FastQCFastQC Report
Mon 15 Aug 2016
SRR2921640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587227
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.10578197069480294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.045.0331731
GGTATCA25550.042.4662321
CAACGCA50000.034.5522235
TCAACGC50350.034.3315354
AACGCAG52050.033.313196
TATCAAC52600.032.97632
ATCAACG52750.032.882533
GTACATG81950.031.135661
TACATGG83850.029.7855512
ACATGGG85250.028.5272873
ACGCAGA63050.027.4994757
CATGGGG69450.027.4645464
GTATACG1107.6647796E-427.0982071
CGCAGAG66100.026.2297618
ATGGGGG37350.024.2438165
GCAGAGT73200.023.6042259
GTACACT9900.022.2772334
AGAGTAC66300.021.97192810-11
TACACTG16650.020.7520875
GTATAGG5500.020.5946391
Produced by FastQC (version 0.11.5)