FastQCFastQC Report
Mon 15 Aug 2016
SRR2921632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850477
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89550.038.550091
GTATCAA28900.038.3069951
TACATGG92100.036.9577372
ACATGGG94800.035.7796023
GGTATCA20450.035.2172161
CATGGGG79800.035.1226274
TCAACGC34350.030.83634
CAACGCA34850.030.2206785
ATGGGGG50900.029.45895
AACGCAG38050.028.9246856
TATCAAC38100.028.5821652
ATCAACG38550.027.6310653
GAGTACA63250.024.5608251
ACGCAGA45950.023.6883567
AGTACAT62500.023.5172162
CGCAGAG49500.021.9900868
TGGGGGG36900.021.2813456
ATGGGGA31700.021.21065
GTGTACG3652.579327E-919.5682221
TATACCG1856.9556956E-419.2980425
Produced by FastQC (version 0.11.5)