##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921632_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850477
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.998603062885948	31.0	31.0	33.0	27.0	34.0
2	30.164361405194445	31.0	31.0	34.0	27.0	34.0
3	30.27389316376264	31.0	31.0	34.0	27.0	34.0
4	33.86319257142888	37.0	35.0	37.0	30.0	37.0
5	33.88343491975312	37.0	35.0	37.0	30.0	37.0
6	34.15398354046011	37.0	35.0	37.0	30.0	37.0
7	34.16212576541076	37.0	35.0	37.0	30.0	37.0
8	34.137556424640785	37.0	35.0	37.0	30.0	37.0
9	35.83604713811628	39.0	37.0	39.0	31.0	39.0
10-11	35.85985856619672	39.0	37.0	39.0	31.0	39.0
12-13	35.80539639238964	39.0	37.0	39.0	31.0	39.0
14-15	37.13137315405703	41.0	38.0	41.0	31.0	41.0
16-17	37.08612725259487	41.0	38.0	41.0	31.0	41.0
18-19	37.041705192769214	41.0	38.0	41.0	31.0	41.0
20-21	37.09811740432332	41.0	38.0	41.0	31.0	41.0
22-23	37.023766574780446	40.0	38.0	41.0	30.0	41.0
24-25	37.01112118659135	40.5	38.0	41.0	30.0	41.0
26-27	36.96711118268425	40.0	38.0	41.0	30.0	41.0
28-29	36.91744479936795	40.0	38.0	41.0	30.0	41.0
30-31	36.82775981544218	40.0	38.0	41.0	30.0	41.0
32-33	36.73836989057416	40.0	38.0	41.0	30.0	41.0
34-35	36.61497197749553	40.0	38.0	41.0	30.0	41.0
36-37	36.51403124707846	40.0	37.5	41.0	30.0	41.0
38-39	36.39897226498897	40.0	37.0	41.0	29.0	41.0
40-41	36.293033093629376	40.0	37.0	41.0	28.5	41.0
42-43	36.05183420274881	40.0	36.5	41.0	27.5	41.0
44-45	35.71883789963344	40.0	36.0	41.0	27.0	41.0
46-47	35.48116890942173	39.5	35.0	41.0	26.5	41.0
48-49	35.28633401009577	39.0	35.0	41.0	26.0	41.0
50-51	35.07979699288346	39.0	35.0	41.0	26.0	41.0
52-53	34.87613193787331	39.0	35.0	40.5	25.0	41.0
54-55	34.62420338107418	38.5	34.5	40.0	24.0	41.0
56-57	34.37074521866524	38.0	34.0	40.0	23.5	41.0
58-59	34.363482226474574	38.0	34.0	40.0	24.0	41.0
60-61	34.13930678414268	37.5	34.0	40.0	24.0	41.0
62-63	33.82546419112478	37.0	34.0	40.0	23.0	41.0
64-65	33.51634551523742	36.5	34.0	39.5	22.5	41.0
66-67	33.18657675831691	36.0	33.5	39.0	22.0	41.0
68-69	32.844849463138424	35.5	33.0	39.0	22.0	40.5
70-71	32.47759388525229	35.0	33.0	37.5	20.5	40.0
72-73	32.13005511551886	35.0	33.0	37.0	20.0	39.0
74-75	31.763346423651846	35.0	33.0	37.0	19.0	39.0
76-77	31.4694703041432	35.0	32.0	36.0	18.0	38.5
78-79	31.199598535945057	35.0	32.0	36.0	17.5	37.0
80-81	30.94291904195513	35.0	32.0	35.0	16.0	37.0
82-83	30.727842875107335	35.0	32.0	35.0	14.0	36.5
84-85	30.52008536177429	35.0	32.0	35.0	10.5	36.0
86-87	30.353388342573293	35.0	32.0	35.0	8.5	36.0
88-89	30.188646224730164	35.0	31.0	35.0	7.0	35.5
90-91	30.00419702595601	35.0	31.0	35.0	7.0	35.0
92-93	29.931695449335496	34.0	31.0	35.0	5.5	35.0
94-95	29.80508404049334	34.0	31.0	35.0	2.0	35.0
96-97	29.711436834934993	34.0	31.0	35.0	2.0	35.0
98-99	29.611218350727945	34.0	31.0	35.0	2.0	35.0
100-101	29.49972709739165	34.0	31.0	35.0	2.0	35.0
102-103	28.610141060926452	33.5	29.0	34.5	2.0	35.0
104-105	29.54623078265766	34.0	31.0	35.0	2.0	35.0
106-107	29.824193978093213	35.0	32.0	35.0	2.0	35.0
108-109	29.818159317840752	35.0	32.0	35.0	2.0	35.0
110-111	29.749093612079477	35.0	32.0	35.0	2.0	35.0
112-113	29.65652558772684	35.0	31.5	35.0	2.0	35.0
114-115	29.539068575291665	35.0	31.0	35.0	2.0	35.0
116-117	29.41298405762406	35.0	31.0	35.0	2.0	35.0
118-119	29.118699935205896	35.0	31.0	35.0	2.0	35.0
120-121	28.99057810499671	35.0	31.0	35.0	2.0	35.0
122-123	28.732283892207253	34.5	31.0	35.0	2.0	35.0
124-125	27.953248810982252	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62997.0
3	10657.0
4	5652.0
5	4908.0
6	5358.0
7	5944.0
8	6027.0
9	6528.0
10	6813.0
11	7035.0
12	6929.0
13	6944.0
14	6991.0
15	6333.0
16	5714.0
17	5321.0
18	4974.0
19	4847.0
20	4797.0
21	5033.0
22	5860.0
23	7712.0
24	10644.0
25	13521.0
26	15258.0
27	17549.0
28	20937.0
29	25172.0
30	30658.0
31	38687.0
32	50861.0
33	70630.0
34	110587.0
35	186052.0
36	380703.0
37	556643.0
38	129142.0
39	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.119510283505846	16.934731493743413	11.406556579551903	25.539201643198833
2	17.574208597458455	19.194224043677455	37.274136879009845	25.95743047985425
3	20.67425239796878	22.996720560163777	27.965439416834748	28.3635876250327
4	13.961084934977618	15.223430323744546	34.08743296982196	36.728051771455874
5	15.505714050987581	36.85427764709952	32.44096777820056	15.19904052371234
6	35.00103302767973	33.1558076167132	16.929647029044844	14.913512326562225
7	30.686079858813148	30.979354018245285	19.365178235423887	18.96938788751768
8	28.253433267211754	33.598792835080445	18.856220970435597	19.291552927272207
9	28.177202928445094	15.460229017206562	18.04132125976761	38.32124679458073
10-11	27.08207743590936	24.799403332707975	26.224246556160306	21.89427267522236
12-13	26.808593204248442	22.234241752171823	26.411399234527394	24.545765809052337
14-15	24.449052323238494	23.680094531186022	23.491659195830856	28.37919394974463
16-17	25.095615395551768	26.602865407579714	24.124649500313268	24.17686969655525
18-19	25.07617237337762	26.029894818332405	25.260007828076414	23.633924980213564
20-21	25.67633384272005	25.02914964131211	25.144261669451446	24.150254846516393
22-23	25.808919167008142	24.527411945200978	25.014271705155224	24.64939718263566
24-25	25.22734392294051	24.923786235492763	24.988898021536894	24.859971820029834
26-27	24.878195674081248	25.21811786636449	25.167433642863013	24.73625281669125
28-29	25.001189389778165	25.48889326199878	25.02389592190683	24.48602142631622
30-31	25.43358303326909	25.106938004200497	24.988155536654862	24.471323425875553
32-33	25.055616112469547	25.165618580092474	24.982992861731763	24.795772445706216
34-35	25.098110239200306	25.385777732124854	25.029585798816566	24.486526229858274
36-37	25.20750733936624	25.227774366912104	25.056639586314837	24.508078707406817
38-39	25.140634252031173	25.463968038493046	24.98391303607561	24.411484673400174
40-41	25.189568207251867	25.196352981245912	25.095446362442086	24.51863244906013
42-43	25.038356874468093	25.149569513845364	24.975737266828446	24.836336344858097
44-45	24.807381836086876	25.17294422167128	25.228560943008805	24.79111299923304
46-47	24.788275534770488	25.11278097949997	25.248210044507	24.85073344122254
48-49	24.747341518156382	25.39001517896913	25.18689916580537	24.67574413706912
50-51	24.727153903037067	25.405633900269414	25.203519674109053	24.66369252258447
52-53	24.918027647358347	25.259457495997328	25.08897229966908	24.733542556975245
54-55	24.717764384746356	25.044971757520067	25.270695386611173	24.966568471122404
56-57	24.75271929051693	25.26430752482537	25.032742836170147	24.95023034848756
58-59	24.71198777821228	25.30632166186477	25.22481425039261	24.75687630953034
60-61	24.982689572825684	25.224657183273237	25.297547459759002	24.495105784142083
62-63	24.827205971778913	25.337115243765655	25.198280032539927	24.637398751915505
64-65	24.72106430682548	25.104077255538716	25.183885132870792	24.990973304765014
66-67	24.75314264401026	25.209667262176648	25.075123448948762	24.962066644864333
68-69	24.55244031208709	25.211770101572355	25.244282551840875	24.991507034499683
70-71	24.45357702417626	25.056226367354974	25.48070840897762	25.00948819949115
72-73	24.583690977845137	25.316069463392697	25.249261108240344	24.85097845052182
74-75	24.52819787504614	25.289507263420667	25.24029900032373	24.94199586120946
76-77	24.538054714039017	24.997743444503598	25.248369739996164	25.21583210146122
78-79	24.42744935030116	25.110147993714634	25.44874057505604	25.013662080928167
80-81	24.54155533509194	25.25814917399561	25.26785113462943	24.93244435628302
82-83	24.602670108759987	25.19879332143087	25.348141685814934	24.850394883994213
84-85	24.587505435115997	25.259095256201032	25.120058976244387	25.03334033243858
86-87	24.397439850734532	25.357455287516828	25.282325289649116	24.96277957209953
88-89	24.32131784118967	25.116401881888983	25.37285357848627	25.189426698435074
90-91	24.53613892238614	24.949664039557444	25.407457775899168	25.10673926215725
92-93	24.540874976287796	25.047356852597368	25.452018118015708	24.959750053099125
94-95	24.554068343534176	24.89902070281645	25.45648425312072	25.090426700528656
96-97	24.434722356592296	24.86389287194823	25.483043157025982	25.21834161443349
98-99	24.282824843642967	24.94246304380429	25.41418907142365	25.360523041129095
100-101	24.222721968835916	25.0195241670004	25.550824717027936	25.206929147135742
102-103	24.043628581503654	24.937492991351835	25.73267563503419	25.286202792110323
104-105	24.065144474417274	25.065452789549386	25.54357092693688	25.32583180909646
106-107	24.0825234428703	24.94998530092042	25.626818470690466	25.340672785518812
108-109	24.062388273391345	25.00030401798273	25.622797727512868	25.314509981113055
110-111	23.91975337830809	25.253864542349397	25.64774266521224	25.17863941413027
112-113	23.80267468067497	25.295465666969207	25.66724482166679	25.234614830689033
114-115	23.831773301636364	25.310806037845573	25.660897742685435	25.196522917832624
116-117	23.70286622835521	25.206953139080884	25.700506145160517	25.389674487403386
118-119	23.714174473608846	25.14122127787205	25.71428881075549	25.430315437763607
120-121	23.660046372591694	25.260579000797918	25.830846771306277	25.248527855304108
122-123	23.54017537242051	25.337348186560977	25.744976119477194	25.377500321541323
124-125	23.482312471050204	25.253491118358557	25.805071963534033	25.459124447057206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	371.0
1	296.5
2	208.0
3	162.0
4	128.0
5	139.0
6	160.5
7	171.5
8	196.5
9	207.0
10	213.0
11	233.5
12	251.0
13	281.0
14	302.0
15	324.5
16	355.0
17	382.0
18	438.5
19	486.0
20	531.5
21	605.0
22	702.0
23	835.0
24	979.5
25	1231.5
26	1639.5
27	2220.5
28	2999.5
29	4137.0
30	5806.5
31	7730.5
32	10327.5
33	13161.5
34	15923.5
35	18922.0
36	22689.5
37	27007.5
38	30812.0
39	35051.5
40	39320.0
41	42864.5
42	47004.5
43	52558.5
44	58372.0
45	63341.0
46	68093.5
47	73510.0
48	78908.5
49	82958.5
50	85411.5
51	86920.0
52	86173.5
53	84959.0
54	84766.0
55	82515.5
56	77672.5
57	71311.5
58	65170.0
59	58592.0
60	50518.0
61	42808.0
62	35860.0
63	28190.0
64	21193.5
65	16279.0
66	12608.5
67	9857.0
68	7681.0
69	5988.0
70	4825.0
71	3806.0
72	2932.0
73	2340.0
74	1801.5
75	1424.5
76	1054.0
77	705.5
78	468.0
79	301.5
80	214.0
81	154.0
82	117.5
83	83.0
84	68.0
85	54.0
86	43.0
87	40.0
88	36.5
89	28.0
90	21.0
91	18.0
92	16.5
93	18.0
94	19.0
95	12.0
96	10.0
97	8.0
98	7.0
99	7.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023075131439083005
2	0.010051462406719997
3	0.008700459395064083
4	0.01032166300905118
5	0.0162660762603372
6	0.08338390587940298
7	0.05474264203229761
8	0.04615026287816601
9	0.026209458426124724
10-11	0.04701490480562579
12-13	0.013374929815393545
14-15	0.02731728089568257
16-17	0.03339679444813418
18-19	0.039179087338021494
20-21	0.029181665051767735
22-23	0.03582859986911483
24-25	0.03142433005111655
26-27	0.042637655047860636
28-29	0.042637655047860636
30-31	0.024669314992836984
32-33	0.028019802461743647
34-35	0.041718972999934614
36-37	0.009781261804388814
38-39	0.020346105355538057
40-41	0.0403679699882787
42-43	0.022561750294653755
44-45	0.016968597826398275
46-47	0.02329129192094795
48-49	0.029668026135963865
50-51	0.02880338420850408
52-53	0.02502057577586752
54-55	0.023075131439083005
56-57	0.02453421469167139
58-59	0.01829258077782107
60-61	0.02356149252327913
62-63	0.02388573324607655
64-65	0.022102409270690748
66-67	0.028208942883375478
68-69	0.02250771017418752
70-71	0.04382653769811784
72-73	0.022102409270690748
74-75	0.009619141442990104
76-77	0.016860517585465803
78-79	0.025182696137266227
80-81	0.018130460416422363
82-83	0.02377765300514408
84-85	0.012996648972129888
86-87	0.01894106222341591
88-89	0.022750890716285582
90-91	0.02204836915022451
92-93	0.008862579756462794
94-95	0.011969886683271395
96-97	0.015131233730546234
98-99	0.00780879740737118
100-101	0.011132264816044728
102-103	0.004566390179396988
104-105	0.005268911745458063
106-107	0.0011348425297909674
108-109	0.01391533102005591
110-111	0.001702263794686451
112-113	0.0027290260835449455
114-115	0.013374929815393545
116-117	0.00640375427524903
118-119	0.2733079092579913
120-121	0.0016482236742202145
122-123	8.106018069935481E-4
124-125	0.30740722527218656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1850477.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.4557776877108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24686509186112	51.55583009092572
2	10.175625099033388	12.914044082355058
3	3.3746332939421246	6.42419940233816
4	1.5161000105388462	3.8482122088435613
5	0.9575227166034304	3.038017431786009
6	0.6064778705860695	2.3090714957055525
7	0.4153431290540427	1.8449144882960056
8	0.30783739925505743	1.5627249256873599
9	0.21124735756559646	1.2064378822918955
>10	1.1359471792009683	12.459967753909838
>50	0.04252241566850572	1.793362482820781
>100	0.009623076658054836	0.9375672220912035
>500	2.5536003278429546E-4	0.1056505329488778
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.4040120466236546E-5
7	0.0	0.0	0.0	0.0	5.4040120466236546E-5
8	0.0	0.0	0.0	0.0	1.0808024093247309E-4
9	0.0	0.0	0.0	0.0	1.6212036139870962E-4
10-11	0.0	0.0	0.0	0.0	2.1616048186494618E-4
12-13	0.0	0.0	0.0	0.0	2.702006023311827E-4
14-15	0.0	0.0	0.0	0.0	4.5934102396301066E-4
16-17	0.0	0.0	0.0	0.0	5.94441325128602E-4
18-19	0.0	0.0	0.0	0.0	7.025215660610751E-4
20-21	0.0	0.0	0.0	1.6212036139870965E-4	7.565616865273116E-4
22-23	0.0	0.0	0.0	3.782808432636558E-4	7.835817467604299E-4
24-25	0.0	0.0	0.0	8.376218672266663E-4	8.106018069935481E-4
26-27	0.0	0.0	0.0	0.0012969628911896772	8.106018069935481E-4
28-29	0.0	0.0	0.0	0.0036206880712378485	8.106018069935481E-4
30-31	0.0	0.0	0.0	0.013969371140522146	8.376218672266664E-4
32-33	0.0	0.0	0.0	0.035639459447483	8.646419274597847E-4
34-35	0.0	0.0	0.0	0.06463198407761891	9.186820479260212E-4
36-37	0.0	0.0	0.0	0.10618883671615481	9.186820479260212E-4
38-39	0.0	0.0	0.0	0.17414428820244726	9.727221683922578E-4
40-41	0.0	0.0	0.0	0.2537724057094468	9.727221683922578E-4
42-43	0.0	0.0	0.0	0.33126593845803	9.727221683922578E-4
44-45	0.0	0.0	0.0	0.4167574090356162	9.99742228625376E-4
46-47	0.0	0.0	0.0	0.5111114593696653	0.0011078224695578493
48-49	0.0	0.0	0.0	0.6055465698844136	0.0011348425297909674
50-51	0.0	0.0	0.0	0.7048452912411233	0.0011618625900240856
52-53	0.0	0.0	0.0	0.8108449875356463	0.0012699428309565588
54-55	0.0	0.0	0.0	0.9327054591870096	0.0013510030116559136
56-57	0.0	0.0	0.0	1.0642391124018293	0.0013510030116559136
58-59	0.0	5.4040120466236546E-5	0.0	1.1989611327241572	0.0013510030116559136
60-61	0.0	5.4040120466236546E-5	0.0	1.3336020928657855	0.0013510030116559136
62-63	0.0	5.4040120466236546E-5	0.0	1.4852656909542783	0.0014590832525883867
64-65	0.0	5.4040120466236546E-5	0.0	1.6363078276574092	0.0014590832525883867
66-67	0.0	5.4040120466236546E-5	0.0	1.811911199112445	0.0014590832525883867
68-69	0.0	5.4040120466236546E-5	0.0	2.0013758614670705	0.0014590832525883867
70-71	0.0	5.4040120466236546E-5	0.0	2.203918232974525	0.0015131233730546231
72-73	0.0	5.4040120466236546E-5	0.0	2.393626075871248	0.0017833239753858058
74-75	0.0	5.4040120466236546E-5	0.0	2.5928720000302627	0.0018373640958520424
76-77	0.0	5.4040120466236546E-5	0.0	2.8288652061063173	0.0018373640958520424
78-79	0.0	5.4040120466236546E-5	0.0	3.090473429283368	0.001891404216318279
80-81	0.0	5.4040120466236546E-5	0.0	3.3415438289695034	0.0019454443367845156
82-83	0.0	5.4040120466236546E-5	0.0	3.60077428684604	0.001999484457250752
84-85	0.0	5.4040120466236546E-5	0.0	3.878324345560631	0.001999484457250752
86-87	0.0	8.106018069935483E-5	0.0	4.193837588902753	0.001999484457250752
88-89	0.0	1.0808024093247309E-4	0.0	4.5406941021152925	0.001999484457250752
90-91	0.0	1.0808024093247309E-4	0.0	4.896575315445693	0.001999484457250752
92-93	0.0	1.0808024093247309E-4	0.0	5.2514027464270026	0.0020535245777169885
94-95	0.0	1.0808024093247309E-4	0.0	5.625495480354525	0.002215644939115698
96-97	0.0	1.0808024093247309E-4	0.0	6.042442029811773	0.0022426649993488165
98-99	0.0	1.0808024093247309E-4	0.0	6.507592366724904	0.0022967051198150533
100-101	0.0	1.0808024093247309E-4	0.0	6.993953450921033	0.0023237251800481713
102-103	0.0	1.0808024093247309E-4	0.0	7.485880667525183	0.002377765300514408
104-105	0.0	1.0808024093247309E-4	0.0	7.9868866243676635	0.002404785360747526
106-107	0.0	1.0808024093247309E-4	0.0	8.526477227223035	0.0024318054209806444
108-109	0.0	1.0808024093247309E-4	0.0	9.122782936507722	0.002485845541446881
110-111	0.0	1.0808024093247309E-4	0.0	9.747675869519048	0.002593925782379354
112-113	0.0	1.0808024093247309E-4	0.0	10.361220377232465	0.002593925782379354
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8955	0.0	38.55009	1
GTATCAA	2890	0.0	38.306995	1
TACATGG	9210	0.0	36.957737	2
ACATGGG	9480	0.0	35.779602	3
GGTATCA	2045	0.0	35.217216	1
CATGGGG	7980	0.0	35.122627	4
TCAACGC	3435	0.0	30.8363	4
CAACGCA	3485	0.0	30.220678	5
ATGGGGG	5090	0.0	29.4589	5
AACGCAG	3805	0.0	28.924685	6
TATCAAC	3810	0.0	28.582165	2
ATCAACG	3855	0.0	27.631065	3
GAGTACA	6325	0.0	24.560825	1
ACGCAGA	4595	0.0	23.688356	7
AGTACAT	6250	0.0	23.517216	2
CGCAGAG	4950	0.0	21.990086	8
TGGGGGG	3690	0.0	21.281345	6
ATGGGGA	3170	0.0	21.2106	5
GTGTACG	365	2.579327E-9	19.568222	1
TATACCG	185	6.9556956E-4	19.298042	5
>>END_MODULE