##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685389
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82004095197014	33.0	31.0	34.0	30.0	34.0
2	31.985557043507463	33.0	31.0	34.0	30.0	34.0
3	32.05406644994123	34.0	31.0	34.0	30.0	34.0
4	35.45170699464634	37.0	35.0	37.0	35.0	37.0
5	35.32195772014651	37.0	35.0	37.0	33.0	37.0
6	35.36268006970498	37.0	36.0	37.0	33.0	37.0
7	35.25192403652807	37.0	35.0	37.0	33.0	37.0
8	35.28962868512848	37.0	36.0	37.0	33.0	37.0
9	37.01671186889199	39.0	38.0	39.0	34.0	39.0
10-11	36.97599337600993	39.0	38.0	39.0	34.0	39.0
12-13	36.91199272096827	39.0	38.0	39.0	34.0	39.0
14-15	38.35362637349597	41.0	39.0	41.0	34.0	41.0
16-17	38.36077576156009	41.0	39.0	41.0	34.0	41.0
18-19	38.37052009951412	41.0	39.0	41.0	34.0	41.0
20-21	38.30838399918357	41.0	39.0	41.0	34.0	41.0
22-23	38.3099056063615	41.0	39.0	41.0	34.0	41.0
24-25	38.26972645484217	41.0	39.0	41.0	34.0	41.0
26-27	38.19706607792029	41.0	39.0	41.0	34.0	41.0
28-29	38.124948305702716	41.0	39.0	41.0	34.0	41.0
30-31	38.003976826714776	41.0	38.5	41.0	33.5	41.0
32-33	37.88151073728379	41.0	38.0	41.0	33.0	41.0
34-35	37.746021541614425	41.0	38.0	41.0	33.0	41.0
36-37	37.662602224174954	40.5	38.0	41.0	33.0	41.0
38-39	37.57525977682303	40.0	38.0	41.0	33.0	41.0
40-41	37.45026667434047	40.0	38.0	41.0	32.5	41.0
42-43	37.2778364520001	40.0	37.5	41.0	32.0	41.0
44-45	37.11545643171992	40.0	37.0	41.0	31.5	41.0
46-47	36.89555882944531	40.0	37.0	41.0	31.0	41.0
48-49	36.692053585255394	40.0	36.5	41.0	31.0	41.0
50-51	36.43372004919932	40.0	36.0	41.0	30.0	41.0
52-53	36.29215985152389	40.0	35.0	41.0	30.0	41.0
54-55	36.15380722195292	39.5	35.0	41.0	30.0	41.0
56-57	35.94337093691723	39.0	35.0	41.0	30.0	41.0
58-59	35.745200959541094	39.0	35.0	41.0	29.5	41.0
60-61	35.48776662242366	38.5	35.0	41.0	29.0	41.0
62-63	35.13378573136528	37.5	35.0	40.5	28.5	41.0
64-65	34.842369328386496	37.0	35.0	40.0	28.5	41.0
66-67	34.53859761752331	36.5	35.0	39.5	29.0	41.0
68-69	34.17406871648029	36.0	35.0	39.0	28.0	41.0
70-71	33.805223897865716	35.0	35.0	39.0	28.0	40.5
72-73	33.49692355889353	35.0	34.0	37.0	28.0	39.5
74-75	33.18188204622197	35.0	34.0	37.0	27.0	39.0
76-77	32.87331114656617	35.0	34.0	36.5	27.0	39.0
78-79	32.62499458581965	35.0	34.0	36.0	26.0	38.0
80-81	32.384231177490776	35.0	34.0	36.0	26.0	37.0
82-83	32.17683632680645	35.0	34.0	35.0	26.0	37.0
84-85	31.975368001096484	35.0	34.0	35.0	25.5	36.0
86-87	31.85466975279891	35.0	34.0	35.0	25.0	36.0
88-89	31.76168350452032	35.0	34.0	35.0	25.0	36.0
90-91	31.662282713367652	35.0	34.0	35.0	25.0	35.5
92-93	31.591293464001488	35.0	33.5	35.0	25.0	35.0
94-95	31.524829282735322	35.0	33.0	35.0	25.0	35.0
96-97	31.45922395363919	35.0	33.0	35.0	24.0	35.0
98-99	31.38035432769527	35.0	33.0	35.0	24.0	35.0
100-101	31.311892981382933	35.0	33.0	35.0	24.0	35.0
102-103	30.608566330977595	34.0	31.5	35.0	21.5	35.0
104-105	31.132793675525352	35.0	33.0	35.0	23.0	35.0
106-107	31.232774748144195	35.0	33.0	35.0	24.0	35.0
108-109	31.212169119413975	35.0	33.0	35.0	23.5	35.0
110-111	31.13600302363431	35.0	33.0	35.0	23.0	35.0
112-113	31.09269907421966	35.0	33.0	35.0	23.0	35.0
114-115	30.961242182071913	35.0	33.0	35.0	20.0	35.0
116-117	31.143584359456483	35.0	33.0	35.0	23.0	35.0
118-119	31.117596590460717	35.0	33.5	35.0	21.5	35.0
120-121	31.020202457711544	35.0	33.0	35.0	19.0	35.0
122-123	30.868331880651887	35.0	33.0	35.0	13.5	35.0
124-125	30.224579310770395	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24760.0
3	6531.0
4	3518.0
5	2876.0
6	3145.0
7	3582.0
8	3868.0
9	4103.0
10	4518.0
11	4825.0
12	5471.0
13	7063.0
14	9543.0
15	8609.0
16	4753.0
17	4054.0
18	3709.0
19	3642.0
20	3475.0
21	3561.0
22	3950.0
23	4905.0
24	6173.0
25	8033.0
26	9912.0
27	11687.0
28	14233.0
29	17315.0
30	20903.0
31	26730.0
32	35187.0
33	48288.0
34	73908.0
35	134636.0
36	314376.0
37	623064.0
38	216238.0
39	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31836592746542	17.506054835055078	11.021609870998335	27.153969366481167
2	16.778502782503132	19.694390433339485	36.08264856086529	27.444458223292095
3	19.269515505152754	22.967461941196337	28.434551440593008	29.328471113057898
4	13.928651876717272	14.86134942488703	33.25070028094446	37.959298417451244
5	15.182743318976902	36.726947065180816	32.31081587148411	15.779493744358172
6	35.39433285496548	33.02347287939252	16.079988172419053	15.50220609322295
7	30.776929976984118	31.211542491521165	19.083354061676864	18.92817346981785
8	28.641287604244763	33.33962556474261	18.58220592573861	19.436880905274016
9	28.101469731089423	14.765212209416203	18.43467288066581	38.69864517882856
10-11	27.19779396070322	24.52547385820278	25.91998209329713	22.356750087796872
12-13	27.06487731609288	22.136950029980824	26.370004927541395	24.428167726384906
14-15	24.693461966604822	23.467458256029687	23.772734693877553	28.066345083487942
16-17	24.608345404032008	26.4879609621732	24.653194975423265	24.250498658371527
18-19	24.604679943670916	25.392871610943512	26.60759730344548	23.39485114194009
20-21	25.309611510441254	24.357816690994433	26.391200578999864	23.94137121956445
22-23	25.255780008128948	24.028457250338953	26.13385469357724	24.581908047954855
24-25	24.667464542110675	24.61785939551738	26.173123148336597	24.54155291403535
26-27	24.340916602238252	24.788691611687426	26.448126275341433	24.422265510732892
28-29	24.4138322244381	24.971830125122306	26.408627250400666	24.205710400038924
30-31	24.866036807762892	24.537499903580915	26.274141113631522	24.32232217502467
32-33	24.467028992602167	24.64050751740274	26.216471054572597	24.675992435422494
34-35	24.67445274358043	24.777816148425575	26.31559897372362	24.23213213427037
36-37	24.68124799180672	24.800100398538287	26.190489611985164	24.328161997669827
38-39	24.661887638142712	24.91153911992735	26.30923140867814	24.1173418332518
40-41	24.64027213556462	24.669079439031908	26.354321525701245	24.336326899702225
42-43	24.5594771815796	24.561346255059107	26.08829028431871	24.790886279042585
44-45	24.577844854108484	24.664742787753354	26.06584958676754	24.691562771370617
46-47	24.676706746287927	24.786033683970683	25.893721755971594	24.643537813769793
48-49	24.49153781866869	25.09102700534577	25.824434843413968	24.593000332571574
50-51	24.57267340784947	25.0060817794634	25.832284102762493	24.588960709924635
52-53	24.802231912804928	24.949232231132562	25.656109442997842	24.592426413064665
54-55	24.497256576413857	24.78951856135069	25.698613246401393	25.014611615834063
56-57	24.46919730480867	25.02563232330904	25.55774036829851	24.94743000358378
58-59	24.450047556238292	25.02309577761125	25.744983454701124	24.781873211449334
60-61	24.57760468212661	24.939331477380875	25.860692018692433	24.622371821800083
62-63	24.485640109613815	24.870985417755076	25.933896473270135	24.70947799936097
64-65	24.369655907330593	24.704949421172202	25.853081988787157	25.072312682710045
66-67	24.298673638222194	25.10792431961916	25.692648507750288	24.90075353440836
68-69	24.399512988746768	25.01504110047455	25.690674280370864	24.894771630407824
70-71	24.234138231589263	24.8493374526593	25.925201837676642	24.991322478074796
72-73	24.47749663570966	25.036312975124424	25.60536458939044	24.880825799775476
74-75	24.259630001910633	25.076959317773746	25.624901353226058	25.03850932708956
76-77	24.334517849927344	24.815484197116692	25.6224942252591	25.22750372769686
78-79	24.207562131964547	24.929444883348577	25.66648738017942	25.196505604507458
80-81	24.182191767004532	25.249482463692356	25.45468731550995	25.113638453793158
82-83	24.233162325001274	25.092031201603515	25.590400036076705	25.084406437318503
84-85	24.210362052996363	25.002299210673556	25.437665876924886	25.3496728594052
86-87	23.988171276779426	25.192789320447684	25.51473280055821	25.304306602214684
88-89	23.989056399238503	24.95917065021685	25.52136224214467	25.530410708399977
90-91	23.926765129597502	24.94926936011753	25.658608307246116	25.465357203038852
92-93	23.95159218435625	25.052584299529663	25.74779472276133	25.248028793352752
94-95	23.908520454496234	24.75909142288033	25.820005891991432	25.512382230632006
96-97	23.907117767123935	24.79915270328001	25.668877865856555	25.624851663739495
98-99	23.84899776252107	24.8596013122271	25.539863733913855	25.75153719133797
100-101	23.666257463410524	24.92555131189298	25.691665247607524	25.716525977088967
102-103	23.519036979834333	24.766927273002366	25.920268286177855	25.793767460985446
104-105	23.531689123401183	24.838242091291683	25.84462696742414	25.785441817882994
106-107	23.657778848424506	24.891745353267932	25.587968989745917	25.86250680856164
108-109	23.606386570378003	24.92845695461296	25.61709770498573	25.848058770023304
110-111	23.401561955336682	25.171778003574847	25.53875592408274	25.887904117005732
112-113	23.242863347462656	25.23395261851305	25.66073962966303	25.862444404361263
114-115	23.127509435507175	25.26793517698288	25.64111311987915	25.9634422676308
116-117	23.10449767942479	25.147268414188556	25.654757030850416	26.093476875536236
118-119	23.211734312438736	25.141335795337223	25.482808298345503	26.164121593878537
120-121	22.97934869579845	25.276865252531906	25.606145698067724	26.137640353601928
122-123	22.86166244304639	25.426480823419052	25.487921593152336	26.22393514038222
124-125	22.65773893054275	25.3621658224521	25.594833436539865	26.385261810465288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	20.0
2	25.5
3	68.0
4	82.5
5	90.5
6	222.0
7	588.5
8	949.0
9	999.0
10	789.5
11	569.0
12	516.0
13	549.0
14	596.0
15	625.0
16	663.0
17	698.0
18	756.5
19	819.0
20	862.5
21	920.5
22	1012.5
23	1154.5
24	1336.0
25	1613.5
26	2067.0
27	2878.5
28	3795.0
29	4931.5
30	6801.0
31	8900.0
32	11684.5
33	14305.5
34	16637.5
35	19805.5
36	23574.5
37	26926.0
38	29703.5
39	32925.5
40	35764.0
41	37955.5
42	40798.0
43	45844.0
44	51022.5
45	55760.0
46	59382.5
47	62717.5
48	68484.5
49	72930.0
50	74703.5
51	74988.5
52	74211.5
53	73573.5
54	74173.5
55	73028.5
56	68960.0
57	64005.5
58	59782.5
59	55098.0
60	47465.5
61	40301.0
62	34156.5
63	26737.5
64	19953.5
65	15507.5
66	12307.5
67	9714.5
68	7867.0
69	6442.0
70	5134.5
71	3939.0
72	2914.0
73	2334.0
74	1918.0
75	1486.0
76	1053.0
77	619.0
78	355.5
79	216.0
80	137.0
81	72.5
82	39.5
83	20.0
84	13.5
85	9.0
86	5.5
87	4.5
88	3.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02207205576872758
2	0.002136005396973636
3	0.03334541758608844
4	0.16910042726041288
5	0.025513397797185103
6	0.07060684506662854
7	0.05340013492434091
8	0.045805449068434645
9	0.035066088600317194
10-11	0.06547449876556688
12-13	0.058087480101033054
14-15	0.060164152014757416
16-17	0.05156079694361362
18-19	0.03835909692065155
20-21	0.024593728806821452
22-23	0.003204008095460454
24-25	0.004746678659941414
26-27	0.0033226750619589898
28-29	0.004628011693442878
30-31	0.0022546723634721717
32-33	0.009849358219378435
34-35	0.00433134427719654
36-37	0.00625968248279774
38-39	0.002343672588346073
40-41	0.0031150078705865533
42-43	0.003678675961454596
44-45	0.0046576784350675126
46-47	0.004568678210193611
48-49	0.002225005621847538
50-51	0.001602004047730227
52-53	0.001097669440111452
54-55	0.00510267955943702
56-57	0.0010086692152375507
58-59	9.493357319882829E-4
60-61	5.933348324926768E-5
62-63	0.0010383359568621844
64-65	0.0
66-67	0.003827009669577765
68-69	4.153343827448737E-4
70-71	0.0
72-73	0.0024326728132199747
74-75	0.004924679109689217
76-77	0.0018986714639765657
78-79	2.670006746217045E-4
80-81	0.0
82-83	0.005399346975683359
84-85	0.0016613375309794949
86-87	0.0
88-89	0.0012163364066099874
90-91	0.0010086692152375507
92-93	0.0
94-95	0.0029963409040880177
96-97	0.0017206710142287627
98-99	5.933348324926767E-4
100-101	0.0
102-103	0.001453670339607058
104-105	0.0
106-107	6.526683157419444E-4
108-109	0.002373339329970707
110-111	8.900022487390152E-5
112-113	2.9666741624633837E-4
114-115	0.0
116-117	0.001839337980727298
118-119	0.0024326728132199747
120-121	0.0028183404543402147
122-123	0.0015426705644809597
124-125	0.11104261390100446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1685389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.45044090717148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20746827608929	44.14846066418867
2	11.50180019402883	12.98563384358236
3	3.862489296456004	6.54117671352516
4	1.8671140354492612	4.21597642100316
5	1.1010308403183533	3.1076838194182286
6	0.7005884591751668	2.3729116448948444
7	0.48132408972348445	1.9019669958893566
8	0.35564066818211587	1.6060858018721214
9	0.2827128968977485	1.436334091201945
>10	1.5261826180312017	15.345655005676928
>50	0.08466143969683015	3.228633255483638
>100	0.027933236859601928	2.46561721146938
>500	7.377643644835498E-4	0.23899499392144857
>1k	3.1618472763580703E-4	0.4048695378729046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2874	0.1705244308583953	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2354	0.13967101956877612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.933348324926768E-5
7	0.0	0.0	0.0	0.0	5.933348324926768E-5
8	0.0	0.0	0.0	0.0	5.933348324926768E-5
9	0.0	0.0	0.0	5.933348324926768E-5	5.933348324926768E-5
10-11	0.0	0.0	0.0	5.933348324926768E-5	5.933348324926768E-5
12-13	0.0	0.0	0.0	5.933348324926768E-5	5.933348324926768E-5
14-15	0.0	0.0	0.0	5.933348324926768E-5	1.483337081231692E-4
16-17	0.0	0.0	0.0	5.933348324926768E-5	3.856676411202399E-4
18-19	0.0	0.0	0.0	5.933348324926768E-5	4.153343827448737E-4
20-21	0.0	0.0	0.0	1.483337081231692E-4	5.340013492434091E-4
22-23	0.0	0.0	0.0	3.263341578709722E-4	5.933348324926768E-4
24-25	0.0	0.0	0.0	0.0011866696649853537	6.526683157419444E-4
26-27	0.0	0.0	0.0	0.0022843391050968056	6.526683157419444E-4
28-29	0.0	0.0	0.0	0.0062893492244223735	6.526683157419444E-4
30-31	0.0	0.0	0.0	0.02195338880222904	6.823350573665783E-4
32-33	0.0	0.0	0.0	0.05164979716848751	7.120017989912121E-4
34-35	0.0	0.0	0.0	0.0937172367922183	7.120017989912121E-4
36-37	0.0	0.0	0.0	0.1499357121708994	7.120017989912121E-4
38-39	0.0	0.0	0.0	0.23000624781578616	7.416685406158459E-4
40-41	0.0	2.966674162463384E-5	0.0	0.3146454616708665	8.306687654897474E-4
42-43	0.0	5.933348324926768E-5	0.0	0.40444668856863314	8.306687654897474E-4
44-45	0.0	8.900022487390152E-5	0.0	0.5167056388762475	8.306687654897474E-4
46-47	0.0	1.1866696649853536E-4	0.0	0.6416619545992053	8.306687654897474E-4
48-49	0.0	1.1866696649853536E-4	0.0	0.7593795853657523	9.493357319882829E-4
50-51	0.0	1.1866696649853536E-4	0.0	0.8688498619606513	9.493357319882829E-4
52-53	0.0	1.1866696649853536E-4	0.0	0.9841051531723537	0.0011273361817360859
54-55	0.0	1.1866696649853536E-4	0.0	1.1261198453294758	0.0011866696649853535
56-57	0.0	1.1866696649853536E-4	0.0	1.2938556024751557	0.0012163364066099874
58-59	0.0	1.1866696649853536E-4	0.0	1.4590400198411169	0.0012460031482346213
60-61	0.0	1.1866696649853536E-4	0.0	1.6173714198917877	0.0012460031482346213
62-63	0.0	1.7800044974780304E-4	0.0	1.7782541597221768	0.0013053366314838889
64-65	0.0	1.7800044974780304E-4	0.0	1.9657776335314874	0.0013646701147331565
66-67	0.0	1.7800044974780304E-4	0.0	2.190503201338089	0.0013646701147331565
68-69	0.0	1.7800044974780304E-4	0.0	2.42694713208642	0.0013646701147331565
70-71	0.0	1.7800044974780304E-4	0.0	2.6610177235047816	0.0013646701147331565
72-73	0.0	1.7800044974780304E-4	0.0	2.885802624794632	0.001631670789354861
74-75	0.0	1.7800044974780304E-4	0.0	3.138058928828894	0.0016910042726041288
76-77	0.0	1.7800044974780304E-4	0.0	3.450420051394663	0.0017206710142287627
78-79	0.0	1.7800044974780304E-4	0.0	3.7875825699586265	0.0017206710142287627
80-81	0.0	1.7800044974780304E-4	0.0	4.115044064011335	0.0017503377558533966
82-83	0.0	1.7800044974780304E-4	0.0	4.43351653535178	0.001839337980727298
84-85	0.0	1.7800044974780304E-4	0.0	4.77981641033613	0.0018690047223519318
86-87	0.0	1.7800044974780304E-4	0.0	5.187081439359103	0.0019283382056011996
88-89	0.0	1.7800044974780304E-4	0.0	5.645758931617568	0.0019580049472258335
90-91	0.0	1.7800044974780304E-4	0.0	6.113633113779668	0.0019580049472258335
92-93	0.0	1.7800044974780304E-4	0.0	6.562342580852254	0.002047005172099735
94-95	0.0	1.7800044974780304E-4	0.0	7.026656754019399	0.0021656721385982704
96-97	0.0	1.7800044974780304E-4	0.0	7.556267425502362	0.002225005621847538
98-99	0.0	1.7800044974780304E-4	0.0	8.163041291950998	0.0022843391050968056
100-101	0.0	1.7800044974780304E-4	0.0	8.789039206972397	0.0023140058467214395
102-103	0.0	1.7800044974780304E-4	0.0	9.399373082415988	0.0024326728132199747
104-105	0.0	1.7800044974780304E-4	0.0	10.014008635395152	0.0025216730380938765
106-107	2.966674162463384E-5	1.7800044974780304E-4	0.0	10.69993336849831	0.002581006521343144
108-109	5.933348324926768E-5	2.3733393299707071E-4	0.0	11.457859283524456	0.0026106732629677777
110-111	5.933348324926768E-5	2.9666741624633837E-4	0.0	12.2544409628875	0.0026106732629677777
112-113	5.933348324926768E-5	2.9666741624633837E-4	0.0	13.022364569841146	0.0026106732629677777
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2780	0.0	56.870945	1
GTATCAA	4650	0.0	49.78152	1
TCAACGC	5915	0.0	38.518448	4
CAACGCA	6130	0.0	37.036022	5
ATCAACG	6210	0.0	36.49984	3
GTACATG	8605	0.0	36.399704	1
AACGCAG	6320	0.0	35.934345	6
TATCAAC	6490	0.0	35.109978	2
TACATGG	8975	0.0	34.82641	2
ACATGGG	9085	0.0	33.81281	3
CATGGGG	7805	0.0	31.17794	4
ACGCAGA	7380	0.0	30.69045	7
CGCAGAG	7895	0.0	28.763973	8
ATGGGGG	4120	0.0	28.203125	5
GCAGAGT	8565	0.0	26.444311	9
GTATAGG	435	0.0	26.05876	1
GAGTACT	3825	0.0	25.763054	12-13
CAGAGTA	8120	0.0	23.782911	10-11
AGAGTAC	7760	0.0	23.465267	10-11
GTACTTT	4210	0.0	23.399406	14-15
>>END_MODULE