##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921633_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1670090
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.35536108832458	31.0	31.0	34.0	27.0	34.0
2	30.340519971977557	31.0	31.0	34.0	27.0	34.0
3	30.558916585333723	31.0	31.0	34.0	27.0	34.0
4	34.04297073810393	37.0	35.0	37.0	30.0	37.0
5	34.08140878635283	37.0	35.0	37.0	30.0	37.0
6	33.990656790951384	37.0	35.0	37.0	30.0	37.0
7	34.03255632929962	37.0	35.0	37.0	30.0	37.0
8	34.03999724565742	37.0	35.0	37.0	30.0	37.0
9	35.69919824680107	39.0	37.0	39.0	30.0	39.0
10-11	35.72515193791951	39.0	37.0	39.0	30.0	39.0
12-13	35.660161428426015	39.0	37.0	39.0	30.0	39.0
14-15	36.97718805573352	40.5	38.0	41.0	30.5	41.0
16-17	36.932333287427625	40.5	38.0	41.0	30.0	41.0
18-19	36.873246351993004	40.0	38.0	41.0	30.0	41.0
20-21	36.938594626636885	40.0	38.0	41.0	30.0	41.0
22-23	36.86086648024957	40.0	38.0	41.0	30.0	41.0
24-25	36.84166003029777	40.0	38.0	41.0	30.0	41.0
26-27	36.801336754306654	40.0	38.0	41.0	30.0	41.0
28-29	36.75268398709052	40.0	38.0	41.0	30.0	41.0
30-31	36.65981863252878	40.0	38.0	41.0	30.0	41.0
32-33	36.57521570693795	40.0	38.0	41.0	30.0	41.0
34-35	36.44867731679131	40.0	37.5	41.0	29.5	41.0
36-37	36.347263321138385	40.0	37.0	41.0	29.0	41.0
38-39	36.2298894071577	40.0	37.0	41.0	28.0	41.0
40-41	36.12105874533708	40.0	37.0	41.0	28.0	41.0
42-43	35.873560706309235	40.0	36.0	41.0	27.0	41.0
44-45	35.501448424935184	40.0	35.0	41.0	26.0	41.0
46-47	35.249392847092075	39.0	35.0	41.0	25.5	41.0
48-49	35.05076852145693	39.0	35.0	41.0	24.5	41.0
50-51	34.847385170859056	39.0	35.0	41.0	24.0	41.0
52-53	34.637747666293436	39.0	34.5	40.0	24.0	41.0
54-55	34.39021220413271	38.0	34.0	40.0	23.0	41.0
56-57	34.12851762479866	38.0	34.0	40.0	23.0	41.0
58-59	34.129825638139266	38.0	34.0	40.0	23.0	41.0
60-61	33.912428970893785	37.0	34.0	40.0	22.5	41.0
62-63	33.59934494548198	37.0	34.0	40.0	22.0	41.0
64-65	33.29395002664527	36.0	33.5	39.5	21.5	41.0
66-67	32.967284996616954	36.0	33.0	39.0	20.5	41.0
68-69	32.62778622708956	35.0	33.0	39.0	20.0	40.5
70-71	32.265791364537236	35.0	33.0	37.5	19.0	40.0
72-73	31.912917567316732	35.0	33.0	37.0	18.0	39.0
74-75	31.548553071990135	35.0	32.0	36.5	16.5	39.0
76-77	31.25563203180667	35.0	32.0	36.0	15.0	38.5
78-79	30.990071493153064	35.0	32.0	36.0	12.5	37.0
80-81	30.733963139711033	35.0	32.0	35.0	10.0	37.0
82-83	30.51706554736571	35.0	31.5	35.0	7.5	36.5
84-85	30.307212186169608	35.0	31.0	35.0	7.0	36.0
86-87	30.139877491632188	35.0	31.0	35.0	6.5	36.0
88-89	29.976463543880868	35.0	31.0	35.0	3.5	35.5
90-91	29.79799591638774	34.0	31.0	35.0	2.0	35.0
92-93	29.71823554419223	34.0	31.0	35.0	2.0	35.0
94-95	29.5898709051608	34.0	31.0	35.0	2.0	35.0
96-97	29.500427222485015	34.0	31.0	35.0	2.0	35.0
98-99	29.400840074487004	34.0	30.0	35.0	2.0	35.0
100-101	29.28432030609129	34.0	30.0	35.0	2.0	35.0
102-103	28.371610512008335	33.5	29.0	34.5	2.0	35.0
104-105	29.32192516570963	34.0	30.5	35.0	2.0	35.0
106-107	29.612295744540713	35.0	31.0	35.0	2.0	35.0
108-109	29.606194276955137	35.0	31.0	35.0	2.0	35.0
110-111	29.535243310240766	35.0	31.0	35.0	2.0	35.0
112-113	29.442401008328893	35.0	31.0	35.0	2.0	35.0
114-115	29.312885533114983	35.0	31.0	35.0	2.0	35.0
116-117	29.184623882545253	35.0	31.0	35.0	2.0	35.0
118-119	28.889988264105526	34.0	31.0	35.0	2.0	35.0
120-121	28.745581375853995	34.0	30.0	35.0	2.0	35.0
122-123	28.47681651887024	34.0	30.0	35.0	2.0	35.0
124-125	27.670535719631875	33.5	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59831.0
3	10914.0
4	5477.0
5	4781.0
6	5062.0
7	5360.0
8	5661.0
9	5789.0
10	6405.0
11	6469.0
12	6297.0
13	6373.0
14	6095.0
15	5814.0
16	5410.0
17	4927.0
18	4683.0
19	4558.0
20	4528.0
21	4731.0
22	5407.0
23	7074.0
24	9957.0
25	12484.0
26	14295.0
27	16420.0
28	19692.0
29	24212.0
30	29088.0
31	36921.0
32	48671.0
33	66632.0
34	103410.0
35	169771.0
36	333763.0
37	486398.0
38	116680.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.004515889462525	16.781859779835415	11.381299186660758	25.832325144041302
2	17.236815708866178	19.599433493923236	37.23238426598239	25.9313665312282
3	20.513898420386344	22.858407259827224	28.157340969709598	28.47035335007683
4	14.667582504777311	14.550088539667389	33.51961496473085	37.26271399082445
5	15.26222364887687	36.76335312816656	32.16033575358906	15.814087469367516
6	35.14291536557445	32.383081505810715	16.622054936558644	15.851948192056195
7	30.88163134721188	30.79421891691266	19.522329112892645	18.801820622982817
8	28.11174366436643	34.34011342825426	18.67540432853337	18.872738578845937
9	28.253641976750522	15.731219330761503	17.83917265452385	38.17596603796412
10-11	27.130319133003038	24.73894836479532	26.0534923706424	22.07724013155924
12-13	26.823810730063258	22.286927896739208	26.2368485192551	24.652412853942433
14-15	24.562382307703366	23.645969592862517	23.330087062352362	28.461561037081758
16-17	25.20545270275127	26.65326532030256	23.965848130320506	24.175433846625662
18-19	25.112160945078205	26.0156484698219	25.226845305503982	23.645345279595915
20-21	25.66657881969609	24.939385590301693	25.073491976976108	24.320543613026107
22-23	25.881818064739885	24.509077774539822	24.85072040861741	24.758383752102883
24-25	25.29683816850543	24.993853150645876	24.821471835750195	24.887836845098498
26-27	24.840989833233643	25.288689378824742	25.076266472734414	24.794054315207198
28-29	24.978868476559434	25.574415011738406	24.97266829888091	24.47404821282125
30-31	25.548352397346015	25.02213054566319	25.012098430917362	24.417418626073438
32-33	25.022685522653475	25.11678177635279	24.98599936390693	24.874533337086802
34-35	25.05243893855142	25.46847598352026	25.000711366437585	24.47837371149074
36-37	25.098694049793004	25.561317262034688	24.886709560590287	24.453279127582025
38-39	25.10203024389338	25.551984442951852	25.039834391940474	24.306150921214297
40-41	25.07661637182243	25.29289738078636	25.101835677950056	24.528650569441147
42-43	25.057674893274495	25.125381504745754	24.925136431367477	24.891807170612275
44-45	24.788059973218544	25.218767142648396	25.21996488227417	24.773208001858894
46-47	24.749391427307717	25.266135448861476	25.147610133764715	24.836862990066095
48-49	24.660120382235608	25.486935931003774	25.25876730243828	24.59417638432234
50-51	24.691865279310495	25.333664749543676	25.407215190442034	24.56725478070379
52-53	24.760333013807298	25.296070668258608	25.295052497199283	24.648543820734815
54-55	24.6879756651114	24.967606485350824	25.333330538422587	25.011087311115187
56-57	24.729480042284184	25.274622601675194	25.036459627887197	24.95943772815342
58-59	24.629660959852675	25.4413510701212	25.284953923777724	24.6440340462484
60-61	24.892973888503587	25.269661481260545	25.307752475615175	24.52961215462069
62-63	24.774301042940596	25.242168961886613	25.373270200311193	24.610259794861598
64-65	24.681527947174708	24.974179964970393	25.28794317406312	25.056348913791776
66-67	24.652215123060728	25.336000634881657	25.071596382971396	24.94018785908622
68-69	24.49038810518284	25.31155260040414	25.325836586408833	24.872222708004184
70-71	24.469230971990253	25.091207205998288	25.59034575468646	24.849216067324996
72-73	24.580856362227305	25.35044923861212	25.263488238105445	24.805206161055125
74-75	24.46603156847416	25.312057334294252	25.265678019767947	24.956233077463637
76-77	24.492841587274306	24.976816342614484	25.33796337745689	25.192378692654323
78-79	24.37248733655054	25.14892935270046	25.49040452664977	24.98817878409923
80-81	24.362679778292154	25.387973756786135	25.335601117522526	24.913745347399185
82-83	24.50259453399007	25.30560040822585	25.40418311708224	24.787621940701843
84-85	24.530663366636055	25.237119835006457	25.262690481102425	24.969526317255067
86-87	24.3043761034282	25.402534201782736	25.331956718066156	24.961132976722904
88-89	24.2501547438768	25.185745056955028	25.411774429394118	25.152325769774052
90-91	24.449895761527767	25.08769387128091	25.404362025214873	25.058048341976452
92-93	24.359105254916237	25.237274997073268	25.541685217615733	24.861934530394763
94-95	24.440176058446614	24.932061800107792	25.574585304509252	25.05317683693634
96-97	24.25716722241386	25.049196801579086	25.54853086313288	25.145105112874173
98-99	24.191131405535963	25.142824293708006	25.380013970298293	25.286030330457738
100-101	24.156198556451745	25.20706168524947	25.501208745605307	25.135531012693473
102-103	23.941357448072353	25.05203542960067	25.75080118992872	25.25580593239825
104-105	23.873647841486452	25.15920515683991	25.73860115028907	25.22854585138457
106-107	23.963742902256367	25.050761254148775	25.73136024592937	25.25413559766549
108-109	23.950294564590866	25.101694027113417	25.744301466460062	25.203709941835655
110-111	23.784004521960732	25.39534223674189	25.63874491638684	25.18190832491054
112-113	23.655093704230193	25.481844169188562	25.774261964669154	25.088800161912094
114-115	23.601383473001107	25.489121663521185	25.73806535954758	25.17142950393013
116-117	23.576818066547446	25.28977117411181	25.809687113300356	25.323723646040392
118-119	23.64516934782713	25.200876881175517	25.794200719566145	25.359753051431206
120-121	23.499768721922916	25.473820668925057	25.869193909826997	25.157216699325026
122-123	23.439460049494663	25.471519610748956	25.837250812686108	25.251769527070277
124-125	23.329805521885845	25.27278471660782	25.959086196726943	25.438323564779385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	392.0
1	310.5
2	208.0
3	166.0
4	140.5
5	130.0
6	122.5
7	129.0
8	147.0
9	162.5
10	170.0
11	195.0
12	215.0
13	226.0
14	260.5
15	283.5
16	327.5
17	345.5
18	361.5
19	420.5
20	451.0
21	526.5
22	674.5
23	812.5
24	968.5
25	1203.0
26	1550.0
27	2160.0
28	3052.0
29	4224.0
30	5646.0
31	7315.0
32	9664.0
33	12096.5
34	14602.5
35	17623.5
36	20985.5
37	24070.5
38	27414.0
39	31378.0
40	35211.5
41	38588.0
42	42246.5
43	47683.0
44	52690.0
45	57458.5
46	61682.5
47	65762.0
48	71301.5
49	75962.0
50	78216.5
51	78204.5
52	77125.5
53	75932.0
54	75262.5
55	73393.5
56	69028.0
57	63623.0
58	58485.5
59	52796.5
60	45450.0
61	38308.5
62	32096.5
63	25372.0
64	19207.0
65	14790.0
66	11554.0
67	9227.0
68	7420.0
69	5826.0
70	4588.5
71	3561.5
72	2733.5
73	2185.5
74	1739.5
75	1340.5
76	983.5
77	632.5
78	396.5
79	276.0
80	191.5
81	125.5
82	90.0
83	65.0
84	60.5
85	43.5
86	32.5
87	32.5
88	27.0
89	23.5
90	21.5
91	15.5
92	12.5
93	13.5
94	13.5
95	10.5
96	8.5
97	6.0
98	5.0
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02574711542491722
2	0.012274787586297745
3	0.011256878371824272
4	0.012993311737690782
5	0.017603841709129448
6	0.09700076043806023
7	0.05933811950254178
8	0.0503565676101288
9	0.0276033028160159
10-11	0.05269177110215617
12-13	0.013232819788155128
14-15	0.028860720080953722
16-17	0.036285469645348456
18-19	0.0434407726529708
20-21	0.03113604656036501
22-23	0.039249381769844736
24-25	0.03206414025591435
26-27	0.04661425432162338
28-29	0.04688370087839578
30-31	0.027064409702471124
32-33	0.03131567759821327
34-35	0.04595560718284643
36-37	0.009370752474417546
38-39	0.02182517109856355
40-41	0.04394972726020754
42-43	0.023112526869809413
44-45	0.016645809507272062
46-47	0.024220251603207014
48-49	0.029429551700806546
50-51	0.0291900436503422
52-53	0.025717176918609176
54-55	0.023262219401349628
56-57	0.02604650048799765
58-59	0.018112796316366184
60-61	0.02419031309689897
62-63	0.023232280895041584
64-65	0.021525786035483115
66-67	0.0291900436503422
68-69	0.023232280895041584
70-71	0.04873988826949446
72-73	0.022513756743648545
74-75	0.009849768575346238
76-77	0.0162566089252675
78-79	0.02529803783029657
80-81	0.01946002910022813
82-83	0.025507607374452873
84-85	0.012364603105221875
86-87	0.017603841709129448
88-89	0.023681358489662234
90-91	0.021615601554407247
92-93	0.008352843259944074
94-95	0.011376632397056446
96-97	0.015927285355879024
98-99	0.007095425995006257
100-101	0.01059823123304732
102-103	0.004430898933590405
104-105	0.004490775946206492
106-107	0.0010179092144734715
108-109	0.015118945685561856
110-111	0.001077786227089558
112-113	0.002874096605572155
114-115	0.0140411594584723
116-117	0.006496655868845391
118-119	0.2779490925638738
120-121	0.0013472327838619476
122-123	6.586471387769521E-4
124-125	0.3127975139064362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1670090.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.30703580454394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.76305014541657	54.05009479139986
2	9.472892181209618	12.37293017701682
3	3.0711638102038856	6.017058147438143
4	1.4734598608872123	3.8490918356607806
5	0.8422823970987129	2.7503483332431373
6	0.5039887372088084	1.9748406303589525
7	0.3596810256762442	1.64427911354375
8	0.2647214457177502	1.38305383469758
9	0.20187311166065203	1.186536107807721
>10	0.9861920025604944	11.091836504742044
>50	0.04642384446925381	2.0708879442271244
>100	0.013905588313088671	1.4545175031397117
>500	3.658495779192495E-4	0.15452507672438506
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.987701261608656E-5
10-11	0.0	0.0	0.0	5.987701261608656E-5	5.987701261608656E-5
12-13	0.0	0.0	0.0	5.987701261608656E-5	5.987701261608656E-5
14-15	0.0	0.0	0.0	5.987701261608656E-5	1.1975402523217311E-4
16-17	0.0	0.0	0.0	8.981551892412983E-5	1.7963103784825968E-4
18-19	0.0	0.0	0.0	1.1975402523217311E-4	2.0956954415630297E-4
20-21	0.0	0.0	0.0	1.496925315402164E-4	2.3950805046434623E-4
22-23	0.0	0.0	0.0	5.089546072367358E-4	3.892005820045626E-4
24-25	0.0	0.0	0.0	8.682166829332551E-4	4.7901610092869246E-4
26-27	0.0	0.0	0.0	0.0013771712901699909	5.38893113544779E-4
28-29	0.0	0.0	0.0	0.004879976528211054	5.38893113544779E-4
30-31	0.0	0.0	0.0	0.01910076702453161	5.987701261608656E-4
32-33	0.0	0.0	0.0	0.046075361208078605	6.586471387769521E-4
34-35	0.0	5.987701261608656E-5	0.0	0.07909753366585034	7.185241513930387E-4
36-37	0.0	5.987701261608656E-5	0.0	0.12292750690082571	7.784011640091253E-4
38-39	0.0	5.987701261608656E-5	0.0	0.18723541845050268	7.784011640091253E-4
40-41	0.0	5.987701261608656E-5	0.0	0.2630097779161602	7.784011640091253E-4
42-43	0.0	5.987701261608656E-5	0.0	0.34174804950631404	7.784011640091253E-4
44-45	0.0	5.987701261608656E-5	0.0	0.4331802477710782	7.784011640091253E-4
46-47	0.0	5.987701261608656E-5	0.0	0.5281152512738835	7.784011640091253E-4
48-49	0.0	5.987701261608656E-5	0.0	0.6266428755336539	7.784011640091253E-4
50-51	0.0	5.987701261608656E-5	0.0	0.7245118526546473	7.784011640091253E-4
52-53	0.0	5.987701261608656E-5	0.0	0.8265422821524588	9.580322018573849E-4
54-55	0.0	5.987701261608656E-5	0.0	0.9487213263955835	0.0011077247333976014
56-57	0.0	5.987701261608656E-5	0.0	1.078474812734643	0.0011676017460136878
58-59	0.0	5.987701261608656E-5	0.0	1.2165212653210307	0.0012274787586297746
60-61	0.0	5.987701261608656E-5	0.0	1.3500170649485956	0.0012574172649378178
62-63	0.0	5.987701261608656E-5	0.0	1.4926740475064217	0.0012574172649378178
64-65	0.0	5.987701261608656E-5	0.0	1.647785448688394	0.0012574172649378178
66-67	0.0	5.987701261608656E-5	0.0	1.8287337808142077	0.001287355771245861
68-69	0.0	5.987701261608656E-5	0.0	2.0242022884994224	0.0013172942775539042
70-71	0.0	5.987701261608656E-5	0.0	2.2172158386673773	0.0013771712901699909
72-73	0.0	5.987701261608656E-5	0.0	2.4048105191935765	0.0015568023280182505
74-75	0.0	5.987701261608656E-5	0.0	2.6109071966181463	0.0015568023280182505
76-77	0.0	5.987701261608656E-5	0.0	2.863169050769719	0.001586740834326294
78-79	0.0	5.987701261608656E-5	0.0	3.1338730248070465	0.0016166793406343371
80-81	0.0	5.987701261608656E-5	0.0	3.3871827266794003	0.0016765563532504238
82-83	0.0	5.987701261608656E-5	0.0	3.6413905837409963	0.0017364333658665102
84-85	0.0	5.987701261608656E-5	0.0	3.9163458256740658	0.0017364333658665102
86-87	0.0	5.987701261608656E-5	0.0	4.240549910483866	0.0017364333658665102
88-89	0.0	5.987701261608656E-5	0.0	4.6065481500996945	0.0017364333658665102
90-91	0.0	5.987701261608656E-5	0.0	4.971378787969511	0.0017364333658665102
92-93	0.0	5.987701261608656E-5	0.0	5.3211204186600725	0.0017364333658665102
94-95	0.0	8.981551892412983E-5	0.0	5.68361585303786	0.0018861258974067264
96-97	0.0	1.1975402523217311E-4	0.0	6.106197869575891	0.0019759414163308562
98-99	0.0	1.1975402523217311E-4	0.0	6.5942553994096125	0.0019759414163308562
100-101	0.0	1.1975402523217311E-4	0.0	7.095815195588262	0.0019759414163308562
102-103	2.993850630804328E-5	1.1975402523217311E-4	0.0	7.586567190989707	0.002035818428946943
104-105	5.987701261608656E-5	1.1975402523217311E-4	0.0	8.088725757294517	0.0020657569352549865
106-107	5.987701261608656E-5	1.7963103784825968E-4	0.0	8.645581974624122	0.0021256339478710725
108-109	5.987701261608656E-5	1.7963103784825968E-4	0.0	9.260249447634559	0.002155572454179116
110-111	5.987701261608656E-5	1.7963103784825968E-4	0.0	9.912369992036357	0.002155572454179116
112-113	5.987701261608656E-5	1.7963103784825968E-4	0.0	10.534941230712118	0.002155572454179116
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1900	0.0	46.993053	1
GTATCAA	2765	0.0	41.54877	1
GTACATG	8580	0.0	37.254868	1
TACATGG	8980	0.0	35.12509	2
TCAACGC	3240	0.0	33.981667	4
ACATGGG	9490	0.0	33.23744	3
CAACGCA	3395	0.0	32.603565	5
CATGGGG	8465	0.0	32.129734	4
ATGGGGG	4925	0.0	31.89992	5
ATCAACG	3550	0.0	31.014252	3
TATCACG	155	1.96771E-7	30.716816	2
TATCAAC	3660	0.0	30.569948	2
AACGCAG	3640	0.0	30.406359	6
GCCTAGT	410	0.0	27.579582	4
TATACCG	205	7.760718E-8	26.12646	5
CTAGTCT	505	0.0	24.74462	6
ACGCAGA	4650	0.0	23.924198	7
TGGGGGG	3645	0.0	23.834618	6
CCTAGTC	425	0.0	23.804108	5
CGCAGAG	4850	0.0	22.81429	8
>>END_MODULE