##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921639_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1508467 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.029750733691888 31.0 31.0 34.0 26.0 34.0 2 30.12225458031233 31.0 31.0 34.0 26.0 34.0 3 30.12499577385518 31.0 31.0 34.0 26.0 34.0 4 33.54522041251151 37.0 35.0 37.0 28.0 37.0 5 33.69494128807591 37.0 35.0 37.0 28.0 37.0 6 33.571004204931235 37.0 35.0 37.0 28.0 37.0 7 33.575879353012034 37.0 35.0 37.0 28.0 37.0 8 33.54869148612465 37.0 35.0 37.0 28.0 37.0 9 35.156014019531085 39.0 37.0 39.0 28.0 39.0 10-11 35.20417616030049 39.0 37.0 39.0 28.0 39.0 12-13 35.160611402171874 39.0 37.0 39.0 27.0 39.0 14-15 36.4572768247499 40.0 38.0 41.0 27.0 41.0 16-17 36.42843529225366 40.5 38.0 41.0 27.0 41.0 18-19 36.38231131340626 40.0 38.0 41.0 27.0 41.0 20-21 36.432898432647185 40.0 38.0 41.0 27.0 41.0 22-23 36.35485893957242 40.0 38.0 41.0 27.0 41.0 24-25 36.34887571289262 40.0 38.0 41.0 27.0 41.0 26-27 36.309226850835984 40.0 38.0 41.0 27.0 41.0 28-29 36.249872552730686 40.0 38.0 41.0 27.0 41.0 30-31 36.151952942954665 40.0 37.0 41.0 27.0 41.0 32-33 36.05511423186586 40.0 37.0 41.0 27.0 41.0 34-35 35.922255176944546 40.0 37.0 41.0 26.0 41.0 36-37 35.81555977028334 40.0 37.0 41.0 25.0 41.0 38-39 35.69696652296669 40.0 37.0 41.0 25.0 41.0 40-41 35.58842884862579 40.0 36.0 41.0 24.5 41.0 42-43 35.3403959118761 40.0 35.5 41.0 24.0 41.0 44-45 35.00027544520364 40.0 35.0 41.0 23.0 41.0 46-47 34.75298233239441 39.5 35.0 41.0 21.5 41.0 48-49 34.55988463784756 39.0 35.0 41.0 20.5 41.0 50-51 34.3594851594367 39.0 35.0 41.0 19.5 41.0 52-53 34.160879223741716 39.0 34.0 40.5 18.0 41.0 54-55 33.92069896126333 38.0 34.0 40.0 16.0 41.0 56-57 33.67796312415187 38.0 34.0 40.0 13.5 41.0 58-59 33.68487742854169 38.0 34.0 40.0 13.5 41.0 60-61 33.47647479195766 37.5 34.0 40.0 12.5 41.0 62-63 33.17440553886827 37.0 33.5 40.0 8.0 41.0 64-65 32.87799070181847 36.5 33.0 39.5 7.5 41.0 66-67 32.56158338233452 36.0 33.0 39.0 6.5 41.0 68-69 32.23197226057978 35.5 33.0 39.0 2.0 41.0 70-71 31.86457178048973 35.0 32.5 38.5 2.0 40.0 72-73 31.518717346816338 35.0 32.0 37.0 2.0 39.5 74-75 31.157806236397615 35.0 32.0 37.0 2.0 39.0 76-77 30.859099005811863 35.0 32.0 36.0 2.0 39.0 78-79 30.582713774978174 35.0 32.0 36.0 2.0 37.5 80-81 30.321554598145006 35.0 31.0 35.5 2.0 37.0 82-83 30.094451850786264 35.0 31.0 35.0 2.0 37.0 84-85 29.877721554399265 35.0 31.0 35.0 2.0 36.0 86-87 29.706369778059447 35.0 31.0 35.0 2.0 36.0 88-89 29.542386078051425 35.0 31.0 35.0 2.0 36.0 90-91 29.359509356187438 34.0 31.0 35.0 2.0 35.0 92-93 29.282160630626986 34.0 30.5 35.0 2.0 35.0 94-95 29.160472850914207 34.0 30.0 35.0 2.0 35.0 96-97 29.08239954868088 34.0 30.0 35.0 2.0 35.0 98-99 28.991485726900223 34.0 30.0 35.0 2.0 35.0 100-101 28.885435345950558 34.0 30.0 35.0 2.0 35.0 102-103 28.021768789108414 33.5 28.0 34.5 2.0 35.0 104-105 28.939677831865065 34.0 30.5 35.0 2.0 35.0 106-107 29.21846384441953 35.0 31.0 35.0 2.0 35.0 108-109 29.224547835650366 35.0 31.0 35.0 2.0 35.0 110-111 29.172205954787213 35.0 31.0 35.0 2.0 35.0 112-113 29.09652249601748 35.0 31.0 35.0 2.0 35.0 114-115 28.986766034656377 35.0 31.0 35.0 2.0 35.0 116-117 28.87354380307955 35.0 31.0 35.0 2.0 35.0 118-119 28.59859844464612 34.5 30.0 35.0 2.0 35.0 120-121 28.474765772138205 34.0 30.0 35.0 2.0 35.0 122-123 28.232906652913186 34.0 30.0 35.0 2.0 35.0 124-125 27.463456608596676 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68810.0 3 11513.0 4 5742.0 5 5245.0 6 5535.0 7 5900.0 8 5490.0 9 5984.0 10 6614.0 11 6828.0 12 6744.0 13 6821.0 14 6728.0 15 5971.0 16 5104.0 17 4535.0 18 4220.0 19 4095.0 20 4041.0 21 4228.0 22 4833.0 23 6317.0 24 9115.0 25 11209.0 26 12441.0 27 13798.0 28 16199.0 29 19650.0 30 23440.0 31 29937.0 32 39546.0 33 54060.0 34 83771.0 35 141092.0 36 286303.0 37 449517.0 38 127018.0 39 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.679761896078524 17.8718517108829 11.924425810019489 25.523960583019083 2 16.5387642203763 19.8747473167006 38.354954647947274 25.231533814975826 3 19.364327979712595 24.711451444483117 28.328443803556922 27.595776772247362 4 14.091741148531236 15.646926458703092 34.901270230436864 35.36006216232881 5 14.470652588307448 37.56641084718792 33.25843292612177 14.704503638382867 6 33.12124606193152 35.67432206831205 16.754540556465333 14.449891313291097 7 29.47792379390765 32.01845852602421 19.811607353694225 18.69201032637391 8 28.07340556981906 34.19909227960123 19.231919709922117 18.495582440657596 9 27.675450724202342 16.328188356929672 18.387704043504524 37.60865687536346 10-11 26.16095323687326 25.81927499079727 26.55887590742221 21.460895864907258 12-13 26.03087896379708 23.50332432525612 26.72534735021972 23.74044936072708 14-15 23.783059564491868 24.765484271291257 23.940248843791785 27.51120732042509 16-17 24.2854807621848 27.670662476885077 24.566026362201246 23.477830398728873 18-19 24.22860451289282 26.903929506899125 25.998326740549615 22.869139239658438 20-21 24.872845502145875 25.807422772292988 25.824730372783165 23.49500135277797 22-23 24.976589990596207 25.425690794229332 25.503779423331224 24.093939791843237 24-25 24.44323681268327 25.92377198438729 25.621418273981067 24.01157292894837 26-27 24.130291396776542 26.085836252341586 25.7529316923881 24.030940658493773 28-29 24.264841939110163 26.087060329419252 25.801579116662964 23.846518614807618 30-31 24.601560536606033 25.88465662425708 25.676078911404037 23.837703927732846 32-33 24.093339579932767 26.087675863822806 25.659763909045 24.159220647199426 34-35 24.265120394111147 26.206129733558477 25.756964792366087 23.771785079964292 36-37 24.504335592566342 26.08373239525329 25.584703337269254 23.82722867491111 38-39 24.380310916215205 26.195408895170292 25.58456339548541 23.83971679312909 40-41 24.306402744843552 26.00001127436465 25.76633348708472 23.92725249370708 42-43 24.309959904173162 25.87526862612614 25.646078572437386 24.168692897263313 44-45 24.12425434068699 25.8763092342078 25.892354542977223 24.10708188212798 46-47 24.0767208634081 26.00402555107646 25.874261040178265 24.04499254533718 48-49 24.08223770807596 26.20714396074913 25.711667372879948 23.998950958294955 50-51 24.08066276031435 26.161552965687957 25.68710124587507 24.07068302812262 52-53 24.21106934778594 26.026663324716537 25.6617674606119 24.100499866885617 54-55 24.025113111445815 25.913204806063643 25.742464292854127 24.319217789636415 56-57 24.039638204736384 26.062825805215272 25.556125444726298 24.34141054532205 58-59 24.0187122544978 26.009052455908808 25.74045581863793 24.231779470955463 60-61 24.166916423761315 26.074110223773516 25.78613808340384 23.972835269061328 62-63 24.12792095134751 26.11643722758149 25.68550749286619 24.070134328204812 64-65 23.998486905968036 25.86750945518006 25.75439208364055 24.379611555211355 66-67 24.03686752676307 26.091245106256565 25.598886772834817 24.273000594145543 68-69 24.06918085528209 26.005746173748484 25.676730317067314 24.248342653902107 70-71 23.96890362152167 25.873744166859 25.880840768183884 24.276511443435446 72-73 24.13401435015519 26.042096971296374 25.67376173384779 24.15012694470065 74-75 24.05451178104061 26.047836206093542 25.612654222256403 24.284997790609445 76-77 24.0469740965519 25.820238285068875 25.77379328360856 24.358994334770664 78-79 24.09264230557426 25.858372264731518 25.777475852918386 24.271509576775834 80-81 24.15773546674305 26.026358643041092 25.578870385546704 24.237035504669155 82-83 24.22951421881869 25.96407284098542 25.68050542832836 24.125907511867524 84-85 24.13878150154578 25.939651223000215 25.624262828524053 24.297304446929953 86-87 24.09091752949164 25.999155297886784 25.64025635449376 24.26967081812781 88-89 24.035433096971232 25.77466385702104 25.73100074396375 24.458902302043978 90-91 24.1901844456932 25.75221253607441 25.72124780569797 24.336355212534418 92-93 24.19806034175562 25.87723674444769 25.760155825024356 24.16454708877233 94-95 24.108623061018758 25.657620159404786 25.87107260267116 24.3626841769053 96-97 24.109585680571023 25.637090007621445 25.842560672357827 24.410763639449705 98-99 23.979275744964372 25.69045215803073 25.707656074159154 24.62261602284574 100-101 23.947816372770504 25.902531114761217 25.80825450706163 24.34139800540665 102-103 23.775650302669096 25.730088510242222 26.003057518067003 24.49120366902168 104-105 23.674775673642028 25.852174979531362 25.93172921065629 24.541320136170324 106-107 23.71954994981617 25.88312272034812 25.855710548680968 24.541616781154747 108-109 23.74072934664851 25.86613395356485 25.90064405597466 24.492492643811982 110-111 23.70405579515084 26.142524776317888 25.774961193519292 24.378458235011983 112-113 23.555827949989176 26.196348728664535 25.910058348611326 24.33776497273496 114-115 23.45902348547759 26.3105639067211 25.892370244766777 24.338042363034532 116-117 23.501970986129507 26.20012702352587 25.81454830407682 24.483353686267805 118-119 23.56694270363335 26.034723157025315 25.843508145666423 24.554825993674914 120-121 23.453172643053254 26.324165095241774 25.869559524823654 24.353102736881326 122-123 23.569526905728523 26.246874279062244 25.784811183915696 24.39878763129354 124-125 23.41804369633009 26.15768409650019 25.91041224941573 24.513859957753986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 937.0 1 689.5 2 395.5 3 305.5 4 227.0 5 183.5 6 190.5 7 227.5 8 270.5 9 301.0 10 297.0 11 291.0 12 298.5 13 317.0 14 350.5 15 398.5 16 478.0 17 545.0 18 594.5 19 670.0 20 767.0 21 866.5 22 989.0 23 1172.5 24 1426.0 25 1790.5 26 2329.5 27 3141.5 28 4093.5 29 5493.5 30 7568.0 31 9980.5 32 13352.0 33 16421.5 34 19041.5 35 22137.5 36 25606.0 37 29067.0 38 31840.5 39 34829.5 40 37858.0 41 40566.5 42 43101.0 43 46895.5 44 50725.5 45 54005.0 46 57156.5 47 59736.0 48 62784.0 49 65428.5 50 66421.0 51 65112.5 52 62924.0 53 61615.0 54 60777.5 55 58752.0 56 54490.0 57 49454.0 58 45828.5 59 41967.0 60 36076.0 61 30486.0 62 25803.0 63 20311.0 64 15214.5 65 11798.5 66 9196.5 67 7148.5 68 5700.0 69 4549.0 70 3725.0 71 3018.0 72 2347.5 73 1982.5 74 1609.0 75 1228.0 76 958.5 77 651.5 78 428.5 79 288.0 80 202.5 81 141.5 82 94.0 83 86.5 84 75.5 85 52.5 86 41.5 87 38.5 88 33.0 89 25.5 90 29.5 91 24.5 92 13.0 93 15.5 94 17.5 95 11.5 96 7.5 97 7.5 98 8.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025588892564437936 2 0.010341624974228804 3 0.009413530425259553 4 0.01047420990979584 5 0.016042777203611348 6 0.09168248294460535 7 0.057740739439444154 8 0.04865867135310219 9 0.026119232306706076 10-11 0.050514860451040686 12-13 0.012661861346651931 14-15 0.027345642960701164 16-17 0.03397488973905296 18-19 0.0418636934052916 20-21 0.030428242712634748 22-23 0.03745524429768765 24-25 0.030825997519335853 26-27 0.044913146923333425 28-29 0.043885613672688895 30-31 0.026251817242273115 32-33 0.029599586865340775 34-35 0.04434966094717352 36-37 0.00914836055412548 38-39 0.020650103714565847 40-41 0.04120076872745642 42-43 0.02187651436856093 44-45 0.015777607332477274 46-47 0.023235509958123048 48-49 0.02810800634021162 50-51 0.027213058025134126 52-53 0.02422989697487582 54-55 0.02190966060245269 56-57 0.024959114120494515 58-59 0.017070310454255878 60-61 0.02220797670747852 62-63 0.022042245538019724 64-65 0.01982144786727187 66-67 0.027776544001294028 68-69 0.022075391771911482 70-71 0.046603604851813134 72-73 0.02187651436856093 74-75 0.008783751981316132 76-77 0.015346706291884409 78-79 0.02422989697487582 80-81 0.01796525876933337 82-83 0.02386528840206647 84-85 0.011137134587631018 86-87 0.016109069671394867 88-89 0.021810221900777412 90-91 0.019854594101163633 92-93 0.0071927327545117 94-95 0.011170280821522776 96-97 0.014750074081832747 98-99 0.005933175866624858 100-101 0.009247799255800757 102-103 0.00357979326030997 104-105 0.004110133002578114 106-107 8.618020811857336E-4 108-109 0.014120295637889328 110-111 0.0013589955895621183 112-113 0.002220797670747852 114-115 0.012893884983894245 116-117 0.005634859761599028 118-119 0.27441767038987264 120-121 0.0010938257184280465 122-123 9.943870167527695E-4 124-125 0.3086908762339514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1508467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.13486225865164 #Duplication Level Percentage of deduplicated Percentage of total 1 83.64395294828331 55.317813070038554 2 9.07684521915212 12.005918166234519 3 2.83430982298565 5.623400692244979 4 1.3409222676411525 3.5472683788002572 5 0.7841979014854918 2.5931410099133334 6 0.4996369327738277 1.982605183699937 7 0.3564020681586437 1.6499421180459333 8 0.24925089075087384 1.3187338662124215 9 0.1806671268553133 1.075355599431824 >10 0.975339861019767 11.310590396662354 >50 0.04397621571484399 1.9157087217697766 >100 0.014298780508414051 1.5434766165201292 >500 9.998233529433244E-5 0.03848369060279599 >1k 9.998233529433244E-5 0.07756248982316098 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.3146233891758985E-5 16-17 0.0 0.0 0.0 6.629246778351797E-5 1.3258493556703594E-4 18-19 0.0 0.0 0.0 6.629246778351797E-5 1.3258493556703594E-4 20-21 0.0 6.629246778351797E-5 0.0 1.3258493556703594E-4 1.3258493556703594E-4 22-23 0.0 6.629246778351797E-5 0.0 4.3090104059286677E-4 1.3258493556703594E-4 24-25 0.0 6.629246778351797E-5 0.0 0.0011601181862115643 1.3258493556703594E-4 26-27 0.0 6.629246778351797E-5 0.0 0.0020882127351808163 1.3258493556703594E-4 28-29 0.0 6.629246778351797E-5 0.0 0.006065760802191894 1.3258493556703594E-4 30-31 0.0 6.629246778351797E-5 0.0 0.01730233409149819 1.3258493556703594E-4 32-33 0.0 6.629246778351797E-5 0.0 0.03815131520941459 1.3258493556703594E-4 34-35 0.0 6.629246778351797E-5 0.0 0.07129754910117357 1.3258493556703594E-4 36-37 0.0 6.629246778351797E-5 0.0 0.12181240955221426 1.3258493556703594E-4 38-39 0.0 6.629246778351797E-5 0.0 0.2064678909117667 1.6573116945879494E-4 40-41 0.0 6.629246778351797E-5 0.0 0.30315545517402764 1.988774033505539E-4 42-43 0.0 6.629246778351797E-5 0.0 0.397721660467216 1.988774033505539E-4 44-45 0.0 6.629246778351797E-5 0.0 0.5057783829543503 2.3202363724231288E-4 46-47 0.0 6.629246778351797E-5 0.0 0.6285188870555338 2.651698711340719E-4 48-49 0.0 6.629246778351797E-5 0.0 0.7465194797101958 2.651698711340719E-4 50-51 3.3146233891758985E-5 6.629246778351797E-5 0.0 0.8674700871812244 2.651698711340719E-4 52-53 6.629246778351797E-5 9.943870167527696E-5 0.0 0.9952488188339552 3.977548067011078E-4 54-55 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.1368826762534414 3.977548067011078E-4 56-57 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.2903165929383937 4.3090104059286677E-4 58-59 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.456412370969998 4.6404727448462577E-4 60-61 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.6239665832928396 4.6404727448462577E-4 62-63 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.7908247247039544 4.6404727448462577E-4 64-65 6.629246778351797E-5 1.3258493556703594E-4 0.0 1.9650081838051479 4.6404727448462577E-4 66-67 6.629246778351797E-5 1.3258493556703594E-4 0.0 2.172636192903126 4.6404727448462577E-4 68-69 6.629246778351797E-5 1.3258493556703594E-4 0.0 2.4018755464985313 4.971935083763848E-4 70-71 6.629246778351797E-5 1.3258493556703594E-4 0.0 2.631711532303988 5.303397422681438E-4 72-73 6.629246778351797E-5 1.3258493556703594E-4 0.0 2.848388463254417 7.292171456186977E-4 74-75 6.629246778351797E-5 1.3258493556703594E-4 0.0 3.0887649514374527 7.955096134022156E-4 76-77 6.629246778351797E-5 1.3258493556703594E-4 0.0 3.3711377179613473 7.955096134022156E-4 78-79 6.629246778351797E-5 1.3258493556703594E-4 0.0 3.6688571907771266 7.955096134022156E-4 80-81 6.629246778351797E-5 1.3258493556703594E-4 0.0 3.97098511270051 7.955096134022156E-4 82-83 6.629246778351797E-5 1.3258493556703594E-4 0.0 4.282592857516936 7.955096134022156E-4 84-85 6.629246778351797E-5 1.3258493556703594E-4 0.0 4.605669199259911 7.955096134022156E-4 86-87 6.629246778351797E-5 1.3258493556703594E-4 0.0 4.965637299324413 7.955096134022156E-4 88-89 6.629246778351797E-5 1.3258493556703594E-4 0.0 5.367303361624749 7.955096134022156E-4 90-91 6.629246778351797E-5 1.3258493556703594E-4 0.0 5.781167237997252 7.955096134022156E-4 92-93 6.629246778351797E-5 1.3258493556703594E-4 0.0 6.1938047037157595 8.618020811857336E-4 94-95 6.629246778351797E-5 1.3258493556703594E-4 0.0 6.6264956409387805 8.618020811857336E-4 96-97 6.629246778351797E-5 1.3258493556703594E-4 0.0 7.103304215471734 9.943870167527695E-4 98-99 6.629246778351797E-5 1.3258493556703594E-4 0.0 7.63029618811681 9.943870167527695E-4 100-101 6.629246778351797E-5 1.3258493556703594E-4 0.0 8.187815842176196 9.943870167527695E-4 102-103 6.629246778351797E-5 1.3258493556703594E-4 0.0 8.742750089992025 0.0010606794845362875 104-105 9.943870167527696E-5 1.3258493556703594E-4 0.0 9.32151647997603 0.0010606794845362875 106-107 1.3258493556703594E-4 1.3258493556703594E-4 0.0 9.93077740514045 0.0010606794845362875 108-109 1.3258493556703594E-4 1.3258493556703594E-4 0.0 10.58916104893246 0.0010606794845362875 110-111 1.3258493556703594E-4 1.3258493556703594E-4 0.0 11.285729154167774 0.0011269719523198055 112-113 1.3258493556703594E-4 1.3258493556703594E-4 0.0 11.975634866390845 0.0011932644201033235 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 390 0.0 74.76112 2 TACGGTC 185 0.0 54.677303 5 GTACGGT 230 0.0 49.15364 4 AGTACGG 440 0.0 48.68327 3 GTATCAA 3135 0.0 39.487225 1 GGTATCA 2085 0.0 36.25171 1 CTAGTAC 450 0.0 35.70936 1 GTACATG 7680 0.0 34.25247 1 TCAACGC 3605 0.0 33.010696 4 TACATGG 7845 0.0 32.766884 2 ACATGGG 8100 0.0 31.734282 3 CAACGCA 3750 0.0 31.57561 5 CATGGGG 6335 0.0 31.183249 4 ATCAACG 3940 0.0 30.354967 3 GTATAGG 380 0.0 29.757797 1 AACGCAG 4050 0.0 29.380669 6 TATCAAC 4115 0.0 29.209616 2 TATCACG 185 2.86891E-8 28.947767 2 TATTACG 125 4.881507E-5 28.5618 2 GTACGGA 240 4.3655746E-10 27.271646 4 >>END_MODULE