FastQCFastQC Report
Mon 15 Aug 2016
SRR2921634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091289
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.041.081571
GTATCAA39650.040.383311
GTACATG101950.038.009011
TACATGG107350.035.8120962
CATGGGG93000.034.234984
ACATGGG111250.034.128713
TCAACGC46350.032.6124344
CAACGCA46400.032.5772935
ATGGGGG60450.031.6999685
ATCAACG49650.030.5647053
AACGCAG50050.030.3168016
TATCAAC51150.030.1337642
ACGCAGA59400.025.7376697
GTATAGG6900.025.0173761
TAGAGAG15750.024.182362
CGCAGAG64300.023.4976548
TGGGGGG48900.021.7816856
TATAGGG4700.021.5253542
GTATAGA7750.021.505481
GAGTACA73200.020.7358511
Produced by FastQC (version 0.11.5)