FastQCFastQC Report
Mon 15 Aug 2016
SRR2921637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621269
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.1482789099156278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.12761608345067968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.056.558761
GTATCAA42900.050.1933981
TATCACG1900.040.8080752
TCAACGC54600.039.2070474
TATCAAC56550.037.9687462
CAACGCA56250.037.914635
GTACATG86500.037.374881
ATCAACG57700.037.107493
AACGCAG58550.036.444386
TACATGG89200.035.772172
ACATGGG91050.034.651233
ATGGGGG49000.033.5551645
CATGGGG80850.033.4103134
ACGCAGA70450.030.2010047
CGCAGAG74150.028.8529768
TGGGGGG36650.027.6470876
GCAGAGT79500.026.6864139
GTGGTAT13450.023.5044571
AGAGTAC75600.023.09687210-11
GAGTACT37450.022.4976112-13
Produced by FastQC (version 0.11.5)