FastQCFastQC Report
Mon 15 Aug 2016
SRR2921631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954466
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.044.120781
GGTATCA19900.039.48281
GTACATG89700.038.0896071
CAACGCA33800.037.8508225
TCAACGC33750.037.734454
TACATGG93200.036.0140842
ACATGGG95000.034.8296623
AACGCAG38500.033.5357556
ATCAACG38850.032.78093
CATGGGG87750.032.553094
TATCAAC40850.032.342242
ATGGGGG54300.031.0127455
ACGCAGA45500.028.3713267
CGCAGAG50250.025.5710748
GAGTACA62900.025.266651
TAGATCG1451.3397768E-424.6227665
AGTACAT62950.024.485742
GCAGAGT58600.021.4198369
AGAGTAC50050.020.14611610-11
GTATAGG4750.020.0499551
Produced by FastQC (version 0.11.5)