FastQCFastQC Report
Mon 15 Aug 2016
SRR2921639_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921639_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508467
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG3900.074.761122
TACGGTC1850.054.6773035
GTACGGT2300.049.153644
AGTACGG4400.048.683273
GTATCAA31350.039.4872251
GGTATCA20850.036.251711
CTAGTAC4500.035.709361
GTACATG76800.034.252471
TCAACGC36050.033.0106964
TACATGG78450.032.7668842
ACATGGG81000.031.7342823
CAACGCA37500.031.575615
CATGGGG63350.031.1832494
ATCAACG39400.030.3549673
GTATAGG3800.029.7577971
AACGCAG40500.029.3806696
TATCAAC41150.029.2096162
TATCACG1852.86891E-828.9477672
TATTACG1254.881507E-528.56182
GTACGGA2404.3655746E-1027.2716464
Produced by FastQC (version 0.11.5)