##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921629_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685389
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.214014687410444	31.0	31.0	34.0	27.0	34.0
2	30.482167024942015	31.0	31.0	34.0	27.0	34.0
3	30.53561996666645	33.0	31.0	34.0	27.0	34.0
4	34.046277150260266	37.0	35.0	37.0	30.0	37.0
5	34.02344087922729	37.0	35.0	37.0	30.0	37.0
6	34.071141439750704	37.0	35.0	37.0	30.0	37.0
7	34.00494900583782	37.0	35.0	37.0	30.0	37.0
8	34.02880462611302	37.0	35.0	37.0	30.0	37.0
9	35.64620452607677	39.0	37.0	39.0	30.0	39.0
10-11	35.66864563611131	39.0	37.0	39.0	30.0	39.0
12-13	35.608731574728445	39.0	37.0	39.0	30.0	39.0
14-15	36.929053174074355	41.0	38.0	41.0	30.0	41.0
16-17	36.89409358907646	41.0	38.0	41.0	30.0	41.0
18-19	36.84809649285714	41.0	38.0	41.0	30.0	41.0
20-21	36.89764440138153	41.0	38.0	41.0	30.0	41.0
22-23	36.827259166874825	40.0	38.0	41.0	30.0	41.0
24-25	36.810366330858926	40.5	38.0	41.0	30.0	41.0
26-27	36.76174135466648	40.0	38.0	41.0	30.0	41.0
28-29	36.70139326885366	40.0	38.0	41.0	30.0	41.0
30-31	36.60810916648916	40.0	38.0	41.0	30.0	41.0
32-33	36.51101882117422	40.0	38.0	41.0	29.5	41.0
34-35	36.37378314442542	40.0	37.0	41.0	28.5	41.0
36-37	36.267420755683105	40.0	37.0	41.0	28.0	41.0
38-39	36.14776054667498	40.0	37.0	41.0	27.0	41.0
40-41	36.02890549303454	40.0	37.0	41.0	27.0	41.0
42-43	35.79330291107868	40.0	36.0	41.0	26.5	41.0
44-45	35.47786950075027	40.0	35.0	41.0	26.0	41.0
46-47	35.23943374496926	39.5	35.0	41.0	25.0	41.0
48-49	35.04466149951139	39.0	35.0	41.0	24.0	41.0
50-51	34.8377914534864	39.0	35.0	41.0	24.0	41.0
52-53	34.62025918052153	39.0	35.0	40.5	23.0	41.0
54-55	34.366492246003745	38.0	34.0	40.0	23.0	41.0
56-57	34.10202511111678	38.0	34.0	40.0	22.0	41.0
58-59	34.0896045364008	38.0	34.0	40.0	22.5	41.0
60-61	33.862598189498094	37.0	34.0	40.0	22.0	41.0
62-63	33.55026643700653	37.0	34.0	40.0	21.5	41.0
64-65	33.24452752450621	36.0	34.0	39.5	20.5	41.0
66-67	32.914817884773186	36.0	33.0	39.0	20.0	41.0
68-69	32.57449556155879	35.0	33.0	39.0	18.5	40.5
70-71	32.21390877714285	35.0	33.0	37.5	17.5	40.0
72-73	31.87491404061614	35.0	33.0	37.0	16.0	39.0
74-75	31.511629362716857	35.0	32.5	36.5	12.5	39.0
76-77	31.228333933590406	35.0	32.0	36.0	11.0	38.5
78-79	30.966509808714783	35.0	32.0	36.0	8.0	37.0
80-81	30.70844772334458	35.0	32.0	35.0	7.0	37.0
82-83	30.49554494541023	35.0	32.0	35.0	6.5	36.5
84-85	30.284554188973583	35.0	31.5	35.0	2.0	36.0
86-87	30.116580801227492	35.0	31.0	35.0	2.0	36.0
88-89	29.95295744780582	35.0	31.0	35.0	2.0	35.5
90-91	29.760949252665114	35.0	31.0	35.0	2.0	35.0
92-93	29.682527001184887	34.5	31.0	35.0	2.0	35.0
94-95	29.55255611612512	34.0	31.0	35.0	2.0	35.0
96-97	29.456244819445242	34.0	31.0	35.0	2.0	35.0
98-99	29.346299281649518	34.0	30.5	35.0	2.0	35.0
100-101	29.222410671957633	34.0	30.0	35.0	2.0	35.0
102-103	28.348331453450804	33.5	29.0	34.5	2.0	35.0
104-105	29.268922189476733	34.0	30.5	35.0	2.0	35.0
106-107	29.53824072662157	35.0	31.0	35.0	2.0	35.0
108-109	29.525665588181717	35.0	31.0	35.0	2.0	35.0
110-111	29.44639130788204	35.0	31.0	35.0	2.0	35.0
112-113	29.348802561307807	35.0	31.0	35.0	2.0	35.0
114-115	29.218766112749044	35.0	31.0	35.0	2.0	35.0
116-117	29.086533435307814	35.0	31.0	35.0	2.0	35.0
118-119	28.773107573385136	35.0	31.0	35.0	2.0	35.0
120-121	28.625790247830025	35.0	30.0	35.0	2.0	35.0
122-123	28.35557844509487	34.0	30.0	35.0	2.0	35.0
124-125	27.56450943966052	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61401.0
3	11394.0
4	5973.0
5	5195.0
6	5832.0
7	5983.0
8	5941.0
9	6138.0
10	6548.0
11	6884.0
12	6662.0
13	6544.0
14	6454.0
15	6176.0
16	5463.0
17	5128.0
18	4721.0
19	4521.0
20	4561.0
21	4866.0
22	5467.0
23	7120.0
24	10078.0
25	12864.0
26	14751.0
27	16385.0
28	19454.0
29	23469.0
30	28682.0
31	35709.0
32	47122.0
33	64198.0
34	98396.0
35	164953.0
36	332276.0
37	500694.0
38	127333.0
39	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.668970764447295	17.211533268564587	11.651015537971322	25.468480429016793
2	17.384738174147053	19.49038236158048	36.74403700483923	26.380842459433236
3	20.484389232234463	23.04275715561516	28.00882973389152	28.46402387825886
4	14.25777344197371	14.926895613736823	33.35255961780045	37.46277132648901
5	15.298881904127773	36.81835526382161	32.09548174378092	15.787281088269697
6	35.16854352994419	32.812551039377006	16.662875523608328	15.356029907070473
7	30.487180050428442	31.202997921474356	19.686564745094152	18.62325728300305
8	27.906213306137083	34.11209904627122	18.86828854777489	19.113399099816807
9	27.88188701126279	15.699415227585256	18.15761969792199	38.26107806322997
10-11	26.83498322503739	25.076305821682283	26.016968194504674	22.071742758775653
12-13	26.604039279542736	22.84719716678377	26.0422501946412	24.5065133590323
14-15	24.487450976668217	24.194497991586505	23.127765723076337	28.19028530866894
16-17	24.890312348645285	26.993757063219974	24.09902396983827	24.01690661829647
18-19	24.88274253219145	26.250209613583852	25.39308668430636	23.473961169918336
20-21	25.55057790945986	25.132681174645345	25.18155746510405	24.135183450790745
22-23	25.73769937159107	24.755168629294527	24.818413147262085	24.688718851852315
24-25	25.126955373909986	25.359499158379055	24.85951221596777	24.654033251743186
26-27	24.722053205982483	25.431782902020377	25.1961759127619	24.64998797923524
28-29	24.860835701092746	25.594299450406755	25.1640810526119	24.380783795888604
30-31	25.199584909680468	25.190356064286178	25.02168481920217	24.588374206831183
32-33	24.77075192182596	25.339257479570954	24.98008731903498	24.909903279568105
34-35	24.961719796927195	25.528406299946425	25.059308715479922	24.450565187646454
36-37	25.024855738805122	25.518914542246446	24.97895680961201	24.477272909336413
38-39	24.957115019579764	25.637607153104945	25.114827298378277	24.290450528937015
40-41	24.913186818405226	25.382482554260466	25.202029855424147	24.502300771910164
42-43	24.895496381431457	25.229148312200167	25.05980007590552	24.81555523046286
44-45	24.756430718615057	25.333786720099837	25.18290506383763	24.726877497447475
46-47	24.785812718771922	25.421994642053825	25.129853635723755	24.662339003450498
48-49	24.643060633398225	25.671205070984282	25.138555861545036	24.547178434072457
50-51	24.58724700369041	25.593979723497927	25.25882749542111	24.55994577739055
52-53	24.835107312668345	25.447673964863842	25.12891259404158	24.588306128426233
54-55	24.684562033734746	25.259043257308537	25.104057956134678	24.952336752822035
56-57	24.596580949546322	25.533942869826635	24.959086116639657	24.910390063987382
58-59	24.532187272265464	25.58252565462923	25.163583126221013	24.721703946884293
60-61	24.78733521762031	25.424788017715265	25.207546050044883	24.580330714619546
62-63	24.58766161412492	25.393925851939485	25.339177866264766	24.679234667670823
64-65	24.517200716669326	25.188558928485588	25.26671837732201	25.027521977523076
66-67	24.548258334122206	25.55401803850743	24.989116535713936	24.908607091656425
68-69	24.489644522677125	25.450147833652426	25.163263901170446	24.896943742500007
70-71	24.354197213532235	25.26983147034555	25.474839276491924	24.90113203963029
72-73	24.488010654031225	25.42750843325444	25.232285417888235	24.852195494826102
74-75	24.330801922116084	25.44661665557023	25.29930703656364	24.92327438575004
76-77	24.400431426481695	25.225660677434526	25.286962404475677	25.086945491608105
78-79	24.33905249923069	25.256053820042574	25.352169517347267	25.052724163379477
80-81	24.33944197620101	25.44811896705867	25.27426877731894	24.938170279421378
82-83	24.390323515857688	25.384880397086835	25.398322760948776	24.8264733261067
84-85	24.363733035205122	25.34748492891861	25.283396857666922	25.005385178209345
86-87	24.244528257994606	25.432595716726027	25.338179366733932	24.984696658545435
88-89	24.280259857489657	25.26729220555127	25.328508804596794	25.123939132362278
90-91	24.3395658707155	25.14860287188106	25.379578262200987	25.132252995202453
92-93	24.28919915003557	25.205800692717446	25.56355240507771	24.941447752169267
94-95	24.27628887146252	24.92583160726223	25.68746882761571	25.110410693659542
96-97	24.281003795223498	24.961627919742195	25.535643975932032	25.221724309102278
98-99	24.120716024512284	25.103640201687856	25.444978645950002	25.330665127849866
100-101	24.03990109285629	25.18791348532563	25.554839525117533	25.217345896700543
102-103	23.805015707648167	25.033977115993324	25.837204741754633	25.32380243460388
104-105	23.72712700293091	25.16840358592462	25.735509429562427	25.36895998158204
106-107	23.793353878723657	25.183727766062606	25.640095314155793	25.38282304105795
108-109	23.790500486752066	25.248917084211225	25.684516418276242	25.276066010760466
110-111	23.658238539524145	25.532576406880754	25.601878758265627	25.20730629532948
112-113	23.514274440696468	25.53933740962587	25.790293439661085	25.156094710016585
114-115	23.369354459212833	25.53895945717001	25.776265103459977	25.315420980157178
116-117	23.334082957927667	25.44209061003223	25.80333450425091	25.420491927789197
118-119	23.483828625948636	25.390632553858218	25.701555866085503	25.42398295410765
120-121	23.31892005749488	25.605483670475433	25.833149149962843	25.242447122066846
122-123	23.23049556389001	25.52070075882312	25.831848498818953	25.41695517846791
124-125	23.031299570549773	25.430328173780026	26.031320403201086	25.507051852469115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1074.0
1	856.5
2	567.0
3	387.5
4	249.5
5	212.0
6	208.0
7	213.0
8	225.5
9	235.0
10	233.5
11	236.0
12	262.0
13	299.5
14	333.5
15	356.0
16	407.0
17	462.5
18	506.5
19	564.0
20	646.5
21	739.5
22	850.0
23	966.0
24	1129.0
25	1483.0
26	2062.0
27	2854.5
28	3825.5
29	5094.5
30	6774.0
31	8794.5
32	11805.0
33	14704.5
34	17168.5
35	20167.5
36	23719.5
37	27284.0
38	30171.5
39	33252.5
40	35920.0
41	38152.5
42	41341.5
43	46390.0
44	51420.0
45	55775.5
46	59269.0
47	62735.0
48	68249.0
49	72424.5
50	73937.0
51	74236.0
52	73317.5
53	72801.5
54	73549.0
55	72228.5
56	68215.0
57	63571.5
58	59532.0
59	54597.0
60	47229.5
61	40495.0
62	34572.5
63	27265.0
64	20520.5
65	15955.5
66	12598.5
67	10071.0
68	8274.5
69	6815.0
70	5404.0
71	4168.5
72	3180.0
73	2564.0
74	2085.5
75	1675.5
76	1280.0
77	808.5
78	528.5
79	352.5
80	232.0
81	163.0
82	125.0
83	86.5
84	66.0
85	61.5
86	48.0
87	43.5
88	39.5
89	26.5
90	24.5
91	19.5
92	15.0
93	17.5
94	14.0
95	10.5
96	11.5
97	11.5
98	9.5
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024920062964692426
2	0.012104030582850605
3	0.010324026085372575
4	0.011629362716856464
5	0.01732537710878616
6	0.09730691252879899
7	0.06063881988075156
8	0.05001812637913265
9	0.027293402294663132
10-11	0.05248046593397726
12-13	0.014062035530076438
14-15	0.02830207150990068
16-17	0.03589675736580694
18-19	0.04399577782933198
20-21	0.031031411739366993
22-23	0.039041431978018126
24-25	0.03233674837085088
26-27	0.047704120532411215
28-29	0.04625045019280415
30-31	0.026967068136792158
32-33	0.03162474657185967
34-35	0.04577578232681001
36-37	0.009760357994504532
38-39	0.02127105374486246
40-41	0.043728777154710276
42-43	0.023703726558082436
44-45	0.017414377333660063
46-47	0.024623395548446086
48-49	0.03020074297387725
50-51	0.028658072409396287
52-53	0.02548373105556047
54-55	0.023169725208839028
56-57	0.026551733754047284
58-59	0.01857138025702078
60-61	0.023822393524580973
62-63	0.023466392625085365
64-65	0.02207205576872758
66-67	0.029281073983513598
68-69	0.02296205801746659
70-71	0.04859412278115023
72-73	0.023318058916962196
74-75	0.009671357769630632
76-77	0.016702375534668853
78-79	0.02613639937130241
80-81	0.018838380931642487
82-83	0.024771729256569255
84-85	0.012341364515847678
86-87	0.017770378233155668
88-89	0.023051058242340494
90-91	0.02141938745298563
92-93	0.00842535462139601
94-95	0.012074363841225972
96-97	0.014507036654445946
98-99	0.006615683382293346
100-101	0.01020535911887404
102-103	0.0037676761863284975
104-105	0.004598344951818245
106-107	8.900022487390153E-4
108-109	0.014210369238199608
110-111	0.0012163364066099874
112-113	0.0020173384304751013
114-115	0.013083033056463522
116-117	0.005992681808176035
118-119	0.28180437869239683
120-121	0.001275669889859255
122-123	9.493357319882829E-4
124-125	0.3164847996515938
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1685389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.610441312719196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38113450912348	47.915549013058936
2	10.498478252058087	12.516378434343348
3	3.3877715786947946	6.058396766180523
4	1.6882789506907458	4.0255621323859945
5	0.9696021344724529	2.889920556682871
6	0.621143101729887	2.2215968647481876
7	0.429777212203156	1.793344649990621
8	0.34112244485403226	1.6267567583538063
9	0.23691142747720478	1.2710155269548198
>10	1.3479530726446802	14.278784505026135
>50	0.07350994055705691	2.9425868282084005
>100	0.023920421378673416	2.284176458580243
>500	2.9771558678172503E-4	0.09666176192480332
>1k	9.923852892724169E-5	0.07926974356134422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.933348324926768E-5
2	0.0	0.0	0.0	0.0	5.933348324926768E-5
3	0.0	0.0	0.0	0.0	5.933348324926768E-5
4	0.0	0.0	0.0	0.0	5.933348324926768E-5
5	0.0	0.0	0.0	0.0	5.933348324926768E-5
6	0.0	0.0	0.0	0.0	1.1866696649853536E-4
7	0.0	0.0	0.0	0.0	1.1866696649853536E-4
8	0.0	0.0	0.0	0.0	1.1866696649853536E-4
9	0.0	0.0	0.0	0.0	1.1866696649853536E-4
10-11	0.0	0.0	0.0	0.0	1.483337081231692E-4
12-13	0.0	0.0	0.0	0.0	2.3733393299707071E-4
14-15	0.0	0.0	0.0	0.0	2.670006746217045E-4
16-17	0.0	0.0	0.0	0.0	3.263341578709722E-4
18-19	0.0	0.0	0.0	0.0	5.340013492434091E-4
20-21	0.0	0.0	0.0	8.900022487390152E-5	5.340013492434091E-4
22-23	0.0	5.933348324926768E-5	0.0	2.6700067462170457E-4	5.340013492434091E-4
24-25	0.0	5.933348324926768E-5	0.0	0.0011273361817360859	5.933348324926767E-4
26-27	0.0	5.933348324926768E-5	0.0	0.002136005396973636	7.416685406158459E-4
28-29	0.0	8.900022487390152E-5	0.0	0.006200348999548473	7.713352822404798E-4
30-31	0.0	1.1866696649853536E-4	0.0	0.020974386328616125	8.010020238651136E-4
32-33	0.0	1.1866696649853536E-4	0.0	0.05013679334563119	8.306687654897474E-4
34-35	0.0	1.1866696649853536E-4	0.0	0.09155156465362002	8.900022487390152E-4
36-37	0.0	1.1866696649853536E-4	0.0	0.14771070654905188	0.0010086692152375505
38-39	0.0	1.1866696649853536E-4	0.0	0.2271582406198213	0.0010680026984868183
40-41	0.0	1.1866696649853536E-4	0.0	0.3107591185180395	0.0010680026984868183
42-43	0.0	1.1866696649853536E-4	0.0	0.39967034316706707	0.0010680026984868183
44-45	0.0	1.1866696649853536E-4	0.0	0.5113359586421888	0.001097669440111452
46-47	0.0	1.1866696649853536E-4	0.0	0.6348682707671641	0.0011570029233607198
48-49	0.0	1.1866696649853536E-4	0.0	0.7519628999595939	0.0013053366314838889
50-51	0.0	1.1866696649853536E-4	0.0	0.8604541740808798	0.0013350033731085228
52-53	0.0	1.1866696649853536E-4	0.0	0.9743447951778491	0.0015130038228563258
54-55	0.0	1.1866696649853536E-4	0.0	1.113867481038502	0.0016910042726041288
56-57	0.0	1.1866696649853536E-4	0.0	1.280772569418692	0.0017800044974780303
58-59	0.0	1.1866696649853536E-4	0.0	1.4446516501531694	0.001809671239102664
60-61	0.0	1.1866696649853536E-4	0.0	1.6024193821129722	0.001839337980727298
62-63	0.0	1.7800044974780304E-4	0.0	1.7609287826133908	0.0018986714639765657
64-65	0.0	1.7800044974780304E-4	0.0	1.9453669152937394	0.0018986714639765657
66-67	0.0	1.7800044974780304E-4	0.0	2.165108470507402	0.0018986714639765657
68-69	0.0	1.7800044974780304E-4	0.0	2.3988230610262677	0.0018986714639765657
70-71	0.0	1.7800044974780304E-4	0.0	2.6301346454735377	0.0018986714639765657
72-73	0.0	1.7800044974780304E-4	0.0	2.8499355341704495	0.0021656721385982704
74-75	0.0	1.7800044974780304E-4	0.0	3.0972968258366467	0.002195338880222904
76-77	0.0	1.7800044974780304E-4	0.0	3.406453940306956	0.002195338880222904
78-79	0.0	1.7800044974780304E-4	0.0	3.7387807799861044	0.002195338880222904
80-81	0.0	1.7800044974780304E-4	0.0	4.060457259422009	0.002225005621847538
82-83	0.0	1.7800044974780304E-4	0.0	4.372017379963913	0.0023140058467214395
84-85	0.0	1.7800044974780304E-4	0.0	4.711434570891349	0.0023140058467214395
86-87	0.0	1.7800044974780304E-4	0.0	5.109947911135055	0.0023140058467214395
88-89	0.0	1.7800044974780304E-4	0.0	5.558182710341648	0.0023140058467214395
90-91	0.0	1.7800044974780304E-4	0.0	6.0149615311361355	0.0023140058467214395
92-93	0.0	1.7800044974780304E-4	0.0	6.452783304032481	0.0023140058467214395
94-95	0.0	1.7800044974780304E-4	0.0	6.907426119429996	0.0024326728132199747
96-97	0.0	1.7800044974780304E-4	0.0	7.428581769549938	0.0024623395548446086
98-99	0.0	1.7800044974780304E-4	0.0	8.026188612836561	0.0024920062964692426
100-101	0.0	1.7800044974780304E-4	0.0	8.641684501322839	0.0024920062964692426
102-103	0.0	2.3733393299707071E-4	0.0	9.242080018322179	0.00255133977971851
104-105	0.0	2.3733393299707071E-4	0.0	9.849358219378434	0.0026106732629677777
106-107	0.0	2.3733393299707071E-4	0.0	10.525433594262214	0.002699673487841679
108-109	0.0	2.670006746217045E-4	0.0	11.270958811289264	0.0028183404543402147
110-111	0.0	2.9666741624633837E-4	0.0	12.05214345174912	0.0028480071959648486
112-113	0.0	2.9666741624633837E-4	0.0	12.803008682268604	0.0028480071959648486
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3195	0.0	40.988644	1
GGTATCA	2255	0.0	40.3884	1
GTACATG	8670	0.0	35.633625	1
TCAACGC	3740	0.0	33.893513	4
TACATGG	9165	0.0	33.117622	2
CAACGCA	3900	0.0	32.50204	5
ACATGGG	9395	0.0	31.735802	3
TATCAAC	4290	0.0	30.381433	2
AACGCAG	4230	0.0	29.823076	6
ATCAACG	4280	0.0	29.478178	3
CATGGGG	7665	0.0	29.348654	4
ATGGGGG	4340	0.0	26.32735	5
ACGCAGA	5060	0.0	24.924488	7
CGCAGAG	5295	0.0	23.705248	8
GAGTACA	6300	0.0	21.44851	1
ATGGGTA	840	0.0	21.25385	5
GCAGAGT	6075	0.0	21.053925	9
AGTACAT	6370	0.0	21.02154	2
ATGGGGA	3150	0.0	20.970467	5
CGCCTTA	685	0.0	20.851734	2
>>END_MODULE