##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921636_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1649304
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.029180187521526	31.0	31.0	34.0	26.0	34.0
2	30.088835047996003	31.0	31.0	34.0	26.0	34.0
3	30.23511311438037	31.0	31.0	34.0	26.0	34.0
4	33.643052463342116	37.0	35.0	37.0	28.0	37.0
5	33.70641616099882	37.0	35.0	37.0	29.0	37.0
6	33.55774071972177	37.0	35.0	37.0	28.0	37.0
7	33.51694411703361	37.0	35.0	37.0	28.0	37.0
8	33.52261863185926	37.0	35.0	37.0	28.0	37.0
9	35.14839653575084	39.0	37.0	39.0	28.0	39.0
10-11	35.17419317481799	39.0	37.0	39.0	28.0	39.0
12-13	35.1066401342627	39.0	37.0	39.0	27.0	39.0
14-15	36.386996272367014	40.0	38.0	41.0	27.0	41.0
16-17	36.34393689701838	40.0	38.0	41.0	27.0	41.0
18-19	36.294715225331416	40.0	38.0	41.0	27.0	41.0
20-21	36.33861374252412	40.0	38.0	41.0	27.0	41.0
22-23	36.260430763522066	40.0	38.0	41.0	27.0	41.0
24-25	36.23636303555924	40.0	38.0	41.0	27.0	41.0
26-27	36.18859925156308	40.0	37.5	41.0	27.0	41.0
28-29	36.1236470050397	40.0	37.0	41.0	27.0	41.0
30-31	36.02124653793358	40.0	37.0	41.0	27.0	41.0
32-33	35.92162360607868	40.0	37.0	41.0	26.0	41.0
34-35	35.7877577450852	40.0	37.0	41.0	25.0	41.0
36-37	35.67061621144434	40.0	36.0	41.0	25.0	41.0
38-39	35.55151233490005	40.0	36.0	41.0	24.0	41.0
40-41	35.437990813094494	40.0	36.0	41.0	24.0	41.0
42-43	35.169814358056485	40.0	35.0	41.0	23.0	41.0
44-45	34.79133622424975	39.5	35.0	41.0	21.5	41.0
46-47	34.52432571557457	39.0	35.0	41.0	19.5	41.0
48-49	34.31093297536415	39.0	34.5	41.0	18.5	41.0
50-51	34.0980064924356	39.0	34.0	40.5	17.0	41.0
52-53	33.89019034695848	38.0	34.0	40.0	15.0	41.0
54-55	33.64702413866698	38.0	34.0	40.0	13.0	41.0
56-57	33.39206750241314	38.0	33.0	40.0	10.5	41.0
58-59	33.39130020905788	38.0	34.0	40.0	9.0	41.0
60-61	33.17434566338285	37.0	33.0	40.0	8.0	41.0
62-63	32.859630789714934	36.5	33.0	40.0	7.0	41.0
64-65	32.56308964266139	36.0	33.0	39.0	4.0	41.0
66-67	32.24119780222445	35.5	33.0	39.0	2.0	41.0
68-69	31.910954257068436	35.0	32.5	38.5	2.0	40.0
70-71	31.55679668514719	35.0	32.0	37.5	2.0	40.0
72-73	31.212051265260982	35.0	32.0	37.0	2.0	39.0
74-75	30.847869161779755	35.0	31.5	36.5	2.0	39.0
76-77	30.56644317845588	35.0	31.0	36.0	2.0	38.5
78-79	30.29691706319757	35.0	31.0	35.5	2.0	37.0
80-81	30.041008813414628	35.0	31.0	35.0	2.0	37.0
82-83	29.816740576631112	35.0	31.0	35.0	2.0	36.5
84-85	29.609241231452785	35.0	31.0	35.0	2.0	36.0
86-87	29.443948780819063	34.5	31.0	35.0	2.0	36.0
88-89	29.278023032745935	34.0	30.0	35.0	2.0	35.5
90-91	29.08789737974321	34.0	30.0	35.0	2.0	35.0
92-93	29.00406929225904	34.0	30.0	35.0	2.0	35.0
94-95	28.867687521524232	34.0	30.0	35.0	2.0	35.0
96-97	28.778475647909666	34.0	30.0	35.0	2.0	35.0
98-99	28.6764692864384	34.0	29.0	35.0	2.0	35.0
100-101	28.561595982305263	34.0	29.0	35.0	2.0	35.0
102-103	27.66603761344179	33.0	28.0	34.5	2.0	35.0
104-105	28.59191210352973	34.0	29.5	35.0	2.0	35.0
106-107	28.868173484087833	34.0	30.0	35.0	2.0	35.0
108-109	28.857811234314596	35.0	31.0	35.0	2.0	35.0
110-111	28.786059756115307	35.0	30.0	35.0	2.0	35.0
112-113	28.692050101133567	35.0	30.0	35.0	2.0	35.0
114-115	28.561890652056867	34.0	30.0	35.0	2.0	35.0
116-117	28.42335160770846	34.0	30.0	35.0	2.0	35.0
118-119	28.128675186624175	34.0	29.5	35.0	2.0	35.0
120-121	27.979788140937025	34.0	29.0	35.0	2.0	35.0
122-123	27.7058850278663	34.0	29.0	35.0	2.0	35.0
124-125	26.910847545388844	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77836.0
3	13221.0
4	6472.0
5	5730.0
6	6066.0
7	6491.0
8	6395.0
9	6601.0
10	6901.0
11	7349.0
12	7205.0
13	7239.0
14	7475.0
15	6689.0
16	5753.0
17	5366.0
18	5093.0
19	4651.0
20	4753.0
21	4973.0
22	5813.0
23	7227.0
24	10237.0
25	12640.0
26	14448.0
27	16696.0
28	20063.0
29	23572.0
30	28666.0
31	36185.0
32	46980.0
33	65223.0
34	101165.0
35	164764.0
36	316934.0
37	463931.0
38	112475.0
39	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00308698505269	17.521035771424827	11.961672866917587	25.514204376604898
2	16.61922722421655	19.40635005336179	37.91385708741632	26.060565635005332
3	19.703217168581187	23.5516192564421	28.74404542455759	28.001118150419124
4	13.709328259105776	15.036219360894732	34.17893686200745	37.075515517992045
5	14.71856904013841	37.37949653703202	32.950014523786564	14.951919899043006
6	34.223572025092736	33.53143389850259	16.543157276311447	15.701836800093224
7	29.96754726093179	31.574349170601668	20.06324310189699	18.394860466569554
8	28.61167512228443	34.01621318955042	18.82194428452613	18.55016740363902
9	28.34934497816594	15.720099466278507	18.15950994662785	37.77104560892771
10-11	26.755925478800524	25.19698008359672	26.168139821280157	21.878954616322595
12-13	26.49952381630983	22.751695902212703	26.422418746325533	24.32636153515194
14-15	24.40107158149854	24.036710824622844	23.65224426817922	27.909973325699394
16-17	24.943029801615726	26.980491535899564	24.190877610969817	23.885601051514886
18-19	24.8564408492017	26.345974141306545	25.548357588685477	23.249227420806275
20-21	25.385040422193416	25.22700902456022	25.555202420297405	23.832748132948957
22-23	25.750180152419865	24.759796773512623	25.095626360241756	24.39439671382576
24-25	25.15072536201381	25.2242987479787	25.132104562763015	24.492871327244472
26-27	24.56059095012084	25.516329140339128	25.47083224140776	24.452247668132273
28-29	24.778240330004476	25.604191784769142	25.363209657483864	24.254358227742518
30-31	25.259775270813407	25.29728674107503	25.228419684515497	24.214518303596066
32-33	24.80456536756995	25.44807889075494	25.21745116484439	24.529904576830724
34-35	24.839580269920074	25.76285056366804	25.342950631123795	24.054618535288093
36-37	25.042507880027117	25.53741239192629	25.273815024625463	24.146264703421128
38-39	24.990122259590354	25.75365718979726	25.157475288971288	24.098745261641103
40-41	24.888042736813308	25.57129672064172	25.241544098802038	24.299116443742935
42-43	24.891797404835845	25.362421457788326	25.13183993081341	24.613941206562416
44-45	24.63517194705142	25.395513391178632	25.43675060461032	24.53256405715963
46-47	24.5969665331283	25.344007103177717	25.494931435109358	24.56409492858462
48-49	24.661361589339705	25.70494955223247	25.266279905503307	24.367408952924517
50-51	24.578760977142313	25.679522940476122	25.33757043900078	24.40414564338078
52-53	24.793229537199277	25.458709893019748	25.247345204932774	24.5007153648482
54-55	24.414085005888868	25.34823764581163	25.43654032994638	24.80113701835313
56-57	24.487569823084527	25.434222257264327	25.1305486987585	24.947659220892643
58-59	24.552635097908592	25.52212407627467	25.284974815967505	24.640266009849228
60-61	24.841376215226184	25.50680162778145	25.362520999229776	24.289301157762587
62-63	24.593072567828315	25.472685364743498	25.419436146576054	24.514805920852133
64-65	24.406210920964373	25.191491064963227	25.449329311262893	24.952968702809507
66-67	24.565338431324506	25.398907296055086	25.19981755919925	24.835936713421162
68-69	24.411995521814177	25.456460801925186	25.322703504556422	24.808840171704222
70-71	24.274107739736746	25.27735077836567	25.59931911961147	24.84922236228612
72-73	24.496704841561602	25.603682790245497	25.239946302244853	24.65966606594805
74-75	24.335038487239846	25.5383786969762	25.36816649869809	24.75841631708586
76-77	24.27367101588271	25.17106636802003	25.478859996197684	25.07640261989958
78-79	24.253774001729123	25.196057649940396	25.567082025953813	24.983086322376664
80-81	24.415133438390438	25.511895639907834	25.302185731539172	24.770785190162556
82-83	24.483665426598435	25.395521926403745	25.46099243465382	24.659820212344
84-85	24.33715805026456	25.460286485376926	25.2359458101597	24.966609654198816
86-87	24.147085168072298	25.59842155342479	25.401418952396142	24.85307432610677
88-89	24.17580918072158	25.262509930923226	25.435474773938832	25.126206114416366
90-91	24.400693680670972	25.099195790126117	25.533064606365937	24.96704592283697
92-93	24.51704054500638	25.182017457425165	25.463403985677513	24.83753801189094
94-95	24.38466795507041	25.095705516264026	25.580039404051618	24.93958712461395
96-97	24.26426756045926	24.996194670355358	25.56308266267557	25.17645510650981
98-99	23.951148863354888	25.13556032764291	25.463542798862584	25.449748010139622
100-101	24.001706370814873	25.31535115219445	25.617483084856474	25.065459392134198
102-103	23.903428989978366	25.151767610621185	25.74762071073081	25.197182688669635
104-105	23.868228417416137	25.19249280340525	25.64646567235674	25.29281310682187
106-107	23.871283616840024	25.153020854491494	25.687828361987165	25.287867166681323
108-109	23.881584931747753	25.140142993153553	25.673001704032067	25.30527037106663
110-111	23.693954003607026	25.595115206227682	25.650017901734785	25.060912888430508
112-113	23.55739934431239	25.608136007581578	25.75820321950255	25.07626142860348
114-115	23.584111890249172	25.81864831422075	25.566744863464873	25.030494932065206
116-117	23.416033502497136	25.627027320373237	25.67320148885165	25.283737688277974
118-119	23.40022240962405	25.375156638270628	25.823259133964406	25.401361818140916
120-121	23.336455908032598	25.658984526581296	25.840639551804422	25.163920013581688
122-123	23.301628704784978	25.616872998376266	25.782308223073503	25.299190073765253
124-125	23.195499743322117	25.54154775546748	25.811789421951676	25.451163079258727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	845.0
1	695.0
2	469.0
3	331.5
4	249.5
5	231.0
6	230.0
7	271.5
8	351.0
9	374.0
10	319.0
11	310.5
12	373.0
13	406.0
14	427.5
15	455.5
16	507.0
17	582.5
18	634.5
19	686.0
20	753.5
21	837.0
22	904.0
23	1007.5
24	1150.0
25	1354.0
26	1817.5
27	2482.0
28	3149.0
29	4078.0
30	5692.5
31	7729.0
32	10652.5
33	13747.5
34	16942.5
35	20273.5
36	24143.5
37	28275.5
38	31165.0
39	34302.0
40	37502.5
41	40324.0
42	43144.0
43	47277.5
44	52044.0
45	56047.5
46	59772.5
47	63560.0
48	68082.5
49	72132.5
50	73806.5
51	73999.0
52	72852.5
53	72030.0
54	72093.0
55	69687.5
56	65099.5
57	59754.0
58	54920.0
59	49342.0
60	42407.0
61	36731.5
62	31492.0
63	24809.0
64	18862.0
65	14823.5
66	11650.5
67	9324.0
68	7521.0
69	6158.0
70	5121.0
71	3936.5
72	3010.5
73	2602.0
74	2168.5
75	1704.0
76	1313.0
77	889.0
78	607.0
79	438.5
80	307.0
81	227.5
82	164.0
83	124.0
84	102.5
85	84.5
86	73.0
87	62.0
88	49.0
89	49.5
90	47.5
91	37.5
92	37.5
93	25.0
94	18.0
95	21.5
96	18.5
97	15.0
98	9.0
99	5.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02704170971512832
2	0.011156221048393746
3	0.009216008692151357
4	0.011277484320658896
5	0.017280016297783793
6	0.10040598943554373
7	0.06433016593666177
8	0.05414405106638922
9	0.030558344610817655
10-11	0.05541731542517329
12-13	0.015491383031872839
14-15	0.0318922406057343
16-17	0.03850108894418494
18-19	0.046534780731751095
20-21	0.035226980593025904
22-23	0.0424421452928023
24-25	0.03698529804087057
26-27	0.05093057435136276
28-29	0.05102152180556162
30-31	0.028800027162972988
32-33	0.032922978419988064
34-35	0.04947541508418097
36-37	0.012005063954249794
38-39	0.023919180454300724
40-41	0.048232466543463186
42-43	0.026041287718940837
44-45	0.018674543928833012
46-47	0.028163394983580954
48-49	0.03449940095943501
50-51	0.03255918860319262
52-53	0.03001265988562448
54-55	0.026071603537007125
56-57	0.029952028249491905
58-59	0.020766335375406838
60-61	0.026314130081537424
62-63	0.027496446986122632
64-65	0.024373917725295034
66-67	0.03334739987291609
68-69	0.02558655044794653
70-71	0.0515368907126885
72-73	0.027011393897062034
74-75	0.011277484320658896
76-77	0.018765491383031872
78-79	0.029739817523027894
80-81	0.02179707318966061
82-83	0.028496868982310113
84-85	0.01512759321507739
86-87	0.020675387921207978
88-89	0.025101497358885932
90-91	0.02537433972148252
92-93	0.009034113783753631
94-95	0.014248434491155057
96-97	0.018250122475904986
98-99	0.007912428515300999
100-101	0.011520010865189195
102-103	0.004365477801545379
104-105	0.005244636525467712
106-107	0.0011520010865189196
108-109	0.01661306830032547
110-111	0.001364211812982931
112-113	0.0026071603537007127
114-115	0.015733909576403138
116-117	0.00688169070104723
118-119	0.2783598414846505
120-121	0.001455159267181793
122-123	0.0010913694503863448
124-125	0.31649714061204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1649304.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.619750386609496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39909006080754	50.15791611244107
2	10.008155286717438	12.334000611959093
3	3.3512895353635628	6.195168739270779
4	1.5781598369457213	3.8898326089107074
5	0.895972087657422	2.7604788197409875
6	0.5639232572373539	2.084928620890172
7	0.40531136560447034	1.7482629624182302
8	0.2961402214825818	1.4598469221753552
9	0.2206889072936292	1.2238915842474334
>10	1.198519622970349	13.290591608244961
>50	0.06242237294484713	2.62628202956108
>100	0.0197383237078787	1.9573851245140426
>500	3.9274751148178817E-4	0.14078933857371828
>1k	1.9637375574089408E-4	0.13062491705228094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.063163613257471E-5
2	0.0	0.0	0.0	0.0	6.063163613257471E-5
3	0.0	0.0	0.0	0.0	6.063163613257471E-5
4	0.0	0.0	0.0	0.0	6.063163613257471E-5
5	0.0	0.0	0.0	0.0	6.063163613257471E-5
6	0.0	0.0	0.0	0.0	1.8189490839772414E-4
7	0.0	0.0	0.0	0.0	1.8189490839772414E-4
8	0.0	0.0	0.0	0.0	1.8189490839772414E-4
9	0.0	0.0	0.0	0.0	1.8189490839772414E-4
10-11	0.0	0.0	0.0	0.0	1.8189490839772414E-4
12-13	0.0	0.0	0.0	0.0	3.0315818066287354E-4
14-15	0.0	0.0	0.0	0.0	3.637898167954483E-4
16-17	0.0	0.0	0.0	0.0	4.547372709943103E-4
18-19	0.0	0.0	0.0	0.0	5.456847251931724E-4
20-21	0.0	0.0	0.0	6.063163613257471E-5	6.669479974583219E-4
22-23	0.0	0.0	0.0	3.3347399872916093E-4	7.578954516571838E-4
24-25	0.0	0.0	0.0	6.669479974583219E-4	8.791587239223332E-4
26-27	0.0	0.0	0.0	0.0014248434491155057	9.701061781211954E-4
28-29	0.0	0.0	0.0	0.005426531433865437	9.701061781211954E-4
30-31	0.0	0.0	0.0	0.022009283916124618	0.0010004219961874828
32-33	0.0	0.0	0.0	0.05171878562108623	0.0010913694503863448
34-35	0.0	0.0	0.0	0.08934071584134884	0.0011520010865189196
36-37	0.0	0.0	0.0	0.14312097709094262	0.0011520010865189196
38-39	0.0	0.0	0.0	0.22433705369052642	0.0011520010865189196
40-41	0.0	0.0	0.0	0.31892240605734296	0.0011520010865189196
42-43	0.0	0.0	0.0	0.40462522373073734	0.0011520010865189196
44-45	0.0	0.0	0.0	0.5071230046128549	0.0011823169045852067
46-47	0.0	0.0	0.0	0.6206254274530347	0.0012126327226514941
48-49	0.0	0.0	0.0	0.7331577441150934	0.0012126327226514941
50-51	0.0	0.0	0.0	0.8417490044285347	0.0012126327226514941
52-53	0.0	0.0	0.0	0.9601929056135194	0.0013642118129829311
54-55	0.0	0.0	0.0	1.0909147131153505	0.001455159267181793
56-57	0.0	0.0	0.0	1.2341569534785581	0.001455159267181793
58-59	0.0	0.0	0.0	1.3834017258188909	0.001455159267181793
60-61	0.0	0.0	0.0	1.5315551287088371	0.001455159267181793
62-63	0.0	0.0	0.0	1.6858626426662398	0.001455159267181793
64-65	0.0	0.0	0.0	1.8504472189481138	0.001455159267181793
66-67	0.0	0.0	0.0	2.034888656063406	0.0014854750852480803
68-69	0.0	0.0	0.0	2.2362463196596867	0.0015157909033143677
70-71	0.0	0.0	0.0	2.4439703050498878	0.0016370541755795173
72-73	0.0	0.0	0.0	2.6523006068014143	0.001879580720109816
74-75	0.0	0.0	0.0	2.87166586632907	0.0019402123562423908
76-77	0.0	0.0	0.0	3.1230446297347245	0.0019402123562423908
78-79	0.0	0.0	0.0	3.3964023612384375	0.0019402123562423908
80-81	0.0	0.0	0.0	3.669184092198891	0.0020614756285075404
82-83	0.0	0.0	0.0	3.9535161498426	0.0022433705369052643
84-85	0.0	0.0	0.0	4.247488637631388	0.0023646338091704135
86-87	0.0	0.0	0.0	4.589329802146845	0.0025162128995018505
88-89	0.0	0.0	0.0	4.9578185707425675	0.0026677919898332875
90-91	0.0	0.0	0.0	5.336735980753094	0.0026677919898332875
92-93	0.0	0.0	0.0	5.722353186556269	0.002728423625965862
94-95	0.0	0.0	0.0	6.120945562491815	0.0027890552620984366
96-97	0.0	0.0	0.0	6.559827660637457	0.0030012659885624484
98-99	0.0	0.0	0.0	7.042546431706951	0.00309221344276131
100-101	0.0	0.0	0.0	7.5634328177219	0.00309221344276131
102-103	0.0	0.0	0.0	8.095263214058779	0.0031831608969601724
104-105	0.0	0.0	0.0	8.642797204153995	0.0032741083511590345
106-107	0.0	0.0	0.0	9.222253750673012	0.0032741083511590345
108-109	0.0	0.0	0.0	9.840635807589141	0.0033044241692253215
110-111	0.0	0.0	0.0	10.495912215091941	0.003334739987291609
112-113	0.0	0.0	0.0	11.147095987155794	0.003334739987291609
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2385	0.0	43.180847	1
GTATCAA	3720	0.0	40.16652	1
GTACATG	9530	0.0	35.230564	1
TACATGG	10030	0.0	33.110157	2
ACATGGG	9860	0.0	33.017056	3
CATGGGG	7890	0.0	32.812565	4
TCAACGC	4465	0.0	32.790012	4
GTATACG	110	2.0333615E-5	32.470726	1
CAACGCA	4685	0.0	31.122269	5
ATCAACG	4825	0.0	30.590199	3
ATGGGGG	5065	0.0	30.549818	5
AACGCAG	4925	0.0	29.96635	6
TATCAAC	4950	0.0	29.937954	2
ACGCAGA	5730	0.0	25.330997	7
GTATAGG	455	0.0	24.858538	1
CGCAGAG	6345	0.0	22.77992	8
GGTCTCG	745	0.0	21.556835	8
CCTATAC	420	1.8189894E-12	21.25543	3
GCAGAGT	6770	0.0	21.086927	9
GAGTACA	6830	0.0	20.569546	1
>>END_MODULE