##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1621269
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.659137996223947	33.0	31.0	34.0	30.0	34.0
2	31.831210613414555	33.0	31.0	34.0	30.0	34.0
3	31.89707321857138	34.0	31.0	34.0	30.0	34.0
4	35.29409370067521	37.0	35.0	37.0	33.0	37.0
5	35.15071342263375	37.0	35.0	37.0	33.0	37.0
6	35.18205245397278	37.0	35.0	37.0	33.0	37.0
7	35.06760630099015	37.0	35.0	37.0	33.0	37.0
8	35.094653632432376	37.0	35.0	37.0	33.0	37.0
9	36.790725659961424	39.0	38.0	39.0	33.0	39.0
10-11	36.74291465512509	39.0	38.0	39.0	33.0	39.0
12-13	36.6705003919769	39.0	38.0	39.0	33.0	39.0
14-15	38.08646313474198	41.0	39.0	41.0	33.0	41.0
16-17	38.09620951242514	41.0	39.0	41.0	33.0	41.0
18-19	38.10803019116507	41.0	39.0	41.0	33.5	41.0
20-21	38.04708287150374	41.0	39.0	41.0	33.0	41.0
22-23	38.05745653559033	41.0	39.0	41.0	33.0	41.0
24-25	38.02291877535437	41.0	39.0	41.0	33.0	41.0
26-27	37.951017382063064	41.0	39.0	41.0	33.0	41.0
28-29	37.87751292351855	41.0	38.0	41.0	33.0	41.0
30-31	37.756534233369045	41.0	38.0	41.0	33.0	41.0
32-33	37.62801299475905	41.0	38.0	41.0	33.0	41.0
34-35	37.49496042914532	40.5	38.0	41.0	32.0	41.0
36-37	37.41825816690506	40.0	38.0	41.0	32.0	41.0
38-39	37.333308661301736	40.0	38.0	41.0	32.0	41.0
40-41	37.212347549974744	40.0	38.0	41.0	31.5	41.0
42-43	37.04464805038522	40.0	37.5	41.0	31.0	41.0
44-45	36.885557856222505	40.0	37.0	41.0	31.0	41.0
46-47	36.67682568407834	40.0	37.0	41.0	30.0	41.0
48-49	36.47670929376926	40.0	36.5	41.0	30.0	41.0
50-51	36.22798005759686	40.0	36.0	41.0	29.0	41.0
52-53	36.10029643445967	40.0	35.5	41.0	29.0	41.0
54-55	35.971537110744734	40.0	35.0	41.0	29.0	41.0
56-57	35.77689081824176	39.0	35.0	41.0	28.0	41.0
58-59	35.58029913604713	39.0	35.0	41.0	28.0	41.0
60-61	35.32565940630457	39.0	35.0	41.0	28.0	41.0
62-63	34.968663127463735	37.5	35.0	40.5	28.0	41.0
64-65	34.681621001820176	37.0	35.0	40.0	28.0	41.0
66-67	34.37475088958094	36.5	35.0	39.5	28.0	41.0
68-69	34.001993500153276	36.0	35.0	39.0	26.5	41.0
70-71	33.62933017284608	35.5	34.0	39.0	26.0	41.0
72-73	33.309125135927474	35.0	34.0	37.5	26.0	40.0
74-75	32.98687509598962	35.0	34.0	37.0	26.0	39.0
76-77	32.66247180449389	35.0	34.0	36.5	26.0	39.0
78-79	32.404088710756824	35.0	34.0	36.0	26.0	38.0
80-81	32.15593710852425	35.0	34.0	36.0	25.0	37.0
82-83	31.93795878413761	35.0	34.0	35.0	25.0	37.0
84-85	31.73144832843902	35.0	34.0	35.0	24.5	36.0
86-87	31.6033782796069	35.0	33.0	35.0	24.0	36.0
88-89	31.511112283032613	35.0	33.0	35.0	24.0	36.0
90-91	31.40953968773843	35.0	33.0	35.0	24.0	35.5
92-93	31.33303696055374	35.0	33.0	35.0	24.0	35.0
94-95	31.26986329844091	35.0	33.0	35.0	23.0	35.0
96-97	31.20411264262747	35.0	33.0	35.0	23.0	35.0
98-99	31.125161524706883	35.0	33.0	35.0	21.5	35.0
100-101	31.05810911082615	35.0	33.0	35.0	20.0	35.0
102-103	30.37205454492746	34.0	31.5	35.0	19.5	35.0
104-105	30.891772740982525	35.0	33.0	35.0	20.0	35.0
106-107	30.98702528698199	35.0	33.0	35.0	20.0	35.0
108-109	30.974801837326194	35.0	33.0	35.0	19.5	35.0
110-111	30.909453027227435	35.0	33.0	35.0	19.0	35.0
112-113	30.87008047399907	35.0	33.0	35.0	19.0	35.0
114-115	30.746956242301557	35.0	33.0	35.0	18.0	35.0
116-117	30.933329077407883	35.0	33.0	35.0	19.0	35.0
118-119	30.920950502353403	35.0	33.0	35.0	17.5	35.0
120-121	30.839293479367086	35.0	33.0	35.0	13.0	35.0
122-123	30.70897241605187	35.0	33.0	35.0	2.0	35.0
124-125	30.07472572410871	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28569.0
3	7518.0
4	3970.0
5	3138.0
6	3328.0
7	3680.0
8	4117.0
9	4316.0
10	4749.0
11	5107.0
12	5689.0
13	7254.0
14	9719.0
15	8734.0
16	5014.0
17	4349.0
18	3990.0
19	3576.0
20	3426.0
21	3456.0
22	3840.0
23	4777.0
24	6130.0
25	8441.0
26	9909.0
27	11484.0
28	13813.0
29	16861.0
30	20029.0
31	25772.0
32	33254.0
33	44893.0
34	70331.0
35	124040.0
36	287128.0
37	603986.0
38	212535.0
39	347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22069288880526	17.396376693123244	11.038346098914488	27.34458431915701
2	16.17270076090282	19.609038064879055	37.19350469272751	27.02475648149062
3	19.115910428643883	23.59472502847384	28.30601942514311	28.983345117739173
4	13.584247504615131	15.122996092890842	33.742805457596376	37.54995094489765
5	14.806385580374384	37.07972474585139	32.20256375771021	15.911325916064017
6	34.500369424604976	33.459005596613025	16.758482723458677	15.282142255323325
7	30.408216288537766	31.49924925527234	19.239183629136424	18.853350827053468
8	28.461790989883585	34.13393362928516	18.44361179709898	18.960663583732277
9	28.442164794964203	15.341975187525028	18.2407539379176	37.97510607959317
10-11	27.195568915382246	24.818202774149288	25.982106184700736	22.00412212576773
12-13	27.071822181517685	22.2797681240276	26.531537341646764	24.116872352807945
14-15	24.55497949287489	23.416911967329316	24.07802798325362	27.95008055654218
16-17	24.60574276862086	26.50923016587361	24.653353867098076	24.23167319840745
18-19	24.602472121912356	25.550288537628667	26.585537793724516	23.261701546734457
20-21	25.303901557087716	24.626799602807985	26.330042628118022	23.73925621198628
22-23	25.111991450869485	24.171587094267117	26.27984832386771	24.436573130995686
24-25	24.517070390494936	24.52946862168062	26.334367341702038	24.619093646122405
26-27	24.25139432548695	24.73889936800447	26.523858047117777	24.485848259390803
28-29	24.366254522127196	25.306456040143228	26.2522707015504	24.075018736179178
30-31	25.037548535530014	24.748972234130154	26.263835902876508	23.94964332746332
32-33	24.639884869258076	24.628565489091294	26.321815221451182	24.40973442019945
34-35	24.674545226879218	24.985643288615236	26.22389807999418	24.115913404511367
36-37	24.640669686903113	25.008180792270796	26.27926464469763	24.071884876128465
38-39	24.67185449090568	25.0799083659834	26.25774719472599	23.990489948384933
40-41	24.724712468557513	24.781552589602608	26.316019970219845	24.17771497162003
42-43	24.621016677332552	24.805631587469254	25.996499533528144	24.576852201670047
44-45	24.34034852026003	25.00316124443852	26.142504581491334	24.513985653810117
46-47	24.431129857216526	24.975080126030413	25.869728274681158	24.7240617420719
48-49	24.356995416769855	25.281043203752198	25.78501039175962	24.576950987718323
50-51	24.43956685166841	25.293200414989457	25.833833977076893	24.43339875626524
52-53	24.623170129569335	25.22717733262894	25.5620104295714	24.58764210823032
54-55	24.367431671270797	24.854049976298253	25.95586509526807	24.822653257162877
56-57	24.450249266387356	25.12734713640492	25.650212874882616	24.772190722325107
58-59	24.383811830072695	25.262419676572144	25.818561882192714	24.535206611162444
60-61	24.658793197297793	25.137030132288473	25.962587913468937	24.2415887569448
62-63	24.63050161233417	25.152166350450145	25.93668350139275	24.280648535822934
64-65	24.482619627779112	24.811436230334458	25.854693408278766	24.851250733607667
66-67	24.37731403308306	25.16918559913892	25.692309352972835	24.76119101480519
68-69	24.28280252655576	25.09164948094335	25.870118799685553	24.755429192815335
70-71	24.213146746867267	24.94803450139027	26.096364080460475	24.742454671281987
72-73	24.344841772961956	25.348493011943706	25.58427071289369	24.722394502200643
74-75	24.276191795025845	25.300926875440066	25.58528422936227	24.83759710017182
76-77	24.268954627322298	24.93116380054773	25.705262638474252	25.09461893365572
78-79	24.14704055531877	25.007386198571858	25.862519868719957	24.98305337738941
80-81	24.291342152351028	25.268477963866577	25.497372737035	24.94280714674739
82-83	24.353169439675153	25.31061221254588	25.581862991678662	24.754355356100305
84-85	24.353466155412377	25.29046412887901	25.432824165924693	24.923245549783914
86-87	24.138498916589413	25.42471360397318	25.457249845645602	24.9795376337918
88-89	24.013290995578714	25.164872986736142	25.580848827569874	25.24098719011527
90-91	24.189788365026306	25.069722070848048	25.71724373380309	25.023245830322555
92-93	24.168999715654834	25.23560248175966	25.727562791862425	24.867835010723084
94-95	24.176069547495935	24.96069325441349	25.637250396768362	25.225986801322215
96-97	23.965162575674867	25.048219115556254	25.647881720529593	25.338736588239286
98-99	23.867413947145316	25.104509989628415	25.530414218848158	25.49766184437811
100-101	23.802620046395756	25.15794726229885	25.618142331716697	25.421290359588696
102-103	23.657270624518116	25.015605242868155	25.798982266769464	25.528141865844255
104-105	23.89677468698902	25.11911965256845	25.592390898734262	25.39171476170827
106-107	23.894725057894803	25.028966676833658	25.628377196864538	25.447931068406998
108-109	23.89969480000395	24.95074733289252	25.618572789088788	25.530985078014744
110-111	23.754678436877803	25.30192417293131	25.538837502498048	25.40455988769284
112-113	23.660975738381985	25.352217358734492	25.59332545676538	25.393481446118138
114-115	23.623809497375202	25.329356201839424	25.596091703474254	25.45074259731112
116-117	23.560426437969856	25.146615808601297	25.63590058004841	25.657057173380444
118-119	23.61489510131499	25.117518724968868	25.54312124552269	25.724464928193452
120-121	23.466353076731153	25.15461297641815	25.73318764107769	25.645846305773006
122-123	23.381519388891476	25.270740086124878	25.53895897025167	25.80878155473198
124-125	23.190994210687503	25.18625705140238	25.57806680431926	26.04468193359086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	28.5
2	33.5
3	69.0
4	68.0
5	55.0
6	129.0
7	342.0
8	564.0
9	643.0
10	588.0
11	512.5
12	527.0
13	580.5
14	663.0
15	757.5
16	782.0
17	824.5
18	901.5
19	944.0
20	1009.5
21	1126.5
22	1242.5
23	1382.5
24	1552.0
25	1897.0
26	2443.5
27	3198.0
28	4035.5
29	5189.5
30	6678.5
31	8359.0
32	10783.5
33	12967.5
34	15174.0
35	17846.5
36	20622.5
37	23587.5
38	26734.0
39	30329.5
40	33760.0
41	36712.0
42	40111.0
43	45073.5
44	50207.0
45	54979.5
46	59327.0
47	63628.0
48	68664.5
49	73062.0
50	75635.5
51	76260.0
52	75574.0
53	74855.5
54	74644.0
55	72167.5
56	67386.0
57	62778.0
58	57976.5
59	51318.0
60	43017.0
61	35937.0
62	29808.0
63	22717.5
64	16576.5
65	12550.5
66	9618.5
67	7528.0
68	5917.5
69	4523.0
70	3509.5
71	2695.5
72	2056.0
73	1665.5
74	1272.5
75	925.5
76	651.0
77	399.5
78	244.0
79	141.5
80	84.5
81	48.0
82	22.5
83	13.5
84	9.0
85	8.0
86	6.0
87	2.5
88	3.5
89	2.5
90	1.0
91	1.5
92	0.5
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0230683495459421
2	0.0022821629229942716
3	0.029791478156925223
4	0.16536429179858494
5	0.02263658899294318
6	0.072905853377817
7	0.05415510936186407
8	0.04533485806488621
9	0.03404740360791454
10-11	0.06097075809134696
12-13	0.05674567267985757
14-15	0.060353957301348515
16-17	0.05233554703136864
18-19	0.03679216712340765
20-21	0.024086070849439544
22-23	0.0031765240684920267
24-25	0.004317605529989162
26-27	0.0033924043449914854
28-29	0.004564325845988543
30-31	0.002097122685994736
32-33	0.009591252284475925
34-35	0.004687686003988234
36-37	0.005767087386485525
38-39	0.0022821629229942716
40-41	0.0033924043449914854
42-43	0.003484924463491253
44-45	0.004440965687988853
46-47	0.004502645766988699
48-49	0.0020046025674949684
50-51	0.0014186418169964393
52-53	8.326810664979099E-4
54-55	0.004965246359487537
56-57	7.093209084982196E-4
58-59	6.78480868998297E-4
60-61	9.252011849976778E-5
62-63	0.001048561342997368
64-65	3.084003949992259E-5
66-67	0.002868123673492801
68-69	6.476408294983744E-4
70-71	6.168007899984518E-5
72-73	0.00237468304149404
74-75	0.00428676549048924
76-77	0.0017887222909955102
78-79	1.2336015799969036E-4
80-81	0.0
82-83	0.004656845964488312
84-85	0.0016962021724957427
86-87	0.0
88-89	0.0012952816589967488
90-91	6.476408294983744E-4
92-93	0.0
94-95	0.0028372836339928785
96-97	0.0014803218959962845
98-99	7.710009874980647E-4
100-101	0.0
102-103	0.0011719215009970585
104-105	0.0
106-107	5.859607504985293E-4
108-109	0.0022821629229942716
110-111	6.168007899984518E-5
112-113	2.775603554993033E-4
114-115	0.0
116-117	0.0015420019749961295
118-119	0.002343843001994117
120-121	0.0026830834364932653
122-123	0.0011410814614971358
124-125	0.10689157690673169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1621269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.20069319122794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31163277975241	50.57639923411173
2	10.31037953401928	12.826255081612981
3	3.18074451846663	5.935335417384688
4	1.568824118861865	3.9032779075330164
5	0.8975138021773764	2.791299032206372
6	0.5648866358861548	2.1081804195947766
7	0.43434500285513095	1.8911592183214518
8	0.3028203261401059	1.5068507358646648
9	0.21059155429031792	1.17890465913683
>10	1.1450816586403416	12.51433150431611
>50	0.053398659233797226	2.269159158222951
>100	0.019085664335606205	1.9447932025317034
>500	3.9756876626095E-4	0.1941230648930071
>1k	2.981765746957125E-4	0.359931364269773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2404	0.1482789099156278	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2069	0.12761608345067968	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2336015799969036E-4
2	0.0	0.0	0.0	0.0	1.2336015799969036E-4
3	0.0	0.0	0.0	0.0	1.2336015799969036E-4
4	0.0	0.0	0.0	0.0	1.2336015799969036E-4
5	0.0	0.0	0.0	0.0	1.2336015799969036E-4
6	0.0	0.0	0.0	0.0	1.8504023699953556E-4
7	0.0	0.0	0.0	0.0	1.8504023699953556E-4
8	0.0	0.0	0.0	0.0	3.084003949992259E-4
9	0.0	0.0	0.0	0.0	3.084003949992259E-4
10-11	0.0	0.0	0.0	0.0	3.084003949992259E-4
12-13	0.0	0.0	0.0	0.0	4.6260059249883886E-4
14-15	0.0	0.0	0.0	0.0	5.24280671498684E-4
16-17	0.0	0.0	0.0	0.0	5.551207109986067E-4
18-19	0.0	0.0	0.0	6.168007899984518E-5	5.551207109986067E-4
20-21	0.0	0.0	0.0	1.5420019749961297E-4	7.401609479981422E-4
22-23	0.0	0.0	0.0	4.009205134989937E-4	8.018410269979874E-4
24-25	0.0	0.0	0.0	6.168007899984518E-4	8.3268106649791E-4
26-27	0.0	0.0	0.0	8.018410269979874E-4	8.943611454977551E-4
28-29	0.0	0.0	0.0	0.0028372836339928785	9.252011849976777E-4
30-31	0.0	0.0	0.0	0.010454773390473758	9.252011849976777E-4
32-33	0.0	0.0	0.0	0.027663515431430565	0.0010177213034974456
34-35	0.0	0.0	0.0	0.05005338410837437	0.0011719215009970585
36-37	0.0	0.0	0.0	0.07768605950030501	0.0011719215009970585
38-39	0.0	0.0	0.0	0.12539560060668525	0.0012336015799969036
40-41	0.0	0.0	0.0	0.18340571490603966	0.0012336015799969036
42-43	0.0	0.0	0.0	0.23885610592690049	0.0012336015799969036
44-45	0.0	0.0	0.0	0.2976989012927528	0.0012336015799969036
46-47	0.0	0.0	0.0	0.36378910594108693	0.0012336015799969036
48-49	0.0	0.0	0.0	0.43169887291991643	0.0012952816589967488
50-51	0.0	0.0	0.0	0.5029085241252377	0.0013261216984966715
52-53	0.0	0.0	0.0	0.5816431449685401	0.0014803218959962843
54-55	0.0	0.0	0.0	0.6719427806243135	0.0016036820539959747
56-57	0.0	0.0	0.0	0.7685646243775709	0.0016036820539959747
58-59	0.0	0.0	0.0	0.871354476030813	0.0016036820539959747
60-61	0.0	0.0	0.0	0.9744527280790541	0.0016036820539959747
62-63	0.0	0.0	0.0	1.0961166839062488	0.0016036820539959747
64-65	0.0	0.0	0.0	1.2120752324259576	0.0016962021724957425
66-67	0.0	0.0	0.0	1.3467228448826196	0.0017270422119956652
68-69	0.0	0.0	0.0	1.496790477089243	0.0017270422119956652
70-71	0.0	0.0	0.0	1.6516691554578542	0.0017887222909955102
72-73	0.0	0.0	0.0	1.794489378381996	0.001819562330495433
74-75	0.0	0.0	0.0	1.9423056877051248	0.0018504023699953554
76-77	0.0	0.0	0.0	2.1287645665216566	0.0018504023699953554
78-79	0.0	0.0	0.0	2.3374899538571325	0.0018504023699953554
80-81	0.0	0.0	0.0	2.528975759112152	0.001881242409495278
82-83	0.0	0.0	0.0	2.7314097783896445	0.0020046025674949684
84-85	0.0	0.0	0.0	2.9496338978910965	0.002035442606994891
86-87	0.0	0.0	0.0	3.20320070265946	0.0020971226859947366
88-89	0.0	0.0	0.0	3.4922643928922343	0.0022204828439944266
90-91	0.0	0.0	0.0	3.7846896474305005	0.0022821629229942716
92-93	0.0	0.0	0.0	4.066536768420293	0.002343843001994117
94-95	0.0	0.0	0.0	4.353379975809073	0.002343843001994117
96-97	0.0	0.0	0.0	4.678403152098758	0.0024672031599938075
98-99	0.0	0.0	0.0	5.052153590798319	0.0025288832389936526
100-101	0.0	0.0	0.0	5.435032681189858	0.0025288832389936526
102-103	0.0	0.0	0.0	5.823000378098884	0.0026214033574934203
104-105	0.0	0.0	0.0	6.228145977009367	0.002713923475993188
106-107	0.0	0.0	0.0	6.676498471259242	0.002713923475993188
108-109	0.0	0.0	0.0	7.1639561355950185	0.002713923475993188
110-111	0.0	0.0	0.0	7.691228290925196	0.002898963712992724
112-113	0.0	0.0	0.0	8.201075823937916	0.002960643791992569
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2510	0.0	56.55876	1
GTATCAA	4290	0.0	50.193398	1
TATCACG	190	0.0	40.808075	2
TCAACGC	5460	0.0	39.207047	4
TATCAAC	5655	0.0	37.968746	2
CAACGCA	5625	0.0	37.91463	5
GTACATG	8650	0.0	37.37488	1
ATCAACG	5770	0.0	37.10749	3
AACGCAG	5855	0.0	36.44438	6
TACATGG	8920	0.0	35.77217	2
ACATGGG	9105	0.0	34.65123	3
ATGGGGG	4900	0.0	33.555164	5
CATGGGG	8085	0.0	33.410313	4
ACGCAGA	7045	0.0	30.201004	7
CGCAGAG	7415	0.0	28.852976	8
TGGGGGG	3665	0.0	27.647087	6
GCAGAGT	7950	0.0	26.686413	9
GTGGTAT	1345	0.0	23.504457	1
AGAGTAC	7560	0.0	23.096872	10-11
GAGTACT	3745	0.0	22.49761	12-13
>>END_MODULE