FastQCFastQC Report
Mon 15 Aug 2016
SRR2921639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508467
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.180845852113437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.142064758460079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.10315107987115396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.047.0479661
GTATCAA42850.045.814761
GTATACG1001.0417631E-535.8023531
TCAACGC54850.035.239334
CAACGCA55150.034.6724055
ATCAACG55900.034.1539343
TATCAAC58050.033.7111972
AACGCAG59000.032.827916
GTACATG74350.032.5840761
TACATGG76700.031.19262
CATGGGG60750.029.7547024
ACATGGG79350.029.6245573
ACGCAGA68150.028.157077
CGCAGAG73300.026.2583338
ATGGGGG33600.024.466075
GCAGAGT78950.023.9263119
AGAGTAC72650.023.21236810-11
GAGTACT36150.022.15026712-13
AGTACTT42050.021.52001612-13
TATACGA1400.003106533521.308092
Produced by FastQC (version 0.11.5)