##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921633_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1670090 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923554419222917 33.0 31.0 34.0 30.0 34.0 2 32.0906112844218 34.0 31.0 34.0 30.0 34.0 3 32.16126915315941 34.0 31.0 34.0 30.0 34.0 4 35.56834901113114 37.0 35.0 37.0 35.0 37.0 5 35.44761958936345 37.0 35.0 37.0 33.0 37.0 6 35.49110287469538 37.0 36.0 37.0 35.0 37.0 7 35.39203216593118 37.0 36.0 37.0 33.0 37.0 8 35.42701291547162 37.0 36.0 37.0 33.0 37.0 9 37.171667993940446 39.0 38.0 39.0 35.0 39.0 10-11 37.12574681603985 39.0 38.0 39.0 34.5 39.0 12-13 37.06419055260495 39.0 38.0 39.0 34.0 39.0 14-15 38.518116987707245 41.0 39.0 41.0 34.5 41.0 16-17 38.52504595560718 41.0 39.0 41.0 35.0 41.0 18-19 38.5268784915783 41.0 39.0 41.0 34.5 41.0 20-21 38.463065463537895 41.0 39.0 41.0 34.0 41.0 22-23 38.46702542976726 41.0 39.0 41.0 34.0 41.0 24-25 38.43016304510535 41.0 39.0 41.0 34.0 41.0 26-27 38.362647222604764 41.0 39.0 41.0 34.0 41.0 28-29 38.295402942356404 41.0 39.0 41.0 34.0 41.0 30-31 38.18970145321509 41.0 38.5 41.0 34.0 41.0 32-33 38.082636564496525 41.0 38.0 41.0 34.0 41.0 34-35 37.95679663970205 41.0 38.0 41.0 33.0 41.0 36-37 37.88770215976385 41.0 38.0 41.0 33.0 41.0 38-39 37.809585710949705 40.5 38.0 41.0 33.0 41.0 40-41 37.69547599231179 40.0 38.0 41.0 33.0 41.0 42-43 37.54163488195247 40.0 38.0 41.0 33.0 41.0 44-45 37.396982198564146 40.0 37.5 41.0 32.5 41.0 46-47 37.21268314881234 40.0 37.0 41.0 32.0 41.0 48-49 37.030704333299404 40.0 37.0 41.0 31.5 41.0 50-51 36.786085181038146 40.0 36.0 41.0 31.0 41.0 52-53 36.65788220994078 40.0 36.0 41.0 31.0 41.0 54-55 36.524286116317086 40.0 35.5 41.0 31.0 41.0 56-57 36.3232691052578 39.0 35.0 41.0 31.0 41.0 58-59 36.12236885437312 39.0 35.0 41.0 31.0 41.0 60-61 35.869600141309746 39.0 35.0 41.0 30.0 41.0 62-63 35.512607703776446 37.5 35.0 41.0 30.0 41.0 64-65 35.22357118478645 37.0 35.0 40.0 30.0 41.0 66-67 34.91321006652336 37.0 35.0 39.5 29.5 41.0 68-69 34.54026699159925 36.0 35.0 39.0 29.0 41.0 70-71 34.1652135513655 35.5 35.0 39.0 29.0 41.0 72-73 33.846485818129565 35.0 35.0 37.5 29.0 40.0 74-75 33.52854576699459 35.0 34.0 37.0 29.0 39.0 76-77 33.20775856390973 35.0 34.0 36.5 28.0 39.0 78-79 32.9520256393368 35.0 34.0 36.0 28.0 38.0 80-81 32.71005933811951 35.0 34.0 36.0 28.0 37.0 82-83 32.496446898071355 35.0 34.0 35.0 27.0 37.0 84-85 32.29355543713213 35.0 34.0 35.0 27.0 36.0 86-87 32.17300893963798 35.0 34.0 35.0 27.0 36.0 88-89 32.08451670269267 35.0 34.0 35.0 27.0 36.0 90-91 31.989700255674844 35.0 34.0 35.0 26.5 35.5 92-93 31.9146093923082 35.0 34.0 35.0 26.0 35.0 94-95 31.852905532037198 35.0 34.0 35.0 26.0 35.0 96-97 31.79065439587088 35.0 34.0 35.0 25.5 35.0 98-99 31.714686933039538 35.0 33.5 35.0 25.0 35.0 100-101 31.65256303552503 35.0 33.0 35.0 25.0 35.0 102-103 30.9617736169907 34.0 32.0 35.0 24.5 35.0 104-105 31.485858546545394 35.0 33.0 35.0 25.0 35.0 106-107 31.579259201599914 35.0 33.0 35.0 25.0 35.0 108-109 31.56337442892299 35.0 33.0 35.0 25.0 35.0 110-111 31.497410319204356 35.0 33.0 35.0 25.0 35.0 112-113 31.456627786526475 35.0 33.0 35.0 25.0 35.0 114-115 31.328492476453363 35.0 33.0 35.0 24.0 35.0 116-117 31.512542737217753 35.0 34.0 35.0 25.0 35.0 118-119 31.48736774664838 35.0 34.0 35.0 25.0 35.0 120-121 31.406024525624368 35.0 34.0 35.0 24.5 35.0 122-123 31.267621505427854 35.0 34.0 35.0 24.0 35.0 124-125 30.629921141974386 34.5 32.5 35.0 18.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20878.0 3 5541.0 4 3093.0 5 2571.0 6 2638.0 7 2961.0 8 3270.0 9 3623.0 10 3928.0 11 4236.0 12 4838.0 13 5935.0 14 7933.0 15 7170.0 16 4269.0 17 3834.0 18 3461.0 19 3164.0 20 3093.0 21 3200.0 22 3683.0 23 4350.0 24 5864.0 25 7462.0 26 9256.0 27 11218.0 28 14019.0 29 16451.0 30 20596.0 31 25838.0 32 34305.0 33 46415.0 34 73020.0 35 130569.0 36 306433.0 37 636829.0 38 223851.0 39 295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68116072230707 17.173479471745576 10.758721887820801 27.386637918126556 2 16.645779231082898 19.591356733248986 36.51305369917183 27.24981033649629 3 19.616677218355267 22.88053312281866 28.092628053276343 29.410161605549728 4 14.004318359023571 14.598392610807892 33.34600851676363 38.051280513404905 5 15.200729719353914 36.707985887141454 32.29499252253272 15.796291870971915 6 35.550695372373255 32.65266134975942 16.29753908286346 15.499104195003866 7 31.100846528514168 30.98150577832096 18.914190885290296 19.00345680787457 8 28.873093892368377 33.56822150535702 18.42030066404881 19.13838393822579 9 28.46687347224327 15.04501585183313 18.009754882083087 38.47835579384052 10-11 27.439416212336315 24.364099778823828 25.90349553019221 22.292988478647647 12-13 27.26952197649949 21.80353331486044 26.391420590283758 24.53552411835631 14-15 24.687710351219696 23.210941813276566 23.681379631396513 28.419968204107228 16-17 24.89710005011327 26.45815991135972 24.375928016613763 24.26881202191325 18-19 24.86755324899989 25.490245407978524 26.154687981541162 23.48751336148042 20-21 25.51924837977257 24.457303915197766 25.934030196331857 24.08941750869781 22-23 25.411944342567157 24.07739347170348 25.853970344977284 24.656691840752078 24-25 24.807554411854966 24.56629945494583 25.831106560260597 24.795039572938606 26-27 24.557464629504995 24.822129582835338 26.072342153103286 24.54806363455638 28-29 24.65321388120515 25.171024066571917 25.939940006473 24.23582204574993 30-31 25.207328873591962 24.627346932909873 25.906977746800695 24.25834644669747 32-33 24.791429881995903 24.57854823700464 25.91664860216702 24.71337327883244 34-35 24.831558298313666 24.852905424744943 25.973165494421004 24.342370782520387 36-37 24.746382528471056 25.02464841760602 25.924775993564015 24.30419306035891 38-39 24.840087362523033 25.031967557906114 26.033642654946476 24.094302424624374 40-41 24.84775015126933 24.70245375170393 26.049162457422288 24.40063363960445 42-43 24.793753396276408 24.64450435696418 25.776551583731404 24.785190663028004 44-45 24.612166773302405 24.785668156972196 25.87508918330399 24.727075886421414 46-47 24.623693612424553 24.888271995080284 25.674941340364803 24.813093052130355 48-49 24.528370238195034 25.15029400141312 25.72748883273655 24.593846927655292 50-51 24.59741848448459 25.10200947208358 25.746355457315893 24.554216586115935 52-53 24.745108523666772 25.045574341488564 25.560791186735194 24.64852594810947 54-55 24.54015080203401 24.737485658716984 25.66736367098522 25.05499986826379 56-57 24.604711221312318 25.01071058720963 25.439463401826202 24.94511478965185 58-59 24.491101003786653 25.12661100452914 25.704399081239416 24.677888910444796 60-61 24.75656230193789 24.954545821972907 25.803991824385353 24.484900051703846 62-63 24.59245891057718 25.018696742725037 25.75596813799603 24.632876208701752 64-65 24.505924830398364 24.73082288978438 25.70547096264273 25.05778131717452 66-67 24.44646568557299 25.101150211282235 25.542816014696644 24.90956808844813 68-69 24.393068360157383 25.09291465503769 25.665970692439465 24.84804629236546 70-71 24.284349945212533 24.945002963912124 25.900131130657627 24.870515960217713 72-73 24.518610109816414 25.117420930984512 25.564620432798822 24.79934852640025 74-75 24.33698968662267 25.115425575391363 25.576229681700557 24.971355056285404 76-77 24.336910845226107 24.832730760893906 25.58695425678807 25.243404137091918 78-79 24.222143117822412 24.9747991944125 25.749310439495467 25.053747248269627 80-81 24.27021897023514 25.213042410888036 25.46407678628098 25.052661832595845 82-83 24.35333948501889 25.147020693974802 25.5415708834056 24.95806893760071 84-85 24.36714194828038 25.12325137532278 25.381864451180718 25.12774222521612 86-87 24.141902532198863 25.261632606625987 25.427911070660862 25.168553790514288 88-89 24.02148650521849 25.04949638383746 25.54998393781344 25.379033173130612 90-91 24.142300025687437 24.991961448484112 25.607322297115644 25.25841622871281 92-93 24.10645534073014 25.0476321635361 25.706997826464438 25.13891466926932 94-95 24.08156336468789 24.842294102113783 25.686795338312457 25.389347194885868 96-97 23.93303234614325 24.91781732392848 25.63186352586135 25.51728680406692 98-99 23.899267131135296 24.967898254006318 25.515386833149662 25.617447781708723 100-101 23.81422558065733 25.03853085761845 25.65197085187026 25.49527270985396 102-103 23.655195125967396 24.926934419112914 25.855096328009218 25.56277412691047 104-105 23.747522588603008 24.914824949553616 25.764300127538036 25.573352334305337 106-107 23.767871919754647 24.924434667123332 25.70227258846593 25.605420824656093 108-109 23.771954406210106 24.930361449823117 25.67955967838127 25.618124465585502 110-111 23.551027385674473 25.229261802593093 25.627923310650903 25.59178750108153 112-113 23.499911681141977 25.237907052635045 25.691596535505678 25.5705847307173 114-115 23.425474076247387 25.270733912543637 25.643917393673394 25.659874617535582 116-117 23.323434478492818 25.11250354403753 25.749427340419512 25.814634637050137 118-119 23.421553167671775 25.07410699610162 25.665705518662147 25.838634317564456 120-121 23.259426875659788 25.180759251730052 25.737482777348315 25.82233109526185 122-123 23.1787776115502 25.27713716789122 25.625326895665534 25.918758324893048 124-125 22.983699722679198 25.220728239098307 25.655546161341185 26.14002587688131 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 7.0 2 11.5 3 38.5 4 42.0 5 45.0 6 135.5 7 317.0 8 540.5 9 630.5 10 549.0 11 457.5 12 412.5 13 442.0 14 486.0 15 522.0 16 568.0 17 600.0 18 652.5 19 726.5 20 753.5 21 789.5 22 890.5 23 1002.5 24 1145.0 25 1358.0 26 1688.5 27 2239.5 28 3090.0 29 4184.5 30 5603.0 31 7271.5 32 9441.5 33 11938.5 34 14376.0 35 17031.5 36 20396.0 37 23765.5 38 26967.0 39 30832.0 40 34685.0 41 38237.5 42 41981.0 43 47233.5 44 52475.0 45 57265.0 46 61497.0 47 65927.5 48 71824.0 49 76319.0 50 78554.5 51 79599.0 52 78298.0 53 76528.0 54 76282.0 55 74329.0 56 69849.0 57 64042.0 58 58994.5 59 53473.0 60 45605.0 61 37915.5 62 31616.0 63 24876.5 64 18516.0 65 14178.0 66 10963.5 67 8607.0 68 6887.5 69 5373.5 70 4159.0 71 3212.5 72 2428.5 73 1955.5 74 1527.5 75 1124.0 76 781.0 77 437.5 78 247.0 79 141.5 80 78.0 81 43.0 82 25.5 83 17.5 84 13.0 85 6.5 86 1.5 87 1.5 88 1.0 89 0.0 90 0.0 91 1.0 92 1.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026345885551078085 2 0.0016166793406343371 3 0.03179469369914196 4 0.1670568651988815 5 0.02436994413474723 6 0.07185241513930388 7 0.05508685160679963 8 0.04700345490362795 9 0.03395026615332108 10-11 0.06337981785412762 12-13 0.05852977983222461 14-15 0.060266213198091126 16-17 0.05218281649491944 18-19 0.03865061164368387 20-21 0.024968714260908097 22-23 0.003143543162344544 24-25 0.004341083414666275 26-27 0.0032333586812686743 28-29 0.004580591465130622 30-31 0.002275326479411289 32-33 0.00886179786718081 34-35 0.004550652958822578 36-37 0.0062571478183810455 38-39 0.002245387973103246 40-41 0.003323174200192804 42-43 0.00374231328850541 44-45 0.004460837439898449 46-47 0.004460837439898449 48-49 0.001796310378482597 50-51 0.0014669868090941207 52-53 9.879707081654281E-4 54-55 0.005149423084983444 56-57 8.083396703171686E-4 58-59 8.382781766252119E-4 60-61 8.981551892412984E-5 62-63 7.784011640091251E-4 64-65 0.0 66-67 0.0029339736181882414 68-69 5.38893113544779E-4 70-71 0.0 72-73 0.001796310378482597 74-75 0.004460837439898449 76-77 0.0016765563532504238 78-79 2.694465567723895E-4 80-81 0.0 82-83 0.00520930009759953 84-85 0.0016466178469423803 86-87 0.0 88-89 0.0011077247333976011 90-91 7.784011640091251E-4 92-93 0.0 94-95 0.003053727643420414 96-97 0.001796310378482597 98-99 5.688316198528223E-4 100-101 0.0 102-103 8.981551892412985E-4 104-105 0.0 106-107 7.185241513930387E-4 108-109 0.002275326479411289 110-111 8.981551892412984E-5 112-113 2.993850630804328E-4 114-115 0.0 116-117 0.0018861258974067266 118-119 0.0023052649857193326 120-121 0.003053727643420414 122-123 0.001407109796478034 124-125 0.10810794627834429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1670090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.15020559587441 #Duplication Level Percentage of deduplicated Percentage of total 1 80.50415498461952 50.03349783616241 2 10.786678642571914 13.407885906649442 3 3.460931553060367 6.452928227278522 4 1.6040554114143415 3.9876949442630503 5 0.9422509116725927 2.9280543941675865 6 0.5845667379607795 2.1798565769263254 7 0.4089839933566352 1.7792907490775631 8 0.29037805087032914 1.443764444969619 9 0.21780064821376 1.218271955885991 >10 1.130266079868204 12.172909972556408 >50 0.05129685381634483 2.1687169131651927 >100 0.017960334073951856 1.7875574478601874 >500 3.8617057216672624E-4 0.1614039035614789 >1k 2.8962792912504473E-4 0.2781667274762142 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 0.11741882174014573 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.987701261608656E-5 0.0 5 0.0 0.0 0.0 5.987701261608656E-5 0.0 6 0.0 0.0 0.0 5.987701261608656E-5 0.0 7 0.0 0.0 0.0 5.987701261608656E-5 5.987701261608656E-5 8 0.0 0.0 0.0 5.987701261608656E-5 5.987701261608656E-5 9 0.0 0.0 0.0 5.987701261608656E-5 1.7963103784825968E-4 10-11 0.0 0.0 0.0 5.987701261608656E-5 1.7963103784825968E-4 12-13 0.0 0.0 0.0 5.987701261608656E-5 1.7963103784825968E-4 14-15 0.0 0.0 0.0 5.987701261608656E-5 1.7963103784825968E-4 16-17 0.0 0.0 0.0 8.981551892412983E-5 1.7963103784825968E-4 18-19 0.0 0.0 0.0 1.1975402523217311E-4 1.7963103784825968E-4 20-21 0.0 0.0 0.0 1.496925315402164E-4 2.0956954415630297E-4 22-23 0.0 0.0 0.0 5.089546072367358E-4 2.694465567723895E-4 24-25 0.0 0.0 0.0 8.682166829332551E-4 2.993850630804328E-4 26-27 0.0 0.0 0.0 0.0013472327838619474 2.993850630804328E-4 28-29 0.0 0.0 0.0 0.004820099515594968 3.5926207569651937E-4 30-31 0.0 0.0 0.0 0.019370213581304002 3.892005820045626E-4 32-33 0.0 0.0 0.0 0.046344807764851 4.7901610092869246E-4 34-35 0.0 0.0 0.0 0.07966636528570317 4.7901610092869246E-4 36-37 0.0 0.0 0.0 0.12439449370991983 5.38893113544779E-4 38-39 0.0 0.0 0.0 0.189540683436222 5.38893113544779E-4 40-41 0.0 0.0 0.0 0.2666023986731254 5.38893113544779E-4 42-43 0.0 5.987701261608656E-5 0.0 0.3457298708452838 5.38893113544779E-4 44-45 0.0 5.987701261608656E-5 0.0 0.43716206911004796 5.688316198528223E-4 46-47 0.0 5.987701261608656E-5 0.0 0.5327257812453221 5.987701261608656E-4 48-49 0.0 5.987701261608656E-5 0.0 0.6317922986186373 5.987701261608656E-4 50-51 0.0 5.987701261608656E-5 0.0 0.7307690004730284 5.987701261608656E-4 52-53 0.0 5.987701261608656E-5 0.0 0.8339071547042375 6.885856450849954E-4 54-55 0.0 5.987701261608656E-5 0.0 0.9571639851744517 7.784011640091253E-4 56-57 0.0 5.987701261608656E-5 0.0 1.0880850732595249 8.382781766252118E-4 58-59 0.0 5.987701261608656E-5 0.0 1.2275985126550066 8.382781766252118E-4 60-61 0.0 5.987701261608656E-5 0.0 1.362740930129514 8.382781766252118E-4 62-63 0.0 5.987701261608656E-5 0.0 1.507972624229832 8.382781766252118E-4 64-65 0.0 5.987701261608656E-5 0.0 1.665059966828135 8.382781766252118E-4 66-67 0.0 5.987701261608656E-5 0.0 1.8485830104964402 8.682166829332551E-4 68-69 0.0 5.987701261608656E-5 0.0 2.047464507900772 8.981551892412984E-4 70-71 0.0 5.987701261608656E-5 0.0 2.243471908699531 8.981551892412984E-4 72-73 0.0 5.987701261608656E-5 0.0 2.434210132388075 9.879707081654281E-4 74-75 0.0 5.987701261608656E-5 0.0 2.645096970821932 0.0010179092144734715 76-77 0.0 5.987701261608656E-5 0.0 2.9001730445664604 0.0010179092144734715 78-79 0.0 5.987701261608656E-5 0.0 3.1755773640941505 0.001077786227089558 80-81 0.0 5.987701261608656E-5 0.0 3.432809010292859 0.0011077247333976014 82-83 0.0 5.987701261608656E-5 0.0 3.6907891191492674 0.0012574172649378178 84-85 0.0 5.987701261608656E-5 0.0 3.9721512014322577 0.0012574172649378178 86-87 0.0 5.987701261608656E-5 0.0 4.302133417959512 0.0012574172649378178 88-89 0.0 5.987701261608656E-5 0.0 4.674957637013574 0.0012574172649378178 90-91 0.0 5.987701261608656E-5 0.0 5.049937428521816 0.0012574172649378178 92-93 0.0 5.987701261608656E-5 0.0 5.406115838068607 0.0012574172649378178 94-95 0.0 5.987701261608656E-5 0.0 5.776784484668491 0.0013172942775539042 96-97 0.0 5.987701261608656E-5 0.0 6.206252357657371 0.0013172942775539042 98-99 0.0 5.987701261608656E-5 0.0 6.703351316396122 0.0013172942775539042 100-101 0.0 5.987701261608656E-5 0.0 7.214042356998725 0.0013172942775539042 102-103 0.0 5.987701261608656E-5 0.0 7.712278978977181 0.0013771712901699909 104-105 0.0 5.987701261608656E-5 0.0 8.220155799986827 0.001526863821710207 106-107 0.0 5.987701261608656E-5 0.0 8.784107443311438 0.0015568023280182505 108-109 0.0 5.987701261608656E-5 0.0 9.406139788873652 0.0015568023280182505 110-111 0.0 5.987701261608656E-5 0.0 10.071044075468986 0.0015568023280182505 112-113 0.0 5.987701261608656E-5 0.0 10.704842254010263 0.0015568023280182505 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2280 0.0 51.801613 1 GTATCAA 3805 0.0 48.127872 1 TCAACGC 4835 0.0 37.372726 4 GTACATG 8585 0.0 37.033936 1 CAACGCA 4985 0.0 36.090622 5 ATCAACG 5040 0.0 35.85476 3 TACATGG 8835 0.0 35.506992 2 TATCAAC 5115 0.0 35.329025 2 AACGCAG 5240 0.0 34.354904 6 ACATGGG 9305 0.0 33.26486 3 TATCACG 180 5.875336E-10 33.13311 2 CATGGGG 8250 0.0 31.589018 4 TATACCG 215 1.200533E-10 30.47943 5 ACGCAGA 6165 0.0 29.20028 7 ATGGGGG 4435 0.0 29.148666 5 CGCAGAG 6755 0.0 26.735683 8 GTGTACG 265 1.3405952E-9 24.760517 1 GCAGAGT 7675 0.0 23.064236 9 GTGGTAT 1475 0.0 22.646908 1 TGGGGGG 2920 0.0 22.25137 6 >>END_MODULE