FastQCFastQC Report
Mon 15 Aug 2016
SRR2921628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587857
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16490.10385066161499429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36850.041.8487241
GGTATCA25300.038.12541
GTACATG92150.035.6667631
TCAACGC43850.035.1582154
TACATGG93400.034.4794852
CAACGCA44500.034.243385
AACGCAG46300.033.0354586
ACATGGG96850.032.758523
TATCAAC46800.032.68872
CATGGGG82950.032.578884
ATCAACG47150.032.192533
ATGGGGG52850.031.7614845
ACGCAGA56200.027.2048977
TGGGGGG43400.026.3293236
CGCAGAG58500.026.1353038
TACGTCT3252.3646862E-1123.8060996
CTACGTC4800.023.5618325
CTCTACG4800.023.5618323
TCTACGT3800.023.4965634
GCAGAGT65850.022.5850379
Produced by FastQC (version 0.11.5)