FastQCFastQC Report
Mon 15 Aug 2016
SRR2921629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685389
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.1705244308583953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.13967101956877612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.056.8709451
GTATCAA46500.049.781521
TCAACGC59150.038.5184484
CAACGCA61300.037.0360225
ATCAACG62100.036.499843
GTACATG86050.036.3997041
AACGCAG63200.035.9343456
TATCAAC64900.035.1099782
TACATGG89750.034.826412
ACATGGG90850.033.812813
CATGGGG78050.031.177944
ACGCAGA73800.030.690457
CGCAGAG78950.028.7639738
ATGGGGG41200.028.2031255
GCAGAGT85650.026.4443119
GTATAGG4350.026.058761
GAGTACT38250.025.76305412-13
CAGAGTA81200.023.78291110-11
AGAGTAC77600.023.46526710-11
GTACTTT42100.023.39940614-15
Produced by FastQC (version 0.11.5)