FastQCFastQC Report
Mon 15 Aug 2016
SRR2921633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670090
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.11741882174014573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.051.8016131
GTATCAA38050.048.1278721
TCAACGC48350.037.3727264
GTACATG85850.037.0339361
CAACGCA49850.036.0906225
ATCAACG50400.035.854763
TACATGG88350.035.5069922
TATCAAC51150.035.3290252
AACGCAG52400.034.3549046
ACATGGG93050.033.264863
TATCACG1805.875336E-1033.133112
CATGGGG82500.031.5890184
TATACCG2151.200533E-1030.479435
ACGCAGA61650.029.200287
ATGGGGG44350.029.1486665
CGCAGAG67550.026.7356838
GTGTACG2651.3405952E-924.7605171
GCAGAGT76750.023.0642369
GTGGTAT14750.022.6469081
TGGGGGG29200.022.251376
Produced by FastQC (version 0.11.5)