##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1813955
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76198748039505	33.0	31.0	34.0	30.0	34.0
2	31.932449261420487	33.0	31.0	34.0	30.0	34.0
3	32.001264088690185	34.0	31.0	34.0	30.0	34.0
4	35.39563936260822	37.0	35.0	37.0	33.0	37.0
5	35.25415183948885	37.0	35.0	37.0	33.0	37.0
6	35.286718799529204	37.0	35.0	37.0	33.0	37.0
7	35.175803699650764	37.0	35.0	37.0	33.0	37.0
8	35.208302300773724	37.0	36.0	37.0	33.0	37.0
9	36.93412681130458	39.0	38.0	39.0	34.0	39.0
10-11	36.89237357045792	39.0	38.0	39.0	34.0	39.0
12-13	36.82176625109223	39.0	38.0	39.0	33.0	39.0
14-15	38.26077410961132	41.0	39.0	41.0	34.0	41.0
16-17	38.26626101529531	41.0	39.0	41.0	34.0	41.0
18-19	38.278627639605176	41.0	39.0	41.0	34.0	41.0
20-21	38.212519329310815	41.0	39.0	41.0	34.0	41.0
22-23	38.21646292217834	41.0	39.0	41.0	34.0	41.0
24-25	38.179856446273476	41.0	39.0	41.0	34.0	41.0
26-27	38.10599353346693	41.0	39.0	41.0	34.0	41.0
28-29	38.036873571836125	41.0	39.0	41.0	33.0	41.0
30-31	37.92384926858715	41.0	38.0	41.0	33.0	41.0
32-33	37.809992805775224	41.0	38.0	41.0	33.0	41.0
34-35	37.67649087215504	41.0	38.0	41.0	33.0	41.0
36-37	37.59858486015364	41.0	38.0	41.0	33.0	41.0
38-39	37.51351411694336	40.0	38.0	41.0	32.5	41.0
40-41	37.39669093224474	40.0	38.0	41.0	32.0	41.0
42-43	37.23813545539994	40.0	38.0	41.0	32.0	41.0
44-45	37.08607187058114	40.0	37.0	41.0	31.0	41.0
46-47	36.89629566334335	40.0	37.0	41.0	31.0	41.0
48-49	36.71314641212158	40.0	37.0	41.0	31.0	41.0
50-51	36.47117100479339	40.0	36.0	41.0	30.0	41.0
52-53	36.34076369038924	40.0	36.0	41.0	30.0	41.0
54-55	36.207434307907306	40.0	35.0	41.0	30.0	41.0
56-57	36.00863279408806	39.0	35.0	41.0	29.5	41.0
58-59	35.81192890672591	39.0	35.0	41.0	29.5	41.0
60-61	35.56024763569107	39.0	35.0	41.0	29.0	41.0
62-63	35.209802613626024	37.5	35.0	41.0	28.5	41.0
64-65	34.924885402339086	37.0	35.0	40.0	28.5	41.0
66-67	34.62174006521661	37.0	35.0	39.5	28.5	41.0
68-69	34.25441810849773	36.0	35.0	39.0	28.0	41.0
70-71	33.881910245844026	35.5	35.0	39.0	28.0	41.0
72-73	33.56286181299977	35.0	34.0	37.5	27.5	40.0
74-75	33.244682475585115	35.0	34.0	37.0	27.0	39.0
76-77	32.92715006711853	35.0	34.0	36.5	27.0	39.0
78-79	32.667246155499996	35.0	34.0	36.0	26.0	38.0
80-81	32.41629643513758	35.0	34.0	36.0	26.0	37.0
82-83	32.19605447764691	35.0	34.0	35.0	26.0	37.0
84-85	31.984926307433206	35.0	34.0	35.0	25.5	36.5
86-87	31.85795127222009	35.0	34.0	35.0	25.0	36.0
88-89	31.759204610919234	35.0	34.0	35.0	25.0	36.0
90-91	31.651743565854723	35.0	34.0	35.0	25.0	36.0
92-93	31.575821065020907	35.0	33.5	35.0	25.0	35.0
94-95	31.510932465248587	35.0	33.0	35.0	25.0	35.0
96-97	31.448022415109527	35.0	33.0	35.0	24.0	35.0
98-99	31.372147875774203	35.0	33.0	35.0	24.0	35.0
100-101	31.303326157484612	35.0	33.0	35.0	24.0	35.0
102-103	30.61138175974597	34.0	31.5	35.0	21.5	35.0
104-105	31.127486624530377	35.0	33.0	35.0	23.0	35.0
106-107	31.229961051955534	35.0	33.0	35.0	23.5	35.0
108-109	31.213532309235895	35.0	33.0	35.0	23.5	35.0
110-111	31.14078767113848	35.0	33.0	35.0	23.0	35.0
112-113	31.097670283992713	35.0	33.0	35.0	23.0	35.0
114-115	30.967623232108842	35.0	33.0	35.0	20.0	35.0
116-117	31.146073083400637	35.0	33.0	35.0	23.0	35.0
118-119	31.119894650087794	35.0	34.0	35.0	21.0	35.0
120-121	31.03045307077629	35.0	33.5	35.0	19.0	35.0
122-123	30.886116524390076	35.0	33.0	35.0	13.5	35.0
124-125	30.234486522543282	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28453.0
3	7596.0
4	4172.0
5	3327.0
6	3599.0
7	3917.0
8	4239.0
9	4669.0
10	4977.0
11	5513.0
12	5859.0
13	7059.0
14	9464.0
15	8537.0
16	5136.0
17	4508.0
18	4116.0
19	3857.0
20	3684.0
21	3738.0
22	4192.0
23	4992.0
24	6727.0
25	8692.0
26	10499.0
27	12460.0
28	15545.0
29	18461.0
30	22729.0
31	28402.0
32	37024.0
33	50344.0
34	78010.0
35	138928.0
36	322136.0
37	676373.0
38	251660.0
39	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.85921379850347	17.816247870175843	11.109824485947296	27.21471384537339
2	16.277262044750522	19.850566975555193	36.65563532740916	27.216535652285128
3	18.615502376301617	23.44360240486797	28.659168090388796	29.28172712844161
4	13.494047093125994	15.062897596748542	33.98033000530129	37.462725304824176
5	14.7623091387325	37.205278220447866	32.82014237740489	15.212270263414743
6	35.01787428420112	33.77075678836627	16.316738936149086	14.894629991283528
7	30.448732798962148	31.486531507381248	19.567428509651556	18.49730718400505
8	28.41695332359334	33.88932919245752	18.748052415060478	18.945665068888665
9	28.169017968127008	14.622885510681499	18.392320834358156	38.815775686833334
10-11	26.932338035658827	24.696365990343068	26.454731957517442	21.916564016480663
12-13	26.777055708148424	22.3328754168341	26.826919974196343	24.063148900821133
14-15	24.306595957344935	23.608723856342547	24.078283691918	28.00639649439452
16-17	24.418958359705144	26.599993215722645	24.927641269476187	24.053407155096025
18-19	24.479807064184083	25.785283901159552	26.56206785016149	23.172841184494875
20-21	25.14013643778979	24.780914038898803	26.401123131755412	23.677826391555996
22-23	25.056659714791174	24.385288265774445	26.185609011306582	24.372443008127796
24-25	24.50276013127783	24.846446506709714	26.23988140228893	24.41091195972353
26-27	24.271449383025733	25.050326852335512	26.43111430252895	24.2471094621098
28-29	24.321124494759392	25.324562735839823	26.318242800519442	24.03606996888134
30-31	24.749981049251275	24.943623245332947	26.240957047266612	24.065438658149166
32-33	24.43428336402884	24.964749100430122	26.16367940275121	24.437288132789828
34-35	24.55270926998426	25.08916043917271	26.31121941269058	24.046910878152453
36-37	24.5553346609065	25.122282279103516	26.241925951409055	24.08045710858093
38-39	24.556187002508114	25.233948844803066	26.24725627463082	23.962607878057998
40-41	24.48361798329506	25.041037392196735	26.267293935782927	24.208050688725283
42-43	24.43126828777498	24.904782972436195	26.084407341710392	24.57954139807843
44-45	24.379570695724322	24.98796768226963	26.151560342142332	24.48090127986372
46-47	24.442111188263542	25.073220537640662	25.945471821429273	24.539196452666523
48-49	24.33131606040517	25.335606498090456	25.917330859865455	24.41574658163891
50-51	24.392120304664793	25.321815419355126	25.892069586003412	24.39399468997667
52-53	24.57728732884623	25.279323064336783	25.691823153920833	24.451566452896156
54-55	24.276334106088964	25.05586168684928	25.904827012082006	24.76297719497975
56-57	24.315000148847098	25.23286301326062	25.66509844305937	24.787038394832912
58-59	24.296339133224766	25.244770780984176	25.894874257280552	24.564015828510506
60-61	24.517593487150297	25.17067012504462	25.877772433401645	24.43396395440344
62-63	24.365565066482464	25.137091310227422	26.00456906717267	24.49277455611744
64-65	24.180588823868288	24.925811279772653	26.02412959527662	24.86947030108244
66-67	24.2361494151643	25.25994612118571	25.70537114939173	24.798533314258258
68-69	24.21911328745733	25.298334157962127	25.719542599772044	24.763009954808503
70-71	24.110079908266744	25.051861815756176	25.964480927035126	24.873577348941954
72-73	24.207205789241126	25.30039282403573	25.685057719029516	24.807343667693633
74-75	24.080843487376715	25.332793413790167	25.725879834087763	24.86048326474536
76-77	24.05844344679175	25.00371432217824	25.78974483226838	25.14809739876164
78-79	23.97820448892928	25.12671920941657	25.840988582109283	25.05408771954487
80-81	24.118349132144953	25.34373785457743	25.552122296308344	24.985790716969277
82-83	24.05407826711378	25.29210616629522	25.690815543160205	24.963000023430794
84-85	24.006256053867777	25.299199580136534	25.553537829334545	25.14100653666115
86-87	23.86277498614905	25.44305123335474	25.589140855203134	25.105032925293074
88-89	23.87108031129001	25.14022687135237	25.703367071210636	25.28532574614698
90-91	23.870236132266704	25.05533531685981	25.796015416157413	25.27841313471607
92-93	23.886783299475457	25.169257230747178	25.849731663685148	25.094227806092213
94-95	23.815265195525107	24.955268971923715	25.915990291623427	25.313475540927755
96-97	23.68535227713133	25.043262490717655	25.813993617163206	25.457391614987813
98-99	23.61326227264646	25.06912429693885	25.73571102722385	25.58190240319084
100-101	23.494877215807445	25.117684837826737	25.862493832537194	25.524944113828624
102-103	23.453502465228112	24.99940047284087	25.955722093940253	25.59137496799076
104-105	23.463867626264157	25.062887447593795	25.886447017704405	25.586797908437642
106-107	23.430368616468524	25.01128755281996	25.870464650251247	25.687879180460264
108-109	23.427388033247535	25.03331203494773	25.83459258430274	25.704707347501998
110-111	23.299956889755748	25.32018452507616	25.675458142819497	25.70440044234859
112-113	23.117382994358724	25.36140722654581	25.800338597900385	25.720871181195083
114-115	23.126152523078026	25.362343608303405	25.74655931376467	25.764944554853898
116-117	22.94891952355173	25.227380229138852	25.86222009546166	25.961480151847766
118-119	23.042704650858298	25.116619462074386	25.798515639536085	26.042160247531232
120-121	22.935398713327363	25.274249705124884	25.809642023404223	25.980709558143527
122-123	22.77779829795257	25.432719951618942	25.698440901620952	26.091040848807534
124-125	22.58456802404691	25.32491776163748	25.768664127793212	26.321850086522403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42.0
1	35.5
2	37.0
3	69.0
4	72.0
5	76.0
6	191.0
7	445.0
8	727.0
9	835.0
10	685.5
11	501.5
12	463.0
13	492.0
14	549.5
15	626.0
16	662.0
17	685.0
18	748.0
19	822.0
20	894.5
21	963.5
22	1065.0
23	1217.5
24	1409.5
25	1758.0
26	2366.5
27	3316.5
28	4468.5
29	6016.5
30	8037.5
31	10277.5
32	13324.0
33	16468.0
34	19470.5
35	22585.0
36	26354.5
37	30347.0
38	33409.0
39	36897.0
40	40347.0
41	43350.5
42	46648.5
43	51446.5
44	56872.0
45	62068.5
46	66266.5
47	70065.5
48	75352.5
49	79184.5
50	80565.0
51	81151.5
52	80743.5
53	79685.0
54	79694.0
55	78130.5
56	73435.5
57	67546.5
58	62121.0
59	56357.0
60	48104.5
61	40067.5
62	33246.0
63	25764.0
64	19224.0
65	14870.0
66	11566.0
67	9191.5
68	7436.0
69	5678.5
70	4343.5
71	3445.5
72	2720.5
73	2238.0
74	1761.5
75	1351.0
76	985.0
77	609.0
78	377.5
79	213.0
80	130.0
81	81.0
82	49.0
83	23.0
84	13.5
85	12.5
86	11.0
87	8.5
88	4.0
89	2.0
90	2.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0234294676549308
2	0.001764101094018319
3	0.03489612476604987
4	0.16951908950332287
5	0.025634594022453702
6	0.07139096614855385
7	0.054190980481875234
8	0.044764065260714844
9	0.03489612476604987
10-11	0.06416917729491635
12-13	0.056974952520872896
14-15	0.05992430903743477
16-17	0.05179290555719409
18-19	0.03911342894393742
20-21	0.02505574835097894
22-23	0.0030871769145320582
24-25	0.005071790645302668
26-27	0.0034455099492545294
28-29	0.005044226565708631
30-31	0.00234294676549308
32-33	0.009454479300754428
34-35	0.004961534326926522
36-37	0.006560250943380625
38-39	0.0023980749246811524
40-41	0.003335253630878384
42-43	0.003996791541135254
44-45	0.004961534326926522
46-47	0.0047134576105801965
48-49	0.002067305969552718
50-51	0.0014884602980779567
52-53	8.820505470091595E-4
54-55	0.005512815918807247
56-57	7.166660694449421E-4
58-59	7.166660694449421E-4
60-61	1.3782039797018117E-4
62-63	9.923068653853045E-4
64-65	0.0
66-67	0.0038314070635710367
68-69	4.1346119391054355E-4
70-71	0.0
72-73	0.0021224341287407905
74-75	0.005760892635153573
76-77	0.0016814088552362103
78-79	1.9294855715825364E-4
80-81	0.0
82-83	0.005650636316777429
84-85	0.001764101094018319
86-87	0.0
88-89	0.0010749991041674133
90-91	7.717942286330146E-4
92-93	0.0
94-95	0.003169869153314167
96-97	0.0015435884572660291
98-99	6.891019898509059E-4
100-101	0.0
102-103	0.0014057680592958481
104-105	0.0
106-107	5.512815918807247E-4
108-109	0.002535895322651334
110-111	5.512815918807247E-5
112-113	2.2051263675228988E-4
114-115	0.0
116-117	0.0018192292532063915
118-119	0.00234294676549308
120-121	0.0028391001981857324
122-123	0.0012128195021375943
124-125	0.11006336981898669
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1813955.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.3844977605037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4744063125134	47.99022109995326
2	11.317660440194162	13.668224830100915
3	3.692294402935721	6.6887202931557725
4	1.6866832338854987	4.073980798369521
5	0.959279911248067	2.896281782622754
6	0.6459320717622223	2.340257024445806
7	0.3951222355817386	1.6701480424727497
8	0.3053594516387476	1.4751181698902878
9	0.22206569371766566	1.2068392846481175
>10	1.2196058275594257	12.98911812987493
>50	0.059237288225043004	2.4130949196162934
>100	0.021803630804797507	2.1446937574096316
>500	2.7474996674309543E-4	0.1184018585565163
>1k	2.7474996674309543E-4	0.3249000088834812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2392	0.13186655677786935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2120	0.11687169747871363	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.1025631837614494E-4	0.0
12-13	0.0	0.0	0.0	1.6538447756421742E-4	1.6538447756421742E-4
14-15	0.0	0.0	0.0	2.2051263675228988E-4	3.032048755343986E-4
16-17	0.0	0.0	0.0	2.4807671634632613E-4	3.858971143165073E-4
18-19	0.0	0.0	0.0	3.3076895512843484E-4	3.858971143165073E-4
20-21	0.0	0.0	0.0	4.961534326926523E-4	4.134611939105435E-4
22-23	0.0	0.0	0.0	6.339738306628334E-4	4.6858935309861604E-4
24-25	0.0	0.0	0.0	0.0011025631837614494	4.961534326926523E-4
26-27	0.0	0.0	0.0	0.0019019214919885003	4.961534326926523E-4
28-29	0.0	0.0	0.0	0.005760892635153574	4.961534326926523E-4
30-31	0.0	0.0	0.0	0.019349983875013438	4.961534326926523E-4
32-33	0.0	0.0	0.0	0.04523265461381346	5.237175122866885E-4
34-35	0.0	0.0	0.0	0.08065249689215002	6.615379102568697E-4
36-37	0.0	0.0	0.0	0.13514668224955967	6.615379102568697E-4
38-39	0.0	0.0	0.0	0.21171969536179233	7.166660694449421E-4
40-41	0.0	0.0	0.0	0.2949080875765937	7.166660694449421E-4
42-43	0.0	0.0	0.0	0.38167981013861974	7.166660694449421E-4
44-45	0.0	0.0	0.0	0.4813790860302488	7.166660694449421E-4
46-47	0.0	0.0	0.0	0.5919110452023342	7.166660694449421E-4
48-49	0.0	0.0	0.0	0.6996314682558278	7.166660694449421E-4
50-51	0.0	0.0	0.0	0.8037961250416907	7.166660694449421E-4
52-53	0.0	0.0	0.0	0.9147966735668747	7.993583082270509E-4
54-55	0.0	0.0	0.0	1.0456709234793586	8.544864674151233E-4
56-57	0.0	0.0	0.0	1.1902169568704846	8.820505470091595E-4
58-59	0.0	0.0	0.0	1.3405238828967643	8.820505470091595E-4
60-61	0.0	0.0	0.0	1.486282735790028	8.820505470091595E-4
62-63	0.0	0.0	0.0	1.6376646609204748	9.37178706197232E-4
64-65	0.0	0.0	0.0	1.7982805527149242	9.647427857912682E-4
66-67	0.0	0.0	0.0	1.9828771937561847	9.923068653853045E-4
68-69	0.0	0.0	0.0	2.1855283069315394	0.001074999104167413
70-71	0.0	0.0	0.0	2.3971101818953615	0.001185255422543558
72-73	0.0	0.0	0.0	2.601608088403516	0.0014057680592958481
74-75	0.0	0.0	0.0	2.8220655969966177	0.0014608962184839203
76-77	0.0	0.0	0.0	3.081360893737717	0.0015435884572660291
78-79	0.0	0.0	0.0	3.3650228368399437	0.0015435884572660291
80-81	0.0	0.0	0.0	3.643144399943769	0.001653844775642174
82-83	0.0	0.0	0.0	3.9297005714033704	0.0018192292532063915
84-85	0.0	0.0	0.0	4.227199682461803	0.0018192292532063915
86-87	0.0	0.0	0.0	4.565741708035755	0.0018192292532063915
88-89	0.0	0.0	0.0	4.947476646333564	0.0018743574123944642
90-91	0.0	0.0	0.0	5.345694904228605	0.0019294855715825366
92-93	0.0	0.0	0.0	5.737849064612959	0.0019294855715825366
94-95	0.0	0.0	0.0	6.140918600516551	0.0019294855715825366
96-97	0.0	0.0	0.0	6.58153041282722	0.001957049651176573
98-99	0.0	0.0	0.0	7.074155643331836	0.0020397418899586813
100-101	0.0	0.0	0.0	7.605866738700795	0.0020397418899586813
102-103	0.0	0.0	0.0	8.129391302430324	0.0020397418899586813
104-105	0.0	0.0	0.0	8.677916486351645	0.002067305969552718
106-107	0.0	0.0	0.0	9.255246133448736	0.00212243412874079
108-109	0.0	0.0	0.0	9.88635329983379	0.0022051263675228992
110-111	0.0	0.0	0.0	10.55064761805006	0.002453203083869225
112-113	0.0	0.0	0.0	11.22268744263226	0.0026185875614334426
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2830	0.0	54.188965	1
GTATCAA	4520	0.0	46.20551	1
GTACATG	9430	0.0	35.8786	1
TCAACGC	5865	0.0	35.19461	4
TATCAAC	6015	0.0	34.618313	2
ATCAACG	5990	0.0	34.46207	3
CAACGCA	6000	0.0	34.354294	5
TACATGG	9870	0.0	34.033558	2
ACATGGG	9755	0.0	33.760113	3
CATGGGG	8265	0.0	33.203438	4
AACGCAG	6295	0.0	32.948193	6
ATGGGGG	4950	0.0	31.652403	5
ACGCAGA	7540	0.0	27.428003	7
CGCAGAG	7970	0.0	26.096315	8
TAGGCGG	190	1.1562624E-6	25.083755	5
TATACTA	575	0.0	23.829567	5
GCAGAGT	8700	0.0	23.701122	9
AGAGTAC	7915	0.0	22.550592	10-11
TGGGGGG	3455	0.0	22.425562	6
TACCGTA	160	2.5829687E-4	22.349474	7
>>END_MODULE