##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1649304
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679415680796264	33.0	31.0	34.0	30.0	34.0
2	31.84580344193672	33.0	31.0	34.0	30.0	34.0
3	31.91607429558165	34.0	31.0	34.0	30.0	34.0
4	35.30165572871951	37.0	35.0	37.0	33.0	37.0
5	35.15851474318864	37.0	35.0	37.0	33.0	37.0
6	35.18757730533607	37.0	35.0	37.0	33.0	37.0
7	35.07681179455091	37.0	35.0	37.0	33.0	37.0
8	35.110871009832024	37.0	35.0	37.0	33.0	37.0
9	36.81441747549269	39.0	38.0	39.0	33.0	39.0
10-11	36.772412787454584	39.0	38.0	39.0	33.0	39.0
12-13	36.70289710083769	39.0	38.0	39.0	33.0	39.0
14-15	38.12319954356504	41.0	39.0	41.0	33.0	41.0
16-17	38.1286670013533	41.0	39.0	41.0	33.0	41.0
18-19	38.14611951465588	41.0	39.0	41.0	33.5	41.0
20-21	38.08227500812464	41.0	39.0	41.0	33.0	41.0
22-23	38.082306839733604	41.0	39.0	41.0	34.0	41.0
24-25	38.04404403311942	41.0	39.0	41.0	33.0	41.0
26-27	37.96894023175837	41.0	39.0	41.0	33.0	41.0
28-29	37.89712812192295	41.0	38.5	41.0	33.0	41.0
30-31	37.77874879343044	41.0	38.0	41.0	33.0	41.0
32-33	37.654085905327335	41.0	38.0	41.0	33.0	41.0
34-35	37.517103275078455	41.0	38.0	41.0	32.0	41.0
36-37	37.43492558072981	40.5	38.0	41.0	32.0	41.0
38-39	37.346882988218056	40.0	38.0	41.0	32.0	41.0
40-41	37.2264931146714	40.0	38.0	41.0	32.0	41.0
42-43	37.06059101293637	40.0	37.0	41.0	31.0	41.0
44-45	36.905666268923135	40.0	37.0	41.0	31.0	41.0
46-47	36.69789862875491	40.0	37.0	41.0	30.5	41.0
48-49	36.503709140340405	40.0	36.0	41.0	30.0	41.0
50-51	36.259719554430234	40.0	36.0	41.0	30.0	41.0
52-53	36.12480415981529	40.0	35.0	41.0	29.0	41.0
54-55	35.99051690895068	39.5	35.0	41.0	29.0	41.0
56-57	35.78914954429262	39.0	35.0	41.0	29.0	41.0
58-59	35.5959322841635	39.0	35.0	41.0	29.0	41.0
60-61	35.340354476797486	38.5	35.0	41.0	28.0	41.0
62-63	34.98154888365032	37.5	35.0	40.5	28.0	41.0
64-65	34.69255728476982	37.0	35.0	40.0	28.0	41.0
66-67	34.39403075479111	36.5	35.0	39.5	28.0	41.0
68-69	34.02223180202073	36.0	35.0	39.0	27.5	41.0
70-71	33.65371211129058	35.5	34.5	39.0	26.0	41.0
72-73	33.34120726075969	35.0	34.0	37.5	26.0	40.0
74-75	33.02191924593647	35.0	34.0	37.0	26.0	39.0
76-77	32.70878655481342	35.0	34.0	36.5	26.0	39.0
78-79	32.456703251795915	35.0	34.0	36.0	26.0	38.0
80-81	32.211941825157766	35.0	34.0	36.0	26.0	37.0
82-83	31.992180944204343	35.0	34.0	35.0	25.0	37.0
84-85	31.784441194588748	35.0	34.0	35.0	24.5	36.0
86-87	31.66623860731557	35.0	34.0	35.0	25.0	36.0
88-89	31.570620698185415	35.0	33.5	35.0	24.5	36.0
90-91	31.470534237472293	35.0	33.0	35.0	24.0	35.5
92-93	31.39687892589844	35.0	33.0	35.0	24.0	35.0
94-95	31.329997987029678	35.0	33.0	35.0	24.0	35.0
96-97	31.264873546659683	35.0	33.0	35.0	23.5	35.0
98-99	31.190631927164425	35.0	33.0	35.0	23.0	35.0
100-101	31.12319348040143	35.0	33.0	35.0	22.0	35.0
102-103	30.431693005049404	34.0	31.5	35.0	20.0	35.0
104-105	30.94621367558679	35.0	33.0	35.0	20.0	35.0
106-107	31.03504538884281	35.0	33.0	35.0	20.0	35.0
108-109	31.020166385335877	35.0	33.0	35.0	20.0	35.0
110-111	30.949328019576743	35.0	33.0	35.0	19.0	35.0
112-113	30.90326374034138	35.0	33.0	35.0	19.0	35.0
114-115	30.771566369814177	35.0	33.0	35.0	18.0	35.0
116-117	30.94645408002406	35.0	33.0	35.0	19.0	35.0
118-119	30.922875043048464	35.0	33.0	35.0	17.5	35.0
120-121	30.833366680733207	35.0	33.0	35.0	11.0	35.0
122-123	30.685151433574404	35.0	33.0	35.0	2.0	35.0
124-125	30.039215329617825	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29251.0
3	7510.0
4	4098.0
5	3234.0
6	3502.0
7	3936.0
8	4113.0
9	4338.0
10	4634.0
11	5063.0
12	5632.0
13	6914.0
14	9014.0
15	8235.0
16	4846.0
17	4347.0
18	3901.0
19	3579.0
20	3392.0
21	3547.0
22	3881.0
23	4710.0
24	6282.0
25	8002.0
26	9716.0
27	11645.0
28	13974.0
29	17032.0
30	20703.0
31	25749.0
32	34324.0
33	46596.0
34	72933.0
35	130436.0
36	297508.0
37	607414.0
38	215093.0
39	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.729854201272715	17.86797031020967	11.462917781792534	26.93925770672508
2	15.992488788965773	19.709228578981705	37.43660824074681	26.86167439130572
3	18.799457772683024	23.6188518002978	28.56512944615786	29.016560980861318
4	13.43845070413981	14.973728755397131	34.171810477765554	37.4160100626975
5	14.652559364322338	37.503320423607214	32.57732796566894	15.26679224640151
6	34.74058302453611	33.811794587700334	16.459766962509274	14.987855425254285
7	30.448577753537947	31.75667928337731	19.286823191532346	18.5079197715524
8	28.441110492364547	34.119020148086676	18.573983284136915	18.86588607541186
9	28.383955280913597	14.98769933984031	18.393247495590444	38.23509788365565
10-11	27.03639504216369	24.82242954364681	26.101992514344268	22.039182899845226
12-13	26.94283465259588	22.309364160446997	26.61523084693167	24.13257034002545
14-15	24.50771406964155	23.55525193469819	24.08696593881588	27.85006805684438
16-17	24.513833131266193	26.634582631002147	24.785311877513642	24.06627236021802
18-19	24.461774837393616	25.76644731055742	26.627534782268487	23.144243069780476
20-21	25.220062734709327	24.764229328597906	26.49116231453718	23.52454562215559
22-23	25.168343456729115	24.246763267156084	26.225653447891673	24.359239828223128
24-25	24.655642079381273	24.65433841107087	26.22841178334245	24.4616077262054
26-27	24.216885258376404	24.979422233931377	26.49033807564311	24.31335443204911
28-29	24.36165281298423	25.216551898935275	26.31353798401104	24.108257304069454
30-31	24.91450680125681	24.82422355804503	26.191023335398917	24.070246305299246
32-33	24.531896128041115	24.874471424720905	26.15251164783296	24.44112079940502
34-35	24.58271928024135	25.07771100208311	26.327514318136547	24.012055399538994
36-37	24.653984929990518	24.88052319667566	26.328160931911487	24.137330941422334
38-39	24.65053939473314	25.137546869618788	26.18614090232202	24.025772833326055
40-41	24.71289562497802	24.928178430366728	26.191558949470846	24.16736699518441
42-43	24.5904198925613	24.780479493481614	26.005452882408918	24.623647731548164
44-45	24.411167623496542	24.89622215916119	26.081436045200206	24.61117417214206
46-47	24.45049048082765	24.87863798040017	26.041081786419824	24.629789752352355
48-49	24.428243229232034	25.248520699295867	25.820413894967377	24.502822176504722
50-51	24.476086072071933	25.254976598723434	25.86079044716174	24.408146882042896
52-53	24.649991738831886	25.131261624373025	25.70984655448657	24.508900082308518
54-55	24.243713211162433	24.91864958153763	25.928355846876578	24.909281360423357
56-57	24.32566970707601	25.029429428336954	25.643301686276686	25.00159917831035
58-59	24.427540159802486	25.075365946233703	25.833845676250206	24.663248217713605
60-61	24.62900203510395	25.100625931644394	25.898236313978977	24.372135719272674
62-63	24.487702867966583	25.080565116926284	25.915168190860655	24.516563824246475
64-65	24.214577785538626	24.805766553649296	25.975987446826053	25.003668213986018
66-67	24.359715567251126	25.12370035425247	25.646302328789343	24.87028174970706
68-69	24.264783678587225	25.106871518016654	25.750116640852582	24.878228162543536
70-71	24.207938621382112	24.962226490689407	25.88749557389056	24.94233931403792
72-73	24.449685844430466	25.33020061997408	25.504793809354183	24.715319726241276
74-75	24.235162790246438	25.347080004620352	25.510520557098538	24.907236648034665
76-77	24.169865816106736	24.924686216569608	25.61438675920477	25.291061208118883
78-79	24.13095733615554	25.094062604116107	25.664637826763524	25.110342232964822
80-81	24.27842289838623	25.323105988950488	25.420419764943276	24.978051347720008
82-83	24.368051108912045	25.268526738071678	25.4850883831034	24.878333769912878
84-85	24.162535531000493	25.290675113262644	25.27318246199517	25.273606893741697
86-87	23.96535144521568	25.51191290386733	25.415720813143	25.107014837773995
88-89	23.959433244112663	25.12527424821051	25.50789540778818	25.40739709988865
90-91	24.07079431389966	24.929196591250147	25.65214623306463	25.347862861785565
92-93	24.181776070390903	25.042411829474737	25.58088138996813	25.19493071016623
94-95	24.08589326068542	24.998923742127403	25.606296987751275	25.3088860094359
96-97	23.91985545119962	24.891952197032627	25.62336670163163	25.564825650136118
98-99	23.669625311724857	24.953328430216377	25.50195811412298	25.875088143935788
100-101	23.68641560318777	25.190534916546614	25.595766456638678	25.527283023626936
102-103	23.698608334230187	24.983955144130856	25.66658531816108	25.650851203477878
104-105	23.74983629458244	25.097495670901182	25.491176884309986	25.661491150206388
106-107	23.84133987636472	24.961672820337764	25.468467728317666	25.728519574979845
108-109	23.73740711658971	24.99113230599456	25.489784719674763	25.781675857740964
110-111	23.549500470653502	25.427658055450713	25.399555266544493	25.62328620735129
112-113	23.428399060812254	25.46611511870963	25.443893536490535	25.661592283987577
114-115	23.383499949069424	25.59027929356868	25.314920718072592	25.7113000392893
116-117	23.310784898359238	25.44885917328603	25.35014850559143	25.8902074227633
118-119	23.29452282170256	25.16646177586513	25.461474213317853	26.077541189114463
120-121	23.166045523295985	25.388822208485283	25.45357917129884	25.99155309691989
122-123	23.18317732019583	25.525420738282644	25.20967565415924	26.081726287362283
124-125	22.995948222225596	25.46200889266583	25.211466379349588	26.33057650575899
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	85.0
2	73.5
3	104.5
4	94.0
5	88.0
6	189.0
7	499.0
8	851.5
9	925.0
10	728.0
11	551.5
12	508.0
13	519.5
14	584.5
15	657.5
16	691.5
17	688.0
18	742.5
19	829.5
20	901.0
21	946.5
22	984.0
23	1076.0
24	1205.5
25	1422.5
26	1851.5
27	2463.0
28	3136.5
29	4104.5
30	5755.5
31	7769.5
32	10723.5
33	13600.0
34	16521.5
35	20095.0
36	24014.5
37	27780.5
38	30673.0
39	34102.0
40	37345.0
41	40156.0
42	42995.5
43	46958.5
44	51815.5
45	55954.5
46	59847.0
47	63596.5
48	68192.5
49	72534.5
50	74273.0
51	74566.5
52	73837.5
53	73229.5
54	73202.5
55	71187.0
56	66334.0
57	60490.0
58	55472.5
59	49710.5
60	42732.5
61	36755.5
62	31179.5
63	24354.0
64	18252.5
65	14209.5
66	11056.0
67	8642.0
68	6930.0
69	5631.0
70	4559.0
71	3461.0
72	2679.0
73	2380.0
74	1995.0
75	1558.5
76	1084.0
77	627.5
78	386.5
79	227.0
80	127.0
81	75.0
82	47.0
83	21.5
84	12.5
85	8.0
86	5.0
87	5.0
88	4.5
89	3.0
90	2.5
91	1.0
92	1.0
93	1.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023464443183306413
2	0.0021827389007726896
3	0.03328676823678352
4	0.1714056353467887
5	0.025344023903416227
6	0.07536512371279036
7	0.058267002323404295
8	0.049111625267385516
9	0.03710656131313572
10-11	0.06578532520384356
12-13	0.05890363450279633
14-15	0.06363290212113716
16-17	0.05605394760456532
18-19	0.04189646056760912
20-21	0.027648026076454068
22-23	0.004335161983479092
24-25	0.00676042742878208
26-27	0.004395793619611666
28-29	0.006275374339721483
30-31	0.003031581806628736
32-33	0.010762115413532012
34-35	0.005123373253202563
36-37	0.008154955059831298
38-39	0.002910318534363586
40-41	0.004729267618340827
42-43	0.005123373253202563
44-45	0.006548216702318068
46-47	0.0064269534300529195
48-49	0.002728423625965862
50-51	0.0021221072646401148
52-53	0.0013035801768503563
54-55	0.00676042742878208
56-57	0.0011823169045852067
58-59	0.0010913694503863448
60-61	1.5157909033143677E-4
62-63	0.00103073781425377
64-65	0.0
66-67	0.003425687441490471
68-69	6.366321793920345E-4
70-71	0.0
72-73	0.0025162128995018505
74-75	0.004880846708672264
76-77	0.0022736863549715517
78-79	2.1221072646401148E-4
80-81	0.0
82-83	0.007275796335908965
84-85	0.0024252654453029883
86-87	0.0
88-89	0.001364211812982931
90-91	0.0011520010865189196
92-93	0.0
94-95	0.004304846165412804
96-97	0.00206147562850754
98-99	7.882112697234712E-4
100-101	0.0
102-103	0.0013035801768503563
104-105	0.0
106-107	0.0010610536323200574
108-109	0.0035772665318219076
110-111	9.094745419886207E-5
112-113	3.941056348617356E-4
114-115	0.0
116-117	0.002394949627236701
118-119	0.0031225292608275976
120-121	0.00366821398602077
122-123	0.0018492649020435286
124-125	0.11392684429310788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1649304.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.87662795859958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50270147574402	45.43471647056643
2	11.641168626885065	13.475031712430969
3	3.7967430588702515	6.592280564178867
4	1.821908621517843	4.217837098486128
5	1.0520166999313012	3.0443589574078804
6	0.6391662362679323	2.2195671876106484
7	0.4478699704706111	1.814484255832958
8	0.34510811222742777	1.5978955053505173
9	0.24388309077197287	1.270361781900256
>10	1.4098814259608654	14.563516497436021
>50	0.07442447990087196	2.9007761129912617
>100	0.02428717561784649	2.263830192937313
>500	4.2051291701816426E-4	0.14578383920377402
>1k	4.2051291701816426E-4	0.4595598236669889
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2710	0.16431173391927748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.12890285841785384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.063163613257471E-5
7	0.0	0.0	0.0	0.0	6.063163613257471E-5
8	0.0	0.0	0.0	0.0	6.063163613257471E-5
9	0.0	0.0	0.0	0.0	6.063163613257471E-5
10-11	0.0	0.0	0.0	0.0	6.063163613257471E-5
12-13	0.0	0.0	0.0	0.0	2.4252654453029885E-4
14-15	0.0	0.0	0.0	0.0	2.728423625965862E-4
16-17	0.0	0.0	0.0	0.0	3.0315818066287354E-4
18-19	0.0	0.0	0.0	0.0	3.0315818066287354E-4
20-21	0.0	0.0	0.0	9.094745419886207E-5	3.637898167954483E-4
22-23	0.0	0.0	0.0	3.941056348617356E-4	5.15368907126885E-4
24-25	0.0	0.0	0.0	7.275796335908966E-4	6.669479974583219E-4
26-27	0.0	0.0	0.0	0.0014248434491155057	7.275796335908966E-4
28-29	0.0	0.0	0.0	0.005426531433865437	7.578954516571838E-4
30-31	0.0	0.0	0.0	0.02188802064385947	7.882112697234712E-4
32-33	0.0	0.0	0.0	0.0517794172572188	7.882112697234712E-4
34-35	0.0	0.0	0.0	0.09000766383880715	7.882112697234712E-4
36-37	0.0	0.0	0.0	0.1450308736291187	7.882112697234712E-4
38-39	0.0	0.0	0.0	0.22758084622361918	7.882112697234712E-4
40-41	0.0	0.0	0.0	0.32498556967060044	7.882112697234712E-4
42-43	0.0	0.0	0.0	0.41174944097631483	7.882112697234712E-4
44-45	0.0	0.0	0.0	0.5140956427681009	7.882112697234712E-4
46-47	0.0	0.0	0.0	0.6287803825128659	8.488429058560459E-4
48-49	0.0	0.0	0.0	0.742404068625311	9.701061781211954E-4
50-51	0.0	0.0	0.0	0.8519957509349398	9.701061781211954E-4
52-53	0.0	0.0	0.0	0.9725011277484321	0.0010913694503863448
54-55	0.0	0.0	0.0	1.1049812526981078	0.0011520010865189196
56-57	0.0	0.0	0.0	1.2495877048742985	0.0011520010865189196
58-59	0.0	0.0	0.0	1.399863215028885	0.0011520010865189196
60-61	0.0	0.0	0.0	1.54998714609314	0.0011520010865189196
62-63	0.0	0.0	0.0	1.7056285560454592	0.0011520010865189196
64-65	0.0	0.0	0.0	1.8732143983158958	0.0011520010865189196
66-67	0.0	6.063163613257471E-5	0.0	2.061324049417209	0.0011520010865189196
68-69	0.0	6.063163613257471E-5	0.0	2.2684720342641502	0.0011520010865189196
70-71	0.0	6.063163613257471E-5	0.0	2.47971265455004	0.0011520010865189196
72-73	0.0	6.063163613257471E-5	0.0	2.6924993815573113	0.001273264358784069
74-75	0.0	6.063163613257471E-5	0.0	2.9168667510659043	0.0013035801768503563
76-77	0.0	6.063163613257471E-5	0.0	3.1741570989944847	0.0013945276310492183
78-79	0.0	6.063163613257471E-5	0.0	3.4535779941114555	0.0013945276310492183
80-81	0.0	6.063163613257471E-5	0.0	3.732574467775498	0.0014248434491155057
82-83	0.0	6.063163613257471E-5	0.0	4.023848847756387	0.0014854750852480803
84-85	0.0	6.063163613257471E-5	0.0	4.325976290605007	0.0015157909033143677
86-87	0.0	6.063163613257471E-5	0.0	4.674638514185377	0.001546106721380655
88-89	0.0	6.063163613257471E-5	0.0	5.054616977828223	0.0015764225394469425
90-91	0.0	6.063163613257471E-5	0.0	5.444751240523276	0.0015764225394469425
92-93	0.0	6.063163613257471E-5	0.0	5.842282562826501	0.0016673699936458047
94-95	0.0	6.063163613257471E-5	0.0	6.253092213442761	0.0016976858117120918
96-97	0.0	6.063163613257471E-5	0.0	6.702978953546466	0.0018189490839772412
98-99	0.0	6.063163613257471E-5	0.0	7.197975630932806	0.0019402123562423908
100-101	0.0	6.063163613257471E-5	0.0	7.729169395090293	0.0019402123562423908
102-103	0.0	6.063163613257471E-5	0.0	8.269003167396672	0.0020311598104412526
104-105	0.0	6.063163613257471E-5	0.0	8.826541377453761	0.00206147562850754
106-107	0.0	6.063163613257471E-5	0.0	9.41897309410515	0.00206147562850754
108-109	0.0	6.063163613257471E-5	0.0	10.052664639144755	0.0020917914465738274
110-111	0.0	6.063163613257471E-5	0.0	10.724432851675616	0.002152423082706402
112-113	0.0	6.063163613257471E-5	0.0	11.394321483486367	0.0021827389007726896
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2945	0.0	46.178505	1
GTATCAA	4770	0.0	44.141453	1
TCAACGC	5790	0.0	35.840343	4
CAACGCA	5960	0.0	34.88418	5
GTACATG	9515	0.0	34.039356	1
ATCAACG	6140	0.0	33.897537	3
AACGCAG	6255	0.0	33.63013	6
TATCAAC	6245	0.0	33.61511	2
TACATGG	9930	0.0	32.551746	2
ACATGGG	9925	0.0	31.966286	3
CATGGGG	7990	0.0	30.97225	4
ATGGGGG	4735	0.0	30.95027	5
ACGCAGA	7555	0.0	27.91968	7
TACCGTA	130	6.317733E-5	27.501064	7
CGCAGAG	8095	0.0	25.980453	8
GTATCGC	115	9.960692E-4	25.90916	6
GCAGAGT	8895	0.0	23.44359	9
ACCGTAT	185	2.6641594E-5	22.54318	8
GAGTACT	3490	0.0	22.088726	12-13
TGGGGGG	3620	0.0	21.893957	6
>>END_MODULE