FastQCFastQC Report
Mon 15 Aug 2016
SRR2921636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649304
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.043.1808471
GTATCAA37200.040.166521
GTACATG95300.035.2305641
TACATGG100300.033.1101572
ACATGGG98600.033.0170563
CATGGGG78900.032.8125654
TCAACGC44650.032.7900124
GTATACG1102.0333615E-532.4707261
CAACGCA46850.031.1222695
ATCAACG48250.030.5901993
ATGGGGG50650.030.5498185
AACGCAG49250.029.966356
TATCAAC49500.029.9379542
ACGCAGA57300.025.3309977
GTATAGG4550.024.8585381
CGCAGAG63450.022.779928
GGTCTCG7450.021.5568358
CCTATAC4201.8189894E-1221.255433
GCAGAGT67700.021.0869279
GAGTACA68300.020.5695461
Produced by FastQC (version 0.11.5)