##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921631_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1954466 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.126926229466257 31.0 31.0 34.0 26.0 34.0 2 30.23624458036108 31.0 31.0 34.0 26.0 34.0 3 30.4198205545658 31.0 31.0 34.0 27.0 34.0 4 33.95911926838328 37.0 35.0 37.0 30.0 37.0 5 33.93460157403608 37.0 35.0 37.0 30.0 37.0 6 33.918065599503905 37.0 35.0 37.0 30.0 37.0 7 33.75213945906452 37.0 35.0 37.0 29.0 37.0 8 33.797785686729775 37.0 35.0 37.0 30.0 37.0 9 35.42161183668583 39.0 37.0 39.0 30.0 39.0 10-11 35.47317783988056 39.0 37.0 39.0 30.0 39.0 12-13 35.437398757512284 39.0 37.0 39.0 30.0 39.0 14-15 36.728728460868595 40.0 38.0 41.0 30.0 41.0 16-17 36.67818370849122 40.0 38.0 41.0 29.5 41.0 18-19 36.623033861934665 40.0 38.0 41.0 29.0 41.0 20-21 36.67690356342858 40.0 38.0 41.0 29.0 41.0 22-23 36.59412596586485 40.0 38.0 41.0 29.0 41.0 24-25 36.56933505110859 40.0 38.0 41.0 29.0 41.0 26-27 36.52235521109091 40.0 38.0 41.0 29.0 41.0 28-29 36.45798571067493 40.0 38.0 41.0 29.0 41.0 30-31 36.36471931463633 40.0 38.0 41.0 28.5 41.0 32-33 36.274511298738375 40.0 37.0 41.0 28.0 41.0 34-35 36.14436475231598 40.0 37.0 41.0 27.5 41.0 36-37 36.04112427640082 40.0 37.0 41.0 27.0 41.0 38-39 35.922305376506934 40.0 37.0 41.0 27.0 41.0 40-41 35.814342127210196 40.0 36.0 41.0 26.0 41.0 42-43 35.5605423680944 40.0 35.5 41.0 25.5 41.0 44-45 35.19267308819903 39.5 35.0 41.0 24.0 41.0 46-47 34.933987595588775 39.0 35.0 41.0 24.0 41.0 48-49 34.734745705476584 39.0 35.0 41.0 23.0 41.0 50-51 34.52530767994941 39.0 34.5 40.0 23.0 41.0 52-53 34.31218194637307 38.0 34.0 40.0 22.0 41.0 54-55 34.0629865139634 38.0 34.0 40.0 21.5 41.0 56-57 33.80245192292933 38.0 34.0 40.0 20.0 41.0 58-59 33.809461254378434 38.0 34.0 40.0 20.5 41.0 60-61 33.59358694395298 37.0 34.0 40.0 20.0 41.0 62-63 33.281757011889695 37.0 33.5 40.0 18.5 41.0 64-65 32.9802785006237 36.0 33.0 39.0 18.0 41.0 66-67 32.66252393236823 36.0 33.0 39.0 17.5 41.0 68-69 32.32680461056882 35.0 33.0 39.0 14.0 40.0 70-71 31.96504902106253 35.0 32.5 37.5 12.0 40.0 72-73 31.616587344062268 35.0 32.0 37.0 9.0 39.0 74-75 31.25294198006003 35.0 32.0 36.5 7.5 39.0 76-77 30.961641440679962 35.0 32.0 36.0 7.0 38.5 78-79 30.69511672241932 35.0 31.5 36.0 4.0 37.0 80-81 30.436657890185863 35.0 31.0 35.0 2.0 37.0 82-83 30.214688564549093 35.0 31.0 35.0 2.0 36.5 84-85 29.99827574386047 35.0 31.0 35.0 2.0 36.0 86-87 29.823832443235133 34.5 31.0 35.0 2.0 36.0 88-89 29.65956174218431 34.0 31.0 35.0 2.0 35.5 90-91 29.46720587618306 34.0 30.5 35.0 2.0 35.0 92-93 29.384566679594325 34.0 30.0 35.0 2.0 35.0 94-95 29.246861802661186 34.0 30.0 35.0 2.0 35.0 96-97 29.15138661915838 34.0 30.0 35.0 2.0 35.0 98-99 29.04270680584876 34.0 30.0 35.0 2.0 35.0 100-101 28.91823060621162 34.0 30.0 35.0 2.0 35.0 102-103 28.012290825217733 33.0 28.0 34.5 2.0 35.0 104-105 28.963259529712975 34.0 30.0 35.0 2.0 35.0 106-107 29.248549220093878 34.0 31.0 35.0 2.0 35.0 108-109 29.24297071425136 35.0 31.0 35.0 2.0 35.0 110-111 29.168037714649422 35.0 31.0 35.0 2.0 35.0 112-113 29.066170759685768 34.0 31.0 35.0 2.0 35.0 114-115 28.928861643026792 34.0 31.0 35.0 2.0 35.0 116-117 28.786271800072242 34.0 30.0 35.0 2.0 35.0 118-119 28.472778753889813 34.0 30.0 35.0 2.0 35.0 120-121 28.31692288328372 34.0 30.0 35.0 2.0 35.0 122-123 28.032370222863943 34.0 29.5 35.0 2.0 35.0 124-125 27.204914539316622 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77450.0 3 14351.0 4 6981.0 5 6213.0 6 6613.0 7 7085.0 8 7102.0 9 7418.0 10 7904.0 11 7926.0 12 7739.0 13 7721.0 14 7514.0 15 6905.0 16 6409.0 17 5997.0 18 5770.0 19 5600.0 20 5500.0 21 5888.0 22 6629.0 23 8748.0 24 11960.0 25 14733.0 26 17127.0 27 19754.0 28 23898.0 29 28471.0 30 34742.0 31 44159.0 32 57821.0 33 80093.0 34 124591.0 35 204162.0 36 397880.0 37 545710.0 38 119864.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.42177044817748 16.785302132777215 11.164442624838475 25.62848479420683 2 17.147230065590918 19.342770620099742 37.143607162704164 26.366392151605183 3 20.307880298792487 22.746903440985005 28.361696923550838 28.58351933667167 4 13.965618259830572 14.439000427277172 33.94479169598334 37.65058961690892 5 15.00116931173339 37.51187862083647 32.44571399914336 15.041238068286782 6 35.758397318143246 33.03966810691169 16.333372083555037 14.868562491390025 7 31.50166178949028 30.946093719602974 19.042745771805482 18.509498719101263 8 28.221312067030013 34.493806798316676 18.686540606589258 18.598340528064057 9 28.139280302196717 15.541114219899189 17.874428276130665 38.445177201773426 10-11 27.117530885937803 24.986664810148874 26.112170190808392 21.78363411310493 12-13 26.766808794802028 22.329391868121466 26.37046191004351 24.533337427033004 14-15 24.294375165690692 23.813040509902546 23.37600092530894 28.516583399097822 16-17 24.98882303700669 26.81009860039088 24.11680239820391 24.08427596439853 18-19 25.026795805215222 26.173436169167267 25.311389266526895 23.48837875909061 20-21 25.499544374075413 25.184457487836543 25.184150410549538 24.13184772753851 22-23 25.64124809308875 24.72905289498623 24.91666660268709 24.713032409237933 24-25 25.1170567791594 25.16578085578825 24.98032739815734 24.73683496689501 26-27 24.751524712903002 25.378327667195016 25.21344956872425 24.65669805117773 28-29 24.880221252844745 25.644834515935177 25.082360685543936 24.392583545676143 30-31 25.357799216872003 25.270258997246103 24.99599531003465 24.37594647584724 32-33 24.8706557926603 25.315101374434146 25.028724675583895 24.785518157321658 34-35 24.990562350805163 25.519992076213722 25.20153060114562 24.287914971835495 36-37 25.030266901365213 25.502384509896224 25.09238696604376 24.3749616226948 38-39 25.072054884533074 25.556506279726477 25.085846639775728 24.28559219596472 40-41 25.023603333975725 25.404225489450866 25.085334146173548 24.486837030399865 42-43 24.92217433869298 25.24511948791406 25.054335949954122 24.77837022343884 44-45 24.72978325347001 25.303606847299008 25.278864910560582 24.687744988670392 46-47 24.71189588462171 25.303930426665584 25.29998984147845 24.68418384723426 48-49 24.643894255325467 25.53208585434159 25.212803634141522 24.61121625619142 50-51 24.670087807694138 25.48928338747211 25.32336128897212 24.51726751586163 52-53 24.861743864580124 25.382449787152595 25.21381977930196 24.541986568965324 54-55 24.599875182090088 25.118286591439865 25.398320869027422 24.88351735744262 56-57 24.57106477471417 25.367868042684847 25.13452564245926 24.926541540141724 58-59 24.58384357666546 25.476131870246295 25.2578765109642 24.68214804212405 60-61 24.863998182207123 25.31926522683005 25.317499634087305 24.499236956875524 62-63 24.669259266651416 25.34583779208331 25.412930144629826 24.571972796635457 64-65 24.567588528663627 25.122174455834628 25.345042566273833 24.965194449227905 66-67 24.569340757030854 25.430633653424415 25.166319245947765 24.83370634359696 68-69 24.482611975357795 25.35938253244806 25.278652600808783 24.879352891385363 70-71 24.379797770908894 25.208022311898205 25.553823973619664 24.858355943573233 72-73 24.51768774655517 25.449726094591725 25.249808026967575 24.78277813188554 74-75 24.370723989460107 25.39613501479694 25.30994000632967 24.92320098941329 76-77 24.407914229760134 25.072269236103047 25.428997042705504 25.090819491431315 78-79 24.29792229831739 25.087436655124446 25.591252956776557 25.023388089781605 80-81 24.390419267702246 25.36597872330082 25.312962246997927 24.930639761999007 82-83 24.420445833729957 25.34518900185363 25.3881009333665 24.846264231049915 84-85 24.41673423365222 25.339781556298156 25.26755374933446 24.97593046071516 86-87 24.23673468134527 25.458987778236637 25.379361835940877 24.92491570447722 88-89 24.314430016719285 25.127979034109487 25.482272287389502 25.075318661781722 90-91 24.411613889750335 25.047388174654593 25.468482769843924 25.072515165751142 92-93 24.38857842260614 25.185352336377104 25.561468102563627 24.864601138453125 94-95 24.407152597793022 24.9481515974118 25.59507331438323 25.04962249041195 96-97 24.265277434495307 24.91790644942899 25.599345605532363 25.217470510543333 98-99 24.14183041383919 25.02041616223225 25.525933642867592 25.31181978106096 100-101 24.118291498530912 25.075936127687314 25.65986141144998 25.14591096233179 102-103 23.954005991654665 24.983460270110548 25.784684184538868 25.27784955369592 104-105 23.90561312987942 25.01921323466958 25.759830984935387 25.31534265051561 106-107 23.882391616066393 25.008640527206467 25.76719055592295 25.341777300804193 108-109 23.88403508453227 25.042338416160725 25.79942784610232 25.27419865320468 110-111 23.72927448182847 25.333131741525534 25.718637699868175 25.218956076777825 112-113 23.65498061192981 25.367940981061675 25.783408764784095 25.19366964222442 114-115 23.61871968244977 25.438303601377644 25.70690312021146 25.236073595961134 116-117 23.482048608215166 25.27483407620189 25.832714631534298 25.41040268404865 118-119 23.5671644471353 25.229106432322467 25.796453025549198 25.407276094993037 120-121 23.407882070057102 25.36081150257988 25.93150957930076 25.299796848062257 122-123 23.420148149285154 25.421762416232717 25.83970661785492 25.318382816627206 124-125 23.291398801503895 25.318448370994854 25.936199971769895 25.453952855731355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 652.0 1 522.0 2 326.0 3 234.5 4 181.5 5 160.0 6 177.5 7 207.5 8 250.0 9 256.0 10 244.5 11 265.0 12 279.5 13 312.5 14 363.5 15 394.0 16 424.0 17 474.0 18 515.5 19 556.5 20 625.0 21 668.0 22 780.5 23 989.0 24 1166.0 25 1416.5 26 1903.0 27 2557.0 28 3460.0 29 4691.0 30 6462.0 31 8680.0 32 11843.0 33 14775.0 34 17425.5 35 21187.5 36 25568.0 37 29889.0 38 34172.5 39 38485.0 40 42354.5 41 46295.5 42 50171.5 43 55975.0 44 62461.5 45 68536.0 46 72976.5 47 76658.5 48 82657.5 49 86904.0 50 88371.5 51 88852.5 52 88557.5 53 87711.5 54 88108.5 55 86063.0 56 80064.5 57 73364.5 58 67816.5 59 61918.5 60 53128.0 61 44857.0 62 37459.5 63 29183.0 64 22114.0 65 17094.0 66 13180.5 67 10484.5 68 8386.0 69 6647.5 70 5285.5 71 4079.5 72 3152.0 73 2585.5 74 2099.5 75 1685.5 76 1254.0 77 789.0 78 512.5 79 343.0 80 232.5 81 165.0 82 131.0 83 104.0 84 80.5 85 65.0 86 56.5 87 44.0 88 36.0 89 31.0 90 26.0 91 22.0 92 24.5 93 21.5 94 16.0 95 10.5 96 11.0 97 13.0 98 7.0 99 6.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022563707938638995 2 0.011870249981324821 3 0.010232974121831743 4 0.011819085110715664 5 0.016628582947976582 6 0.09153395351978494 7 0.058839601200532526 8 0.048760121690528256 9 0.02594058939884347 10-11 0.05055089216184881 12-13 0.012458645993330148 14-15 0.026810392199199166 16-17 0.03430604574344092 18-19 0.041034226228545294 20-21 0.028677909976433463 22-23 0.03671079466207138 24-25 0.03103149402445476 26-27 0.04612513085415658 28-29 0.04420644820631313 30-31 0.02594058939884347 32-33 0.029215141117829625 34-35 0.04372038193552612 36-37 0.00951666593330352 38-39 0.020465948243663486 40-41 0.04208310607603304 42-43 0.021438080785237502 44-45 0.015042471959092663 46-47 0.022589290373943575 48-49 0.02824300857625561 50-51 0.02747553551711823 52-53 0.02476379737483282 54-55 0.021463663220542082 56-57 0.024073071621609175 58-59 0.017037901912849852 60-61 0.022921862032903105 62-63 0.022666037679857313 64-65 0.02023570632592227 66-67 0.027782524740773182 68-69 0.02148924565584666 70-71 0.04551115240684668 72-73 0.021259003738105447 74-75 0.009030599662516514 76-77 0.016116934241884998 78-79 0.024559137892396183 80-81 0.018240276372165083 82-83 0.023740499962649642 84-85 0.011895832416629401 86-87 0.016833242430413217 88-89 0.022256718714984042 90-91 0.020056629278790216 92-93 0.007546818414850911 94-95 0.01079578769853249 96-97 0.014991307088483502 98-99 0.00675376292040895 100-101 0.009823655156958475 102-103 0.004144354519341855 104-105 0.004297849131169333 106-107 6.907257532236426E-4 108-109 0.013891262370386591 110-111 0.0011000447180969123 112-113 0.0021745070008892453 114-115 0.01307262444064005 116-117 0.005372311413961665 118-119 0.2804346558087989 120-121 8.953852356602776E-4 122-123 9.465501062694362E-4 124-125 0.3167617139413016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1954466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.53115866594809 #Duplication Level Percentage of deduplicated Percentage of total 1 81.31365831110973 50.033236112495814 2 10.195183161276168 12.54642865449772 3 3.1987915701451515 5.904760549256955 4 1.5921727491663387 3.91872936210211 5 0.9127847169312518 2.8082350622674683 6 0.5687855163521206 2.0998819112133327 7 0.3873796731892772 1.6685144094480768 8 0.29621945495337065 1.4581381026141211 9 0.2306712792549106 1.2774123975160012 >10 1.2172027679283504 13.10255006033918 >50 0.06419597838174186 2.6747095136397063 >100 0.022206733497800907 2.217308101364298 >500 7.480878137653449E-4 0.29009576324519076 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.116487060915872E-5 0.0 5 0.0 0.0 0.0 5.116487060915872E-5 0.0 6 0.0 0.0 0.0 5.116487060915872E-5 1.5349461182747614E-4 7 0.0 0.0 0.0 5.116487060915872E-5 1.5349461182747614E-4 8 0.0 0.0 0.0 5.116487060915872E-5 1.5349461182747614E-4 9 0.0 0.0 0.0 5.116487060915872E-5 2.0465948243663488E-4 10-11 0.0 0.0 0.0 5.116487060915872E-5 2.0465948243663488E-4 12-13 0.0 0.0 0.0 1.0232974121831744E-4 2.558243530457936E-4 14-15 0.0 0.0 0.0 1.279121765228968E-4 3.325716589595317E-4 16-17 0.0 0.0 0.0 1.5349461182747614E-4 4.6048383548242844E-4 18-19 0.0 0.0 0.0 1.5349461182747614E-4 5.116487060915872E-4 20-21 0.0 0.0 0.0 1.5349461182747614E-4 5.372311413961665E-4 22-23 2.558243530457936E-5 0.0 0.0 3.0698922365495233E-4 6.39560882614484E-4 24-25 5.116487060915872E-5 0.0 0.0 4.604838354824285E-4 7.16308188528222E-4 26-27 5.116487060915872E-5 0.0 0.0 0.0011767920240106505 7.16308188528222E-4 28-29 5.116487060915872E-5 0.0 0.0 0.005218816802134189 7.16308188528222E-4 30-31 1.0232974121831744E-4 0.0 0.0 0.01987755223165816 7.16308188528222E-4 32-33 1.0232974121831744E-4 0.0 0.0 0.04753216479590845 7.674730591373807E-4 34-35 1.0232974121831744E-4 0.0 0.0 0.0832708269164058 8.186379297465395E-4 36-37 1.0232974121831744E-4 0.0 0.0 0.13543341250244312 8.186379297465395E-4 38-39 1.0232974121831744E-4 0.0 0.0 0.21420173080524296 8.953852356602776E-4 40-41 1.0232974121831744E-4 0.0 0.0 0.30645199251355615 9.721325415740156E-4 42-43 1.0232974121831744E-4 0.0 0.0 0.39760220950377234 9.97714976878595E-4 44-45 1.0232974121831744E-4 0.0 0.0 0.501569226581583 0.0010232974121831744 46-47 1.0232974121831744E-4 0.0 0.0 0.6153087339457427 0.0010488798474877535 48-49 1.0232974121831744E-4 0.0 0.0 0.7358531691009207 0.001074462282792333 50-51 1.0232974121831744E-4 0.0 0.0 0.8514090293717056 0.001074462282792333 52-53 1.0232974121831744E-4 0.0 0.0 0.9757652473872659 0.0011512095887060711 54-55 1.0232974121831744E-4 2.558243530457936E-5 0.0 1.1169291253979348 0.001227956894619809 56-57 1.0232974121831744E-4 5.116487060915872E-5 0.0 1.269502769554446 0.0012535393299243885 58-59 1.0232974121831744E-4 5.116487060915872E-5 0.0 1.4263998452774314 0.001279121765228968 60-61 1.0232974121831744E-4 5.116487060915872E-5 0.0 1.58795292422585 0.0013302866358381267 62-63 1.0232974121831744E-4 7.674730591373808E-5 0.0 1.7547248199764027 0.0013814515064472853 64-65 1.0232974121831744E-4 1.5349461182747614E-4 0.0 1.9343390982498545 0.0013814515064472853 66-67 1.0232974121831744E-4 1.5349461182747614E-4 0.0 2.136517084461945 0.0014070339417518647 68-69 1.0232974121831744E-4 1.5349461182747614E-4 0.0 2.3588284472587397 0.001432616377056444 70-71 1.0232974121831744E-4 1.5349461182747614E-4 0.0 2.5894541015295225 0.0015093636829701823 72-73 1.0232974121831744E-4 1.5349461182747614E-4 0.0 2.812149200855886 0.001790770471320555 74-75 1.0232974121831744E-4 1.5349461182747614E-4 0.0 3.050782157377002 0.001790770471320555 76-77 1.0232974121831744E-4 1.5349461182747614E-4 0.0 3.332623847127553 0.001790770471320555 78-79 1.0232974121831744E-4 1.5349461182747614E-4 0.0 3.641403841253826 0.001790770471320555 80-81 1.0232974121831744E-4 1.5349461182747614E-4 0.0 3.945630161895884 0.0018163529066251344 82-83 1.0232974121831744E-4 1.5349461182747614E-4 0.0 4.252030989538831 0.00199542995375719 84-85 1.0232974121831744E-4 1.5349461182747614E-4 0.0 4.577797720707345 0.00199542995375719 86-87 1.0232974121831744E-4 2.0465948243663488E-4 0.0 4.941221796644198 0.0020210123890617693 88-89 1.0232974121831744E-4 2.0465948243663488E-4 0.0 5.345935923162644 0.0020465948243663487 90-91 1.0232974121831744E-4 2.0465948243663488E-4 0.0 5.764106410651298 0.0020465948243663487 92-93 1.0232974121831744E-4 2.0465948243663488E-4 0.0 6.18332577798744 0.002148924565584666 94-95 1.0232974121831744E-4 2.0465948243663488E-4 0.0 6.624315797767779 0.0022000894361938247 96-97 1.0232974121831744E-4 2.0465948243663488E-4 0.0 7.100507248527219 0.002276836742107563 98-99 1.0232974121831744E-4 2.0465948243663488E-4 0.0 7.636152278934501 0.0023024191774121423 100-101 1.0232974121831744E-4 2.0465948243663488E-4 0.0 8.2042870021786 0.0023024191774121423 102-103 1.0232974121831744E-4 2.0465948243663488E-4 0.0 8.774596232423587 0.0023280016127167217 104-105 1.0232974121831744E-4 2.0465948243663488E-4 0.0 9.36191778214612 0.00240474891863046 106-107 1.0232974121831744E-4 2.0465948243663488E-4 0.0 9.997462222417784 0.002481496224544198 108-109 1.0232974121831744E-4 2.0465948243663488E-4 0.0 10.671764052175888 0.002583825965762515 110-111 1.0232974121831744E-4 2.0465948243663488E-4 0.0 11.381164983171875 0.0026861557069808324 112-113 1.0232974121831744E-4 2.0465948243663488E-4 0.0 12.09698710542931 0.0027629030128945705 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2995 0.0 44.12078 1 GGTATCA 1990 0.0 39.4828 1 GTACATG 8970 0.0 38.089607 1 CAACGCA 3380 0.0 37.850822 5 TCAACGC 3375 0.0 37.73445 4 TACATGG 9320 0.0 36.014084 2 ACATGGG 9500 0.0 34.829662 3 AACGCAG 3850 0.0 33.535755 6 ATCAACG 3885 0.0 32.7809 3 CATGGGG 8775 0.0 32.55309 4 TATCAAC 4085 0.0 32.34224 2 ATGGGGG 5430 0.0 31.012745 5 ACGCAGA 4550 0.0 28.371326 7 CGCAGAG 5025 0.0 25.571074 8 GAGTACA 6290 0.0 25.26665 1 TAGATCG 145 1.3397768E-4 24.622766 5 AGTACAT 6295 0.0 24.48574 2 GCAGAGT 5860 0.0 21.419836 9 AGAGTAC 5005 0.0 20.146116 10-11 GTATAGG 475 0.0 20.049955 1 >>END_MODULE