##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921632_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1850477 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807795503537736 33.0 31.0 34.0 30.0 34.0 2 31.976382305751436 34.0 31.0 34.0 30.0 34.0 3 32.05311765560988 34.0 31.0 34.0 30.0 34.0 4 35.46336323012931 37.0 35.0 37.0 35.0 37.0 5 35.328106753015575 37.0 35.0 37.0 33.0 37.0 6 35.360471921564006 37.0 35.0 37.0 33.0 37.0 7 35.245690165292515 37.0 35.0 37.0 33.0 37.0 8 35.27397422394334 37.0 36.0 37.0 33.0 37.0 9 36.9974849727935 39.0 38.0 39.0 34.0 39.0 10-11 36.95353063021048 39.0 38.0 39.0 34.0 39.0 12-13 36.8836405424115 39.0 38.0 39.0 34.0 39.0 14-15 38.32478085380148 41.0 39.0 41.0 34.0 41.0 16-17 38.33753918584236 41.0 39.0 41.0 34.0 41.0 18-19 38.33720305629305 41.0 39.0 41.0 34.0 41.0 20-21 38.26941702058443 41.0 39.0 41.0 34.0 41.0 22-23 38.27325657114355 41.0 39.0 41.0 34.0 41.0 24-25 38.235252316024464 41.0 39.0 41.0 34.0 41.0 26-27 38.17099374917927 41.0 39.0 41.0 34.0 41.0 28-29 38.10292805584722 41.0 39.0 41.0 34.0 41.0 30-31 37.99848498522273 41.0 38.0 41.0 33.0 41.0 32-33 37.89262065942998 41.0 38.0 41.0 33.0 41.0 34-35 37.761312353517496 41.0 38.0 41.0 33.0 41.0 36-37 37.687651886513585 40.5 38.0 41.0 33.0 41.0 38-39 37.60882572439431 40.0 38.0 41.0 33.0 41.0 40-41 37.49082858095507 40.0 38.0 41.0 32.5 41.0 42-43 37.331921445119285 40.0 38.0 41.0 32.0 41.0 44-45 37.186624854024124 40.0 37.0 41.0 32.0 41.0 46-47 37.00630215884877 40.0 37.0 41.0 31.0 41.0 48-49 36.82781871917349 40.0 37.0 41.0 31.0 41.0 50-51 36.57754054765339 40.0 36.0 41.0 30.0 41.0 52-53 36.4481895749042 40.0 36.0 41.0 30.0 41.0 54-55 36.31455349080264 40.0 35.0 41.0 30.0 41.0 56-57 36.110176457205355 39.0 35.0 41.0 30.0 41.0 58-59 35.910869467710214 39.0 35.0 41.0 30.0 41.0 60-61 35.647785679043835 38.5 35.0 41.0 30.0 41.0 62-63 35.28975042651165 37.5 35.0 40.5 29.0 41.0 64-65 34.99240060805944 37.0 35.0 40.0 29.0 41.0 66-67 34.68366426602438 36.5 35.0 39.5 29.0 41.0 68-69 34.31119570791747 36.0 35.0 39.0 28.5 41.0 70-71 33.93694895964662 35.5 35.0 39.0 28.0 40.5 72-73 33.61592200281333 35.0 34.0 37.0 28.0 39.5 74-75 33.29991456256954 35.0 34.0 37.0 28.0 39.0 76-77 32.98288495344714 35.0 34.0 36.5 27.0 39.0 78-79 32.72759888396343 35.0 34.0 36.0 27.0 38.0 80-81 32.482255656244305 35.0 34.0 36.0 26.5 37.0 82-83 32.2717442583723 35.0 34.0 35.0 26.0 37.0 84-85 32.06749421905811 35.0 34.0 35.0 26.0 36.0 86-87 31.949631905719443 35.0 34.0 35.0 26.0 36.0 88-89 31.86196721169731 35.0 34.0 35.0 26.0 36.0 90-91 31.759443916352378 35.0 34.0 35.0 25.0 35.5 92-93 31.685360585405817 35.0 33.0 35.0 25.0 35.0 94-95 31.620435974075875 35.0 33.0 35.0 25.0 35.0 96-97 31.558476814356517 35.0 33.0 35.0 25.0 35.0 98-99 31.48501089178628 35.0 33.0 35.0 25.0 35.0 100-101 31.418795261978396 35.0 33.0 35.0 24.0 35.0 102-103 30.703416470456 34.0 31.5 35.0 23.5 35.0 104-105 31.243731210925617 35.0 33.0 35.0 24.0 35.0 106-107 31.34577165779418 35.0 33.0 35.0 24.0 35.0 108-109 31.33103761894906 35.0 33.0 35.0 24.0 35.0 110-111 31.26046311302437 35.0 33.0 35.0 24.0 35.0 112-113 31.216280721132982 35.0 33.0 35.0 24.0 35.0 114-115 31.088407745678545 35.0 33.0 35.0 23.0 35.0 116-117 31.27168049103015 35.0 33.0 35.0 24.0 35.0 118-119 31.252053119276813 35.0 34.0 35.0 23.5 35.0 120-121 31.16972380634831 35.0 33.5 35.0 23.0 35.0 122-123 31.03093418615849 35.0 33.0 35.0 19.5 35.0 124-125 30.372455048076795 34.5 32.0 35.0 9.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26610.0 3 7203.0 4 3992.0 5 3225.0 6 3313.0 7 3631.0 8 4229.0 9 4457.0 10 4862.0 11 5220.0 12 5662.0 13 6835.0 14 8399.0 15 7875.0 16 5043.0 17 4412.0 18 4087.0 19 3768.0 20 3776.0 21 3788.0 22 4128.0 23 5379.0 24 6923.0 25 8911.0 26 11012.0 27 12933.0 28 15810.0 29 19174.0 30 23271.0 31 29661.0 32 38334.0 33 52925.0 34 82298.0 35 145917.0 36 344288.0 37 696076.0 38 232778.0 39 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32043917576706 17.345518486106297 10.754260380073417 27.579781958053225 2 16.327990825975633 19.576457948965707 36.28533074800507 27.810220477053587 3 19.00345037657917 23.0704405487088 28.339413700854564 29.586695373857463 4 13.616941350700639 14.802192853307467 33.8250004330279 37.755865362963995 5 14.92000499445151 36.91618564441127 32.59571482546716 15.56809453567006 6 35.107925262087214 33.35141235361266 16.473736663117556 15.066925721182573 7 30.87909539028163 31.099743775374428 19.1446671593994 18.87649367494454 8 28.83813591393199 33.56257771530519 18.52916689192842 19.070119478834403 9 28.13194642656357 14.88135395313838 18.115631784253182 38.87106783604487 10-11 27.291632421770238 24.56994718355553 26.11569872565729 22.02272166901695 12-13 27.074281258988993 21.88085974250115 26.616721204400797 24.42813779410906 14-15 24.51091524441329 23.217809349064787 23.856873788355287 28.414401618166636 16-17 24.8338038799279 26.30364474471 24.473432950083854 24.389118425278248 18-19 24.777128289016673 25.583174895436393 26.0418654460867 23.597831369460238 20-21 25.45378872335394 24.62403297185906 25.91820546603155 24.00397283875545 22-23 25.30489139451571 24.187654323656393 25.777218383139335 24.730235898688562 24-25 24.7893881400197 24.529766142946123 25.801827459998133 24.879018257036044 26-27 24.524626008863436 24.858930027185842 25.970439548176177 24.646004415774545 28-29 24.63935256804318 25.096581121521844 25.849207260641354 24.414859049793623 30-31 25.09259321807199 24.766074958151677 25.736010365144363 24.405321458631967 32-33 24.80369473822452 24.762512383080214 25.731567788480508 24.702225090214757 34-35 24.842188034095653 24.89663623602147 25.836874267887854 24.42430146199502 36-37 24.9082542653221 24.78760197010471 25.833831660189094 24.470312104384096 38-39 24.815732317787244 25.012010702359934 25.8220373285604 24.350219651292427 40-41 24.91030361395778 24.711671852006774 25.881872602219293 24.496151931816154 42-43 24.793200621809987 24.738780088353653 25.6397182345806 24.828301055255757 44-45 24.605170111849667 24.840202217321742 25.74852645570342 24.806101215125175 46-47 24.690753545883695 24.749471056023474 25.638502257638272 24.921273140454552 48-49 24.653581829589445 25.0875603978952 25.54518202997454 24.713675742540815 50-51 24.633635521824342 25.196716142139824 25.539552502356866 24.630095833678972 52-53 24.80494688475688 24.97355383736184 25.402285650980904 24.819213626900375 54-55 24.577315409038707 24.822184692292105 25.621455110450587 24.979044788218605 56-57 24.57214005494311 25.04110465535722 25.431521197843022 24.955234091856642 58-59 24.587305095654646 25.021116382475917 25.54971496261198 24.84186355925746 60-61 24.820080028079268 24.99171158980489 25.668753787877762 24.519454594238073 62-63 24.708943913660363 25.08347251047645 25.574242505712768 24.633341070150415 64-65 24.548454263414243 24.816223060324447 25.552006320532488 25.08331635572882 66-67 24.492126892753134 25.026250896421505 25.48350016996206 24.99812204086331 68-69 24.410486012975117 25.028222635932053 25.53247426998079 25.02881708111204 70-71 24.368635762562842 24.83764996808931 25.69842802693576 25.09528624241209 72-73 24.588454988028495 25.08898580901631 25.37321619694391 24.949343006011286 74-75 24.415261842232905 25.13406033735242 25.416705059691807 25.033972760722868 76-77 24.385998379847422 24.801817726890263 25.40134983893046 25.410834054331854 78-79 24.23893127894023 24.89022404265719 25.61290394053198 25.257940737870605 80-81 24.387441724485093 25.137302436074588 25.34635664209823 25.128899197342093 82-83 24.427197499319057 25.03326350968668 25.399135094705077 25.140403896289186 84-85 24.349088299396414 25.055000343161073 25.232147112880536 25.363764244561978 86-87 24.137371067027583 25.15662177914127 25.420175446655108 25.285831707176044 88-89 24.03767067396288 24.92774761707642 25.486285017006598 25.548296691954096 90-91 24.172921421117678 24.845349180932946 25.53498888654112 25.44674051140825 92-93 24.208352765259985 24.92865353095445 25.533335458911406 25.32965824487416 94-95 24.13255293203067 24.746631229580597 25.517701294655172 25.603114543733557 96-97 23.927719125314518 24.694425349969304 25.649248182061857 25.72860734265432 98-99 23.89093327158304 24.80789272525505 25.455054909215992 25.846119093945923 100-101 23.83312518880267 24.832651256946182 25.605100738890567 25.72912281536058 102-103 23.73068713015753 24.6973709097787 25.73095733470237 25.840984625361397 104-105 23.74395899003338 24.869290458622288 25.550141936376402 25.836608614967925 106-107 23.815369201342044 24.68361253860863 25.590521926839294 25.91049633321003 108-109 23.699903184639027 24.70004936691725 25.61243214075435 25.987615307689378 110-111 23.620787300132505 25.01215903367566 25.531890173123024 25.83516349306881 112-113 23.54108286338605 24.999331252244357 25.55356777603138 25.90601810833822 114-115 23.4227715340423 25.08304615512649 25.561247181132217 25.932935129698993 116-117 23.335314844037093 24.911993904152528 25.607287996368434 26.145403255441952 118-119 23.378699414405748 24.837686200165585 25.548224518868068 26.2353898665606 120-121 23.293441488959264 24.93158310005296 25.58021962581468 26.194755785173097 122-123 23.14974135937105 25.062930842543736 25.454593600238645 26.33273419784657 124-125 22.952673466881812 25.015700502037415 25.504836728302067 26.526789302778703 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 28.0 1 23.5 2 27.5 3 56.5 4 63.5 5 61.5 6 138.5 7 345.0 8 567.5 9 626.0 10 540.5 11 456.0 12 454.5 13 500.5 14 533.0 15 582.0 16 622.0 17 622.0 18 667.5 19 726.5 20 774.5 21 852.5 22 947.0 23 1043.5 24 1174.5 25 1387.5 26 1764.5 27 2370.5 28 3188.0 29 4267.0 30 5792.0 31 7612.5 32 10151.0 33 12868.5 34 15398.0 35 18426.5 36 22105.5 37 26216.5 38 30113.0 39 34370.0 40 38912.5 41 42783.0 42 46518.0 43 51762.5 44 57560.0 45 62702.5 46 67981.5 47 73207.0 48 78550.5 49 83012.5 50 85598.0 51 87428.5 52 87362.0 53 86318.5 54 85911.5 55 83291.5 56 78645.5 57 72544.0 58 66045.5 59 59426.5 60 51380.5 61 43596.5 62 35936.0 63 27822.5 64 20703.5 65 15577.0 66 11996.0 67 9404.0 68 7354.0 69 5614.5 70 4315.5 71 3284.5 72 2477.5 73 2045.5 74 1614.0 75 1222.0 76 883.5 77 518.0 78 300.5 79 170.0 80 94.0 81 58.0 82 40.5 83 20.0 84 10.5 85 7.0 86 4.5 87 3.0 88 2.5 89 1.0 90 1.5 91 2.0 92 2.0 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025182696137266227 2 0.0018914042163182792 3 0.028857424328970316 4 0.1632552039285006 5 0.022913011077684294 6 0.06419966311388901 7 0.05014923179266751 8 0.0419891736022658 9 0.032694272882073105 10-11 0.056904246850947075 12-13 0.05366183962297289 14-15 0.05277017763527998 16-17 0.04620430299863224 18-19 0.03490991782118881 20-21 0.022399629933255047 22-23 0.003458567709839139 24-25 0.005106791384059353 26-27 0.0035396278905384937 28-29 0.00483659078172817 30-31 0.002404785360747526 32-33 0.009186820479260213 34-35 0.005106791384059353 36-37 0.00640375427524903 38-39 0.0023777653005144076 40-41 0.0036747281917040853 42-43 0.003917908733802149 44-45 0.004944671022660644 46-47 0.004971691082893762 48-49 0.0021616048186494617 50-51 0.001702263794686451 52-53 0.0010537823490916127 54-55 0.005512092287556128 56-57 9.457021081591396E-4 58-59 9.727221683922577E-4 60-61 8.106018069935481E-5 62-63 9.457021081591396E-4 64-65 0.0 66-67 0.003458567709839139 68-69 6.484814455948385E-4 70-71 0.0 72-73 0.0022426649993488165 74-75 0.005268911745458063 76-77 0.0018373640958520426 78-79 1.8914042163182792E-4 80-81 0.0 82-83 0.005458052167089891 84-85 0.0018373640958520426 86-87 0.0 88-89 0.0010267622888584943 90-91 9.727221683922577E-4 92-93 0.0 94-95 0.0033234674086735473 96-97 0.0015671634935208597 98-99 7.295416262941934E-4 100-101 0.0 102-103 0.0014590832525883867 104-105 0.0 106-107 8.91661987692903E-4 108-109 0.002566905722146236 110-111 5.4040120466236546E-5 112-113 1.8914042163182792E-4 114-115 0.0 116-117 0.001999484457250752 118-119 0.0026479659028455908 120-121 0.0030802868665754826 122-123 0.001783323975385806 124-125 0.09781261804388815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1850477.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.85969304059103 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4226792914074 48.336398821363616 2 11.12992174054186 13.547272413903567 3 3.646680329010378 6.658075363221993 4 1.7585020711270452 4.280875850401422 5 0.9997271073785501 3.0421542439708276 6 0.6730202086552004 2.4575881985322017 7 0.4551463123430728 1.9390045400429465 8 0.32296004903430153 1.5724199558881455 9 0.260574737771061 1.427264869939135 >10 1.2677570444065702 13.270620055768864 >50 0.05047108999213849 2.011282936965981 >100 0.012025772335494917 1.1092508232091693 >500 2.6712299844185806E-4 0.10041421632062397 >1k 2.671229984418569E-4 0.24737771047147697 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.10667519780035095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4040120466236546E-5 2 0.0 0.0 0.0 0.0 5.4040120466236546E-5 3 0.0 0.0 0.0 0.0 5.4040120466236546E-5 4 0.0 0.0 0.0 5.4040120466236546E-5 5.4040120466236546E-5 5 0.0 0.0 0.0 5.4040120466236546E-5 5.4040120466236546E-5 6 0.0 0.0 0.0 5.4040120466236546E-5 5.4040120466236546E-5 7 0.0 0.0 0.0 5.4040120466236546E-5 5.4040120466236546E-5 8 0.0 0.0 0.0 5.4040120466236546E-5 5.4040120466236546E-5 9 0.0 0.0 0.0 1.0808024093247309E-4 1.0808024093247309E-4 10-11 0.0 0.0 0.0 1.0808024093247309E-4 1.0808024093247309E-4 12-13 0.0 0.0 0.0 1.0808024093247309E-4 2.1616048186494618E-4 14-15 0.0 0.0 0.0 1.0808024093247309E-4 3.782808432636558E-4 16-17 0.0 0.0 0.0 1.0808024093247309E-4 4.863610841961289E-4 18-19 0.0 0.0 0.0 1.0808024093247309E-4 4.863610841961289E-4 20-21 0.0 0.0 0.0 2.702006023311827E-4 5.404012046623654E-4 22-23 0.0 0.0 0.0 4.863610841961289E-4 6.214613853617202E-4 24-25 0.0 0.0 0.0 9.99742228625376E-4 7.295416262941934E-4 26-27 0.0 0.0 0.0 0.0015131233730546231 8.106018069935481E-4 28-29 0.0 0.0 0.0 0.0038638686133359128 8.106018069935481E-4 30-31 0.0 0.0 0.0 0.014482752284951394 8.106018069935481E-4 32-33 0.0 0.0 0.0 0.03636900107377719 8.106018069935481E-4 34-35 0.0 0.0 0.0 0.06495622480041632 8.646419274597847E-4 36-37 0.0 0.0 0.0 0.10591863611382363 8.646419274597847E-4 38-39 0.0 0.0 0.0 0.17303646573288942 8.646419274597847E-4 40-41 0.0 0.0 0.0 0.2525565029989565 8.646419274597847E-4 42-43 0.0 0.0 0.0 0.3295906947235767 8.916619876929029E-4 44-45 0.0 0.0 0.0 0.414649844337433 9.186820479260212E-4 46-47 0.0 0.0 0.0 0.5079771323826235 9.186820479260212E-4 48-49 0.0 0.0 0.0 0.601520580909679 0.0010267622888584943 50-51 0.0 0.0 0.0 0.7004680414833581 0.0010808024093247309 52-53 0.0 0.0 0.0 0.8073323797053409 0.0012159027104903222 54-55 0.0 2.7020060233118273E-5 0.0 0.9292739115374036 0.00140504313212215 56-57 0.0 5.4040120466236546E-5 0.0 1.0608616048726895 0.0015401434332877415 58-59 0.0 5.4040120466236546E-5 0.0 1.1958268057371153 0.0016212036139870963 60-61 0.0 5.4040120466236546E-5 0.0 1.329846304493382 0.0016752437344533329 62-63 0.0 5.4040120466236546E-5 0.0 1.481536922642108 0.0018373640958520424 64-65 0.0 5.4040120466236546E-5 0.0 1.634632583922956 0.0018643841560851606 66-67 2.7020060233118273E-5 5.4040120466236546E-5 0.0 1.811884179052212 0.0019184242765513974 68-69 5.4040120466236546E-5 5.4040120466236546E-5 0.0 2.0035104462254867 0.0019454443367845156 70-71 5.4040120466236546E-5 5.4040120466236546E-5 0.0 2.2085927033948543 0.001999484457250752 72-73 5.4040120466236546E-5 5.4040120466236546E-5 0.0 2.399759629544166 0.0021886248788825797 74-75 5.4040120466236546E-5 5.4040120466236546E-5 0.0 2.600896957919499 0.002215644939115698 76-77 5.4040120466236546E-5 5.4040120466236546E-5 0.0 2.838619447850473 0.002215644939115698 78-79 5.4040120466236546E-5 5.4040120466236546E-5 0.0 3.101551653978947 0.002215644939115698 80-81 5.4040120466236546E-5 8.106018069935483E-5 0.0 3.3563508219772524 0.0022967051198150533 82-83 5.4040120466236546E-5 1.0808024093247309E-4 0.0 3.6198504493706216 0.0026479659028455908 84-85 5.4040120466236546E-5 1.0808024093247309E-4 0.0 3.9003456946506225 0.0026479659028455908 86-87 5.4040120466236546E-5 1.0808024093247309E-4 0.0 4.21937154582305 0.0026479659028455908 88-89 5.4040120466236546E-5 1.0808024093247309E-4 0.0 4.570362128251256 0.0026479659028455908 90-91 5.4040120466236546E-5 1.0808024093247309E-4 0.0 4.93172841380898 0.0026479659028455908 92-93 5.4040120466236546E-5 1.0808024093247309E-4 0.0 5.289960372379662 0.0026479659028455908 94-95 5.4040120466236546E-5 1.0808024093247309E-4 0.0 5.665944510523502 0.0026479659028455908 96-97 5.4040120466236546E-5 1.0808024093247309E-4 0.0 6.088051891485277 0.002702006023311827 98-99 5.4040120466236546E-5 1.0808024093247309E-4 0.0 6.558363059902932 0.002702006023311827 100-101 5.4040120466236546E-5 1.0808024093247309E-4 0.0 7.047912511206571 0.002702006023311827 102-103 5.4040120466236546E-5 1.0808024093247309E-4 0.0 7.5425417338340335 0.002783066204011182 104-105 5.4040120466236546E-5 1.0808024093247309E-4 0.0 8.043655770917445 0.0028641263847105367 106-107 5.4040120466236546E-5 1.0808024093247309E-4 0.0 8.584597376784473 0.002891146444943655 108-109 5.4040120466236546E-5 1.0808024093247309E-4 0.0 9.185361396007625 0.0029992266858761283 110-111 5.4040120466236546E-5 1.0808024093247309E-4 0.0 9.817009344077231 0.00313432698704172 112-113 5.4040120466236546E-5 1.0808024093247309E-4 0.0 10.440254053414336 0.003188367107507956 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 52.448933 1 GTATCAA 4110 0.0 49.4969 1 TCAACGC 5195 0.0 38.568336 4 CAACGCA 5330 0.0 37.65642 5 TATCAAC 5515 0.0 36.55167 2 ATCAACG 5530 0.0 36.34369 3 GTACATG 9110 0.0 36.213642 1 AACGCAG 5680 0.0 35.359 6 TACATGG 9485 0.0 34.582893 2 ACATGGG 9750 0.0 33.458538 3 CATGGGG 8400 0.0 32.797436 4 ATGGGGG 4930 0.0 30.443174 5 ACGCAGA 6895 0.0 29.12665 7 CGCAGAG 7360 0.0 27.28497 8 GTATAGG 455 0.0 24.911945 1 GCAGAGT 8195 0.0 24.577581 9 ATGGGGA 2995 0.0 24.061619 5 TGGGGGG 3310 0.0 23.406355 6 AGAGTAC 7440 0.0 22.70657 10-11 AGTACAT 6710 0.0 20.798372 2 >>END_MODULE