##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921631_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1954466 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77922767651113 33.0 31.0 34.0 30.0 34.0 2 31.951692175765658 33.0 31.0 34.0 30.0 34.0 3 32.03224819464754 34.0 31.0 34.0 30.0 34.0 4 35.43854433896522 37.0 35.0 37.0 35.0 37.0 5 35.30654204268583 37.0 35.0 37.0 33.0 37.0 6 35.34293766174495 37.0 35.0 37.0 33.0 37.0 7 35.23054941861358 37.0 35.0 37.0 33.0 37.0 8 35.261721616032204 37.0 36.0 37.0 33.0 37.0 9 36.985168838956525 39.0 38.0 39.0 34.0 39.0 10-11 36.93930797465906 39.0 38.0 39.0 34.0 39.0 12-13 36.86940115612141 39.0 38.0 39.0 34.0 39.0 14-15 38.30625244951818 41.0 39.0 41.0 34.0 41.0 16-17 38.31586632870564 41.0 39.0 41.0 34.0 41.0 18-19 38.31361302780401 41.0 39.0 41.0 34.0 41.0 20-21 38.24908389299175 41.0 39.0 41.0 34.0 41.0 22-23 38.2528936292573 41.0 39.0 41.0 34.0 41.0 24-25 38.21139917501763 41.0 39.0 41.0 34.0 41.0 26-27 38.14366532853475 41.0 39.0 41.0 34.0 41.0 28-29 38.072740329071976 41.0 39.0 41.0 33.5 41.0 30-31 37.96760368305205 41.0 38.0 41.0 33.0 41.0 32-33 37.85936184103484 41.0 38.0 41.0 33.0 41.0 34-35 37.731761258573954 41.0 38.0 41.0 33.0 41.0 36-37 37.655968944970134 40.5 38.0 41.0 33.0 41.0 38-39 37.57393144725977 40.0 38.0 41.0 33.0 41.0 40-41 37.45410408776617 40.0 38.0 41.0 32.0 41.0 42-43 37.29872328298369 40.0 38.0 41.0 32.0 41.0 44-45 37.155729749200034 40.0 37.0 41.0 31.5 41.0 46-47 36.98122837644656 40.0 37.0 41.0 31.0 41.0 48-49 36.80169186877643 40.0 37.0 41.0 31.0 41.0 50-51 36.55657171831078 40.0 36.0 41.0 30.0 41.0 52-53 36.42659222519092 40.0 36.0 41.0 30.0 41.0 54-55 36.29318545321331 40.0 35.0 41.0 30.0 41.0 56-57 36.091971668987846 39.0 35.0 41.0 30.0 41.0 58-59 35.89473672092531 39.0 35.0 41.0 30.0 41.0 60-61 35.638318599556094 38.5 35.0 41.0 29.5 41.0 62-63 35.28425181098059 37.5 35.0 40.5 29.0 41.0 64-65 34.99239127209171 37.0 35.0 40.0 29.0 41.0 66-67 34.69184447311952 36.5 35.0 39.5 29.0 41.0 68-69 34.32163567951553 36.0 35.0 39.0 28.5 41.0 70-71 33.9491971208504 35.5 35.0 39.0 28.0 41.0 72-73 33.63481610833854 35.0 34.0 37.5 28.0 40.0 74-75 33.31883235625486 35.0 34.0 37.0 28.0 39.0 76-77 33.00097673737993 35.0 34.0 36.5 27.0 39.0 78-79 32.74757887832277 35.0 34.0 36.0 27.0 38.0 80-81 32.50025991754269 35.0 34.0 36.0 26.5 37.0 82-83 32.28438023480582 35.0 34.0 35.0 26.0 37.0 84-85 32.07367357631189 35.0 34.0 35.0 26.0 36.0 86-87 31.95134630124034 35.0 34.0 35.0 26.0 36.0 88-89 31.86117768229276 35.0 34.0 35.0 26.0 36.0 90-91 31.76091935086105 35.0 34.0 35.0 25.0 35.5 92-93 31.684409705771294 35.0 34.0 35.0 25.0 35.0 94-95 31.621348746921157 35.0 33.0 35.0 25.0 35.0 96-97 31.55417362082533 35.0 33.0 35.0 25.0 35.0 98-99 31.479091219801212 35.0 33.0 35.0 24.5 35.0 100-101 31.409987178083426 35.0 33.0 35.0 24.0 35.0 102-103 30.710659842637323 34.0 31.5 35.0 23.5 35.0 104-105 31.23968797615308 35.0 33.0 35.0 24.0 35.0 106-107 31.337551279991565 35.0 33.0 35.0 24.0 35.0 108-109 31.320673524123727 35.0 33.0 35.0 24.0 35.0 110-111 31.250743681394304 35.0 33.0 35.0 24.0 35.0 112-113 31.209774434551434 35.0 33.0 35.0 23.5 35.0 114-115 31.074442072668443 35.0 33.0 35.0 23.0 35.0 116-117 31.25605331584177 35.0 33.5 35.0 24.0 35.0 118-119 31.228693157107877 35.0 34.0 35.0 23.5 35.0 120-121 31.139557812722245 35.0 34.0 35.0 21.5 35.0 122-123 30.993782444923575 35.0 33.0 35.0 18.5 35.0 124-125 30.330525575783874 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28885.0 3 7814.0 4 4277.0 5 3386.0 6 3565.0 7 3906.0 8 4495.0 9 4819.0 10 5196.0 11 5505.0 12 6119.0 13 7137.0 14 8781.0 15 8282.0 16 5151.0 17 4486.0 18 4238.0 19 4049.0 20 3994.0 21 3960.0 22 4497.0 23 5546.0 24 7257.0 25 9311.0 26 11223.0 27 13995.0 28 16761.0 29 20275.0 30 24963.0 31 31132.0 32 40824.0 33 55585.0 34 86579.0 35 155026.0 36 359324.0 37 730155.0 38 253655.0 39 313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24748915699646 17.40080091094024 10.639167871444839 27.712542060618468 2 16.395735852049313 19.59149319368513 36.25022065200429 27.762550302261268 3 19.25024157358577 22.76877108650791 28.242511218841106 29.738476121065215 4 13.632573445489438 14.473334225088138 33.66538387225561 38.22870845716682 5 14.772165489768199 37.050193295209944 32.74252996152519 15.435111253496665 6 35.60123698249997 33.192909878452134 16.09403319782504 15.111819941222851 7 31.033340653748947 31.167309378681 19.00323582846884 18.796114139101206 8 28.822364443916644 33.79390162064823 18.483917647462302 18.899816287972826 9 28.170331115794816 14.730082331107605 18.013704449442066 39.085882103655514 10-11 27.273793080145275 24.542435843745743 26.122395276043658 22.061375800065324 12-13 27.04848500982417 21.776710321673683 26.60885475486678 24.56594991363537 14-15 24.339350539050233 23.250499272273224 23.79356825708542 28.616581931591124 16-17 24.715330873469924 26.456516931695788 24.55254579482118 24.275606400013107 18-19 24.74625771719469 25.597977022695794 26.198282895390108 23.457482364719407 20-21 25.346817340777477 24.648973384847686 26.03045979349735 23.973749480877483 22-23 25.117995877531417 24.259016077218128 25.916573863794806 24.706414181455646 24-25 24.67359196962309 24.706134275900425 25.918949190510133 24.701324563966352 26-27 24.41961905561076 24.91925935552797 26.141395390722295 24.519726198138976 28-29 24.493996314949527 25.237274372525754 25.946244927427227 24.322484385097496 30-31 24.965923568508465 24.861980219296676 25.837328530569014 24.334767681625845 32-33 24.61447852710509 24.768472205663304 25.916376238870058 24.700673028361546 34-35 24.645756315957787 24.930732331307965 26.075113527342502 24.34839782539175 36-37 24.68832332403271 24.980108465785793 25.966984402478783 24.36458380770272 38-39 24.793552075991563 25.086476730398033 25.919866559388876 24.200104634221532 40-41 24.77385089854598 24.90194598618763 25.884468822392463 24.439734292873926 42-43 24.613520186594016 24.7852094002083 25.806544042721985 24.794726370475697 44-45 24.466269490511188 24.904976282765816 25.90112314362531 24.727631083097688 46-47 24.582483000376335 24.91176876162612 25.743387358097692 24.762360879899855 48-49 24.45652869101788 25.182342446149182 25.703671715546296 24.657457147286646 50-51 24.54839400411831 25.16373603405372 25.739041050857754 24.54882891097022 52-53 24.746431415668283 25.08355924923182 25.524040888198048 24.645968446901854 54-55 24.35200021285528 24.878944954140174 25.805402686632767 24.963652146371775 56-57 24.398124590838307 25.041987440478884 25.573645011252506 24.9862429574303 58-59 24.41692155456204 25.151858268345023 25.699786231858106 24.731433945234834 60-61 24.642017946110503 24.987483771113542 25.838510587949372 24.531987694826583 62-63 24.520741006610812 24.98793138425935 25.83141697956381 24.659910629566024 64-65 24.337363760740786 24.77548854776701 25.79919527891506 25.087952412577142 66-67 24.31892553879247 25.205875889374376 25.56721560934377 24.907982962489385 68-69 24.3440685593185 25.102541452124594 25.68426416898637 24.869125819570534 70-71 24.16238608458425 24.909546881231723 25.87963307615305 25.04843395803098 72-73 24.356668272432348 25.120093449934267 25.548840561282898 24.974397716350488 74-75 24.14837893306303 25.19549868217796 25.578104556937024 25.078017827821984 76-77 24.14270002770621 24.800792863207814 25.663983283152604 25.39252382593337 78-79 24.075699507460996 24.86371586692928 25.75050608491482 25.310078540694906 80-81 24.15969374755048 25.131570464771453 25.45580224982169 25.252933537856375 82-83 24.20253448245426 25.079776612692573 25.502881117049103 25.214807787804062 84-85 24.15313955740252 25.128687790028803 25.348418306117942 25.369754346450733 86-87 23.913872126708778 25.183502808439744 25.54807809396531 25.354546970886165 88-89 23.841122119354118 24.978580870421236 25.547949813872943 25.632347196351702 90-91 23.986085116354747 24.83606674805342 25.64493715380357 25.532910981788266 92-93 23.9156628971801 25.01368660288795 25.64790075652378 25.422749743408176 94-95 23.92239300679514 24.734683560754732 25.698656603279304 25.644266829170824 96-97 23.73116500604649 24.781644670175428 25.70554079287653 25.781649530901557 98-99 23.666018573000077 24.78717797845941 25.632377395175116 25.914426053365396 100-101 23.57940736753671 24.860754804637175 25.725799272026222 25.8340385557999 102-103 23.486623776246677 24.683719821247845 25.84955368243966 25.98010272006582 104-105 23.523253922043157 24.77369777729569 25.745676824257878 25.957371476403274 106-107 23.53495113988182 24.697985169282312 25.761196076454993 26.005867614380872 108-109 23.44666871842574 24.725999343539428 25.76566813538847 26.06166380264636 110-111 23.300423210552047 24.942100508860612 25.679591519825507 26.077884760761837 112-113 23.178715951575345 24.989805363018615 25.743671266575557 26.08780741883048 114-115 23.169730248569174 25.063597934167188 25.622906717231203 26.14376510003244 116-117 22.971913359081615 24.886533675086067 25.73974722611579 26.401805739716526 118-119 23.104151279547427 24.825069720119227 25.676088806841307 26.39469019349204 120-121 22.875853612424592 24.93616374622681 25.769789366585233 26.418193274763365 122-123 22.778239261090032 24.98750925134047 25.668648387152064 26.565603100417434 124-125 22.616222982588543 24.92095072708429 25.668151062667565 26.794675227659603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20.0 1 21.5 2 27.0 3 56.0 4 68.0 5 72.0 6 181.0 7 398.5 8 651.5 9 755.5 10 607.5 11 501.5 12 516.5 13 530.5 14 572.5 15 612.5 16 646.5 17 686.5 18 715.5 19 760.0 20 818.0 21 878.0 22 962.0 23 1071.0 24 1236.5 25 1472.0 26 1859.0 27 2502.5 28 3418.0 29 4724.0 30 6482.0 31 8633.5 32 11823.0 33 14729.0 34 17299.0 35 20940.0 36 25159.5 37 29259.0 38 33396.5 39 37852.5 40 41922.5 41 45533.0 42 49594.0 43 55555.0 44 61979.5 45 68010.0 46 72700.0 47 76920.5 48 82749.5 49 86810.0 50 88879.0 51 90087.5 52 89821.0 53 89292.5 54 89236.0 55 86823.0 56 81191.0 57 74493.5 58 68661.0 59 62477.0 60 53704.0 61 45225.0 62 37581.0 63 28908.0 64 21328.5 65 16393.5 66 12614.0 67 9779.0 68 7787.0 69 6156.0 70 4876.5 71 3698.5 72 2749.5 73 2293.0 74 1913.0 75 1455.5 76 970.0 77 553.0 78 347.5 79 221.0 80 124.0 81 56.0 82 32.0 83 22.0 84 11.0 85 5.5 86 5.5 87 3.0 88 2.0 89 3.0 90 1.5 91 0.5 92 1.5 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022563707938638995 2 0.00158611098888392 3 0.03121057107158682 4 0.16664398357402996 5 0.02353584048021301 6 0.06784461842774446 7 0.05244399237438769 8 0.04425761307692229 9 0.03315483615473484 10-11 0.06078386628368056 12-13 0.057304655082257766 14-15 0.05745814969408524 16-17 0.04962992449088396 18-19 0.036429387873721006 20-21 0.022896279597598525 22-23 0.00278848544819915 24-25 0.004451343742996808 26-27 0.003069892236549523 28-29 0.004246684260560174 30-31 0.0018419353419297138 32-33 0.008442203650511189 34-35 0.004656003225433443 36-37 0.005986289861271569 38-39 0.0019698475184526105 40-41 0.0029931449306357847 42-43 0.00319780441307242 44-45 0.004374596437083071 46-47 0.004323431566473911 48-49 0.00199542995375719 50-51 0.0012535393299243885 52-53 7.418906238328013E-4 54-55 0.004425761307692229 56-57 6.39560882614484E-4 58-59 6.139784473099047E-4 60-61 1.790770471320555E-4 62-63 8.442203650511188E-4 64-65 0.0 66-67 0.0037094531191640074 68-69 6.39560882614484E-4 70-71 2.558243530457936E-5 72-73 0.002583825965762515 74-75 0.005372311413961665 76-77 0.0015605285535793408 78-79 7.674730591373808E-5 80-81 0.0 82-83 0.005244399237438768 84-85 0.001355869071142706 86-87 0.0 88-89 0.0011000447180969123 90-91 6.139784473099047E-4 92-93 0.0 94-95 0.00278848544819915 96-97 0.0013047042005335473 98-99 8.186379297465395E-4 100-101 0.0 102-103 0.001432616377056444 104-105 0.0 106-107 4.6048383548242844E-4 108-109 0.0023280016127167217 110-111 7.674730591373808E-5 112-113 3.58154094264111E-4 114-115 0.0 116-117 0.0016116934241884994 118-119 0.002276836742107563 120-121 0.002634990836371674 122-123 9.97714976878595E-4 124-125 0.104606577960425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1954466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.57223240439453 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29613164699538 46.44551451597277 2 11.106485397672179 13.010632878169384 3 3.7253877601834526 6.546128330578559 4 1.7410736303894996 4.0791427724934675 5 0.9866828278469769 2.889610795103917 6 0.6461419981905927 2.2707587570555563 7 0.4452915066683585 1.8257202331397466 8 0.32769989631350205 1.5355291588616349 9 0.2547235187049958 1.3427752622808737 >10 1.3707469548248423 14.126540828094758 >50 0.07165106306748555 2.833450189346413 >100 0.026931640862758677 2.528714818721469 >500 7.891187100457142E-4 0.2888264219471629 >1k 2.63039570015236E-4 0.2766550382342384 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2350 0.120237445931523 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.116487060915872E-5 2 0.0 0.0 0.0 0.0 5.116487060915872E-5 3 0.0 0.0 0.0 5.116487060915872E-5 5.116487060915872E-5 4 0.0 0.0 0.0 5.116487060915872E-5 5.116487060915872E-5 5 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 5.116487060915872E-5 6 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 2.0465948243663488E-4 7 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 2.0465948243663488E-4 8 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 2.0465948243663488E-4 9 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 2.0465948243663488E-4 10-11 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 2.0465948243663488E-4 12-13 0.0 5.116487060915872E-5 0.0 5.116487060915872E-5 3.58154094264111E-4 14-15 0.0 5.116487060915872E-5 0.0 7.674730591373808E-5 3.58154094264111E-4 16-17 0.0 5.116487060915872E-5 0.0 1.0232974121831744E-4 3.58154094264111E-4 18-19 0.0 5.116487060915872E-5 0.0 1.0232974121831744E-4 4.6048383548242844E-4 20-21 0.0 5.116487060915872E-5 0.0 1.0232974121831744E-4 6.39560882614484E-4 22-23 0.0 5.116487060915872E-5 0.0 2.302419177412142E-4 7.418906238328014E-4 24-25 0.0 5.116487060915872E-5 0.0 4.093189648732697E-4 7.930554944419601E-4 26-27 0.0 5.116487060915872E-5 0.0 0.0012279568946198093 8.698028003556982E-4 28-29 0.0 5.116487060915872E-5 0.0 0.005321146543352507 8.698028003556982E-4 30-31 0.0 5.116487060915872E-5 0.0 0.0199542995375719 9.209676709648569E-4 32-33 0.0 5.116487060915872E-5 0.0 0.048043813502000036 9.721325415740156E-4 34-35 0.0 5.116487060915872E-5 0.0 0.08424295945797983 9.721325415740156E-4 36-37 0.0 5.116487060915872E-5 0.0 0.13694277618541328 9.721325415740156E-4 38-39 0.0 5.116487060915872E-5 0.0 0.21629949050021846 9.721325415740156E-4 40-41 0.0 5.116487060915872E-5 0.0 0.309317225267669 9.721325415740156E-4 42-43 0.0 5.116487060915872E-5 0.0 0.40146515723476384 9.721325415740156E-4 44-45 0.0 5.116487060915872E-5 0.0 0.506608966336585 9.97714976878595E-4 46-47 0.0 5.116487060915872E-5 0.0 0.6209368697127502 0.0010232974121831744 48-49 0.0 5.116487060915872E-5 0.0 0.7405859196322678 0.001074462282792333 50-51 0.0 5.116487060915872E-5 0.0 0.8565766813032307 0.0011767920240106505 52-53 0.0 5.116487060915872E-5 0.0 0.9824422630017611 0.0013558690711427059 54-55 0.0 7.674730591373808E-5 0.0 1.1258573953192328 0.0015093636829701823 56-57 0.0 1.0232974121831744E-4 0.0 1.280938118135593 0.0015605285535793408 58-59 0.0 1.0232974121831744E-4 0.0 1.43985620624764 0.0015861109888839202 60-61 0.0 1.0232974121831744E-4 0.0 1.6038396165499937 0.0015861109888839202 62-63 0.0 1.0232974121831744E-4 0.0 1.7731185909603955 0.001637275859493079 64-65 0.0 1.5349461182747614E-4 0.0 1.956672564270752 0.001637275859493079 66-67 0.0 2.0465948243663488E-4 0.0 2.1623041792489612 0.0016628582947976582 68-69 0.0 2.0465948243663488E-4 0.0 2.3884784897767473 0.0016884407301022376 70-71 0.0 2.0465948243663488E-4 0.0 2.623427575614004 0.001790770471320555 72-73 0.0 2.0465948243663488E-4 0.0 2.8501902821537954 0.0020210123890617693 74-75 0.0 2.0465948243663488E-4 0.0 3.09166288899372 0.0020465948243663487 76-77 0.0 2.0465948243663488E-4 0.0 3.3786978131111005 0.0020465948243663487 78-79 0.0 2.0465948243663488E-4 0.0 3.693336184922122 0.0020465948243663487 80-81 0.0 2.0465948243663488E-4 0.0 4.005109323979031 0.0020465948243663487 82-83 0.0 2.0465948243663488E-4 0.0 4.3172917820008125 0.0020465948243663487 84-85 0.0 2.0465948243663488E-4 0.0 4.65006810044278 0.0020465948243663487 86-87 0.0 2.0465948243663488E-4 0.0 5.023111172054157 0.002097759694975507 88-89 0.0 2.0465948243663488E-4 0.0 5.438416426788699 0.002148924565584666 90-91 0.0 2.0465948243663488E-4 0.0 5.867433866846493 0.002148924565584666 92-93 0.0 2.558243530457936E-4 0.0 6.297116450222209 0.002148924565584666 94-95 0.0 2.558243530457936E-4 0.0 6.747495223759328 0.0023024191774121423 96-97 0.0 2.558243530457936E-4 0.0 7.232973098534331 0.00240474891863046 98-99 0.0 2.558243530457936E-4 0.0 7.778953432804664 0.002455913789239618 100-101 0.0 2.558243530457936E-4 0.0 8.355530359699273 0.002455913789239618 102-103 0.0 2.558243530457936E-4 0.0 8.933846892194595 0.0024814962245441976 104-105 0.0 3.069892236549523E-4 0.0 9.5282803589318 0.002507078659848777 106-107 0.0 3.069892236549523E-4 0.0 10.170706474300397 0.002507078659848777 108-109 0.0 3.069892236549523E-4 0.0 10.857211125698784 0.0025326610951533564 110-111 0.0 3.069892236549523E-4 0.0 11.579940505488455 0.0026094084010670946 112-113 0.0 3.069892236549523E-4 0.0 12.31016553882237 0.0026094084010670946 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2295 0.0 56.39838 1 GTATCAA 4080 0.0 52.19124 1 TCAACGC 4900 0.0 42.595257 4 CAACGCA 4965 0.0 41.98055 5 ATCAACG 5240 0.0 39.951397 3 AACGCAG 5345 0.0 39.13244 6 TATCAAC 5475 0.0 38.345524 2 GTACATG 9470 0.0 34.95687 1 TACATGG 9775 0.0 33.497448 2 ACATGGG 9765 0.0 33.104206 3 ATGGGGG 5070 0.0 32.184143 5 CATGGGG 8625 0.0 31.873598 4 ACGCAGA 6710 0.0 31.083006 7 CGCAGAG 7350 0.0 28.375004 8 GCAGAGT 8130 0.0 25.285564 9 AGTACAT 6665 0.0 23.087435 2 GAGTACA 6780 0.0 22.961504 1 TGGGGGG 3715 0.0 21.975525 6 TAACGCT 165 3.1680847E-4 21.684858 4 AGAGTAC 7330 0.0 21.62329 10-11 >>END_MODULE