FastQCFastQC Report
Mon 15 Aug 2016
SRR2921640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587227
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30600.036.3727341
GTACATG79300.034.6005861
TACATGG81000.033.281312
ACATGGG81750.032.3936233
CATGGGG68550.032.2940674
ATGGGGG38700.030.5995945
GGTATCA21850.029.1466121
TCAACGC36100.029.0130044
CAACGCA36750.028.3370295
AACGCAG38750.026.8702356
TATCAAC38800.026.8406852
ATCAACG39150.026.600733
ACCGTAT1300.002054709222.8803848
ACGCAGA45750.022.4960047
GACCGTA1350.00255798422.0336577
GAGTACA58700.021.6985861
GGACCGT4201.8189894E-1221.249426
CGCAGAG48550.021.0754018
ATGGGGA30100.020.363125
AGTACAT58900.020.308042
Produced by FastQC (version 0.11.5)