FastQCFastQC Report
Mon 15 Aug 2016
SRR2921638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856771
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.2074030669371721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.18919942200734502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.13173406952176656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.052.7903251
GTATCAA63200.043.4205931
CATGGGG96450.036.9086154
GTACATG120000.036.489661
TACATGG125650.034.695182
ACATGGG125500.034.4496733
ATGGGGG65700.033.1900635
TCAACGC83700.032.5571484
CAACGCA85950.031.7476775
AACGCAG88500.031.045766
ATCAACG87600.030.9740583
TATCAAC88400.030.8977952
TGGGGGG48900.027.1794516
ACGCAGA103750.026.251217
CGCAGAG110050.024.9636848
AGAGTAC110650.023.48251310-11
GCAGAGT118850.022.9153529
AGTACTT58250.022.84478212-13
ATGGGGA29950.022.6777735
GAGTACT54450.022.2521512-13
Produced by FastQC (version 0.11.5)