FastQCFastQC Report
Mon 15 Aug 2016
SRR2921633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670090
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.046.9930531
GTATCAA27650.041.548771
GTACATG85800.037.2548681
TACATGG89800.035.125092
TCAACGC32400.033.9816674
ACATGGG94900.033.237443
CAACGCA33950.032.6035655
CATGGGG84650.032.1297344
ATGGGGG49250.031.899925
ATCAACG35500.031.0142523
TATCACG1551.96771E-730.7168162
TATCAAC36600.030.5699482
AACGCAG36400.030.4063596
GCCTAGT4100.027.5795824
TATACCG2057.760718E-826.126465
CTAGTCT5050.024.744626
ACGCAGA46500.023.9241987
TGGGGGG36450.023.8346186
CCTAGTC4250.023.8041085
CGCAGAG48500.022.814298
Produced by FastQC (version 0.11.5)