##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921640_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1587227 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.052891615377007 31.0 31.0 34.0 26.0 34.0 2 30.139574868623075 31.0 31.0 34.0 26.0 34.0 3 30.310862907448023 31.0 31.0 34.0 26.0 34.0 4 33.83425622169986 37.0 35.0 37.0 30.0 37.0 5 33.79692003727255 37.0 35.0 37.0 30.0 37.0 6 33.780244413685 37.0 35.0 37.0 29.0 37.0 7 33.59808962423144 37.0 35.0 37.0 28.0 37.0 8 33.65077521992758 37.0 35.0 37.0 28.0 37.0 9 35.26752002076577 39.0 37.0 39.0 28.0 39.0 10-11 35.326081272558994 39.0 37.0 39.0 29.0 39.0 12-13 35.297843660673614 39.0 37.0 39.0 29.0 39.0 14-15 36.59132751648 40.0 38.0 41.0 28.5 41.0 16-17 36.552030049892046 40.0 38.0 41.0 28.5 41.0 18-19 36.49712170974914 40.0 38.0 41.0 28.0 41.0 20-21 36.55031762942541 40.0 38.0 41.0 28.0 41.0 22-23 36.46154519800885 40.0 38.0 41.0 27.5 41.0 24-25 36.443498630000626 40.0 38.0 41.0 27.5 41.0 26-27 36.404135640333735 40.0 38.0 41.0 28.0 41.0 28-29 36.347049602860835 40.0 38.0 41.0 28.0 41.0 30-31 36.25650111798754 40.0 37.5 41.0 27.0 41.0 32-33 36.17264921778674 40.0 37.0 41.0 27.0 41.0 34-35 36.04554767528526 40.0 37.0 41.0 27.0 41.0 36-37 35.940759891307295 40.0 37.0 41.0 26.0 41.0 38-39 35.82471946356759 40.0 37.0 41.0 26.0 41.0 40-41 35.71530568721424 40.0 36.5 41.0 25.5 41.0 42-43 35.46230249359418 40.0 35.5 41.0 24.5 41.0 44-45 35.10874373986834 40.0 35.0 41.0 24.0 41.0 46-47 34.853963232732305 39.0 35.0 41.0 23.0 41.0 48-49 34.65737761517414 39.0 35.0 41.0 22.5 41.0 50-51 34.45273675410007 39.0 34.5 40.5 22.0 41.0 52-53 34.24767597829422 38.5 34.0 40.0 20.5 41.0 54-55 34.00256327544831 38.0 34.0 40.0 19.5 41.0 56-57 33.74563562741813 38.0 34.0 40.0 18.5 41.0 58-59 33.756714383008855 38.0 34.0 40.0 18.5 41.0 60-61 33.54540812372774 37.5 34.0 40.0 18.0 41.0 62-63 33.240471589760006 37.0 33.0 40.0 16.0 41.0 64-65 32.94245372590058 36.0 33.0 39.5 13.5 41.0 66-67 32.62585124875018 36.0 33.0 39.0 12.5 41.0 68-69 32.29619991343393 35.0 33.0 39.0 9.0 40.5 70-71 31.93340681578627 35.0 32.5 38.0 7.5 40.0 72-73 31.586990707693353 35.0 32.0 37.0 7.0 39.5 74-75 31.228491576819195 35.0 32.0 37.0 4.0 39.0 76-77 30.935532850688652 35.0 32.0 36.0 2.0 39.0 78-79 30.668374151901396 35.0 31.5 36.0 2.0 37.5 80-81 30.41500239096235 35.0 31.0 35.0 2.0 37.0 82-83 30.200744127966573 35.0 31.0 35.0 2.0 37.0 84-85 29.98737389169917 35.0 31.0 35.0 2.0 36.0 86-87 29.824562901210726 35.0 31.0 35.0 2.0 36.0 88-89 29.66870365738486 34.0 31.0 35.0 2.0 35.5 90-91 29.495064978103322 34.0 31.0 35.0 2.0 35.0 92-93 29.42340572583506 34.0 31.0 35.0 2.0 35.0 94-95 29.30735080741444 34.0 30.0 35.0 2.0 35.0 96-97 29.229176418999927 34.0 30.0 35.0 2.0 35.0 98-99 29.150688905871686 34.0 30.0 35.0 2.0 35.0 100-101 29.05158430394644 34.0 30.0 35.0 2.0 35.0 102-103 28.17886477485577 33.5 28.0 34.5 2.0 35.0 104-105 29.11284145241985 34.0 30.5 35.0 2.0 35.0 106-107 29.406315542767356 34.5 31.0 35.0 2.0 35.0 108-109 29.421410737090536 35.0 31.0 35.0 2.0 35.0 110-111 29.37933294985531 35.0 31.0 35.0 2.0 35.0 112-113 29.31202216192139 35.0 31.0 35.0 2.0 35.0 114-115 29.21445262712895 35.0 31.0 35.0 2.0 35.0 116-117 29.107182841521723 35.0 31.0 35.0 2.0 35.0 118-119 28.84151195764689 34.0 31.0 35.0 2.0 35.0 120-121 28.74559089531617 34.0 30.0 35.0 2.0 35.0 122-123 28.5151840284975 34.0 30.0 35.0 2.0 35.0 124-125 27.74302793488266 33.5 28.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68422.0 3 11932.0 4 5648.0 5 4955.0 6 5111.0 7 5578.0 8 5878.0 9 6065.0 10 6488.0 11 6467.0 12 6557.0 13 6441.0 14 6370.0 15 5878.0 16 5123.0 17 4711.0 18 4476.0 19 4183.0 20 4239.0 21 4300.0 22 4992.0 23 6604.0 24 8995.0 25 11572.0 26 13489.0 27 15173.0 28 18109.0 29 21477.0 30 26502.0 31 33256.0 32 43737.0 33 60303.0 34 92307.0 35 157031.0 36 312805.0 37 462592.0 38 119394.0 39 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.38167340017053 17.287806402783897 11.994837542009565 25.335682655036013 2 16.368009265006016 18.959614932639045 38.721159486770084 25.95121631558486 3 20.020427464543353 22.960986819181652 28.27546706874705 28.743118647527943 4 13.67038614386053 14.483907574488786 34.43928392975399 37.406422351896694 5 14.77383071566975 37.61313190188881 32.677641424723134 14.9353959577183 6 35.54706244746544 33.43779814246015 16.407743175473765 14.607396234600637 7 31.434848112357145 30.813996734683585 19.20800317706462 18.543151975894652 8 27.518715573083863 35.43305349530701 18.557805703451532 18.4904252281576 9 27.734200468227638 16.008091578519508 18.119149123884966 38.13855882936789 10-11 26.920802907055535 25.182305537083554 26.138538971373826 21.75835258448709 12-13 26.592721507820887 22.470151830586907 26.641649317720123 24.295477343872083 14-15 24.055966443325218 23.83691032563486 23.5991373877621 28.50798584327783 16-17 24.792533441230123 26.982926530289408 24.2551607848105 23.969379243669966 18-19 24.808790801537615 26.359988226492536 25.526987362387676 23.304233609582173 20-21 25.359708036373192 25.312316254435892 25.313482142688336 24.01449356650258 22-23 25.35633229991977 24.94168534857382 25.198200486433375 24.503781865073037 24-25 24.824939703379066 25.369389672781722 25.214953435946303 24.590717187892906 26-27 24.655729252676576 25.501594032808462 25.35136164059949 24.49131507391547 28-29 24.782003472313047 25.642801789164604 25.315361849496753 24.259832889025592 30-31 25.097458700575864 25.427677640774725 25.185874191625295 24.28898946702411 32-33 24.597693833269577 25.473897285650455 25.319623466005876 24.608785415074088 34-35 24.659155298873735 25.730836211129272 25.309992039446026 24.300016450550967 36-37 24.869137740974796 25.63514074210361 25.2168225777132 24.278898939208396 38-39 24.901498607510902 25.65573334030707 25.235922847376322 24.206845204805703 40-41 24.795442312273966 25.616383271334698 25.238214691345107 24.34995972504623 42-43 24.765513834116636 25.401764727382265 25.300653990171874 24.53206744832923 44-45 24.599990926070156 25.45637456174179 25.476066249770003 24.46756826241805 46-47 24.64078590077177 25.48568972699035 25.393054364354416 24.480470007883458 48-49 24.595073895351 25.623199566919237 25.339762322351493 24.441964215378277 50-51 24.536246068293064 25.663844807626013 25.317041629615716 24.482867494465204 52-53 24.65942913535931 25.696660177802034 25.254014157866578 24.389896528972077 54-55 24.57634969118317 25.362145314371816 25.382720499501215 24.678784494943798 56-57 24.572950933313166 25.53897102380862 25.293700608764695 24.59437743411351 58-59 24.53669162850136 25.57717416235863 25.411479587335755 24.47465462180426 60-61 24.769269985389435 25.470085523946423 25.357914706696942 24.402729783967196 62-63 24.535663050194426 25.567165839193702 25.477743933851738 24.419427176760138 64-65 24.547685319678138 25.326849384300015 25.4573559780023 24.668109318019553 66-67 24.53199098801027 25.647429534748152 25.24135431929543 24.579225157946148 68-69 24.610942418753282 25.50524472715162 25.33935311408333 24.544459740011764 70-71 24.52176335610103 25.343808175255266 25.507025861131655 24.627402607512046 72-73 24.661956187820497 25.47814511110586 25.279800868051456 24.58009783302219 74-75 24.472615712649283 25.542789957393396 25.390938567606636 24.593655762350686 76-77 24.50643981009027 25.34571504277506 25.4220872615613 24.725757885573373 78-79 24.512548949916376 25.336678375574888 25.495643504706884 24.655129169801857 80-81 24.562971682285305 25.575864738384794 25.21992804388573 24.641235535444167 82-83 24.57630002508107 25.590413484794443 25.27066132107932 24.56262516904517 84-85 24.58488036709792 25.627700581427405 25.167662491710207 24.619756559764465 86-87 24.516643713839844 25.615102676485495 25.326372813770814 24.541880795903843 88-89 24.469247233632675 25.407160684200726 25.36232409199599 24.76126799017061 90-91 24.58948470862835 25.363757697358636 25.3216002439965 24.72515735001651 92-93 24.47927807928866 25.53633215812871 25.465542645561918 24.518847117020705 94-95 24.542833919322522 25.2966176452056 25.456031908056403 24.704516527415475 96-97 24.466932116272723 25.356097979008098 25.457799750849563 24.719170153869612 98-99 24.44254091708714 25.363206881429917 25.319952215092574 24.874299986390376 100-101 24.420951750258574 25.381057051520557 25.469805751558454 24.72818544666241 102-103 24.259062483559486 25.312200428248794 25.58542404159879 24.843313046592925 104-105 24.301689001235545 25.424518524072383 25.508095953063066 24.765696521629007 106-107 24.20581830255564 25.396582883672696 25.608715388679105 24.78888342509256 108-109 24.243778983181997 25.37067124884099 25.63169513670122 24.7538546312758 110-111 24.108762395232368 25.598458420252484 25.503038993751904 24.789740190763247 112-113 24.10661238201626 25.60879643193092 25.560408999810356 24.724182186242473 114-115 24.19516280107258 25.612983128372885 25.475051094398378 24.716802976156156 116-117 24.017412496471632 25.472013740473404 25.66286064962297 24.847713113431993 118-119 24.17353270267521 25.39119188825309 25.575233874845132 24.860041534226564 120-121 24.058467281476172 25.572568260393613 25.676366586074646 24.69259787205557 122-123 24.039470505014933 25.58337410192284 25.547840062802003 24.829315330260226 124-125 24.075201765110936 25.458445030047905 25.61171035571144 24.85464284912972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 390.0 1 303.0 2 201.5 3 154.0 4 109.0 5 99.0 6 100.0 7 116.0 8 154.0 9 172.5 10 184.0 11 190.5 12 188.5 13 191.5 14 201.5 15 239.0 16 269.5 17 303.5 18 351.5 19 405.0 20 452.5 21 539.5 22 675.0 23 848.5 24 1041.0 25 1306.5 26 1814.0 27 2433.0 28 3256.0 29 4466.5 30 5998.0 31 7816.0 32 10341.5 33 12830.0 34 15403.0 35 18668.0 36 22258.5 37 25773.5 38 29152.5 39 32934.5 40 36410.0 41 39557.0 42 43097.5 43 47977.0 44 52791.5 45 57304.5 46 60650.5 47 63435.5 48 67418.5 49 69961.0 50 71042.0 51 71033.0 52 69734.5 53 68832.5 54 68380.5 55 66263.0 56 62401.5 57 57380.5 58 52825.0 59 48672.5 60 42316.5 61 35360.5 62 29411.0 63 23164.0 64 17429.5 65 13564.0 66 10666.0 67 8291.0 68 6551.0 69 5112.5 70 4109.5 71 3250.5 72 2458.5 73 2003.5 74 1639.0 75 1273.0 76 946.0 77 659.0 78 457.0 79 316.5 80 204.5 81 144.0 82 106.0 83 90.0 84 71.0 85 60.0 86 48.0 87 35.0 88 27.5 89 24.0 90 31.5 91 21.5 92 11.0 93 14.0 94 14.0 95 9.0 96 6.0 97 6.0 98 6.5 99 6.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024319142756518128 2 0.011340532891640578 3 0.009324438155348919 4 0.01108852104960412 5 0.015876746048296808 6 0.08606204405545016 7 0.05399353715631098 8 0.04498411380350763 9 0.024697160519572815 10-11 0.04674819669776283 12-13 0.012285577299277293 14-15 0.026398240453318903 16-17 0.033108055747539576 18-19 0.038683817752596196 20-21 0.0286033440711379 22-23 0.03628970525324985 24-25 0.03074544472844779 26-27 0.043157027948743314 28-29 0.042621502784415836 30-31 0.025264187164154843 32-33 0.027595296702992076 34-35 0.04120393617296077 36-37 0.009922966280185507 38-39 0.020192448843171142 40-41 0.03988087400226937 42-43 0.022240045059717356 44-45 0.01656977861389707 46-47 0.022681065783281155 48-49 0.028729349992156133 50-51 0.028225326308083217 52-53 0.025579201966700417 54-55 0.022933077625317613 56-57 0.025642204927209533 58-59 0.01707380229796998 60-61 0.022775570224044826 62-63 0.023153587987099513 64-65 0.021042988810044183 66-67 0.02847733815011967 68-69 0.021893528776917227 70-71 0.04381855903408901 72-73 0.02138950509284431 74-75 0.009387441115858035 76-77 0.01653827713364251 78-79 0.025264187164154843 80-81 0.018113351146370367 82-83 0.02350010426989964 84-85 0.011624046213931592 86-87 0.01742031858077011 88-89 0.022271546539971914 90-91 0.02009794440240747 92-93 0.008032877464912077 94-95 0.010521494405022092 96-97 0.014742692759132751 98-99 0.007245340458548147 100-101 0.010174978122221963 102-103 0.004126693913346988 104-105 0.004788224998692688 106-107 7.245340458548147E-4 108-109 0.013073114305641221 110-111 0.001512071052218744 112-113 0.002394112499346344 114-115 0.013451132068695908 116-117 0.005733269406329403 118-119 0.2794811328184311 120-121 9.135429273821578E-4 122-123 0.0010395488484003864 124-125 0.3152353129073535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1587227.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.61124292262414 #Duplication Level Percentage of deduplicated Percentage of total 1 82.119180002313 53.05822287736145 2 9.894652117332182 12.78611543175614 3 3.22648607591624 6.254018269124654 4 1.4767927766003472 3.8166966734120664 5 0.8588428071286461 2.77454506218687 6 0.5341092804215845 2.070567867872819 7 0.34904995905865366 1.5786786187809476 8 0.2623131837822027 1.3558704671327082 9 0.20695165461397796 1.2034263266552467 >10 1.0105959510523035 11.27158563748582 >50 0.046027221487639006 2.038337733165953 >100 0.014317279920086164 1.5452838062061052 >500 6.816903731298968E-4 0.24665122885933632 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.300296050911432E-5 2 0.0 0.0 0.0 0.0 6.300296050911432E-5 3 0.0 0.0 0.0 0.0 6.300296050911432E-5 4 0.0 0.0 0.0 0.0 6.300296050911432E-5 5 0.0 0.0 0.0 0.0 6.300296050911432E-5 6 0.0 0.0 0.0 0.0 6.300296050911432E-5 7 0.0 0.0 0.0 0.0 6.300296050911432E-5 8 0.0 0.0 0.0 0.0 6.300296050911432E-5 9 0.0 0.0 0.0 6.300296050911432E-5 6.300296050911432E-5 10-11 0.0 0.0 0.0 1.8900888152734296E-4 6.300296050911432E-5 12-13 0.0 0.0 0.0 2.520118420364573E-4 1.8900888152734296E-4 14-15 0.0 0.0 0.0 2.520118420364573E-4 2.8351332229101445E-4 16-17 0.0 0.0 0.0 3.1501480254557163E-4 3.1501480254557163E-4 18-19 0.0 0.0 0.0 3.780177630546859E-4 3.1501480254557163E-4 20-21 0.0 0.0 0.0 3.780177630546859E-4 3.4651628280012875E-4 22-23 0.0 0.0 0.0 5.670266445820289E-4 4.410207235638002E-4 24-25 0.0 0.0 0.0 6.300296050911433E-4 5.670266445820289E-4 26-27 0.0 0.0 0.0 7.56035526109372E-4 6.300296050911433E-4 28-29 0.0 0.0 0.0 0.0026461243413828015 6.300296050911433E-4 30-31 0.0 0.0 0.0 0.010395488484003863 6.615310853457005E-4 32-33 0.0 6.300296050911432E-5 0.0 0.0250751782826275 7.560355261093718E-4 34-35 0.0 6.300296050911432E-5 0.0 0.04429108123790737 7.87537006363929E-4 36-37 0.0 6.300296050911432E-5 0.0 0.06886223583646195 8.190384866184862E-4 38-39 0.0 6.300296050911432E-5 0.0 0.11236578006800539 9.135429273821577E-4 40-41 0.0 6.300296050911432E-5 0.0 0.16431172100777014 9.450444076367149E-4 42-43 0.0 6.300296050911432E-5 0.0 0.2148085938558253 9.450444076367149E-4 44-45 0.0 6.300296050911432E-5 0.0 0.2724878042019194 9.450444076367149E-4 46-47 0.0 6.300296050911432E-5 0.0 0.33325415961296023 9.450444076367149E-4 48-49 0.0 6.300296050911432E-5 0.0 0.396792645286402 9.450444076367149E-4 50-51 0.0 6.300296050911432E-5 0.0 0.4631032612222448 9.450444076367149E-4 52-53 0.0 6.300296050911432E-5 0.0 0.5300124052829243 9.450444076367149E-4 54-55 0.0 6.300296050911432E-5 0.0 0.6063404919397162 9.450444076367149E-4 56-57 0.0 6.300296050911432E-5 0.0 0.6882758420818194 9.450444076367149E-4 58-59 0.0 6.300296050911432E-5 0.0 0.7756609483079611 9.450444076367149E-4 60-61 0.0 6.300296050911432E-5 0.0 0.8647156329875941 0.0010080473681458291 62-63 0.0 6.300296050911432E-5 0.0 0.9624017232569758 0.0010080473681458291 64-65 0.0 6.300296050911432E-5 0.0 1.0586072439543934 0.0010395488484003864 66-67 0.0 6.300296050911432E-5 0.0 1.1713510417854536 0.0011025518089095008 68-69 0.0 6.300296050911432E-5 0.0 1.2945533310610267 0.0011340532891640578 70-71 0.0 6.300296050911432E-5 0.0 1.4279936014193306 0.0011340532891640578 72-73 0.0 6.300296050911432E-5 0.0 1.560646334771271 0.0012285577299277293 74-75 0.0 6.300296050911432E-5 0.0 1.7008279219040503 0.0012915606904368437 76-77 0.0 6.300296050911432E-5 0.0 1.8580833113347996 0.0013230621706914008 78-79 0.0 6.300296050911432E-5 0.0 2.032191992701737 0.0013230621706914008 80-81 0.0 6.300296050911432E-5 0.0 2.21622364034886 0.0014805695719641867 82-83 0.0 6.300296050911432E-5 0.0 2.4092647113487864 0.0017010799337460868 84-85 0.0 6.300296050911432E-5 0.0 2.6092361080047155 0.0017010799337460868 86-87 0.0 6.300296050911432E-5 0.0 2.8236666840974856 0.0017010799337460868 88-89 0.0 6.300296050911432E-5 0.0 3.0559585994945904 0.001764082894255201 90-91 0.0 6.300296050911432E-5 0.0 3.3148692657067955 0.001764082894255201 92-93 0.0 6.300296050911432E-5 0.0 3.5790091776412574 0.0018900888152734298 94-95 0.0 6.300296050911432E-5 0.0 3.848693350100521 0.0020160947362916582 96-97 0.0 6.300296050911432E-5 0.0 4.1380344462386285 0.00211059917705533 98-99 0.0 6.300296050911432E-5 0.0 4.471950136936934 0.002142100657309887 100-101 0.0 6.300296050911432E-5 0.0 4.8335556287789965 0.002142100657309887 102-103 0.0 6.300296050911432E-5 0.0 5.208990270452809 0.002205103617819001 104-105 0.0 6.300296050911432E-5 0.0 5.593938359163497 0.0022366050980735584 106-107 0.0 6.300296050911432E-5 0.0 5.993660642113573 0.0022681065783281156 108-109 0.0 6.300296050911432E-5 0.0 6.419182637392131 0.002362611019091787 110-111 0.0 6.300296050911432E-5 0.0 6.8844279992716855 0.0024571154598554586 112-113 0.0 6.300296050911432E-5 0.0 7.358462274142262 0.002520118420364573 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3060 0.0 36.372734 1 GTACATG 7930 0.0 34.600586 1 TACATGG 8100 0.0 33.28131 2 ACATGGG 8175 0.0 32.393623 3 CATGGGG 6855 0.0 32.294067 4 ATGGGGG 3870 0.0 30.599594 5 GGTATCA 2185 0.0 29.146612 1 TCAACGC 3610 0.0 29.013004 4 CAACGCA 3675 0.0 28.337029 5 AACGCAG 3875 0.0 26.870235 6 TATCAAC 3880 0.0 26.840685 2 ATCAACG 3915 0.0 26.60073 3 ACCGTAT 130 0.0020547092 22.880384 8 ACGCAGA 4575 0.0 22.496004 7 GACCGTA 135 0.002557984 22.033657 7 GAGTACA 5870 0.0 21.698586 1 GGACCGT 420 1.8189894E-12 21.24942 6 CGCAGAG 4855 0.0 21.075401 8 ATGGGGA 3010 0.0 20.36312 5 AGTACAT 5890 0.0 20.30804 2 >>END_MODULE