FastQCFastQC Report
Mon 15 Aug 2016
SRR2921631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954466
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.120237445931523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.056.398381
GTATCAA40800.052.191241
TCAACGC49000.042.5952574
CAACGCA49650.041.980555
ATCAACG52400.039.9513973
AACGCAG53450.039.132446
TATCAAC54750.038.3455242
GTACATG94700.034.956871
TACATGG97750.033.4974482
ACATGGG97650.033.1042063
ATGGGGG50700.032.1841435
CATGGGG86250.031.8735984
ACGCAGA67100.031.0830067
CGCAGAG73500.028.3750048
GCAGAGT81300.025.2855649
AGTACAT66650.023.0874352
GAGTACA67800.022.9615041
TGGGGGG37150.021.9755256
TAACGCT1653.1680847E-421.6848584
AGAGTAC73300.021.6232910-11
Produced by FastQC (version 0.11.5)