FastQCFastQC Report
Mon 15 Aug 2016
SRR2921635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392001
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC801.1959059E-437.1964453
GGTATCA19850.033.587111
GTATCAA29900.033.247611
GTACATG68900.032.053081
GTCTCGT6050.031.4639369
TACATGG71900.030.5435052
TCAACGC32500.030.2149584
ACATGGG72450.029.5723273
GGTCTCG6650.029.5185598
CATGGGG56700.029.494754
CAACGCA33700.029.1380065
AACGCAG36250.026.9221926
TATCAAC36350.026.8510232
ATCAACG38100.025.7739143
ATGGGGG35600.025.5768595
GTATAGG4050.023.5169011
ACGCAGA43350.022.50477
ACCGTAT1703.9411912E-420.994488
CGCAGAG45650.020.9791538
AGTACAT49250.020.7846932
Produced by FastQC (version 0.11.5)