##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587227
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73815465588728	33.0	31.0	34.0	30.0	34.0
2	31.91065865185005	33.0	31.0	34.0	30.0	34.0
3	31.977971644887592	34.0	31.0	34.0	30.0	34.0
4	35.37132243844138	37.0	35.0	37.0	33.0	37.0
5	35.22119331387382	37.0	35.0	37.0	33.0	37.0
6	35.246122955317674	37.0	35.0	37.0	33.0	37.0
7	35.13102284676357	37.0	35.0	37.0	33.0	37.0
8	35.17008909248646	37.0	35.0	37.0	33.0	37.0
9	36.8997131475208	39.0	38.0	39.0	34.0	39.0
10-11	36.850016097256415	39.0	38.0	39.0	33.5	39.0
12-13	36.78546294890397	39.0	38.0	39.0	33.0	39.0
14-15	38.21235651863281	41.0	39.0	41.0	34.0	41.0
16-17	38.22073780246934	41.0	39.0	41.0	34.0	41.0
18-19	38.222373989353756	41.0	39.0	41.0	34.0	41.0
20-21	38.159235887494354	41.0	39.0	41.0	34.0	41.0
22-23	38.16101887127676	41.0	39.0	41.0	34.0	41.0
24-25	38.12211580322159	41.0	39.0	41.0	34.0	41.0
26-27	38.05293697750858	41.0	39.0	41.0	33.5	41.0
28-29	37.98625023389849	41.0	39.0	41.0	33.0	41.0
30-31	37.88258705276561	41.0	38.0	41.0	33.0	41.0
32-33	37.78126695173406	41.0	38.0	41.0	33.0	41.0
34-35	37.65758804506224	41.0	38.0	41.0	33.0	41.0
36-37	37.585944228519296	40.5	38.0	41.0	33.0	41.0
38-39	37.501993728685314	40.0	38.0	41.0	32.0	41.0
40-41	37.39179871562165	40.0	38.0	41.0	32.0	41.0
42-43	37.24132181471207	40.0	38.0	41.0	31.5	41.0
44-45	37.103171443026106	40.0	37.5	41.0	31.0	41.0
46-47	36.924988360203045	40.0	37.0	41.0	31.0	41.0
48-49	36.75780968947731	40.0	37.0	41.0	31.0	41.0
50-51	36.52189226871771	40.0	36.0	41.0	30.0	41.0
52-53	36.398884658590106	40.0	36.0	41.0	30.0	41.0
54-55	36.26883174240358	40.0	35.5	41.0	30.0	41.0
56-57	36.07118515499043	39.0	35.0	41.0	30.0	41.0
58-59	35.87599757312596	39.0	35.0	41.0	29.5	41.0
60-61	35.6252968226977	39.0	35.0	41.0	29.0	41.0
62-63	35.28117654248573	38.0	35.0	41.0	28.5	41.0
64-65	34.99379011319742	37.0	35.0	40.0	29.0	41.0
66-67	34.69312612499661	37.0	35.0	39.5	29.0	41.0
68-69	34.32833898364884	36.0	35.0	39.0	28.0	41.0
70-71	33.9565137815826	36.0	35.0	39.0	28.0	41.0
72-73	33.63653749589693	35.0	34.0	37.5	28.0	40.0
74-75	33.31868377995082	35.0	34.0	37.0	27.5	39.0
76-77	32.98913639951942	35.0	34.0	37.0	27.0	39.0
78-79	32.726446185706266	35.0	34.0	36.0	26.5	38.5
80-81	32.47520833503967	35.0	34.0	36.0	26.0	37.0
82-83	32.24860432691732	35.0	34.0	35.5	26.0	37.0
84-85	32.03333612646458	35.0	34.0	35.0	26.0	36.5
86-87	31.90360736050987	35.0	34.0	35.0	26.0	36.0
88-89	31.806741253771513	35.0	34.0	35.0	25.0	36.0
90-91	31.699929814701996	35.0	34.0	35.0	25.0	36.0
92-93	31.623920523025376	35.0	33.0	35.0	25.0	35.0
94-95	31.555315969297396	35.0	33.0	35.0	25.0	35.0
96-97	31.490056557757647	35.0	33.0	35.0	25.0	35.0
98-99	31.414095778360625	35.0	33.0	35.0	24.0	35.0
100-101	31.347365247692988	35.0	33.0	35.0	24.0	35.0
102-103	30.649781663240354	34.0	31.5	35.0	23.0	35.0
104-105	31.175051835685757	35.0	33.0	35.0	23.5	35.0
106-107	31.279275743167172	35.0	33.0	35.0	24.0	35.0
108-109	31.269408534507036	35.0	33.0	35.0	24.0	35.0
110-111	31.204091475258423	35.0	33.0	35.0	23.0	35.0
112-113	31.169540651715224	35.0	33.0	35.0	23.0	35.0
114-115	31.04409104683829	35.0	33.0	35.0	20.0	35.0
116-117	31.228339739684365	35.0	33.0	35.0	23.5	35.0
118-119	31.217576628925794	35.0	34.0	35.0	23.0	35.0
120-121	31.145632603276027	35.0	34.0	35.0	22.5	35.0
122-123	31.01609851646929	35.0	33.0	35.0	19.0	35.0
124-125	30.373357119051022	34.5	32.0	35.0	8.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26015.0
3	6993.0
4	3756.0
5	3057.0
6	2967.0
7	3281.0
8	3682.0
9	3904.0
10	4260.0
11	4460.0
12	4857.0
13	5797.0
14	7288.0
15	6482.0
16	4240.0
17	3725.0
18	3518.0
19	3272.0
20	3183.0
21	3191.0
22	3586.0
23	4451.0
24	5783.0
25	7491.0
26	9320.0
27	11053.0
28	13472.0
29	16169.0
30	19655.0
31	24661.0
32	32264.0
33	44129.0
34	67631.0
35	121156.0
36	286368.0
37	591688.0
38	220099.0
39	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.498645452672136	17.784428156687632	11.085673306752824	27.631253083887408
2	15.600524948604559	19.49782100966291	37.51920831802863	27.382445723703896
3	18.744981830602985	23.162609485899967	28.062548606130584	30.029860077366465
4	13.233219840521034	14.693711404292111	34.12565831616215	37.9474104390247
5	14.7460021778804	37.25870648020246	32.71660331320138	15.278688028715756
6	35.21385324177861	33.85698651139685	16.130468003338738	14.798692243485803
7	30.989204801171926	31.183664618902878	19.029768425776865	18.797362154148328
8	28.12882113134481	35.03528361776207	18.080238400778534	18.755656850114587
9	27.740909640923256	15.479323592582508	18.15424872190459	38.62551804458965
10-11	27.025267062295832	24.932737998429083	26.070329451427	21.97166548784808
12-13	26.85037399567065	22.101612348097746	26.830107754626564	24.217905901605036
14-15	24.03176002972775	23.54061547664786	23.915521750079506	28.512102743544883
16-17	24.468429434198455	26.850337046640043	24.53234365047874	24.14888986868276
18-19	24.530893025395713	25.931265500083505	26.259370952646556	23.278470521874222
20-21	25.098858441014197	24.983032583111985	26.045567833432585	23.87254114244123
22-23	24.886094970474318	24.612842608970954	25.96083298811885	24.540229432435883
24-25	24.447601750939974	24.99046244914225	26.000198469266405	24.561737330651372
26-27	24.324160851827934	25.214106825019293	26.088868587269836	24.372863735882934
28-29	24.43982402502587	25.429549367343824	25.971241039405353	24.159385568224955
30-31	24.770332959716832	25.08264611208873	25.90041261626687	24.246608311927563
32-33	24.28700550661383	25.12712720092068	26.035927985248485	24.549939307217006
34-35	24.46040241816332	25.192025983599585	26.108918158591948	24.23865343964515
36-37	24.545703709537687	25.270882885721036	25.934535717098527	24.24887768764275
38-39	24.59210932528557	25.32837908504968	25.96976417442146	24.10974741524329
40-41	24.585088416498365	25.19179511024458	25.94246823996464	24.28064823329241
42-43	24.46750934370991	25.085781935578176	25.954847766204374	24.491860954507537
44-45	24.40424585482891	25.16989593913107	25.966009471076422	24.459848734963597
46-47	24.502112897463828	25.164334719249577	25.792123904566466	24.541428478720125
48-49	24.394276180827525	25.387319240520366	25.713650270414668	24.50475430823744
50-51	24.444517949032967	25.452990402925902	25.64461686520781	24.45787478283332
52-53	24.57119779840374	25.46871495427202	25.546965370725523	24.413121876598716
54-55	24.38301202283848	25.168982563670887	25.722145284680803	24.725860128809828
56-57	24.429929152635278	25.32637355367735	25.623088239463463	24.62060905422391
58-59	24.41857314946422	25.326452102297353	25.761774074858117	24.493200673380308
60-61	24.609412900242308	25.273842540381775	25.68837078021655	24.42837377915936
62-63	24.449230616083618	25.38385716081316	25.774731344877477	24.392180878225744
64-65	24.351974970175963	25.09959983657027	25.81153351459291	24.736891678660857
66-67	24.306059597975764	25.473201324623478	25.586894393032118	24.633844684368636
68-69	24.360918172261727	25.294595545603837	25.691264309230267	24.65322197290417
70-71	24.268527522694463	25.191647190870363	25.82340957639001	24.716415710045165
72-73	24.411145966495567	25.311855584040504	25.559525527141314	24.717472922322617
74-75	24.26303969172309	25.418133005532894	25.626274104216723	24.692553198527293
76-77	24.256632579449512	25.156044941951343	25.66725869343745	24.920063785161698
78-79	24.131392839704517	25.150404006993337	25.83379798075257	24.884405172549577
80-81	24.31022783760609	25.41300015687737	25.459685350614624	24.817086654901914
82-83	24.267737610387517	25.40856612161781	25.468831908544697	24.854864359449977
84-85	24.28226571391939	25.351034951812927	25.434578324904965	24.932121009362717
86-87	24.129976367589514	25.38187669438587	25.664350467828484	24.823796470196136
88-89	24.162720800247225	25.17755217568947	25.608497176963724	25.05122984709958
90-91	24.092830406932386	25.185529136847702	25.690060023298646	25.03158043292127
92-93	24.020855239987725	25.394508787967947	25.653072937897353	24.931563034146976
94-95	24.058321102509893	25.14102856105977	25.682303534227565	25.11834680220278
96-97	23.957915932671543	25.141349190148212	25.740484524988595	25.160250352191653
98-99	23.976559000364787	25.209438647092806	25.560555915724297	25.253446436818106
100-101	23.8737748286792	25.124446597745624	25.749782482278842	25.25199609129633
102-103	23.80020073046737	25.02739095125198	25.799125883708633	25.37328243457201
104-105	23.884390827524985	25.115153661070533	25.71988757751727	25.280567933887216
106-107	23.823383021887327	25.12528193949169	25.709699978578897	25.341635060042083
108-109	23.760816716645103	25.08372530099683	25.752992169467383	25.40246581289069
110-111	23.654815491046804	25.273645569803683	25.663224498834918	25.4083144403146
112-113	23.61419170016069	25.29501988850342	25.677322695890027	25.41346571544587
114-115	23.601791048161353	25.28954585575976	25.667941636577503	25.440721459501383
116-117	23.440233291257154	25.195619583033146	25.776926103538905	25.587221022170798
118-119	23.596403961463995	25.02090170622187	25.729638083885302	25.65305624842883
120-121	23.32468908752173	25.184934566461457	25.867244041802362	25.623132304214447
122-123	23.27130843264794	25.277499162052706	25.67108237588929	25.780110029410057
124-125	23.1848027933315	25.192202359498584	25.699854348435498	25.923140498734416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	17.5
2	20.5
3	47.0
4	45.5
5	43.0
6	105.0
7	270.0
8	460.0
9	520.5
10	444.5
11	351.0
12	336.5
13	388.5
14	445.0
15	467.5
16	517.5
17	556.5
18	570.5
19	625.0
20	661.0
21	754.5
22	869.5
23	1004.5
24	1176.5
25	1448.5
26	1939.5
27	2554.5
28	3325.0
29	4448.0
30	5981.0
31	7757.0
32	10101.0
33	12629.0
34	15164.5
35	18218.5
36	21731.5
37	25278.5
38	28788.0
39	32299.5
40	35827.0
41	39343.0
42	42825.0
43	47916.0
44	53060.5
45	57706.0
46	60852.0
47	63214.5
48	67820.0
49	70656.5
50	71693.0
51	71938.0
52	70868.0
53	69907.5
54	69388.5
55	67339.0
56	62627.5
57	57359.5
58	53153.5
59	49022.0
60	42367.5
61	35305.0
62	29177.5
63	22622.0
64	16908.0
65	12879.5
66	9931.5
67	7693.5
68	6020.0
69	4633.5
70	3582.0
71	2830.0
72	2228.5
73	1767.0
74	1382.0
75	1067.5
76	778.5
77	454.5
78	262.5
79	185.0
80	108.0
81	58.5
82	33.5
83	19.0
84	10.0
85	7.5
86	6.0
87	4.0
88	3.5
89	3.5
90	2.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022051036178190014
2	0.001512071052218744
3	0.03106045953099336
4	0.16960396969053576
5	0.0228700746648085
6	0.06293995754860521
7	0.04920531215761828
8	0.04126693913346988
9	0.03093445360997513
10-11	0.05626164373463909
12-13	0.053237501630201606
14-15	0.05232395870281945
16-17	0.045708647849362447
18-19	0.03414760459593996
20-21	0.022807071704299384
22-23	0.003118646545201159
24-25	0.005008735360474589
26-27	0.0032761539464739448
28-29	0.004378705755383445
30-31	0.002394112499346344
32-33	0.00853690114898499
34-35	0.004536213156656231
36-37	0.006426301971929661
38-39	0.0023311095388372297
40-41	0.003244652466219388
42-43	0.003969186512074202
44-45	0.004977233880220032
46-47	0.004410207235638003
48-49	0.0022051036178190016
50-51	0.001543572532473301
52-53	9.450444076367148E-4
54-55	0.0051032398012382605
56-57	7.56035526109372E-4
58-59	6.930325656002575E-4
60-61	6.300296050911432E-5
62-63	9.76545887891272E-4
64-65	3.150148025455716E-5
66-67	0.0037171746700377455
68-69	5.355251643274718E-4
70-71	3.150148025455716E-5
72-73	0.002142100657309887
74-75	0.005197744242001931
76-77	0.001512071052218744
78-79	1.2600592101822864E-4
80-81	0.0
82-83	0.005733269406329403
84-85	0.0017325814140006438
86-87	0.0
88-89	0.0011025518089095008
90-91	6.300296050911433E-4
92-93	0.0
94-95	0.0030556435846920446
96-97	0.0014490680917096294
98-99	5.040236840729146E-4
100-101	0.0
102-103	0.001512071052218744
104-105	0.0
106-107	4.4102072356380027E-4
108-109	0.0026776258216373587
110-111	3.150148025455716E-5
112-113	2.2051036178190013E-4
114-115	0.0
116-117	0.0016695784534915296
118-119	0.0022681065783281156
120-121	0.00277213026240103
122-123	9.450444076367148E-4
124-125	0.10039521757127368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1587227.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.20372629740132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69485886505646	48.776223292869425
2	11.085761651070438	13.569798437806856
3	3.6346178857714913	6.673564748291932
4	1.7621460153866737	4.313996096871292
5	0.982223319255082	3.005786359730654
6	0.6479394189182281	2.379378411166109
7	0.4285365166060168	1.8359622169557495
8	0.30466994232349043	1.491754860880956
9	0.22727638133048803	1.2519145293133498
>10	1.1625049067832554	12.301098878153006
>50	0.049661114834741726	2.0689034317739665
>100	0.018875778109934717	1.8542920420631763
>500	7.219368751237676E-4	0.289286992748995
>1k	2.062676786067907E-4	0.18803970137448311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.10578197069480294	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.300296050911432E-5	0.0	0.0	0.0
6	0.0	6.300296050911432E-5	0.0	0.0	1.2600592101822864E-4
7	0.0	6.300296050911432E-5	0.0	0.0	1.2600592101822864E-4
8	0.0	6.300296050911432E-5	0.0	0.0	1.2600592101822864E-4
9	0.0	6.300296050911432E-5	0.0	0.0	2.520118420364573E-4
10-11	0.0	6.300296050911432E-5	0.0	0.0	2.520118420364573E-4
12-13	0.0	6.300296050911432E-5	0.0	6.300296050911432E-5	2.8351332229101445E-4
14-15	0.0	6.300296050911432E-5	0.0	6.300296050911432E-5	4.095192433092431E-4
16-17	0.0	6.300296050911432E-5	0.0	6.300296050911432E-5	5.040236840729146E-4
18-19	0.0	6.300296050911432E-5	0.0	1.2600592101822864E-4	5.040236840729146E-4
20-21	0.0	6.300296050911432E-5	0.0	1.2600592101822864E-4	5.355251643274718E-4
22-23	0.0	6.300296050911432E-5	0.0	2.520118420364573E-4	5.98528124836586E-4
24-25	0.0	6.300296050911432E-5	0.0	3.1501480254557163E-4	6.300296050911433E-4
26-27	0.0	6.300296050911432E-5	0.0	4.410207235638002E-4	7.560355261093718E-4
28-29	0.0	6.300296050911432E-5	0.0	0.002299608058582673	7.560355261093718E-4
30-31	0.0	6.300296050911432E-5	0.0	0.009922966280185505	7.560355261093718E-4
32-33	0.0	6.300296050911432E-5	0.0	0.024571154598554586	7.560355261093718E-4
34-35	0.0	6.300296050911432E-5	0.0	0.04347204275128888	8.190384866184862E-4
36-37	0.0	6.300296050911432E-5	0.0	0.06842121511289816	8.190384866184862E-4
38-39	0.0	6.300296050911432E-5	0.0	0.11286980375207831	8.190384866184862E-4
40-41	0.0	6.300296050911432E-5	0.0	0.16598129946126167	8.190384866184862E-4
42-43	0.0	6.300296050911432E-5	0.0	0.2169191930328806	8.505399668730434E-4
44-45	0.0	6.300296050911432E-5	0.0	0.27541744186559325	9.135429273821577E-4
46-47	0.0	6.300296050911432E-5	0.0	0.33630980319765225	9.450444076367149E-4
48-49	0.0	6.300296050911432E-5	0.0	0.4005413214366943	0.0010710503286549436
50-51	0.0	9.450444076367148E-5	0.0	0.46675743293177346	0.0011340532891640578
52-53	0.0	1.2600592101822864E-4	0.0	0.5335405710714347	0.001197056249673172
54-55	0.0	1.2600592101822864E-4	0.0	0.6102466754912813	0.001197056249673172
56-57	0.0	1.2600592101822864E-4	0.0	0.6923080315544028	0.001197056249673172
58-59	0.0	1.2600592101822864E-4	0.0	0.7799766511028353	0.001197056249673172
60-61	0.0	1.2600592101822864E-4	0.0	0.8699133772295959	0.0012600592101822865
62-63	0.0	1.2600592101822864E-4	0.0	0.9684500074658509	0.0014490680917096294
64-65	0.0	1.2600592101822864E-4	0.0	1.0651910533275957	0.0014490680917096294
66-67	0.0	1.2600592101822864E-4	0.0	1.177934851158656	0.0014490680917096294
68-69	0.0	1.2600592101822864E-4	0.0	1.3020506833616112	0.0014490680917096294
70-71	0.0	1.2600592101822864E-4	0.0	1.4379165676995163	0.0014805695719641867
72-73	0.0	1.2600592101822864E-4	0.0	1.572238879504948	0.001543572532473301
74-75	0.0	1.2600592101822864E-4	0.0	1.7141845495319825	0.0015750740127278581
76-77	0.0	1.2600592101822864E-4	0.0	1.8732040218569872	0.0016065754929824152
78-79	0.0	1.2600592101822864E-4	0.0	2.05011633496658	0.001764082894255201
80-81	0.0	1.2600592101822864E-4	0.0	2.237802154323232	0.0019215902955279868
82-83	0.0	1.2600592101822864E-4	0.0	2.4333633437435225	0.0020160947362916582
84-85	0.0	1.2600592101822864E-4	0.0	2.6374929357930528	0.0020160947362916582
86-87	0.0	1.2600592101822864E-4	0.0	2.857656781292153	0.0020475962165462155
88-89	0.0	1.2600592101822864E-4	0.0	3.094453408365659	0.0020790976968007727
90-91	0.0	1.2600592101822864E-4	0.0	3.3583098069778297	0.0020790976968007727
92-93	0.0	1.2600592101822864E-4	0.0	3.628781516443458	0.0022681065783281156
94-95	0.0	1.2600592101822864E-4	0.0	3.9044824716313418	0.0022681065783281156
96-97	0.0	1.2600592101822864E-4	0.0	4.199588338656033	0.002394112499346344
98-99	0.0	1.2600592101822864E-4	0.0	4.53662267589954	0.0024571154598554586
100-101	0.0	1.2600592101822864E-4	0.0	4.901220808365785	0.0024571154598554586
102-103	0.0	1.2600592101822864E-4	0.0	5.281065657275235	0.0024571154598554586
104-105	0.0	1.2600592101822864E-4	0.0	5.669352902892907	0.0024571154598554586
106-107	0.0	1.2600592101822864E-4	0.0	6.073012870874802	0.002488616940110016
108-109	0.0	1.2600592101822864E-4	0.0	6.5023780467444166	0.0025516199006191303
110-111	0.0	1.2600592101822864E-4	0.0	6.973325176550047	0.0026146228611282443
112-113	0.0	1.2600592101822864E-4	0.0	7.455486833326298	0.0026461243413828015
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3945	0.0	45.033173	1
GGTATCA	2555	0.0	42.466232	1
CAACGCA	5000	0.0	34.552223	5
TCAACGC	5035	0.0	34.331535	4
AACGCAG	5205	0.0	33.31319	6
TATCAAC	5260	0.0	32.9763	2
ATCAACG	5275	0.0	32.88253	3
GTACATG	8195	0.0	31.13566	1
TACATGG	8385	0.0	29.785551	2
ACATGGG	8525	0.0	28.527287	3
ACGCAGA	6305	0.0	27.499475	7
CATGGGG	6945	0.0	27.464546	4
GTATACG	110	7.6647796E-4	27.098207	1
CGCAGAG	6610	0.0	26.229761	8
ATGGGGG	3735	0.0	24.243816	5
GCAGAGT	7320	0.0	23.604225	9
GTACACT	990	0.0	22.277233	4
AGAGTAC	6630	0.0	21.971928	10-11
TACACTG	1665	0.0	20.752087	5
GTATAGG	550	0.0	20.594639	1
>>END_MODULE