FastQCFastQC Report
Mon 15 Aug 2016
SRR2921634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091289
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.1601882857892907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.12657265447291122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.050.087861
GTATCAA51300.044.195171
GTACATG102350.035.8505551
TCAACGC65350.034.49924
TACATGG105000.034.4260942
CAACGCA66150.034.1320425
TATCAAC68550.032.8918882
ATCAACG69300.032.449843
ACATGGG110000.032.3732343
AACGCAG70350.032.1012236
CATGGGG91800.031.7708594
ATGGGGG54050.029.86945
GTATAGG5400.027.6219831
ACGCAGA83100.027.1746447
CGTATAC1358.1100254E-526.5107463
CGCAGAG87200.025.8261348
GCAGAGT98350.022.7770549
TGGGGGG38450.022.315816
GTACCGT4850.022.1145086
AGAGTAC88350.021.91598110-11
Produced by FastQC (version 0.11.5)