FastQCFastQC Report
Mon 15 Aug 2016
SRR2921630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813955
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.13186655677786935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.11687169747871363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.054.1889651
GTATCAA45200.046.205511
GTACATG94300.035.87861
TCAACGC58650.035.194614
TATCAAC60150.034.6183132
ATCAACG59900.034.462073
CAACGCA60000.034.3542945
TACATGG98700.034.0335582
ACATGGG97550.033.7601133
CATGGGG82650.033.2034384
AACGCAG62950.032.9481936
ATGGGGG49500.031.6524035
ACGCAGA75400.027.4280037
CGCAGAG79700.026.0963158
TAGGCGG1901.1562624E-625.0837555
TATACTA5750.023.8295675
GCAGAGT87000.023.7011229
AGAGTAC79150.022.55059210-11
TGGGGGG34550.022.4255626
TACCGTA1602.5829687E-422.3494747
Produced by FastQC (version 0.11.5)