FastQCFastQC Report
Mon 15 Aug 2016
SRR2921635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392001
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.19015790936931798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.15007173127030798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.10538785532481659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43800.043.9735031
GGTATCA28800.043.687041
TCAACGC54550.034.640684
CAACGCA56250.033.670655
TATCAAC58200.032.5764852
AACGCAG58400.032.3430376
ATCAACG58950.032.060893
GTACATG73200.029.8963131
ACATGGG74800.028.3758243
TACATGG76950.028.2028392
CATGGGG57550.028.0702254
TAGCCCG1306.3363535E-527.4887375
ACGCAGA68600.027.3602967
CGCAGAG70300.026.8672168
CCTATAC3450.025.9222243
ATGGGGG30300.024.3739515
GCAGAGT79250.023.6826369
GAGTACT36250.022.41573112-13
AGTACTT38050.022.29402412-13
GTACTTT36900.022.17582114-15
Produced by FastQC (version 0.11.5)