726101034436 100-101 23.767488790212802 25.316070333531943 25.872971691753683 25.04346918450157 102-103 23.692801219150475 25.24553060793126 25.94448063894062 25.117187533977642 104-105 23.772881665528654 25.28375657256679 25.840570948928598 25.102790812975957 106-107 23.73140533967493 25.25664154035374 25.860429797383222 25.15152332258811 108-109 23.795883928882088 25.08372186075641 25.893279635364113 25.22711457499739 110-111 23.68698479467461 25.41179458676151 25.788731132514485 25.112489486049387 112-113 23.627420280778665 25.483447280065413 25.779839256823756 25.109293182332166 114-115 23.556949198244705 25.535873493582912 25.761852365689137 25.145324942483242 116-117 23.383184975877324 25.343269158422448 25.861472829209376 25.412073036490845 118-119 23.450742602354914 25.201856755965675 25.86554306806612 25.481857573613294 120-121 23.32107728011301 25.271172788604396 25.98692107762805 25.420828853654548 122-123 23.250611163469973 25.330893753454852 25.894499333058757 25.52399575001642 124-125 23.181047817109363 25.327695891595337 25.731653535902776 25.759602755392525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37.0 1 28.5 2 22.5 3 35.0 4 41.5 5 48.5 6 126.5 7 348.0 8 611.0 9 718.5 10 634.0 11 491.5 12 469.0 13 538.5 14 600.5 15 650.5 16 700.0 17 712.5 18 780.0 19 839.5 20 863.5 21 943.0 22 1059.0 23 1241.0 24 1412.5 25 1675.0 26 2084.0 27 2648.0 28 3257.5 29 4158.0 30 5410.0 31 6618.5 32 8492.0 33 10428.0 34 12007.0 35 13988.5 36 16403.0 37 18708.5 38 20667.5 39 23065.5 40 25419.0 41 27093.5 42 28978.5 43 32272.0 44 35791.0 45 39024.0 46 41344.0 47 43532.0 48 46909.5 49 49364.5 50 50928.5 51 51587.0 52 51262.0 53 51005.5 54 50933.0 55 49661.5 56 46798.5 57 43002.5 58 39567.5 59 35913.5 60 30316.0 61 25246.0 62 21196.5 63 16490.5 64 12206.0 65 9213.0 66 7040.5 67 5429.0 68 4268.0 69 3311.5 70 2570.0 71 2016.0 72 1616.5 73 1399.5 74 1088.5 75 800.0 76 567.5 77 355.0 78 221.0 79 133.0 80 95.5 81 67.5 82 34.0 83 16.0 84 12.0 85 8.5 86 7.0 87 4.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02287622916240818 2 0.002174546498327774 3 0.03061761469645505 4 0.165439497592777 5 0.021223573823679072 6 0.06628017726903054 7 0.049840605741672574 8 0.04157732904802704 9 0.030965542136187497 10-11 0.058669264524883344 12-13 0.05314591641913079 14-15 0.054233189668294685 16-17 0.04753558645344513 18-19 0.03444481653351194 20-21 0.022658774512575402 22-23 0.0027399285878929947 24-25 0.0041751292767893255 26-27 0.002870401377792661 28-29 0.004262111136722436 30-31 0.0020440737084281074 32-33 0.00865469506334454 34-35 0.00452305671652177 36-37 0.005827784615518434 38-39 0.002174546498327774 40-41 0.0030443650976588834 42-43 0.0034357834673578824 44-45 0.004305602066688992 46-47 0.004262111136722436 48-49 0.0017831281286287744 50-51 0.0014352006888963306 52-53 8.26327669364554E-4 54-55 0.004957966016187324 56-57 6.958548794648876E-4 58-59 6.958548794648876E-4 60-61 2.174546498327774E-4 62-63 0.001087273249163887 64-65 0.0 66-67 0.0040446564868896594 68-69 3.9141836969899926E-4 70-71 0.0 72-73 0.002174546498327774 74-75 0.0053928753158528795 76-77 0.0018701099885618856 78-79 2.6094557979933284E-4 80-81 0.0 82-83 0.004957966016187324 84-85 0.0016961462686956635 86-87 0.0 88-89 0.0013482188289632199 90-91 7.393458094314431E-4 92-93 0.0 94-95 0.0026964376579264398 96-97 0.0015221825488294417 98-99 6.523639494983322E-4 100-101 0.0 102-103 0.0010437823191973314 104-105 0.0 106-107 6.088730195317766E-4 108-109 0.002174546498327774 110-111 1.739637198662219E-4 112-113 4.349092996655548E-4 114-115 0.0 116-117 0.0017831281286287744 118-119 0.002218037428294329 120-121 0.0025224739380602175 122-123 0.0011742551090969978 124-125 0.10033357543284348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1149665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.81163658736919 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18254826704666 51.8039126725334 2 10.61477878966063 13.546928131622776 3 3.3254039622697205 6.365984074396589 4 1.4926654176421477 3.8099769270845756 5 0.877157161335548 2.798641700457618 6 0.5558911062771106 2.1283392753543366 7 0.38256699081650053 1.708855805181416 8 0.2789164528040585 1.4238492259656554 9 0.20316376863743485 1.1667791314811116 >10 1.0294152460960715 11.481690598762833 >50 0.0435457237866313 1.886297774947664 >100 0.013400169715393056 1.3485926265678299 >500 1.3673597802141927E-4 0.05462066932738739 >1k 4.102079340642579E-4 0.4755313863167113 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2320 0.2017979150448174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 0.1620472050553857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.11020601653525157 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 8.698185993311095E-5 0.0 0.0 0.0 4 0.0 8.698185993311095E-5 0.0 0.0 0.0 5 0.0 8.698185993311095E-5 0.0 0.0 0.0 6 0.0 8.698185993311095E-5 0.0 0.0 0.0 7 0.0 8.698185993311095E-5 0.0 0.0 0.0 8 0.0 8.698185993311095E-5 0.0 0.0 0.0 9 0.0 8.698185993311095E-5 0.0 0.0 0.0 10-11 0.0 8.698185993311095E-5 0.0 8.698185993311095E-5 0.0 12-13 0.0 8.698185993311095E-5 0.0 8.698185993311095E-5 0.0 14-15 0.0 8.698185993311095E-5 0.0 1.3047278989966642E-4 0.0 16-17 0.0 8.698185993311095E-5 0.0 1.739637198662219E-4 0.0 18-19 0.0 8.698185993311095E-5 0.0 1.739637198662219E-4 0.0 20-21 0.0 8.698185993311095E-5 0.0 1.739637198662219E-4 0.0 22-23 0.0 8.698185993311095E-5 0.0 2.174546498327774E-4 0.0 24-25 0.0 8.698185993311095E-5 0.0 3.479274397324438E-4 0.0 26-27 0.0 8.698185993311095E-5 0.0 4.7840022963211023E-4 8.698185993311095E-5 28-29 0.0 8.698185993311095E-5 0.0 0.002739928587892995 8.698185993311095E-5 30-31 0.0 8.698185993311095E-5 0.0 0.010872732491638868 8.698185993311095E-5 32-33 0.0 8.698185993311095E-5 0.0 0.02152801033344496 1.739637198662219E-4 34-35 0.0 1.3047278989966642E-4 0.0 0.03592350815237483 1.739637198662219E-4 36-37 0.0 1.739637198662219E-4 0.0 0.05810388243531811 1.739637198662219E-4 38-39 0.0 1.739637198662219E-4 0.0 0.09768062870488359 2.6094557979933284E-4 40-41 0.0 1.739637198662219E-4 0.0 0.14773868909638893 2.6094557979933284E-4 42-43 0.0 1.739637198662219E-4 0.0 0.19218641952220863 2.6094557979933284E-4 44-45 0.0 1.739637198662219E-4 0.0 0.2358078222786638 2.6094557979933284E-4 46-47 0.0 1.739637198662219E-4 0.0 0.28956261171732633 2.6094557979933284E-4 48-49 0.0 1.739637198662219E-4 0.0 0.3458833660240157 3.044365097658883E-4 50-51 0.0 1.739637198662219E-4 0.0 0.4056833947280295 3.479274397324438E-4 52-53 0.0 1.739637198662219E-4 0.0 0.467657969930371 4.349092996655548E-4 54-55 0.0 1.739637198662219E-4 0.0 0.528893199323281 4.349092996655548E-4 56-57 0.0 1.739637198662219E-4 0.0 0.596999995650907 4.349092996655548E-4 58-59 0.0 1.739637198662219E-4 0.0 0.6792848351476299 4.349092996655548E-4 60-61 0.0 1.739637198662219E-4 0.0 0.7620045839440185 5.218911595986657E-4 62-63 0.0 1.739637198662219E-4 0.0 0.850204189916193 5.218911595986657E-4 64-65 0.0 1.739637198662219E-4 0.0 0.9373165226392035 5.218911595986657E-4 66-67 0.0 1.739637198662219E-4 0.0 1.0324311864760602 5.218911595986657E-4 68-69 0.0 1.739637198662219E-4 0.0 1.1378966916449573 5.218911595986657E-4 70-71 0.0 1.739637198662219E-4 0.0 1.25693136696342 5.218911595986657E-4 72-73 0.0 1.739637198662219E-4 0.0 1.367441820008437 6.958548794648876E-4 74-75 0.0 1.739637198662219E-4 0.0 1.4848238399881706 6.958548794648876E-4 76-77 0.0 1.739637198662219E-4 0.0 1.621646305662954 6.958548794648876E-4 78-79 0.0 1.739637198662219E-4 0.0 1.778952999351985 6.958548794648876E-4 80-81 0.0 1.739637198662219E-4 0.0 1.9307798358652302 7.828367393979986E-4 82-83 0.0 1.739637198662219E-4 0.0 2.0951320602088437 8.698185993311095E-4 84-85 0.0 1.739637198662219E-4 0.0 2.2655730147477744 9.568004592642205E-4 86-87 0.0 1.739637198662219E-4 0.0 2.449931066876003 9.568004592642205E-4 88-89 0.0 1.739637198662219E-4 0.0 2.661775386743095 0.0010437823191973314 90-91 0.0 1.739637198662219E-4 0.0 2.884318475381959 0.0010437823191973314 92-93 0.0 1.739637198662219E-4 0.0 3.1096014926087165 0.0010437823191973314 94-95 0.0 1.739637198662219E-4 0.0 3.3393205846920626 0.0010437823191973314 96-97 0.0 1.739637198662219E-4 0.0 3.5955256531250406 0.0011307641791304423 98-99 0.0 1.739637198662219E-4 0.0 3.874998369090126 0.0012177460390635532 100-101 0.0 1.739637198662219E-4 0.0 4.187132773460095 0.0012177460390635532 102-103 0.0 1.739637198662219E-4 0.0 4.5037467436166185 0.0012177460390635532 104-105 0.0 1.739637198662219E-4 0.0 4.839757668538226 0.0012177460390635532 106-107 0.0 1.739637198662219E-4 0.0 5.188989836169666 0.0012177460390635532 108-109 0.0 1.739637198662219E-4 0.0 5.565142889450405 0.0012177460390635532 110-111 0.0 1.739637198662219E-4 0.0 5.972261484867332 0.0013482188289632197 112-113 0.0 1.739637198662219E-4 0.0 6.3925578320641225 0.0013917097589297752 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3380 0.0 47.462917 1 GGTATCA 2085 0.0 45.478897 1 TCAACGC 4065 0.0 38.721184 4 CAACGCA 4250 0.0 37.14196 5 AACGCAG 4395 0.0 36.469826 6 ATCAACG 4330 0.0 36.218456 3 GTACATG 6030 0.0 36.000034 1 TATCAAC 4465 0.0 35.52403 2 TACATGG 6295 0.0 34.38531 2 ACATGGG 6250 0.0 34.06044 3 CATGGGG 5175 0.0 33.29605 4 ATGGGGG 3190 0.0 32.117786 5 ACGCAGA 5375 0.0 29.59873 7 CGCAGAG 5560 0.0 28.61263 8 GTATAGG 365 0.0 27.776333 1 GCAGAGT 6085 0.0 25.948164 9 TGGGGGG 2140 0.0 23.945557 6 GAGTACT 2885 0.0 22.599379 12-13 AGAGTAC 5585 0.0 22.510267 10-11 TACCGTT 160 2.586026E-4 22.344545 7 >>END_MODULE