FastQCFastQC Report
Mon 15 Aug 2016
SRR2921636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649304
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.16431173391927748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.12890285841785384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.046.1785051
GTATCAA47700.044.1414531
TCAACGC57900.035.8403434
CAACGCA59600.034.884185
GTACATG95150.034.0393561
ATCAACG61400.033.8975373
AACGCAG62550.033.630136
TATCAAC62450.033.615112
TACATGG99300.032.5517462
ACATGGG99250.031.9662863
CATGGGG79900.030.972254
ATGGGGG47350.030.950275
ACGCAGA75550.027.919687
TACCGTA1306.317733E-527.5010647
CGCAGAG80950.025.9804538
GTATCGC1159.960692E-425.909166
GCAGAGT88950.023.443599
ACCGTAT1852.6641594E-522.543188
GAGTACT34900.022.08872612-13
TGGGGGG36200.021.8939576
Produced by FastQC (version 0.11.5)