FastQCFastQC Report
Mon 15 Aug 2016
SRR2921629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685389
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31950.040.9886441
GGTATCA22550.040.38841
GTACATG86700.035.6336251
TCAACGC37400.033.8935134
TACATGG91650.033.1176222
CAACGCA39000.032.502045
ACATGGG93950.031.7358023
TATCAAC42900.030.3814332
AACGCAG42300.029.8230766
ATCAACG42800.029.4781783
CATGGGG76650.029.3486544
ATGGGGG43400.026.327355
ACGCAGA50600.024.9244887
CGCAGAG52950.023.7052488
GAGTACA63000.021.448511
ATGGGTA8400.021.253855
GCAGAGT60750.021.0539259
AGTACAT63700.021.021542
ATGGGGA31500.020.9704675
CGCCTTA6850.020.8517342
Produced by FastQC (version 0.11.5)