##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921630_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1813955
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.12021522033347	31.0	31.0	34.0	26.0	34.0
2	30.213165155695705	31.0	31.0	34.0	26.0	34.0
3	30.216987742253803	31.0	31.0	34.0	26.0	34.0
4	33.68153509872075	37.0	35.0	37.0	28.0	37.0
5	33.81997899617135	37.0	35.0	37.0	30.0	37.0
6	33.68777560634084	37.0	35.0	37.0	29.0	37.0
7	33.69558285624505	37.0	35.0	37.0	29.0	37.0
8	33.68208031621512	37.0	35.0	37.0	29.0	37.0
9	35.30788911522061	39.0	37.0	39.0	29.0	39.0
10-11	35.36691841859363	39.0	37.0	39.0	29.5	39.0
12-13	35.31954238115058	39.0	37.0	39.0	29.0	39.0
14-15	36.62623493967601	40.5	38.0	41.0	29.0	41.0
16-17	36.578721081835	40.5	38.0	41.0	28.5	41.0
18-19	36.5227318208004	40.0	38.0	41.0	28.0	41.0
20-21	36.57600050717906	40.0	38.0	41.0	28.0	41.0
22-23	36.498130604121926	40.0	38.0	41.0	28.0	41.0
24-25	36.481949938118646	40.0	38.0	41.0	28.0	41.0
26-27	36.439470383774676	40.0	38.0	41.0	28.5	41.0
28-29	36.37859979988478	40.0	38.0	41.0	28.0	41.0
30-31	36.28144468853968	40.0	38.0	41.0	27.5	41.0
32-33	36.192538954935486	40.0	37.0	41.0	27.0	41.0
34-35	36.06349606247123	40.0	37.0	41.0	27.0	41.0
36-37	35.95669214506424	40.0	37.0	41.0	26.5	41.0
38-39	35.84225490709527	40.0	37.0	41.0	26.0	41.0
40-41	35.735419015355944	40.0	36.5	41.0	25.5	41.0
42-43	35.4811310093139	40.0	35.5	41.0	24.5	41.0
44-45	35.12769142564176	40.0	35.0	41.0	24.0	41.0
46-47	34.872726170164086	39.0	35.0	41.0	23.0	41.0
48-49	34.67449440587005	39.0	35.0	41.0	22.5	41.0
50-51	34.475676629243836	39.0	35.0	41.0	21.5	41.0
52-53	34.270402518254315	39.0	34.0	40.0	20.5	41.0
54-55	34.02777990633726	38.0	34.0	40.0	19.5	41.0
56-57	33.773989983213475	38.0	34.0	40.0	18.0	41.0
58-59	33.77713256392799	38.0	34.0	40.0	18.5	41.0
60-61	33.561240218197256	37.5	34.0	40.0	18.0	41.0
62-63	33.25119669451557	37.0	34.0	40.0	14.5	41.0
64-65	32.95298119302849	36.5	33.0	39.5	13.0	41.0
66-67	32.634038881890675	36.0	33.0	39.0	11.0	41.0
68-69	32.30121778103646	35.0	33.0	39.0	8.0	40.5
70-71	31.936681725842153	35.0	33.0	38.0	7.0	40.0
72-73	31.59160563520043	35.0	32.0	37.0	6.0	39.5
74-75	31.228440066043536	35.0	32.0	37.0	2.0	39.0
76-77	30.933839317954416	35.0	32.0	36.0	2.0	39.0
78-79	30.66479846523205	35.0	32.0	36.0	2.0	37.5
80-81	30.40810246119667	35.0	31.5	35.0	2.0	37.0
82-83	30.178093172101846	35.0	31.0	35.0	2.0	36.5
84-85	29.96237365315016	35.0	31.0	35.0	2.0	36.0
86-87	29.79220322444603	35.0	31.0	35.0	2.0	36.0
88-89	29.626100978249184	35.0	31.0	35.0	2.0	35.5
90-91	29.4377782249284	34.0	31.0	35.0	2.0	35.0
92-93	29.35353826307709	34.0	31.0	35.0	2.0	35.0
94-95	29.22405682610649	34.0	30.0	35.0	2.0	35.0
96-97	29.132842325195497	34.0	30.0	35.0	2.0	35.0
98-99	29.024688870451584	34.0	30.0	35.0	2.0	35.0
100-101	28.908839800325808	34.0	30.0	35.0	2.0	35.0
102-103	28.020569694397047	33.5	28.0	34.5	2.0	35.0
104-105	28.94542891086052	34.0	30.5	35.0	2.0	35.0
106-107	29.217714607032697	35.0	31.0	35.0	2.0	35.0
108-109	29.215779333004402	35.0	31.0	35.0	2.0	35.0
110-111	29.145847333588762	35.0	31.0	35.0	2.0	35.0
112-113	29.05492390935828	35.0	31.0	35.0	2.0	35.0
114-115	28.928061059949115	35.0	31.0	35.0	2.0	35.0
116-117	28.798264289907962	35.0	31.0	35.0	2.0	35.0
118-119	28.500057333285554	34.0	30.0	35.0	2.0	35.0
120-121	28.364738926820124	34.0	30.0	35.0	2.0	35.0
122-123	28.097397123963937	34.0	30.0	35.0	2.0	35.0
124-125	27.315323423127918	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79241.0
3	13142.0
4	6626.0
5	5794.0
6	6320.0
7	6490.0
8	6561.0
9	6897.0
10	7298.0
11	7517.0
12	7641.0
13	7391.0
14	7652.0
15	6716.0
16	5990.0
17	5543.0
18	5186.0
19	4956.0
20	4863.0
21	5274.0
22	5937.0
23	7886.0
24	10842.0
25	13707.0
26	15447.0
27	17467.0
28	20831.0
29	24907.0
30	30938.0
31	38363.0
32	50256.0
33	69231.0
34	106636.0
35	175911.0
36	350847.0
37	529831.0
38	137764.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22966911898504	17.324907004160963	11.616677382463516	25.828746494390476
2	16.856211078244616	19.52953435865769	37.94782589518314	25.666428667914552
3	19.388224864109084	24.215515630142946	28.221313495474348	28.174946010273622
4	14.373653686370055	15.044198508172851	34.137248318262344	36.44489948719475
5	14.91323787505438	37.21626764341487	33.03009367365134	14.840400807879417
6	34.39133323327388	34.622591951688946	16.39801440856038	14.588060406476789
7	30.10715142689294	31.4918733158471	19.500732270369113	18.900242986890845
8	28.551840383531363	33.97988798960424	18.859256742520596	18.609014884343804
9	28.14410221485744	15.52175843082327	18.14242032386052	38.19171903045877
10-11	26.677420440185834	25.164078241286436	26.42579427437401	21.732707044153717
12-13	26.40753970255921	22.82650077424367	26.580252416944205	24.18570710625292
14-15	24.27100143678042	24.15495060602025	23.640703981048063	27.933343976151264
16-17	24.877757167677764	27.051918977398348	24.286127503141376	23.784196351782512
18-19	24.81956270377012	26.42774552446841	25.57167317140833	23.18101860035314
20-21	25.29664932059137	25.304259425155863	25.496359419724012	23.902731834528755
22-23	25.589055963522085	24.934710189346653	25.07867728951395	24.39755655761731
24-25	24.968621646541873	25.452725119006125	25.101772849397136	24.476880385054862
26-27	24.605635519580478	25.624383999399925	25.445381877627742	24.324598603391856
28-29	24.801798450347786	25.717955796000975	25.316078696542665	24.16416705710858
30-31	25.130227181051968	25.484080066590735	25.29981959954573	24.085873152811565
32-33	24.61054199451799	25.59968633255312	25.285136558111887	24.50463511481701
34-35	24.807728316505568	25.829914051682607	25.27730445427217	24.085053177539656
36-37	24.94016596398545	25.774506868853607	25.161804134709698	24.123523032451246
38-39	24.87321190532972	25.87036804733238	25.21328934980005	24.04313069753785
40-41	24.74023252222639	25.695305433607622	25.329783361644864	24.234678682521125
42-43	24.80731506588715	25.4424343851107	25.19253329510192	24.557717253900226
44-45	24.573970583410734	25.5365394333429	25.430206108004555	24.459283875241812
46-47	24.590287326167985	25.511601483653262	25.40523237844683	24.49287881173192
48-49	24.458596583524	25.752362013886703	25.428079689465417	24.36096171312388
50-51	24.471474439835283	25.65545441135315	25.433111790307528	24.43995935850404
52-53	24.615456300743237	25.63115511856896	25.3671048572069	24.386283723480897
54-55	24.436639464421155	25.355187399935318	25.484632187942253	24.723540947701274
56-57	24.469889281047806	25.590919705944227	25.22945708093151	24.709733932076457
58-59	24.42153918767362	25.70114457054068	25.40642785698768	24.47088838479802
60-61	24.6695663585744	25.551191435909935	25.421692327543138	24.357549877972524
62-63	24.486727679071148	25.531000363383516	25.54547504812488	24.436796909420455
64-65	24.40294966561652	25.314002309819656	25.51151422215348	24.77153380241034
66-67	24.43836140687645	25.628597653661878	25.256180099331566	24.676860840130107
68-69	24.39471571434243	25.538784071709948	25.40760330141029	24.65889691253733
70-71	24.272048946395522	25.423952703830015	25.616249206118518	24.68774914365594
72-73	24.415905951635892	25.6355681031797	25.329067263659095	24.61945868152531
74-75	24.257784793220114	25.5874138847868	25.456995390556663	24.697805931436427
76-77	24.229177877956474	25.23874533536827	25.55699893253818	24.975077854137076
78-79	24.190438168362395	25.400624544729173	25.590811262979873	24.818126023928567
80-81	24.285179438487607	25.549900143471387	25.408275906180076	24.756644511860927
82-83	24.27210511485357	25.508703417677474	25.525935451283527	24.693256016185426
84-85	24.25273952776744	25.490799321837653	25.402583081779213	24.853878068615696
86-87	24.13440790106684	25.602762638544775	25.500646907566793	24.762182552821596
88-89	24.19015411574989	25.35241167892952	25.54851989714037	24.90891430818022
90-91	24.291541842495747	25.28282910158027	25.572095849523947	24.85353320640004
92-93	24.31037277415556	25.345158192221216	25.672398260894497	24.672070772728727
94-95	24.31325932932153	25.105631735255045	25.762454785868133	24.818654149555293
96-97	24.170204155223473	25.17948423885505	25.669016783106507	24.981294822814966
98-99	24.07115974472864	25.249960787491915	25.58213001241293	25.096749455366517
100-101	23.990341661385308	25.3391348790461	25.693479794428125	24.97704366514047
102-103	23.805509789647115	25.239782885366484	25.857078236410835	25.09762908857557
104-105	23.762763790047973	25.334484829823943	25.7726360439535	25.130115336174587
106-107	23.758309269666327	25.25714770846633	25.868331773191805	25.116211248675533
108-109	23.742435979787007	25.288101296531647	25.85823888933304	25.1112238343483
110-111	23.640976420823062	25.61274381440088	25.720245147141764	25.026034617634295
112-113	23.522342211670427	25.669224401768666	25.83665282088219	24.971780565678717
114-115	23.559412120169554	25.696888797731056	25.76790369321565	24.975795388883743
116-117	23.398227795412225	25.553762247824714	25.862361257937224	25.185648698825837
118-119	23.438221695441655	25.425294994199486	25.882757478820594	25.253725831538265
120-121	23.3203505087007	25.66508722749161	25.96950827896259	25.0450539848451
122-123	23.260240261132793	25.665580450984404	25.951945568300655	25.122233719582148
124-125	23.16900553602926	25.536023731290914	25.95076276976771	25.34420796291211
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	757.0
1	604.0
2	391.5
3	289.0
4	225.5
5	198.0
6	192.0
7	221.5
8	269.5
9	292.0
10	299.0
11	286.0
12	296.0
13	332.0
14	382.0
15	413.5
16	436.5
17	518.5
18	608.0
19	648.0
20	689.5
21	807.5
22	943.0
23	1072.5
24	1276.0
25	1620.5
26	2204.5
27	3157.5
28	4380.0
29	5956.5
30	8057.5
31	10396.5
32	13676.0
33	16975.5
34	19808.0
35	22996.0
36	26909.5
37	30812.5
38	33988.0
39	37611.5
40	40685.0
41	43591.5
42	47090.5
43	51862.0
44	57318.0
45	62412.0
46	66344.5
47	69924.0
48	74985.5
49	78378.0
50	79602.0
51	80243.0
52	79729.0
53	78736.0
54	78284.0
55	76568.0
56	72198.5
57	66744.0
58	61677.0
59	55955.5
60	47760.5
61	39928.0
62	33493.0
63	26136.5
64	19762.0
65	15584.0
66	12339.0
67	9861.0
68	7980.5
69	6214.0
70	4873.5
71	3856.5
72	2995.0
73	2458.0
74	1997.5
75	1558.5
76	1187.0
77	797.5
78	532.5
79	412.0
80	290.0
81	194.0
82	156.0
83	111.0
84	81.5
85	73.5
86	66.0
87	54.0
88	44.0
89	31.0
90	29.0
91	29.0
92	24.0
93	26.5
94	22.0
95	15.0
96	9.5
97	7.5
98	4.5
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02387049292843538
2	0.012128195021375944
3	0.010143581290605335
4	0.01135640079274293
5	0.017530754621807046
6	0.09366274246053513
7	0.06064097510687971
8	0.05011149670195788
9	0.028666642777797686
10-11	0.0522614949102927
12-13	0.01375447571742408
14-15	0.028969847653332086
16-17	0.036301892825345725
18-19	0.043578809838171285
20-21	0.03178138377192378
22-23	0.03823137839692826
24-25	0.0332422799904077
26-27	0.04674867899148546
28-29	0.046224961479198766
30-31	0.026985233922561472
32-33	0.031395486657607274
34-35	0.04578393620569419
36-37	0.010364093927357626
38-39	0.021913443277258807
40-41	0.043826886554517615
42-43	0.024118569644781707
44-45	0.017558318701401083
46-47	0.02527626098773123
48-49	0.03120253810044902
50-51	0.029631385563588953
52-53	0.02615831153474039
54-55	0.024283954122345925
56-57	0.026985233922561472
58-59	0.019239727556637293
60-61	0.023649980291683092
62-63	0.024476902679504177
64-65	0.02169293064050652
66-67	0.029410872926836664
68-69	0.023539723973306944
70-71	0.04793393441402901
72-73	0.023153826858990437
74-75	0.009840376415070936
76-77	0.01692434487073825
78-79	0.02613074745514635
80-81	0.019460240193389582
82-83	0.026020491136770206
84-85	0.012679476613256668
86-87	0.01799934397490566
88-89	0.022905750142644114
90-91	0.022271776311981278
92-93	0.008214095719022797
94-95	0.01237627173772227
96-97	0.015766653527788728
98-99	0.006642943182162732
100-101	0.010226273529387443
102-103	0.004107047859511399
104-105	0.004603201292204051
106-107	9.371787061972321E-4
108-109	0.014884602980779567
110-111	0.0013230758205137391
112-113	0.0025083312430572973
114-115	0.01383716795620619
116-117	0.006201917908658153
118-119	0.2791414340488049
120-121	0.0011025631837614494
122-123	6.615379102568696E-4
124-125	0.31475422488429977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1813955.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.73111442717823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01325548128746	52.267961696233336
2	10.02786875553166	12.781745022390274
3	3.1138121718430036	5.953401594854004
4	1.4641543476449745	3.732487530752495
5	0.8478803520065991	2.701817986714436
6	0.5343740798067227	2.043375337624819
7	0.3659419405322764	1.632532138003631
8	0.27253009750606344	1.3894917463207175
9	0.20473417877377056	1.1743143637126985
>10	1.0877157711678183	12.16324885193274
>50	0.04922720962957651	2.109973032984855
>100	0.01824628701994998	1.9169371648513531
>500	2.593272500677508E-4	0.1327135336246709
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1025631837614494E-4
7	0.0	0.0	0.0	0.0	1.1025631837614494E-4
8	0.0	0.0	0.0	0.0	1.1025631837614494E-4
9	0.0	0.0	0.0	0.0	2.2051263675228988E-4
10-11	0.0	0.0	0.0	8.269223878210871E-5	2.2051263675228988E-4
12-13	0.0	0.0	0.0	1.1025631837614494E-4	5.512815918807247E-4
14-15	0.0	0.0	0.0	1.3782039797018117E-4	8.544864674151233E-4
16-17	0.0	0.0	0.0	1.9294855715825364E-4	9.923068653853045E-4
18-19	0.0	0.0	0.0	2.7564079594036235E-4	0.001047435024573377
20-21	0.0	0.0	0.0	4.6858935309861604E-4	0.0011025631837614494
22-23	0.0	0.0	0.0	6.339738306628334E-4	0.0011301272633554857
24-25	0.0	0.0	0.0	0.0011025631837614494	0.0012403835817316306
26-27	0.0	0.0	0.0	0.0019019214919885003	0.0012955117409197033
28-29	0.0	0.0	0.0	0.005650636316777429	0.0013230758205137394
30-31	0.0	0.0	0.0	0.01896408676069693	0.0013230758205137394
32-33	0.0	0.0	0.0	0.044571116703556596	0.0013782039797018118
34-35	0.0	0.0	0.0	0.07941211331041839	0.0013782039797018118
36-37	0.0	0.0	0.0	0.13329988891675923	0.0013782039797018118
38-39	0.0	0.0	0.0	0.20951456899426943	0.0013782039797018118
40-41	0.0	0.0	0.0	0.2915728339457153	0.0013782039797018118
42-43	0.0	0.0	0.0	0.37718686516479183	0.0013782039797018118
44-45	0.0	0.0	0.0	0.47611434682778786	0.0014608962184839203
46-47	0.0	0.0	0.0	0.5862328448059626	0.0016262806960481379
48-49	0.0	0.0	0.0	0.6936224989043278	0.0017089729348302466
50-51	0.0	0.0	0.0	0.7959954905165785	0.001764101094018319
52-53	0.0	0.0	0.0	0.9059486040171889	0.0017916651736123554
54-55	0.0	0.0	0.0	1.0357754189050996	0.0018192292532063915
56-57	0.0	0.0	0.0	1.178998376475712	0.0018192292532063915
58-59	0.0	0.0	0.0	1.3269347916569045	0.0018467933328004276
60-61	0.0	0.0	0.0	1.470350697784675	0.0019294855715825366
62-63	0.0	0.0	0.0	1.619499932468005	0.001984613730770609
64-65	0.0	5.512815918807247E-5	0.0	1.7775248007806148	0.002012177810364645
66-67	0.0	5.512815918807247E-5	0.0	1.9597784950563821	0.002067305969552718
68-69	0.0	5.512815918807247E-5	0.0	2.158956534202888	0.002094870049146754
70-71	0.0	5.512815918807247E-5	0.0	2.3668172584215155	0.00212243412874079
72-73	0.0	5.512815918807247E-5	0.0	2.5680074753783857	0.0022602545267109714
74-75	0.0	5.512815918807247E-5	0.0	2.7851848584997976	0.0022602545267109714
76-77	0.0	5.512815918807247E-5	0.0	3.0408968248936716	0.0022602545267109714
78-79	0.0	5.512815918807247E-5	0.0	3.318136337450488	0.0022602545267109714
80-81	0.0	5.512815918807247E-5	0.0	3.5898630340884976	0.0023429467654930798
82-83	0.0	5.512815918807247E-5	0.0	3.8699967750026874	0.002535895322651334
84-85	0.0	5.512815918807247E-5	0.0	4.162065762381095	0.002535895322651334
86-87	0.0	5.512815918807247E-5	0.0	4.491897538803332	0.0026185875614334426
88-89	0.0	5.512815918807247E-5	0.0	4.863323511332972	0.002701279800215551
90-91	0.0	5.512815918807247E-5	0.0	5.250764214106745	0.002701279800215551
92-93	0.0	5.512815918807247E-5	0.0	5.6322786397678	0.0027564079594036236
94-95	0.0	5.512815918807247E-5	0.0	6.027382156668716	0.0027564079594036236
96-97	0.0	5.512815918807247E-5	0.0	6.45969718102158	0.0028115361185916963
98-99	0.0	5.512815918807247E-5	0.0	6.940662805857918	0.0028942283573738046
100-101	0.0	5.512815918807247E-5	0.0	7.460328398444283	0.002921792436967841
102-103	0.0	5.512815918807247E-5	0.0	7.9753632256588505	0.0029493565165618772
104-105	0.0	5.512815918807247E-5	0.0	8.517631363512326	0.0029769205961559134
106-107	0.0	5.512815918807247E-5	0.0	9.08790460623334	0.0029769205961559134
108-109	0.0	5.512815918807247E-5	0.0	9.70669062903986	0.0029769205961559134
110-111	0.0	5.512815918807247E-5	0.0	10.356982394822364	0.00300448467574995
112-113	0.0	5.512815918807247E-5	0.0	11.01146390070316	0.003032048755343986
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	390	0.0	82.40203	2
TACGGTC	270	0.0	52.897152	5
GTACGGT	345	0.0	50.02507	4
AGTACGG	585	0.0	42.726982	3
GGTATCA	2285	0.0	41.418236	1
GTATCAA	3320	0.0	39.263298	1
GTACATG	9230	0.0	37.532024	1
TACATGG	9595	0.0	35.78818	2
ACATGGG	9750	0.0	34.85301	3
CATGGGG	8235	0.0	34.616306	4
CTAGTAC	530	0.0	33.691944	1
ATGGGGG	5315	0.0	31.909975	5
TCAACGC	3920	0.0	31.729921	4
CAACGCA	4035	0.0	30.528934	5
ATCAACG	4130	0.0	30.404732	3
TATCAAC	4355	0.0	29.243841	2
TACGGTA	165	3.40493E-7	28.852995	5
AACGCAG	4260	0.0	28.77362	6
TACGGAC	155	6.8258796E-6	26.87517	5
TATTACG	140	1.05473286E-4	25.505394	2
>>END_MODULE