##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2091289
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70705961729823	33.0	31.0	34.0	30.0	34.0
2	31.877312031000976	33.0	31.0	34.0	30.0	34.0
3	31.94897214110532	34.0	31.0	34.0	30.0	34.0
4	35.34376884304369	37.0	35.0	37.0	33.0	37.0
5	35.200534215978756	37.0	35.0	37.0	33.0	37.0
6	35.23605106706916	37.0	35.0	37.0	33.0	37.0
7	35.12072171756271	37.0	35.0	37.0	33.0	37.0
8	35.15162371150042	37.0	36.0	37.0	33.0	37.0
9	36.85595725889631	39.0	38.0	39.0	34.0	39.0
10-11	36.80549388439378	39.0	38.0	39.0	33.0	39.0
12-13	36.737059775095645	39.0	38.0	39.0	33.0	39.0
14-15	38.16253803276352	41.0	39.0	41.0	33.0	41.0
16-17	38.18119351270914	41.0	39.0	41.0	33.0	41.0
18-19	38.18945038203711	41.0	39.0	41.0	33.5	41.0
20-21	38.12654157316373	41.0	39.0	41.0	34.0	41.0
22-23	38.13112415357227	41.0	39.0	41.0	34.0	41.0
24-25	38.095783270509244	41.0	39.0	41.0	34.0	41.0
26-27	38.025592589068275	41.0	39.0	41.0	33.0	41.0
28-29	37.954755416396296	41.0	39.0	41.0	33.0	41.0
30-31	37.83797265705505	41.0	38.0	41.0	33.0	41.0
32-33	37.71661281630611	41.0	38.0	41.0	33.0	41.0
34-35	37.58550372521445	41.0	38.0	41.0	32.5	41.0
36-37	37.50896719678629	40.0	38.0	41.0	32.0	41.0
38-39	37.43068772417394	40.0	38.0	41.0	32.0	41.0
40-41	37.31529955926703	40.0	38.0	41.0	32.0	41.0
42-43	37.15718463588725	40.0	38.0	41.0	31.5	41.0
44-45	37.001523701410946	40.0	37.0	41.0	31.0	41.0
46-47	36.804194924756935	40.0	37.0	41.0	30.5	41.0
48-49	36.614784948421764	40.0	37.0	41.0	30.0	41.0
50-51	36.37175397565808	40.0	36.0	41.0	30.0	41.0
52-53	36.25051248297103	40.0	36.0	41.0	30.0	41.0
54-55	36.12196903440892	40.0	35.0	41.0	29.0	41.0
56-57	35.92253963942812	39.0	35.0	41.0	29.0	41.0
58-59	35.72923302326938	39.0	35.0	41.0	29.0	41.0
60-61	35.47365619959747	39.0	35.0	41.0	29.0	41.0
62-63	35.119505242938686	38.0	35.0	41.0	28.0	41.0
64-65	34.830860536253	37.0	35.0	40.0	28.0	41.0
66-67	34.527036196336326	37.0	35.0	39.5	28.0	41.0
68-69	34.1555428255014	36.0	35.0	39.0	28.0	41.0
70-71	33.781580403282376	35.5	34.5	39.0	27.0	41.0
72-73	33.46433037232061	35.0	34.0	37.5	26.5	40.0
74-75	33.14101637793724	35.0	34.0	37.0	26.0	39.0
76-77	32.816896660385055	35.0	34.0	36.5	26.0	39.0
78-79	32.555745523454675	35.0	34.0	36.0	26.0	38.5
80-81	32.3067868668558	35.0	34.0	36.0	26.0	37.0
82-83	32.082393681600195	35.0	34.0	35.0	25.5	37.0
84-85	31.87520017558549	35.0	34.0	35.0	25.0	36.5
86-87	31.749658942403464	35.0	34.0	35.0	25.0	36.0
88-89	31.653077838596197	35.0	34.0	35.0	25.0	36.0
90-91	31.548962625442968	35.0	33.5	35.0	25.0	36.0
92-93	31.469482697035176	35.0	33.0	35.0	24.0	35.0
94-95	31.401470098106955	35.0	33.0	35.0	24.0	35.0
96-97	31.340489525837892	35.0	33.0	35.0	24.0	35.0
98-99	31.264033091552626	35.0	33.0	35.0	23.5	35.0
100-101	31.19487526592451	35.0	33.0	35.0	23.0	35.0
102-103	30.506030013068496	34.0	31.5	35.0	20.5	35.0
104-105	31.025341069550883	35.0	33.0	35.0	22.5	35.0
106-107	31.12595222372422	35.0	33.0	35.0	23.0	35.0
108-109	31.10978324851324	35.0	33.0	35.0	23.0	35.0
110-111	31.044708072389803	35.0	33.0	35.0	20.0	35.0
112-113	31.007439909070435	35.0	33.0	35.0	20.0	35.0
114-115	30.884293132130473	35.0	33.0	35.0	19.0	35.0
116-117	31.066320341186703	35.0	33.0	35.0	20.0	35.0
118-119	31.04732033688314	35.0	33.0	35.0	19.5	35.0
120-121	30.96942794611362	35.0	33.0	35.0	18.5	35.0
122-123	30.835319508685792	35.0	33.0	35.0	8.5	35.0
124-125	30.196091501461538	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34717.0
3	9553.0
4	4975.0
5	4089.0
6	4284.0
7	4672.0
8	5047.0
9	5514.0
10	5922.0
11	6452.0
12	7092.0
13	8765.0
14	11338.0
15	10337.0
16	6058.0
17	5416.0
18	4801.0
19	4592.0
20	4292.0
21	4311.0
22	4859.0
23	6035.0
24	7932.0
25	10322.0
26	12421.0
27	14459.0
28	17496.0
29	21099.0
30	26001.0
31	32251.0
32	41949.0
33	57995.0
34	89467.0
35	159071.0
36	370012.0
37	780610.0
38	286612.0
39	471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02639746279785	17.712251776467173	11.052765750258153	27.208585010476817
2	15.95357048587069	19.70193988684312	37.25655778154373	27.087931845742464
3	18.8105660074113	23.507419672236225	28.585409580353115	29.09660473999936
4	13.385863354251464	14.992274245601397	34.26609164957549	37.35577075057165
5	14.570430595326291	37.34761237668015	32.740100334656105	15.34185669333744
6	34.67761461129533	33.924225676949504	16.50646684753743	14.891692864217735
7	30.39597058889663	31.587944107612987	19.40924232776186	18.60684297572852
8	28.381682434879284	34.35631775360256	18.49308285977668	18.76891695174147
9	28.10831960914402	15.139546664077288	18.237139641374135	38.51499408540456
10-11	26.89817803043171	24.927188681673748	26.32458704367626	21.850046244218277
12-13	26.880621315151522	22.223819664358537	26.806463851585562	24.089095168904386
14-15	24.234611797189558	23.560050610030768	24.196527485892418	28.008810106887257
16-17	24.439206909782257	26.643819363579734	24.835187493960063	24.081786232677942
18-19	24.399231670924397	25.847586032215574	26.551337579983453	23.201844716876572
20-21	25.06880767403158	24.871777027981988	26.432286528809783	23.62712876917665
22-23	24.924183447843184	24.47186634481492	26.294739177671346	24.30921102967055
24-25	24.441869100422554	24.730603048921466	26.338771761743324	24.48875608891265
26-27	24.264431099240326	24.974422843299955	26.471203552564855	24.289942504894864
28-29	24.266848444752508	25.360649140957474	26.342002142794463	24.03050027149555
30-31	24.7922054199215	24.921769201931095	26.278829674911826	24.007195703235578
32-33	24.486053622055586	24.92170980946139	26.3190375776177	24.27319899086533
34-35	24.546092499411223	25.091759406370258	26.36760428509434	23.994543809124178
36-37	24.551515574653994	25.06534669373944	26.33275693707876	24.050380794527808
38-39	24.550854537168938	25.2325763216145	26.24704183958964	23.969527301626922
40-41	24.62991893291559	24.949658837306828	26.305893514394153	24.114528715383425
42-43	24.48092501028108	24.96667017339161	26.102275226901046	24.45012958942627
44-45	24.32200313982853	25.06796346781293	26.16742946742942	24.442603924929116
46-47	24.323718179385907	25.087228812418545	26.014138802421865	24.57491420577368
48-49	24.367573372965122	25.30115354425498	25.869999791990484	24.46127329078941
50-51	24.385801492113373	25.381922494646776	25.91052675370183	24.32174925953802
52-53	24.51334079449675	25.280911368120908	25.733386857338363	24.472360980043977
54-55	24.22458605497015	25.117594555634486	25.88306798594771	24.774751403447656
56-57	24.359618978761517	25.294407788169366	25.718622782422873	24.627350450646247
58-59	24.34581192195863	25.27285381576937	25.918727589932555	24.46260667233944
60-61	24.575064200911736	25.24053973475179	25.95694085913145	24.227455205205025
62-63	24.45056140500203	25.3299070278314	25.880600840350198	24.338930726816372
64-65	24.304077533043017	25.021768870777784	25.898261789738292	24.77589180644091
66-67	24.31709268899633	25.21835890885938	25.785968938294285	24.678579463850006
68-69	24.228260095128295	25.211556935677226	25.897978320464826	24.662204648729656
70-71	24.13693181573661	25.10411043141335	26.017781377896597	24.74117637495344
72-73	24.256455291441792	25.31653922690717	25.72395115934278	24.703054322308265
74-75	24.095576065910702	25.327456624041872	25.79594518535592	24.781022124691503
76-77	24.128491080488395	25.01857733479083	25.74847292873476	25.10445865598601
78-79	23.976473822944	25.103315541263456	25.919630849416926	25.000579786375614
80-81	24.057339755528766	25.288996403653442	25.694894392884006	24.958769447933786
82-83	24.10427728532459	25.234885945673113	25.78768534141938	24.873151427582922
84-85	24.060285201334413	25.331146134661907	25.58589692001399	25.02267174398969
86-87	23.824755928042464	25.433548400053745	25.715192878650438	25.026502793253346
88-89	23.713997304026698	25.17351921108113	25.785707898608834	25.32677558628334
90-91	23.90714370590757	25.05180453355119	25.913217035831497	25.127834724709743
92-93	23.89115038619722	25.209380434746226	25.883103674336738	25.01636550471982
94-95	23.819316380197257	25.049665616330635	25.80730386415112	25.32371413932099
96-97	23.629673462800582	24.974244027232352	25.934118744060108	25.461963765906958
98-99	23.6260019887356	25.075665436239124	25.752667566754024	25.545665008271257
100-101	23.55740885166995	25.16557969749757	25.83007417913067	25.44693727170181
102-103	23.408957236511785	25.007770397892187	26.015172598468872	25.56809976712715
104-105	23.502466660514163	25.083548949953833	25.83755760203396	25.576426787498047
106-107	23.534317293061946	24.948404755369683	25.91379529646465	25.603482655103722
108-109	23.37319162488129	24.95705901086531	25.888204234612733	25.781545129640666
110-111	23.254973255690516	25.305457981356927	25.854163419063674	25.58540534388888
112-113	23.185270282709293	25.26951762853656	25.89755329564514	25.647658793109013
114-115	23.124302762554578	25.34214544235637	25.821108416866345	25.712443378222716
116-117	22.999477825266563	25.224649899055784	25.88265222684571	25.893220048831942
118-119	23.014968830775725	25.048804647756562	25.925913528564674	26.010312992903035
120-121	22.85244705386163	25.149110881687275	26.014224171527843	25.98421789292325
122-123	22.738607743535376	25.244313614665177	25.849951249649134	26.16712739215031
124-125	22.54696221721309	25.142850441320146	25.909984955049442	26.400202386417327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	72.0
2	59.5
3	101.0
4	105.0
5	104.0
6	236.0
7	529.5
8	885.0
9	1062.5
10	887.0
11	708.0
12	725.5
13	755.5
14	806.0
15	878.5
16	953.5
17	1029.0
18	1138.0
19	1195.5
20	1236.0
21	1342.5
22	1488.5
23	1752.0
24	2017.0
25	2354.0
26	3033.5
27	3968.0
28	5138.5
29	6727.5
30	8861.5
31	11196.5
32	14226.5
33	17676.5
34	21013.5
35	24772.5
36	29115.0
37	33505.5
38	37403.0
39	41836.5
40	46469.0
41	50498.0
42	54533.0
43	59992.5
44	66104.5
45	71685.0
46	76460.0
47	81221.0
48	86989.5
49	91674.5
50	94929.5
51	96814.0
52	95772.0
53	94422.0
54	93702.5
55	90627.0
56	85402.5
57	78253.0
58	70869.0
59	63207.5
60	54406.0
61	46214.0
62	38344.0
63	29497.0
64	21480.5
65	16264.5
66	12411.0
67	9551.5
68	7564.0
69	5886.5
70	4639.5
71	3628.0
72	2752.5
73	2275.0
74	1862.5
75	1399.0
76	1003.5
77	619.5
78	361.0
79	216.5
80	127.0
81	84.0
82	56.0
83	24.5
84	15.5
85	12.0
86	7.0
87	6.5
88	4.0
89	2.0
90	2.5
91	1.5
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023047986194160635
2	0.0015779741585213714
3	0.03337654432266415
4	0.16578292144223014
5	0.023239255789132923
6	0.06689654084155754
7	0.05059080787017002
8	0.04313129366625081
9	0.03337654432266415
10-11	0.06206698356850727
12-13	0.05460746936458805
14-15	0.056878795804883975
16-17	0.049491007699079374
18-19	0.03722584492148144
20-21	0.02378915587467825
22-23	0.0027255917283550957
24-25	0.004327474586248003
26-27	0.0032276744151573506
28-29	0.00451874418122029
30-31	0.0019605133484659463
32-33	0.009109214460555189
34-35	0.004136204991275715
36-37	0.006455348830314701
38-39	0.0019126959497228742
40-41	0.0027734091270981676
42-43	0.0032994005132719584
44-45	0.004399200684362611
46-47	0.0044709267824772185
48-49	0.0018409698516082666
50-51	0.0012910697660629402
52-53	7.411696805176138E-4
54-55	0.00475783117493565
56-57	6.933522817745419E-4
58-59	6.455348830314701E-4
60-61	1.1954349685767964E-4
62-63	8.607131773752934E-4
64-65	0.0
66-67	0.0030842222189281345
68-69	3.8253918994457487E-4
70-71	0.0
72-73	0.0018887872503513385
74-75	0.004590470279334898
76-77	0.0014823393610352276
78-79	7.172609811460778E-5
80-81	0.0
82-83	0.005475092156081727
84-85	0.0014106132629206198
86-87	0.0
88-89	0.0010519827723475808
90-91	6.933522817745419E-4
92-93	0.0
94-95	0.002558230832754344
96-97	0.0012671610666914043
98-99	5.020826868022544E-4
100-101	0.0
102-103	9.085305761183652E-4
104-105	0.0
106-107	5.259913861737904E-4
108-109	0.0022474177409243772
110-111	1.4345219622921557E-4
112-113	3.108130918299671E-4
114-115	0.0
116-117	0.001673608956007515
118-119	0.002080056845323626
120-121	0.0028690439245843114
122-123	0.0010519827723475808
124-125	0.10596335561464723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2091289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.96574244231525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07810337260182	49.620991288557406
2	11.09623711719304	13.751731425656851
3	3.599248378933843	6.690902941048058
4	1.6066036492484979	3.9821755174486486
5	0.939738648845274	2.9115801538717796
6	0.5816058329437921	2.162378234828595
7	0.3897429634689882	1.6905498465116804
8	0.28029071300133807	1.3894737704651055
9	0.2240261760811734	1.249375349464448
>10	1.1383577631756572	12.526170198348158
>50	0.05116666895379823	2.1175449201600185
>100	0.01418201577162994	1.3282442495478721
>500	4.644665207634671E-4	0.20799078582532415
>1k	2.3223326038173157E-4	0.3708913182660045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3350	0.1601882857892907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2647	0.12657265447291122	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.563479748614372E-5
2	0.0	0.0	0.0	0.0	9.563479748614372E-5
3	0.0	0.0	0.0	0.0	9.563479748614372E-5
4	0.0	0.0	0.0	0.0	9.563479748614372E-5
5	0.0	0.0	0.0	0.0	9.563479748614372E-5
6	0.0	0.0	0.0	0.0	9.563479748614372E-5
7	0.0	0.0	0.0	0.0	9.563479748614372E-5
8	0.0	0.0	0.0	0.0	9.563479748614372E-5
9	0.0	0.0	0.0	0.0	9.563479748614372E-5
10-11	0.0	0.0	0.0	0.0	9.563479748614372E-5
12-13	0.0	0.0	0.0	0.0	1.6736089560075152E-4
14-15	0.0	0.0	0.0	0.0	3.3472179120150303E-4
16-17	0.0	0.0	0.0	2.390869937153593E-5	3.8253918994457487E-4
18-19	0.0	0.0	0.0	4.781739874307186E-5	4.303565886876467E-4
20-21	0.0	0.0	0.0	1.4345219622921557E-4	4.5426528805918266E-4
22-23	0.0	0.0	0.0	4.0644788931611076E-4	5.499000855453263E-4
24-25	0.0	0.0	0.0	8.846218767468294E-4	6.216261836599342E-4
26-27	0.0	0.0	0.0	0.0015062480604067635	6.69443582403006E-4
28-29	0.0	0.0	0.0	0.0049251920705364015	6.69443582403006E-4
30-31	0.0	0.0	0.0	0.015205932800296851	6.69443582403006E-4
32-33	0.0	0.0	0.0	0.033902535708837944	7.172609811460778E-4
34-35	0.0	0.0	0.0	0.05840895256466227	7.172609811460778E-4
36-37	0.0	0.0	0.0	0.09288529705841708	7.650783798891497E-4
38-39	0.0	0.0	0.0	0.14596260966322683	7.650783798891497E-4
40-41	0.0	0.0	0.0	0.21271569830855516	7.650783798891497E-4
42-43	0.0	0.0	0.0	0.2764801995324415	7.650783798891497E-4
44-45	0.0	0.0	0.0	0.3472260409728163	7.650783798891497E-4
46-47	0.0	0.0	0.0	0.4247380443353358	8.128957786322215E-4
48-49	0.0	0.0	0.0	0.5060993482966725	9.085305761183653E-4
50-51	0.0	0.0	0.0	0.5922184834329449	9.085305761183653E-4
52-53	2.390869937153593E-5	0.0	0.0	0.6802024971201972	9.563479748614372E-4
54-55	4.781739874307186E-5	0.0	0.0	0.7818861955473395	0.001004165373604509
56-57	4.781739874307186E-5	0.0	0.0	0.8891884383267927	0.001004165373604509
58-59	4.781739874307186E-5	0.0	0.0	1.003543747420849	0.0010280740729760448
60-61	4.781739874307186E-5	0.0	0.0	1.1180186000117631	0.0010519827723475808
62-63	4.781739874307186E-5	0.0	0.0	1.2463604982381677	0.0010998001710906527
64-65	4.781739874307186E-5	0.0	0.0	1.3718333525399884	0.0010998001710906527
66-67	7.172609811460778E-5	0.0	0.0	1.518991397171792	0.0010998001710906527
68-69	9.563479748614372E-5	0.0	0.0	1.67788861319502	0.0010998001710906527
70-71	9.563479748614372E-5	0.0	0.0	1.8416871125894125	0.0011237088704621886
72-73	9.563479748614372E-5	0.0	0.0	1.9996518893371504	0.0012432523673198683
74-75	9.563479748614372E-5	0.0	0.0	2.1715793465178654	0.0012432523673198683
76-77	9.563479748614372E-5	0.0	0.0	2.3747793824765493	0.0012432523673198683
78-79	9.563479748614372E-5	0.0	0.0	2.59490677759028	0.0012671610666914043
80-81	9.563479748614372E-5	0.0	0.0	2.812332489675028	0.0013388871648060121
82-83	9.563479748614372E-5	0.0	0.0	3.040995290464398	0.0014345219622921557
84-85	9.563479748614372E-5	0.0	0.0	3.2860116416238982	0.0014345219622921557
86-87	9.563479748614372E-5	0.0	0.0	3.5513025698504608	0.0014345219622921557
88-89	9.563479748614372E-5	0.0	0.0	3.8518110122512956	0.0014823393610352276
90-91	9.563479748614372E-5	0.0	0.0	4.16491933922093	0.0014823393610352276
92-93	9.563479748614372E-5	0.0	0.0	4.471452773863392	0.001601882857892907
94-95	9.563479748614372E-5	0.0	0.0	4.793574680496096	0.001673608956007515
96-97	9.563479748614372E-5	0.0	0.0	5.153233245142111	0.001673608956007515
98-99	9.563479748614372E-5	0.0	0.0	5.554325585798997	0.001673608956007515
100-101	9.563479748614372E-5	0.0	0.0	5.984323543996071	0.001673608956007515
102-103	9.563479748614372E-5	0.0	0.0	6.4202747683366574	0.0017692437534936587
104-105	9.563479748614372E-5	0.0	0.0	6.868299885859869	0.0018170611522367306
106-107	9.563479748614372E-5	0.0	0.0	7.350012360797575	0.0018887872503513385
108-109	9.563479748614372E-5	0.0	0.0	7.872441350765007	0.0019605133484659463
110-111	9.563479748614372E-5	0.0	0.0	8.423010879892736	0.0020083307472090178
112-113	9.563479748614372E-5	0.0	0.0	8.970400552004051	0.0020561481459520897
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3240	0.0	50.08786	1
GTATCAA	5130	0.0	44.19517	1
GTACATG	10235	0.0	35.850555	1
TCAACGC	6535	0.0	34.4992	4
TACATGG	10500	0.0	34.426094	2
CAACGCA	6615	0.0	34.132042	5
TATCAAC	6855	0.0	32.891888	2
ATCAACG	6930	0.0	32.44984	3
ACATGGG	11000	0.0	32.373234	3
AACGCAG	7035	0.0	32.101223	6
CATGGGG	9180	0.0	31.770859	4
ATGGGGG	5405	0.0	29.8694	5
GTATAGG	540	0.0	27.621983	1
ACGCAGA	8310	0.0	27.174644	7
CGTATAC	135	8.1100254E-5	26.510746	3
CGCAGAG	8720	0.0	25.826134	8
GCAGAGT	9835	0.0	22.777054	9
TGGGGGG	3845	0.0	22.31581	6
GTACCGT	485	0.0	22.114508	6
AGAGTAC	8835	0.0	21.915981	10-11
>>END_MODULE