FastQCFastQC Report
Mon 15 Aug 2016
SRR2921638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856771
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.038.7679141
GTACATG121900.037.3099481
GTATCAA46450.037.2942851
TACATGG125900.035.9739842
CATGGGG101250.035.2096254
ACATGGG130450.034.6736153
ATGGGGG67150.034.0323075
TCAACGC55250.030.1616694
CAACGCA56850.029.311215
ATCAACG59400.028.0544153
AACGCAG58900.027.985666
TATCAAC61300.027.6703072
TGGGGGG56100.027.3670466
GTATAGA5700.024.0208261
ACGCAGA69850.023.6760357
CGCAGAG75700.021.9243768
ATGGGGA32050.020.2397275
GTATAGG5300.020.2176931
GAGTACA89500.020.153691
AGTACAT87950.019.759532
Produced by FastQC (version 0.11.5)