FastQCFastQC Report
Mon 15 Aug 2016
SRR2921632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850477
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.10667519780035095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.052.4489331
GTATCAA41100.049.49691
TCAACGC51950.038.5683364
CAACGCA53300.037.656425
TATCAAC55150.036.551672
ATCAACG55300.036.343693
GTACATG91100.036.2136421
AACGCAG56800.035.3596
TACATGG94850.034.5828932
ACATGGG97500.033.4585383
CATGGGG84000.032.7974364
ATGGGGG49300.030.4431745
ACGCAGA68950.029.126657
CGCAGAG73600.027.284978
GTATAGG4550.024.9119451
GCAGAGT81950.024.5775819
ATGGGGA29950.024.0616195
TGGGGGG33100.023.4063556
AGAGTAC74400.022.7065710-11
AGTACAT67100.020.7983722
Produced by FastQC (version 0.11.5)