##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921638_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856771 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.966116446239198 31.0 31.0 34.0 26.0 34.0 2 30.28359609235603 31.0 31.0 34.0 26.0 34.0 3 30.357887429306036 31.0 31.0 34.0 26.0 34.0 4 33.78122073212044 37.0 35.0 37.0 30.0 37.0 5 33.85862338435919 37.0 35.0 37.0 30.0 37.0 6 33.82427181381011 37.0 35.0 37.0 30.0 37.0 7 33.75438220437523 37.0 35.0 37.0 29.0 37.0 8 33.69936734255328 37.0 35.0 37.0 29.0 37.0 9 35.37564998591641 39.0 37.0 39.0 29.0 39.0 10-11 35.40564022165361 39.0 37.0 39.0 30.0 39.0 12-13 35.332128463876266 39.0 37.0 39.0 29.0 39.0 14-15 36.628555163776255 40.0 38.0 41.0 28.5 41.0 16-17 36.597282863638 40.0 38.0 41.0 28.5 41.0 18-19 36.569137497300424 40.0 38.0 41.0 28.0 41.0 20-21 36.635099050986895 40.0 38.0 41.0 28.5 41.0 22-23 36.58498705548503 40.0 38.0 41.0 28.0 41.0 24-25 36.59098456406309 40.0 38.0 41.0 29.0 41.0 26-27 36.561474462925155 40.0 38.0 41.0 29.0 41.0 28-29 36.50473052411956 40.0 38.0 41.0 29.0 41.0 30-31 36.40548188225688 40.0 37.0 41.0 29.0 41.0 32-33 36.30878121211501 40.0 37.0 41.0 28.5 41.0 34-35 36.17753562501784 40.0 37.0 41.0 27.5 41.0 36-37 36.07240499770839 40.0 37.0 41.0 27.0 41.0 38-39 35.9522730589825 40.0 36.0 41.0 27.0 41.0 40-41 35.8424738430318 40.0 36.0 41.0 26.5 41.0 42-43 35.60921702245457 40.0 35.5 41.0 25.5 41.0 44-45 35.307715921888054 40.0 35.0 41.0 24.5 41.0 46-47 35.07063579730618 39.5 35.0 41.0 24.0 41.0 48-49 34.883302787473525 39.0 35.0 41.0 24.0 41.0 50-51 34.681058407310324 39.0 35.0 41.0 23.0 41.0 52-53 34.48982696304499 39.0 34.5 41.0 23.0 41.0 54-55 34.25321297025859 38.5 34.0 40.0 22.0 41.0 56-57 34.0027690544499 38.0 34.0 40.0 21.0 41.0 58-59 34.00747965150253 38.0 34.0 40.0 22.0 41.0 60-61 33.790282161882104 37.5 34.0 40.0 20.5 41.0 62-63 33.47304783411632 37.0 34.0 40.0 19.5 41.0 64-65 33.17926766413306 36.5 33.5 39.5 18.0 41.0 66-67 32.85087687173055 36.0 33.0 39.0 17.5 41.0 68-69 32.51224841404783 35.5 33.0 39.0 14.5 41.0 70-71 32.14410608524153 35.0 33.0 38.0 12.0 40.0 72-73 31.795738408236666 35.0 32.5 37.0 9.0 39.5 74-75 31.42812818597447 35.0 32.0 37.0 7.0 39.0 76-77 31.130492667108655 35.0 32.0 36.0 6.5 39.0 78-79 30.859073628358047 35.0 32.0 36.0 2.0 37.5 80-81 30.598472563390963 35.0 32.0 35.5 2.0 37.0 82-83 30.371378861475108 35.0 31.5 35.0 2.0 37.0 84-85 30.15563389346344 35.0 31.0 35.0 2.0 36.0 86-87 29.98313443068639 35.0 31.0 35.0 2.0 36.0 88-89 29.81844476243974 35.0 31.0 35.0 2.0 36.0 90-91 29.627919113342465 35.0 31.0 35.0 2.0 35.0 92-93 29.54376737896057 34.5 31.0 35.0 2.0 35.0 94-95 29.414246829576722 34.0 31.0 35.0 2.0 35.0 96-97 29.32390262450243 34.0 30.5 35.0 2.0 35.0 98-99 29.23298268876453 34.0 30.0 35.0 2.0 35.0 100-101 29.122757464436916 34.0 30.0 35.0 2.0 35.0 102-103 28.259474377831193 33.5 28.5 34.5 2.0 35.0 104-105 29.158029719335342 34.0 30.5 35.0 2.0 35.0 106-107 29.416320860246095 35.0 31.0 35.0 2.0 35.0 108-109 29.410501079562316 35.0 31.0 35.0 2.0 35.0 110-111 29.350044512758977 35.0 31.0 35.0 2.0 35.0 112-113 29.26496428477179 35.0 31.0 35.0 2.0 35.0 114-115 29.14551363630733 35.0 31.0 35.0 2.0 35.0 116-117 29.0244704381962 35.0 31.0 35.0 2.0 35.0 118-119 28.73885417210846 35.0 30.5 35.0 2.0 35.0 120-121 28.607670520489606 35.0 30.0 35.0 2.0 35.0 122-123 28.369721683503244 34.0 30.0 35.0 2.0 35.0 124-125 27.60473612524108 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71921.0 3 12952.0 4 6538.0 5 5738.0 6 6224.0 7 6791.0 8 6660.0 9 6916.0 10 7702.0 11 8203.0 12 8250.0 13 8442.0 14 8624.0 15 7761.0 16 6425.0 17 5926.0 18 5352.0 19 5145.0 20 4858.0 21 5148.0 22 5848.0 23 8152.0 24 12405.0 25 16212.0 26 16840.0 27 17538.0 28 20766.0 29 25202.0 30 30285.0 31 37524.0 32 49510.0 33 67969.0 34 107839.0 35 176688.0 36 346996.0 37 555495.0 38 155799.0 39 127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28552335218632 17.6664459759316 12.131059051561364 24.916971620320712 2 16.936141516578385 19.902142333136197 37.71581418246728 25.44590196781814 3 19.1986616532525 24.71255545698929 28.617987858575184 27.470795031183027 4 13.477802805795152 15.67182990715216 35.005542431107415 35.84482485594527 5 14.408597401086364 38.286520424806675 32.164249531906826 15.140632642200135 6 33.29987271667681 34.16163634275819 17.7905534317708 14.747937508794193 7 29.159573338679262 33.219678414241734 19.82693626971514 17.793811977363863 8 27.780193607660248 34.267370400605664 19.183485422846566 18.768950568887526 9 28.450887236709764 15.579692382946654 18.265940981614197 37.70347939872939 10-11 26.634902638691905 25.841619211022067 26.34790555716016 21.175572593125867 12-13 26.18275502572416 23.568271557025806 26.446204424560204 23.80276899268983 14-15 24.27387065701547 24.655804401652397 23.815598649292212 27.254726292039916 16-17 24.6758529434266 27.18150726630325 24.484214768369412 23.658425021900737 18-19 24.597155159617156 26.751569877850045 25.652998871471393 22.998276091061403 20-21 25.610831280056694 25.547313716157298 25.38197410325887 23.459880900527132 22-23 25.636783742042475 24.97561322534996 25.18334194043027 24.204261092177294 24-25 25.06054214514491 25.28229206231194 25.155712511684165 24.501453280858986 26-27 24.480120136721798 25.506234086100676 25.517414732751565 24.49623104442596 28-29 24.687979722925242 25.96235550725286 25.32360502163653 24.02605974818537 30-31 25.440697012924886 25.450932625962686 25.295566794378388 23.812803566734043 32-33 24.972099980524533 25.503782900540706 25.225146355569215 24.298970763365542 34-35 24.904371356506925 25.89349940755772 25.344540613413496 23.85758862252186 36-37 24.93976159947732 25.777807222575472 25.31992266431611 23.9625085136311 38-39 24.92571435959206 25.958063897501976 25.195814577870095 23.92040716503587 40-41 24.899868556854482 25.590355914847258 25.421384525485934 24.08839100281233 42-43 24.698053823870456 25.611090685531824 25.182988500174403 24.50786699042332 44-45 24.533996462604307 25.620451501400115 25.47778677643169 24.367765259563885 46-47 24.371355645866824 25.59341290397652 25.452056137598746 24.583175312557913 48-49 24.402221520671734 25.819305615863758 25.476024751563138 24.302448111901374 50-51 24.444206805116416 25.84667989425828 25.556304987391048 24.152808313234253 52-53 24.58944330103505 25.691695346175614 25.35484074286864 24.3640206099207 54-55 24.256970651603304 25.43746782978194 25.69555681311012 24.610004705504632 56-57 24.429220423460507 25.664434779938556 25.16502116871566 24.74132362788528 58-59 24.39975275024947 25.843928308345752 25.457378371572204 24.298940569832567 60-61 24.860025593626965 25.755479317564333 25.49992013775631 23.88457495105239 62-63 24.559077666974083 25.724841838977426 25.51682454038493 24.199255953663563 64-65 24.319720745325554 25.40571114594612 25.57138393746936 24.70318417125896 66-67 24.450736822370697 25.593088943408464 25.345946103197154 24.610228131023685 68-69 24.228720109032338 25.54429977428582 25.558871536849594 24.66810857983225 70-71 24.155341009051096 25.530746651471492 25.739545572721973 24.57436676675544 72-73 24.25241786973199 26.097047703417676 25.28951544023989 24.361018986610443 74-75 24.12749103125065 25.845698999588222 25.448922228467257 24.57788774069387 76-77 24.094176063139557 25.37637513136568 25.507565747479475 25.021883058015288 78-79 24.040037009453034 25.572077525915372 25.660614696308347 24.727270768323248 80-81 24.212978150686517 25.712495764645173 25.47073713219748 24.603788952470833 82-83 24.308394456973996 25.814041373640862 25.496255006993835 24.38130916239131 84-85 24.200116722725355 25.882362129696883 25.243376577837278 24.67414456974048 86-87 23.9245527144976 26.034333836893403 25.410912287628772 24.630201160980224 88-89 23.869448788442135 25.63125932956927 25.623475149071094 24.875816732917492 90-91 24.34559380636876 25.348201869412616 25.660345240426352 24.645859083792274 92-93 24.357574998619803 25.475764394119953 25.62224044532598 24.54442016193427 94-95 24.225317746370084 25.45280860778707 25.565679163761413 24.75619448208143 96-97 23.999951521288885 25.44768743081684 25.583912609043434 24.96844843885084 98-99 23.760883873393205 25.470741326025532 25.483667861293966 25.284706939287293 100-101 23.745991614134944 25.831873881846505 25.58491395318211 24.837220550836445 102-103 23.676045179300164 25.632859748875823 25.744860076340114 24.946234995483902 104-105 23.76040157484556 25.70194809041897 25.55289251302048 24.984757821714986 106-107 23.731706338445562 25.528684025072756 25.77467750887098 24.9649321276107 108-109 23.739652811163044 25.500617431082706 25.75181829077301 25.00791146698124 110-111 23.43718653047452 26.15423434466709 25.662700664255812 24.745878460602576 112-113 23.33727038149512 26.188183361959066 25.692174537800778 24.782371718745036 114-115 23.434716296747364 26.273970735927143 25.55124309961414 24.74006986771135 116-117 23.353683496170543 26.108091950060864 25.56087274999192 24.977351803776674 118-119 23.22845086230037 25.744571566528997 25.86371814003881 25.163259431131824 120-121 23.22775296444923 25.95420607910691 25.966593294853972 24.85144766158988 122-123 23.15284546233764 26.082985122696616 25.79479408828685 24.969375326678893 124-125 22.954575108928594 25.992473263227506 25.815745418974927 25.237206208868972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 632.0 1 527.0 2 368.0 3 288.5 4 247.0 5 212.0 6 202.0 7 242.5 8 303.5 9 336.0 10 354.0 11 374.5 12 401.5 13 447.0 14 521.5 15 595.0 16 612.0 17 668.5 18 775.5 19 863.0 20 907.5 21 1028.0 22 1247.5 23 1483.5 24 1804.0 25 2402.5 26 3592.0 27 4981.5 28 6105.5 29 7612.0 30 9937.0 31 12552.0 32 15439.0 33 18549.5 34 21927.5 35 25123.0 36 28684.5 37 32557.5 38 35288.5 39 38231.0 40 41566.5 41 44542.5 42 47392.5 43 51392.0 44 55598.5 45 59386.5 46 63978.5 47 68635.5 48 74164.5 49 79818.0 50 83837.0 51 84946.0 52 83207.5 53 82120.5 54 81084.5 55 78111.5 56 74049.0 57 68583.0 58 62071.0 59 54248.5 60 46463.5 61 40293.0 62 34165.0 63 26886.5 64 20142.5 65 15443.0 66 11939.5 67 9348.5 68 7506.5 69 5966.5 70 4810.0 71 3875.0 72 3012.0 73 2555.0 74 2051.0 75 1532.5 76 1163.5 77 820.0 78 535.5 79 339.0 80 237.0 81 160.0 82 119.0 83 97.0 84 75.0 85 58.0 86 56.5 87 42.0 88 32.0 89 36.5 90 35.0 91 26.0 92 17.5 93 15.0 94 17.0 95 15.5 96 12.0 97 9.0 98 7.0 99 6.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025743616202536554 2 0.01060981671945544 3 0.008186254524655975 4 0.009963533467508917 5 0.01631865211164974 6 0.09985076242573802 7 0.06215090606219076 8 0.05148723240507311 9 0.0270361827064296 10-11 0.054018508475197 12-13 0.013329592071397067 14-15 0.030106028153175594 16-17 0.03603029129601873 18-19 0.04386647572587034 20-21 0.03185637862719743 22-23 0.040096490089515616 24-25 0.03371444297654369 26-27 0.04769031829988728 28-29 0.04750181901806954 30-31 0.027305467394740657 32-33 0.03174866475187301 34-35 0.04814810227001607 36-37 0.010205889686988864 38-39 0.022781484631114984 40-41 0.044997471416776756 42-43 0.023616267164879245 44-45 0.01726114852073842 46-47 0.025582045389549925 48-49 0.030429169779148852 50-51 0.029621315714215703 52-53 0.02587825854669208 54-55 0.023400839414230403 56-57 0.026605327205131923 58-59 0.01876914277528031 60-61 0.02420869347916356 62-63 0.023427767883061508 64-65 0.021596632002546357 66-67 0.02999831427785117 68-69 0.02375090950903477 70-71 0.04946759724274021 72-73 0.023508553289554826 74-75 0.009990461936340024 76-77 0.016803364550609632 78-79 0.026309114047989763 80-81 0.020142494685666677 82-83 0.026524541798638605 84-85 0.012629451881788331 86-87 0.01736886239606284 88-89 0.02380476644669698 90-91 0.022215986785661775 92-93 0.00826703993114929 94-95 0.011821597816855175 96-97 0.015403084171392164 98-99 0.0067321172077762954 100-101 0.010475174375299916 102-103 0.004308555012976829 104-105 0.00436241195063904 106-107 7.809255961020502E-4 108-109 0.01553772651554769 110-111 0.0016964935363596265 112-113 0.002692846883110518 114-115 0.014137446136330221 116-117 0.005978120080505351 118-119 0.2838260614798486 120-121 9.694248779197865E-4 122-123 8.886394714264711E-4 124-125 0.3178905745511967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1856771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.579930364175496 #Duplication Level Percentage of deduplicated Percentage of total 1 81.52762149846477 51.835204976292474 2 10.463719447480056 13.305651076421018 3 3.2477909926486053 6.1948297544998425 4 1.4633581273016747 3.721608313267629 5 0.8341150523952416 2.6516488473500033 6 0.5307504443190615 2.024704577433668 7 0.3361662229917506 1.4961397534022376 8 0.26282819996259527 1.336847892109072 9 0.1906675725494242 1.0910367886858894 >10 1.0733003535638745 12.082719992141607 >50 0.051653356148417186 2.174867709967698 >100 0.017351961146101807 1.7040945419946405 >500 4.2298189263360907E-4 0.17141699724404 >1k 2.537891355801643E-4 0.20922877919024577 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.385693766221036E-5 0.0 3 0.0 0.0 0.0 5.385693766221036E-5 0.0 4 0.0 0.0 0.0 5.385693766221036E-5 0.0 5 0.0 0.0 0.0 5.385693766221036E-5 0.0 6 0.0 0.0 0.0 5.385693766221036E-5 0.0 7 0.0 0.0 0.0 5.385693766221036E-5 0.0 8 0.0 0.0 0.0 5.385693766221036E-5 5.385693766221036E-5 9 0.0 0.0 0.0 5.385693766221036E-5 5.385693766221036E-5 10-11 0.0 0.0 0.0 1.0771387532442072E-4 5.385693766221036E-5 12-13 0.0 0.0 0.0 1.615708129866311E-4 5.385693766221036E-5 14-15 0.0 0.0 0.0 1.615708129866311E-4 8.078540649331554E-5 16-17 0.0 0.0 0.0 2.4235621947994664E-4 1.0771387532442072E-4 18-19 0.0 0.0 0.0 2.692846883110518E-4 2.1542775064884145E-4 20-21 0.0 0.0 0.0 3.231416259732622E-4 2.692846883110518E-4 22-23 0.0 0.0 0.0 3.500700948043674E-4 3.500700948043674E-4 24-25 0.0 0.0 0.0 5.654978454532088E-4 3.769985636354726E-4 26-27 0.0 0.0 0.0 8.617110025953658E-4 4.8471243895989327E-4 28-29 0.0 0.0 0.0 0.0029890600402526753 4.8471243895989327E-4 30-31 0.0 0.0 0.0 0.012144739442828437 4.8471243895989327E-4 32-33 0.0 0.0 0.0 0.03357980063238816 5.385693766221036E-4 34-35 0.0 0.0 0.0 0.059296488366093615 5.92426314284314E-4 36-37 0.0 0.0 0.0 0.09492285262964575 5.92426314284314E-4 38-39 0.0 0.0 0.0 0.15465019649703707 6.462832519465244E-4 40-41 5.385693766221036E-5 0.0 0.0 0.22617220971245244 6.462832519465244E-4 42-43 5.385693766221036E-5 0.0 0.0 0.2947051628876151 6.462832519465244E-4 44-45 5.385693766221036E-5 0.0 0.0 0.37029337489652736 6.462832519465244E-4 46-47 8.078540649331554E-5 0.0 0.0 0.4510518529210118 6.462832519465244E-4 48-49 1.0771387532442072E-4 0.0 0.0 0.5406428687220988 6.462832519465244E-4 50-51 1.0771387532442072E-4 0.0 0.0 0.6341115840348648 6.462832519465244E-4 52-53 1.0771387532442072E-4 0.0 0.0 0.7291152220710039 8.347825337642607E-4 54-55 1.0771387532442072E-4 0.0 0.0 0.8388218040889264 9.424964090886814E-4 56-57 1.0771387532442072E-4 0.0 0.0 0.9524330140873591 9.694248779197865E-4 58-59 1.0771387532442072E-4 0.0 0.0 1.078862175249398 9.694248779197865E-4 60-61 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.2061799758828633 9.963533467508916E-4 62-63 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.3543942683292662 0.0011309956909064177 64-65 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.4896559672679075 0.0011309956909064177 66-67 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.6430674541987136 0.001157924159737523 68-69 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.8204183499203725 0.001184852628568628 70-71 1.0771387532442072E-4 5.385693766221036E-5 0.0 1.9998158092731955 0.0012117810973997331 72-73 1.0771387532442072E-4 5.385693766221036E-5 0.0 2.170273016974091 0.0012925665038930488 74-75 1.0771387532442072E-4 5.385693766221036E-5 0.0 2.3521209669905447 0.0012925665038930488 76-77 1.0771387532442072E-4 5.385693766221036E-5 0.0 2.56423651597316 0.0013194949727241539 78-79 1.0771387532442072E-4 5.385693766221036E-5 0.0 2.799887546714161 0.0013464234415552592 80-81 1.0771387532442072E-4 5.385693766221036E-5 0.0 3.0295066004369953 0.0013733519103863645 82-83 1.0771387532442072E-4 5.385693766221036E-5 0.0 3.2711357512585018 0.0014810657857107852 84-85 1.0771387532442072E-4 5.385693766221036E-5 0.0 3.523778645831931 0.0015079942545418903 86-87 1.0771387532442072E-4 5.385693766221036E-5 0.0 3.806473711620873 0.0015349227233729954 88-89 1.0771387532442072E-4 5.385693766221036E-5 0.0 4.120540443598053 0.0015618511922041005 90-91 1.0771387532442072E-4 8.078540649331554E-5 0.0 4.452137608784282 0.0015618511922041005 92-93 1.0771387532442072E-4 1.0771387532442072E-4 0.0 4.7705398242432695 0.0016157081298663108 94-95 1.0771387532442072E-4 1.0771387532442072E-4 0.0 5.093223666246403 0.0016157081298663108 96-97 1.0771387532442072E-4 1.0771387532442072E-4 0.0 5.464836536115655 0.001804207411684047 98-99 1.615708129866311E-4 1.0771387532442072E-4 0.0 5.8721834841237825 0.0018849928181773627 100-101 1.615708129866311E-4 1.0771387532442072E-4 0.0 6.313352588983779 0.0018849928181773627 102-103 1.615708129866311E-4 1.0771387532442072E-4 0.0 6.764269799560635 0.0019927066935017832 104-105 1.615708129866311E-4 1.0771387532442072E-4 0.0 7.231748018468621 0.0021273490376573093 106-107 1.615708129866311E-4 1.0771387532442072E-4 0.0 7.738487944932358 0.0022081344441506248 108-109 1.615708129866311E-4 1.0771387532442072E-4 0.0 8.279965596188221 0.0022619913818128353 110-111 1.615708129866311E-4 1.0771387532442072E-4 0.0 8.851818560285572 0.00228891985064394 112-113 1.615708129866311E-4 1.0771387532442072E-4 0.0 9.422782884911495 0.0023158483194750455 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3240 0.0 38.767914 1 GTACATG 12190 0.0 37.309948 1 GTATCAA 4645 0.0 37.294285 1 TACATGG 12590 0.0 35.973984 2 CATGGGG 10125 0.0 35.209625 4 ACATGGG 13045 0.0 34.673615 3 ATGGGGG 6715 0.0 34.032307 5 TCAACGC 5525 0.0 30.161669 4 CAACGCA 5685 0.0 29.31121 5 ATCAACG 5940 0.0 28.054415 3 AACGCAG 5890 0.0 27.98566 6 TATCAAC 6130 0.0 27.670307 2 TGGGGGG 5610 0.0 27.367046 6 GTATAGA 570 0.0 24.020826 1 ACGCAGA 6985 0.0 23.676035 7 CGCAGAG 7570 0.0 21.924376 8 ATGGGGA 3205 0.0 20.239727 5 GTATAGG 530 0.0 20.217693 1 GAGTACA 8950 0.0 20.15369 1 AGTACAT 8795 0.0 19.75953 2 >>END_MODULE