FastQCFastQC Report
Mon 15 Aug 2016
SRR2921630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813955
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG3900.082.402032
TACGGTC2700.052.8971525
GTACGGT3450.050.025074
AGTACGG5850.042.7269823
GGTATCA22850.041.4182361
GTATCAA33200.039.2632981
GTACATG92300.037.5320241
TACATGG95950.035.788182
ACATGGG97500.034.853013
CATGGGG82350.034.6163064
CTAGTAC5300.033.6919441
ATGGGGG53150.031.9099755
TCAACGC39200.031.7299214
CAACGCA40350.030.5289345
ATCAACG41300.030.4047323
TATCAAC43550.029.2438412
TACGGTA1653.40493E-728.8529955
AACGCAG42600.028.773626
TACGGAC1556.8258796E-626.875175
TATTACG1401.05473286E-425.5053942
Produced by FastQC (version 0.11.5)