##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921628_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587857
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.631028486822178	31.0	31.0	34.0	26.0	34.0
2	29.97243328586894	31.0	31.0	34.0	26.0	34.0
3	30.04898551947688	31.0	31.0	34.0	26.0	34.0
4	33.47437017313272	37.0	35.0	37.0	28.0	37.0
5	33.5233053102389	37.0	35.0	37.0	28.0	37.0
6	33.48732725931869	37.0	35.0	37.0	28.0	37.0
7	33.42474479754789	37.0	35.0	37.0	27.0	37.0
8	33.35911294279019	37.0	35.0	37.0	27.0	37.0
9	35.04810004931174	39.0	37.0	39.0	27.0	39.0
10-11	35.072951468551636	39.0	37.0	39.0	27.0	39.0
12-13	34.9968145746122	39.0	37.0	39.0	27.0	39.0
14-15	36.267769074922995	40.0	38.0	41.0	27.0	41.0
16-17	36.24015733154812	40.0	38.0	41.0	27.0	41.0
18-19	36.20294176364749	40.0	38.0	41.0	27.0	41.0
20-21	36.268315723645145	40.0	38.0	41.0	27.0	41.0
22-23	36.21368706375952	40.0	37.5	41.0	27.0	41.0
24-25	36.22090181924443	40.0	37.0	41.0	27.0	41.0
26-27	36.1922364545422	40.0	37.0	41.0	27.0	41.0
28-29	36.14003653981435	40.0	37.0	41.0	27.0	41.0
30-31	36.04272173123903	40.0	37.0	41.0	27.0	41.0
32-33	35.944882631118546	40.0	37.0	41.0	27.0	41.0
34-35	35.81766084729292	40.0	36.5	41.0	26.0	41.0
36-37	35.71809835520453	40.0	36.0	41.0	25.0	41.0
38-39	35.5984225909512	40.0	36.0	41.0	25.0	41.0
40-41	35.48649028218536	40.0	36.0	41.0	24.5	41.0
42-43	35.248875371018926	40.0	35.0	41.0	23.5	41.0
44-45	34.93250210818732	40.0	35.0	41.0	23.0	41.0
46-47	34.68660874373448	39.0	35.0	41.0	22.0	41.0
48-49	34.49762604567036	39.0	35.0	41.0	20.5	41.0
50-51	34.30360951899321	39.0	34.0	41.0	19.5	41.0
52-53	34.11181328041505	39.0	34.0	40.0	18.5	41.0
54-55	33.87863957522623	38.0	34.0	40.0	17.0	41.0
56-57	33.63653496505038	38.0	34.0	40.0	14.5	41.0
58-59	33.64514184841582	38.0	34.0	40.0	14.5	41.0
60-61	33.42972478000223	37.5	34.0	40.0	13.0	41.0
62-63	33.12564859430037	37.0	33.0	40.0	9.0	41.0
64-65	32.83326458238997	36.5	33.0	39.5	8.0	41.0
66-67	32.51022699147342	36.0	33.0	39.0	7.0	41.0
68-69	32.17460419924464	35.5	33.0	39.0	3.5	40.5
70-71	31.804282123642118	35.0	32.0	38.0	2.0	40.0
72-73	31.456931889962384	35.0	32.0	37.0	2.0	39.5
74-75	31.0848316315638	35.0	32.0	37.0	2.0	39.0
76-77	30.77910038498429	35.0	32.0	36.0	2.0	39.0
78-79	30.5053685564884	35.0	31.0	36.0	2.0	37.5
80-81	30.243328901783975	35.0	31.0	35.0	2.0	37.0
82-83	30.022126992544038	35.0	31.0	35.0	2.0	37.0
84-85	29.809294854637415	35.0	31.0	35.0	2.0	36.0
86-87	29.630651248821525	35.0	31.0	35.0	2.0	36.0
88-89	29.475013178138838	35.0	31.0	35.0	2.0	36.0
90-91	29.29431176736948	34.0	30.0	35.0	2.0	35.0
92-93	29.21214126964834	34.0	30.0	35.0	2.0	35.0
94-95	29.090355113842115	34.0	30.0	35.0	2.0	35.0
96-97	29.001280027105715	34.0	30.0	35.0	2.0	35.0
98-99	28.912183527861764	34.0	30.0	35.0	2.0	35.0
100-101	28.811022969952585	34.0	30.0	35.0	2.0	35.0
102-103	27.95259522740398	33.5	28.0	34.5	2.0	35.0
104-105	28.832352661480222	34.0	30.0	35.0	2.0	35.0
106-107	29.090779585315303	35.0	31.0	35.0	2.0	35.0
108-109	29.091511704139606	35.0	31.0	35.0	2.0	35.0
110-111	29.029503601394836	35.0	31.0	35.0	2.0	35.0
112-113	28.946200130112473	35.0	31.0	35.0	2.0	35.0
114-115	28.829253515902252	35.0	31.0	35.0	2.0	35.0
116-117	28.71016659560653	35.0	30.0	35.0	2.0	35.0
118-119	28.43496202743698	34.5	30.0	35.0	2.0	35.0
120-121	28.31282067591729	34.0	30.0	35.0	2.0	35.0
122-123	28.088527493344806	34.0	29.5	35.0	2.0	35.0
124-125	27.345225986974896	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71731.0
3	12347.0
4	6215.0
5	5018.0
6	5370.0
7	5910.0
8	5935.0
9	6342.0
10	6686.0
11	7391.0
12	7283.0
13	7473.0
14	7832.0
15	7073.0
16	5821.0
17	5167.0
18	4585.0
19	4287.0
20	4285.0
21	4344.0
22	5093.0
23	7036.0
24	10671.0
25	13944.0
26	14674.0
27	15350.0
28	18485.0
29	22051.0
30	26633.0
31	32880.0
32	43410.0
33	58890.0
34	93246.0
35	148503.0
36	293164.0
37	464829.0
38	127782.0
39	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03689266323263	17.45801266565162	12.663698791074705	24.84139588004105
2	16.384650510967294	19.298848945785505	39.164564536192856	25.151936007054342
3	19.906669379204615	24.06598521498858	27.986589313146343	28.040756092660462
4	13.629738711115927	15.247520262422624	34.908053824855074	36.21468720160638
5	14.546298594217888	37.819872435257054	31.998959426449968	15.63486954407509
6	33.761091625208344	33.53686365779673	17.78259532052559	14.91944939646934
7	29.601571356643642	32.915119896604025	19.305076724626304	18.178232022126032
8	27.37733385756916	35.09910073393016	18.656179429792726	18.867385978707958
9	28.27557316585352	16.653070480738037	17.964918690608553	37.10643766279989
10-11	26.57385222466388	25.87166339799784	26.105463796539148	21.44902058079913
12-13	26.310104144457487	23.258424949319764	26.38656933626135	24.0449015699614
14-15	24.150458564174574	24.34196663034943	23.8277296750253	27.679845130450698
16-17	24.59050268630238	27.303863386656186	24.384746036066005	23.720887890975433
18-19	24.563701516046464	26.667580232329197	25.760220515849575	23.008497735774757
20-21	25.41318910392197	25.60807016096035	25.337057292888872	23.641683442228807
22-23	25.351630399515134	25.043077677163446	25.354402491354538	24.250889431966883
24-25	24.72607484027529	25.261152222853255	25.37410845373126	24.638664483140197
26-27	24.39617658576203	25.41488001345838	25.571327354562413	24.61761604621718
28-29	24.622174281356944	26.183913774489405	25.24045602767997	23.953455916473683
30-31	25.3779421088787	25.504434640433328	25.329436506639126	23.78818674404885
32-33	24.800307676897678	25.44202567816959	25.449900291644184	24.30776635328855
34-35	24.738750380797054	25.95270146172513	25.38302591814226	23.925522239335557
36-37	24.714344424272102	26.01420601716626	25.320816334242924	23.95063322431871
38-39	24.894702412466703	25.972166098853904	25.221752483190023	23.911379005489366
40-41	24.881140946109248	25.654339095776518	25.381086131703267	24.083433826410968
42-43	24.768425342724193	25.601653164312964	25.176393589673328	24.45352790328951
44-45	24.360139242368078	25.727556490444805	25.614649612321553	24.29765465486556
46-47	24.315495097731972	25.641496472867836	25.41648569320108	24.626522736199114
48-49	24.282890223443804	25.876555000412633	25.447829409359706	24.392725366783864
50-51	24.412535392138118	25.83754437699285	25.50599457538411	24.24392565548492
52-53	24.467031968475318	25.814283181029758	25.38088493245003	24.337799918044894
54-55	24.18730958535474	25.33317543202648	25.80149349019316	24.67802149242562
56-57	24.462572521055517	25.609680812865754	25.292824431327837	24.634922234750892
58-59	24.319829123464093	25.94126024594682	25.529532703569398	24.209377927019695
60-61	24.833470185580467	25.681828131845563	25.533983609856765	23.950718072717205
62-63	24.653327978960466	25.717651320089136	25.57950850006693	24.04951220088347
64-65	24.435286843952746	25.36734425841231	25.59534289401103	24.602026003623916
66-67	24.455035507815627	25.642640120902332	25.47028248132005	24.432041889961994
68-69	24.233676923992732	25.559661593594203	25.637645870124683	24.569015612288382
70-71	24.25463919896692	25.5670954674893	25.77747911994231	24.40078621360147
72-73	24.294689803356615	26.022634430514024	25.27916658530148	24.403509180827886
74-75	24.280927502275297	25.864502536680302	25.365039475217372	24.489530485827025
76-77	24.17556668422997	25.45369854505932	25.559834226773216	24.810900543937496
78-79	24.153024569161694	25.440397616944075	25.812376079359606	24.594201734534625
80-81	24.351574258931887	25.680975763697226	25.37859282488529	24.588857152485595
82-83	24.47682179278735	25.824287966759496	25.460342017851147	24.238548222602006
84-85	24.459691911172364	25.94480300870653	25.16595188261459	24.429553197506518
86-87	24.121211701283	26.08786575957777	25.336528574024637	24.454393965114598
88-89	24.099251331508643	25.675203230266742	25.512776498675578	24.712768939549036
90-91	24.57709728534079	25.353407111323044	25.53202010317412	24.537475500162046
92-93	24.332482327751674	25.846153264772116	25.507210138754743	24.314154268721467
94-95	24.383297106401365	25.6049561718158	25.471522230277316	24.540224491505512
96-97	24.019682409507872	25.706431142903124	25.518381240234934	24.75550520735407
98-99	24.009907148041954	25.653057721891305	25.344978798514244	24.992056331552504
100-101	23.912152793195894	25.869747119790816	25.530039425239902	24.688060661773388
102-103	23.71507522259748	25.77656015052392	25.666469851916585	24.841894774962014
104-105	23.994207040668805	25.762168095959826	25.522337816190372	24.721287047181
106-107	23.888444890332355	25.702981969879897	25.739226107367035	24.66934703242071
108-109	23.918362462227442	25.638476279857837	25.769331911925185	24.673829345989535
110-111	23.687720781016093	26.16179072179687	25.61812386598305	24.53236463120399
112-113	23.641747259751156	26.284849843684267	25.579728582477017	24.493674314087563
114-115	23.755611718670036	26.362535528418796	25.46699104801946	24.414861704891702
116-117	23.641550455167824	26.036928615425698	25.622037504267002	24.699483425139473
118-119	23.60842838778617	25.857242595131027	25.776816155009886	24.757512862072918
120-121	23.6267805657756	26.00769096091667	25.842088424637776	24.523440048669954
122-123	23.55165242933185	26.147090016399595	25.764369799552096	24.536887754716457
124-125	23.474917232061998	25.997613857442353	25.73379253573923	24.79367637475642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	469.0
1	389.0
2	284.0
3	239.5
4	217.5
5	200.5
6	203.5
7	237.5
8	271.0
9	314.0
10	326.5
11	328.0
12	356.0
13	401.0
14	459.0
15	520.0
16	579.5
17	639.5
18	709.0
19	780.5
20	873.0
21	1006.5
22	1181.5
23	1424.0
24	1737.0
25	2160.5
26	2842.5
27	3891.0
28	4952.5
29	6208.0
30	7890.0
31	9665.5
32	12073.5
33	14578.5
34	16819.0
35	19290.0
36	22386.5
37	25504.5
38	28238.5
39	31194.5
40	34122.5
41	36782.5
42	39930.5
43	44835.5
44	50058.0
45	55023.0
46	58538.0
47	62182.5
48	67758.5
49	72267.0
50	74434.0
51	74342.0
52	72599.5
53	70665.5
54	69739.0
55	67363.5
56	63312.5
57	58652.0
58	53739.0
59	48055.5
60	40497.0
61	33589.5
62	27695.5
63	21382.5
64	15887.0
65	12071.5
66	9315.0
67	7310.5
68	5631.0
69	4337.5
70	3408.0
71	2554.5
72	1984.0
73	1585.0
74	1251.0
75	976.0
76	687.0
77	442.5
78	280.0
79	199.0
80	147.0
81	105.0
82	81.5
83	62.5
84	54.5
85	51.5
86	42.5
87	40.5
88	36.5
89	23.0
90	20.0
91	22.0
92	15.5
93	10.5
94	11.0
95	8.5
96	9.0
97	9.5
98	5.0
99	2.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026072876839665034
2	0.01146198933531168
3	0.010580297847980013
4	0.011902835078977514
5	0.017067028076205856
6	0.09604139415577095
7	0.05970310928503007
8	0.049437701253954226
9	0.02531714127909503
10-11	0.05161044099059298
12-13	0.0130049494381421
14-15	0.028749440283350452
16-17	0.03432299004255421
18-19	0.04216374648346797
20-21	0.03013495547772879
22-23	0.038290601735546714
24-25	0.03252811808620046
26-27	0.04669815984688797
28-29	0.04512371076236714
30-31	0.025915431931212953
32-33	0.030071977514347953
34-35	0.045690512432794636
36-37	0.009415205525434596
38-39	0.02094017282412711
40-41	0.04301394898910922
42-43	0.022420154963576695
44-45	0.01672064927761127
46-47	0.023774181176264616
48-49	0.030103466496038372
50-51	0.029190286027016284
52-53	0.02534863026078545
54-55	0.023144401542456276
56-57	0.024372471828382532
58-59	0.017507873819871688
60-61	0.023837159139645445
62-63	0.023270357469217948
64-65	0.02188484227483961
66-67	0.028969863155183368
68-69	0.022514621908647944
70-71	0.04764282929760048
72-73	0.02292397867062336
74-75	0.009572650433886679
76-77	0.016783627240992104
78-79	0.025159696370642946
80-81	0.01892487799594044
82-83	0.02493927349881003
84-85	0.012469636749405015
86-87	0.018106164471989605
88-89	0.023427802377670028
90-91	0.021821864311458775
92-93	0.00802969033105626
94-95	0.011336033408550016
96-97	0.015524067973375438
98-99	0.007210976807105425
100-101	0.01061178682967043
102-103	0.004376968454967922
104-105	0.004251012528206255
106-107	8.816914873316678E-4
108-109	0.014453442595901268
110-111	0.0017633829746633357
112-113	0.002834008352137504
114-115	0.013823662962092934
116-117	0.00573099466765584
118-119	0.2785200430517358
120-121	0.0011965813042358348
122-123	8.187135239508343E-4
124-125	0.31284303309428996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1587857.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7582369048641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26103248203492	53.918376664152035
2	9.229498264645123	11.953720702698421
3	2.9862719503465285	5.8015711926867315
4	1.3609090678457956	3.5252028728614317
5	0.7836388613323052	2.5373535515007686
6	0.4967603088342518	1.9301593058653161
7	0.35965271193853243	1.6303332866234632
8	0.2706563310503714	1.4021781444769021
9	0.1943530251588866	1.1327363321774575
>10	0.9853389526866931	11.110992232997887
>50	0.04965976996100355	2.208196872923452
>100	0.021160660631173944	2.3406464705174503
>500	8.735019827154872E-4	0.3376388449420819
>1k	1.9411155171455272E-4	0.17089352557660475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1649	0.10385066161499429	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.297796338083342E-5
7	0.0	0.0	0.0	0.0	6.297796338083342E-5
8	0.0	0.0	0.0	0.0	6.297796338083342E-5
9	0.0	0.0	0.0	0.0	1.8893389014250023E-4
10-11	0.0	0.0	0.0	0.0	1.8893389014250023E-4
12-13	0.0	0.0	0.0	6.297796338083342E-5	1.8893389014250023E-4
14-15	0.0	0.0	0.0	9.446694507125013E-5	2.5191185352333367E-4
16-17	0.0	0.0	0.0	1.2595592676166683E-4	3.148898169041671E-4
18-19	0.0	0.0	0.0	2.5191185352333367E-4	3.148898169041671E-4
20-21	0.0	0.0	0.0	2.5191185352333367E-4	3.4637879859458374E-4
22-23	0.0	0.0	0.0	4.093567619754172E-4	4.408457436658339E-4
24-25	0.0	0.0	0.0	8.187135239508343E-4	5.353126887370841E-4
26-27	0.0	0.0	0.0	0.0011336033408550015	6.297796338083342E-4
28-29	0.0	0.0	0.0	0.0023931626084716696	6.297796338083342E-4
30-31	0.0	0.0	0.0	0.009415205525434595	6.297796338083342E-4
32-33	0.0	0.0	0.0	0.025065229425571697	6.297796338083342E-4
34-35	0.0	0.0	0.0	0.04364372862291756	6.927575971891676E-4
36-37	0.0	0.0	0.0	0.06905533684708384	6.927575971891676E-4
38-39	0.0	0.0	0.0	0.11591094160242389	7.242465788795842E-4
40-41	0.0	0.0	0.0	0.1741655577296948	8.187135239508343E-4
42-43	0.0	0.0	0.0	0.2244534614892903	8.187135239508343E-4
44-45	0.0	0.0	0.0	0.2748043432122666	8.187135239508343E-4
46-47	0.0	0.0	0.0	0.33327938221137043	8.502025056412511E-4
48-49	0.0	0.0	0.0	0.4025236529485967	9.446694507125012E-4
50-51	6.297796338083342E-5	0.0	0.0	0.4749168218548647	9.446694507125012E-4
52-53	6.297796338083342E-5	0.0	0.0	0.551938871069624	9.446694507125012E-4
54-55	6.297796338083342E-5	0.0	0.0	0.6391003723886974	9.446694507125012E-4
56-57	6.297796338083342E-5	0.0	0.0	0.7235538212823951	9.446694507125012E-4
58-59	6.297796338083342E-5	0.0	0.0	0.8254521660325835	9.446694507125012E-4
60-61	6.297796338083342E-5	0.0	0.0	0.9325461927616907	9.446694507125012E-4
62-63	6.297796338083342E-5	0.0	0.0	1.0558570450613627	0.0010076474140933347
64-65	6.297796338083342E-5	0.0	0.0	1.1649033886552755	0.0010076474140933347
66-67	6.297796338083342E-5	0.0	0.0	1.2883087079000186	0.0010076474140933347
68-69	6.297796338083342E-5	0.0	0.0	1.4311742178294393	0.0010076474140933347
70-71	6.297796338083342E-5	0.0	0.0	1.5841791798631741	0.0010076474140933347
72-73	6.297796338083342E-5	0.0	0.0	1.717409061395327	0.0011336033408550015
74-75	6.297796338083342E-5	0.0	0.0	1.8561180257416128	0.0011336033408550015
76-77	6.297796338083342E-5	0.0	0.0	2.0229466507374405	0.0011650923225454183
78-79	6.297796338083342E-5	0.0	0.0	2.216729844060265	0.0011965813042358348
80-81	6.297796338083342E-5	0.0	0.0	2.400688475095679	0.0012280702859262516
82-83	6.297796338083342E-5	0.0	0.0	2.5978724784410687	0.0013225372309975017
84-85	6.297796338083342E-5	0.0	0.0	2.7980164460653576	0.0013225372309975017
86-87	6.297796338083342E-5	0.0	0.0	3.0279804793504708	0.0013540262126879184
88-89	6.297796338083342E-5	0.0	0.0	3.280490623525922	0.0013855151943783352
90-91	6.297796338083342E-5	0.0	0.0	3.550414174576174	0.0013855151943783352
92-93	6.297796338083342E-5	0.0	0.0	3.8067974634995467	0.0015114711211400018
94-95	6.297796338083342E-5	0.0	0.0	4.064566267617298	0.0015744490845208353
96-97	6.297796338083342E-5	0.0	0.0	4.3637430826579475	0.0016689160295920854
98-99	6.297796338083342E-5	0.0	0.0	4.71160186339198	0.0017318939929729187
100-101	6.297796338083342E-5	0.0	0.0	5.074165998575438	0.0017633829746633355
102-103	6.297796338083342E-5	0.0	0.0	5.453513760999889	0.0017633829746633355
104-105	6.297796338083342E-5	0.0	0.0	5.839631654487778	0.0018263609380441688
106-107	6.297796338083342E-5	0.0	0.0	6.259064890604129	0.0018893389014250023
108-109	6.297796338083342E-5	0.0	0.0	6.703846756981265	0.0019838058464962524
110-111	6.297796338083342E-5	0.0	0.0	7.181503120243196	0.002235717700019586
112-113	6.297796338083342E-5	0.0	0.0	7.655412294683967	0.002330184645090836
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3685	0.0	41.848724	1
GGTATCA	2530	0.0	38.1254	1
GTACATG	9215	0.0	35.666763	1
TCAACGC	4385	0.0	35.158215	4
TACATGG	9340	0.0	34.479485	2
CAACGCA	4450	0.0	34.24338	5
AACGCAG	4630	0.0	33.035458	6
ACATGGG	9685	0.0	32.75852	3
TATCAAC	4680	0.0	32.6887	2
CATGGGG	8295	0.0	32.57888	4
ATCAACG	4715	0.0	32.19253	3
ATGGGGG	5285	0.0	31.761484	5
ACGCAGA	5620	0.0	27.204897	7
TGGGGGG	4340	0.0	26.329323	6
CGCAGAG	5850	0.0	26.135303	8
TACGTCT	325	2.3646862E-11	23.806099	6
CTACGTC	480	0.0	23.561832	5
CTCTACG	480	0.0	23.561832	3
TCTACGT	380	0.0	23.496563	4
GCAGAGT	6585	0.0	22.585037	9
>>END_MODULE