FastQCFastQC Report
Mon 15 Aug 2016
SRR2921637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621269
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31100.042.5057641
GGTATCA20150.040.4856151
GTACATG85200.038.9287831
TACATGG87100.037.3866962
TCAACGC37200.036.005934
ACATGGG92500.035.138683
CAACGCA38550.034.7385945
CATGGGG79900.033.8254554
ATGGGGG48950.033.6807025
AACGCAG41850.032.7053956
ATCAACG41050.032.1939543
TATCAAC41250.031.7502142
AGCCTCG10050.031.3947982
ACGCAGA49900.027.3024317
CGCAGAG52050.026.1722458
TATCACG2302.3528264E-723.2949922
TGGGGGG39700.023.2342286
GCAGAGT59950.022.425679
AGAGTAC53650.021.0106210-11
ATGGGGA30550.019.8720655
Produced by FastQC (version 0.11.5)